1. Persistence of parvovirus H-1 DNA in human B- and T-lymphoma cells.
- Author
-
Faisst S, Bartnitzke S, Schlehofer JR, and zur Hausen H
- Subjects
- Burkitt Lymphoma genetics, Cell Transformation, Viral, DNA Replication, Gene Amplification, Humans, Kinetics, Leukemia, B-Cell genetics, Leukemia, T-Cell genetics, Parvoviridae growth & development, Tumor Cells, Cultured, Virus Replication genetics, Burkitt Lymphoma microbiology, DNA, Viral metabolism, Leukemia, B-Cell microbiology, Leukemia, T-Cell microbiology, Parvoviridae genetics, Superinfection genetics
- Abstract
Persisting DNA of parvovirus H-1 could be demonstrated in cells of two human lymphoma cell lines, the Burkitt lymphoma cell line BL2 and the T-cell leukemia cell line Jurkat which survived infection with parvovirus H-1. Persistence of H-1 DNA rendered the cells resistant to a second H-1 infection. This resistance to H-1 superinfection persisted even after loss of H-1 DNA occurring after approximately 150-200 cell generations. Resistance to H-1 superinfection was accompanied by reduced uptake of infectious particles and by a block of H-1 DNA replication. This suggests that persistent H-1 infection leads to modifications of cellular functions involved in the permissivity for H-1.
- Published
- 1990
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