1. Maternal protein restriction during early lactation induces changes on neutrophil activation and TNF-alpha production of adult offspring.
- Author
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Silva SV, Garcia-Souza EP, Moura AS, and Barja-Fidalgo C
- Subjects
- Aging, Animals, Chemotaxis, Leukocyte, Diet, Protein-Restricted, Disease Models, Animal, Female, Inflammation Mediators metabolism, Lipopolysaccharides, Liver immunology, Liver metabolism, Male, NF-kappa B metabolism, Neutrophils metabolism, Nitric Oxide metabolism, Nitric Oxide Synthase Type II metabolism, Phagocytosis, Pleurisy chemically induced, Pleurisy immunology, Pleurisy metabolism, Protein-Energy Malnutrition etiology, Protein-Energy Malnutrition metabolism, RNA, Messenger metabolism, Rats, Rats, Wistar, Spleen immunology, Spleen metabolism, Superoxides metabolism, Tumor Necrosis Factor-alpha genetics, Animal Nutritional Physiological Phenomena, Immunity, Innate, Lactation, Maternal Nutritional Physiological Phenomena, Neutrophil Activation, Neutrophils immunology, Protein-Energy Malnutrition immunology, Tumor Necrosis Factor-alpha blood
- Abstract
Maternal nutritional environmental in early life has been often associated with long term effects on the immune response in adulthood. The effects of maternal protein deprivation during early lactation on neutrophil-mediated innate immunity were investigated in adult rats, offspring of dams fed with a protein-free diet during the first days of lactation (PD), and compared to controls (22% protein diet). Inflamed PD animals showed an inhibited neutrophil migration and an impaired recruitment of leukocytes from bone marrow pool to circulation. In resting conditions, blood neutrophils from PD present reduced phagocytic activity, increased production of O(2) (-) and NO, basal iNOS expression and constitutive NF-kappaB activation. Moreover, PD rats also show high circulating levels of TNF-alpha and increased expression of TNF-alpha mRNA in the spleen and liver. The data indicate that maternal nutritional stress can interfere on the innate immune response in adulthood, imprinting permanent alterations on cytokine production and neutrophil activation.
- Published
- 2010
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