1. Negative regulation of peroxiredoxin 6 (Prdx 6) transcription by nuclear oncoprotein DEK during leukemia cell differentiation.
- Author
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Kim, Kee-Beom, Chae, Yun-Cheol, Han, Arim, Kang, Joo-Young, Jung, Hyeonsoo, Park, Jin Woo, Hahm, Ja Young, Kim, Seryeon, and Seo, Sang-Beom
- Subjects
PEROXIREDOXINS ,ACUTE myeloid leukemia ,CELL differentiation ,CHROMATIN ,IMMUNOPRECIPITATION - Abstract
The oncogene protein DEK is an abundant and ubiquitous nuclear protein with implications in acute myelogenous leukemia, as translocation which results in the formation of a DEK-CAN fusion protein. In a previous study, we have identified that DEK negatively regulated peroxiredoxin 6 (Prdx 6) transcription synergistically with the p65 subunit of NF-κB. In this study, we further investigated DEK-mediated transcriptional regulation of Prdx 6 during leukemia cell differentiation. Using Chromatin Immunoprecipitation analysis and Prdx 6 reporter assays, we found that DEK operated as a negative regulator of Prdx 6 transcription during leukemia cell differentiation. DEK was highly expressed and recruited to Prdx 6 promoter along with p65 and repressed transcription after leukemia cell differentiation. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
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