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In order to get a comprehensive overview of the effect of land use on soil physical properties, this study was conducted on the selected representative soil samples collected at the territory of Autonomous Province of Vojvodina and included 6 types of soil present in this area. Undisturbed soil samples were taken from 61 localities, from the surface horizon (0 -30 cm) using the Kopetzky cylinders in three replications. The rate of water flow through soil was determined by the constant flow rate method. The velocities of water flow and filtration coefficients were calculated (K-Darcy), while statistical analysis confirmed a positive correlation between soil use and soil permeability. The results showed that soils used for agricultural production have lower permeability compared to soils in forests, meadows, and pastures.