7 results on '"Hourioux, Christophe"'
Search Results
2. Hepatitis C virus budding at lipid droplet-associated ER membrane visualized by 3D electron microscopy
- Author
-
Roingeard, Philippe, Hourioux, Christophe, Blanchard, Emmanuelle, and Prensier, Gérard
- Published
- 2008
- Full Text
- View/download PDF
3. Viral sequence variation in chronic carriers of hepatitis C virus has a low impact on liver steatosis.: HCV variability and steatosis
- Author
-
Depla, Marion, D'Alteroche, Louis, Le Gouge, Amélie, Alain, Moreau, Hourioux, Christophe, Meunier, Jean-Christophe, Gaillard, Julien, De Muret, Anne, Bacq, Yannick, Kazemi, Farhad, Avargues, Aurélie, Roch, Emmanuelle, Piver, Eric, Gaudy-Graffin, Catherine, Giraudeau, Bruno, Roingeard, Philippe, Virus, pseudo-virus: Morphogénèse et Antigénicité, Université de Tours-EA3856, Service de gastro-entérologie [CHU Trousseau], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), CIC - Tours, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Biologie Cellulaire, Hôpital Bretonneau-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), PPF Analyse des Systèmes Biologiques (ASB), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Plate-Forme des Microscopies, CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service d'Hépatogastroentérologie, Centre Hospitalier de Blois (CHB), Service de Bactériologie-Virologie, Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau, This work was supported by grants from INSERM-DHOS 'Recherche Translationnelle: Virostéatose' and by the 'Ligue Contre le Cancer' (Loir et Cher and Ile et Vilaine). M.D. was supported by a fellowship from INSERM-Région Centre and the 'Fondation pour la Recherche Médicale'. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., and Université de Tours (UT)-EA3856
- Subjects
MESH: Viral Core Proteins ,viruses ,lipid droplet ,MESH: Amino Acid Sequence ,liver ,MESH: Genotype ,MESH: Biopsy ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,steatosis ,MESH: Hepacivirus ,MESH: Genetic Variation ,MESH: Phylogeny ,MESH: Fatty Liver ,MESH: Aged ,MESH: Humans ,MESH: Middle Aged ,MESH: Molecular Sequence Data ,MESH: Conserved Sequence ,electron microscopy ,MESH: Host-Pathogen Interactions ,MESH: Adult ,MESH: Male ,MESH: Hepatitis C, Chronic ,HCV ,MESH: Viral Nonstructural Proteins ,MESH: Female ,MESH: Liver - Abstract
International audience; Most clinical studies suggest that the prevalence and severity of liver steatosis are higher in patients infected with hepatitis C virus (HCV) genotype 3 than in patients infected with other genotypes. This may reflect the diversity and specific intrinsic properties of genotype 3 virus proteins. We analyzed the possible association of particular residues of the HCV core and NS5A proteins known to dysregulate lipid metabolism with steatosis severity in the livers of patients chronically infected with HCV. We used transmission electron microscopy to quantify liver steatosis precisely in a group of 27 patients, 12 of whom were infected with a genotype 3 virus, the other 15 being infected with viruses of other genotypes. We determined the area covered by lipid droplets in liver tissues and analyzed the diversity of the core and NS5A regions encoded by the viral variants circulating in these patients. The area covered by lipid droplets did not differ significantly between patients infected with genotype 3 viruses and those infected with other genotypes. The core and NS5A protein sequences of the viral variants circulating in patients with mild or severe steatosis were evenly distributed throughout the phylogenic trees established from all the collected sequences. Thus, individual host factors seem to play a much greater role than viral factors in the development of severe steatosis in patients chronically infected with HCV, including those infected with genotype 3 viruses.
