1. Optoperforation of single, intact Arabidopsis cells for uptake of extracellular dye-conjugated dextran.
- Author
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LeBlanc ML, Merritt TR, McMillan J, Westwood JH, and Khodaparast GA
- Subjects
- Arabidopsis radiation effects, Dextrans administration & dosage, Drug Carriers administration & dosage, Drug Carriers pharmacokinetics, Fluorescent Dyes administration & dosage, Plant Epidermis radiation effects, Tissue Distribution, Arabidopsis metabolism, Dextrans pharmacokinetics, Electroporation methods, Fluorescent Dyes pharmacokinetics, Lasers, Plant Epidermis metabolism
- Abstract
A plant science research goal is to manipulate single cells in an intact organism in order to study their interactions with neighboring cells. Based on a technique previously demonstrated in isolated plant cells, mammalian cells and cyanobacteria, Arabidopsis epidermal cells were optoperforated to allow for uptake of external cascade blue-labeled dextrans. Adverse organelle responses were determined to be minimal and dye retention was demonstrated for at least 72 hours. This technique overcomes the physical challenges presented by the plant cell wall and demonstrates the feasibility of in situ optoperforation.
- Published
- 2013
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