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2. Additional RNA-Protein Interactions Facilitate in vitro Selection by Ribosome Display.

Author

Inoue, Atsushi, Sawata, Shinya Y., Fujita, Satoshi, and Taira, Kazunari

Subjects
RNA-protein interactions, RNA, PROTEINS, RIBOSOMES, LIBRARIES
Abstract

By introducing specific RNA-protein interaction into ribosome display method, further stabilization of RNA-ribosome-protein complex was achieved. The amount of mRNA of interest recovered after one round of selection correlates with the strength of RNA-protein interaction. This noncovalent but strong interaction should provide a simple and effective strategy for selections of functional proteins from large libraries. [ABSTRACT FROM AUTHOR]

Published
2005
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