1. Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro.
- Author
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Ma J, Guan R, Shen H, Lu F, Xiao C, Liu M, and Kang T
- Subjects
- Antineoplastic Agents adverse effects, Antineoplastic Agents chemistry, Apoptosis drug effects, Caco-2 Cells, Cell Proliferation drug effects, Cell Survival drug effects, Colonic Neoplasms metabolism, Colonic Neoplasms pathology, Drug Stability, Enzyme Stability, Humans, Kinetics, Lactoferrin adverse effects, Lactoferrin chemistry, Lactoferrin ultrastructure, Lipid Peroxidation drug effects, Liposomes, Microscopy, Electron, Transmission, Mitochondria metabolism, Mitochondria pathology, Nanospheres, Neoplasm Proteins metabolism, Particle Size, Reactive Oxygen Species agonists, Reactive Oxygen Species metabolism, Receptors, Cholecystokinin metabolism, Antineoplastic Agents pharmacology, Colonic Neoplasms drug therapy, Lactoferrin pharmacology, Mitochondria drug effects
- Abstract
The anticancer activities of Lactoferrin (Lf) and Lf nanoliposomes in Caco-2 cells were observed in this study, and mitochondrial function (MTT assay), count kit-8(CCK-8), detection of intracellular reactive oxygen species (ROS) and apoptosis induction (AO/EB staining) assays were used to evaluate the anticancer activity. MTT results demonstrated that Lf nanoliposomes and Lf reduced the mitochondrial activity of cells in a manner of dose and time effect, and the viabilities of Caco-2 cell were significantly decreased in vitro following exposure to Lf nanoliposomes at the concentrations of 5 and 10 mg/mL. LDH leakage and ROS significantly increased in cells exposed to Lf nanoliposomes (≥5 mg/mL), while Lf induced ROS only at higher doses (10mg/mL). CCK-8 evaluation of cell proliferation and AO/EB double staining supported the anti-proliferative effects of Lf liposomes. Our findings demonstrated that the presence of Lf nanoliposome is more significant than Lf in inhibiting human tumor cells proliferation. Therefore, it can be concluded that Lf nanoliposomes are a potential therapeutic modality in the management of tumors., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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