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The behaviour of carbon dioxide (CO2) and methane (CH4) emissions at the surface and below the soil cover in an industrial waste landfill under anaerobic operating conditions was evaluated for six years. This landfill contained gypsum board waste and incineration ash – a practice currently allowed because of a change in Japanese regulations. The CO2 and CH4 fluxes decreased throughout the six years of the survey. Almost all of the survey points exhibited fractions of CH4 in landfill gas emissions of <0.5 (mean values: 0.0–0.1 [surface], 0.0–0.3 [subsurface]) under anaerobic conditions. In addition, a relatively high first-order reaction rate constant for the landfill gas emissions (0.3 year−1) was observed. The landfill leachate showed a relatively high sulphate ion (SO42–) concentration, although other environmental conditions, such as the pH, oxidation–reduction potential and ammonium concentration, were not at levels that could have inhibited CH4 production. These findings suggest that the low fractions could have been related to the lower amounts of CH4 generation caused by competition between methanogens and sulphate-reducing bacteria (SRB). Therefore, SRB could play a major role in the degradation of organic carbon in the landfill. [ABSTRACT FROM AUTHOR]

Abstract: A borehole core from 20 m depth of a Japanese landfill was characterized chemically and microbially. The borehole core sample was typically divided into 5 waste layers; 2.4–4.0 m, 5.7–8.5 m, 9.25–9.6 m, 9.77–14.9 m, and 15.9–17.86 m depths. The waste layers'' ages spanned about 14 years between the bottom and top. Archaeal 16S rRNA gene and eubacterial 16S rRNA gene in the waste samples at their respective levels were 9.8×105–7.2×107 and 1.2×107–7.2×109 copy/g-wet. Similar to populations of viable and culturable bacteria, those populations were high at 7.0 m and 17.5 m depth, but low at 3.0 m depth. The microorganisms'' phenotypes and genotypes were evaluated, respectively, using carbon-utilization tests and by eubacterial 16S rRNA gene based T-RFLP. Low dominance of the VFA-utilizing bacteria in samples and low concentrations of VFAs in all waste layers suggest that the organic decomposition in this landfill site remained. Gamma-proteobacteria dominated the microbial community at 17.5 m depth. Clostridia were detected at 7.0, 11.5, and 17.5 m depths, suggesting strict anaerobic conditions in these deep layers. The Shannon–Weaver diversity index showed lower values at 3.0 m and 11.5 m depth with a T-RF pattern. The diversity index calculated from the carbon-utilization pattern increased slightly with depth at the landfill site. The landfill-site waste layers are expected to be mutually isolated and to form unique microbial communities depending on the buried wastes'' composition, temperature, moisture content, and pressure inside the landfill. [Copyright &y& Elsevier]

• Long term (3 years) CH 4 emissions from field-scale semi-aerobic test cell was studied. • CH 4 emission during wet and dry seasons were systematic measured and compared. • The presence of gas vents reduced average CH 4 emissions to half of that without them. • CH 4 hotspots developed in semi-aerobic cell after rainfall. • Gas vents maintain semi-aerobic conditions when rainwater infiltration is low. Two landfill test cells, with and without gas vents, were used to investigate the effectiveness of passive aeration, through basal leachate pipes, in mitigating methane emissions from municipal solid waste disposal in the tropical climate of Thailand. Surface methane emission rate, as well as methane content in the landfill gas, were determined for a period of three years. The results indicate that the average methane emission rate from the test cell with passive gas vents (42.13 g/t dry wt./d) was about half of that from the test cell without gas vents (90.33 g/t dry wt./d). Methane emission rates from both test cells fluctuated and were influenced by precipitation. The emission rate during the wet period in the test cell with gas vents (61.67 g/t dry wt./d) was 3 times as much as that observed during the dry period (20.95 g/t dry wt./d). The emission rate during the wet period in the test cell without gas vents (120.33 g/t dry wt./d), was twice the value of that observed during the dry period (60.32 g/t dry wt./d). The measurements also revealed the formation of methane hotspots in the test cell with passive vents after rainfall events, leading to higher localized surface emissions. Introduction of gas vents helped reduce methane emissions from solid waste landfills in a tropical region. However, rainfall should be limited to avoid turning semi-aerobic conditions into anaerobic conditions. [ABSTRACT FROM AUTHOR]