- Published
- 2012
- Full Text
- View/download PDF
4. Hepatitis C virus core protein, lipid droplets and steatosis
- Author
-
Roingeard, Philippe, Hourioux, Christophe, Roingeard, Philippe, Virus, pseudo-virus: Morphogénèse et Antigénicité, Université de Tours (UT)-EA3856, and ANRS (Agence Nationale de Recherche sur le SIDA et les hépatites virales), ANR (Agence Nationale de la Recherche / grant Virodynamics), Ligue Contre le Cancer (Comité du Cher)
- Subjects
hepatitis C virus ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,genotype ,HCV ,viral morphogenesis ,steatosis ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,lipid droplet - Abstract
International audience; Lipid droplets are intracellular organelles involved not only in lipid storage, but also in cell signalling and the regulation of intracellular vesicular trafficking. Recent basic studies have suggested that interactions between hepatitis C virus (HCV) core protein and lipid droplets are required for the HCV infection cycle. In infected cells, the HCV core protein is associated with the surface of lipid droplets and the endoplasmic reticulum membranes closely surrounding these droplets, and its self-assembly drives virion budding. This interaction also seems to be directly linked to a virus-induced steatosis, which involves the deposition of triglycerides in the liver and contributes to the progression of fibrosis in patients with chronic hepatitis C. Many clinical studies have reported that virus-induced steatosis is significantly more severe with HCV genotype 3 than with other genotypes, and this phenomenon has been modelled in recent basic studies based on the production of HCV core proteins of various genotypes in vitro. The association of HCV core protein with lipid droplets seems to play a central role in HCV pathogenesis and morphogenesis, suggesting that virus-induced steatosis may be essential for the viral life cycle.
- Published
- 2008
- Full Text
- View/download PDF
5. Hepatitis C virus core protein, lipid droplets and steatosis
- Author
-
Roingeard, Philippe, Hourioux, Christophe, Virus, pseudo-virus: Morphogénèse et Antigénicité, Université de Tours (UT)-EA3856, and ANRS (Agence Nationale de Recherche sur le SIDA et les hépatites virales), ANR (Agence Nationale de la Recherche / grant Virodynamics), Ligue Contre le Cancer (Comité du Cher)
- Subjects
hepatitis C virus ,Fatty Liver ,Organelles ,Liver ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,genotype ,Viral Core Proteins ,HCV ,viral morphogenesis ,steatosis ,lipid droplet ,Humans ,Hepacivirus - Abstract
International audience; Lipid droplets are intracellular organelles involved not only in lipid storage, but also in cell signalling and the regulation of intracellular vesicular trafficking. Recent basic studies have suggested that interactions between hepatitis C virus (HCV) core protein and lipid droplets are required for the HCV infection cycle. In infected cells, the HCV core protein is associated with the surface of lipid droplets and the endoplasmic reticulum membranes closely surrounding these droplets, and its self-assembly drives virion budding. This interaction also seems to be directly linked to a virus-induced steatosis, which involves the deposition of triglycerides in the liver and contributes to the progression of fibrosis in patients with chronic hepatitis C. Many clinical studies have reported that virus-induced steatosis is significantly more severe with HCV genotype 3 than with other genotypes, and this phenomenon has been modelled in recent basic studies based on the production of HCV core proteins of various genotypes in vitro. The association of HCV core protein with lipid droplets seems to play a central role in HCV pathogenesis and morphogenesis, suggesting that virus-induced steatosis may be essential for the viral life cycle.
- Published
- 2007
- Full Text
- View/download PDF
6. The genotype 3-specific hepatitis C virus core protein residue phenylalanine 164 increases steatosis in an in vitro cellular model
- Author
-
Hourioux, Christophe, Patient, Romuald, Morin, Aurélie, Blanchard, Emmanuelle, Alain, Moreau, Trassard, Sylvie, Giraudeau, Bruno, Roingeard, Philippe, Virus, pseudo-virus: Morphogénèse et Antigénicité, Université de Tours-EA3856, CIC - Tours, and Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,genotype ,HCV ,steatosis ,lipid droplet - Abstract
International audience; Background and aims: The prevalence and severity of liver steatosis are higher in patients infected with genotype 3 hepatitis C virus (HCV) than in patients infected with other genotypes. HCV core protein is known to affect lipid metabolism, inducing lipid droplet accumulation both in vitro and in vivo. We used an in vitro cellular model to investigate whether an HCV core protein with residues specific to genotype 3 increased this phenomenon. Methods: Sequence comparisons for HCV core protein domain II, which is known to interact with lipid droplets, identified the phenylalanine (F) residue at position 164 as the only residue specific to genotype 3. We compared the area covered by lipid droplets in sections of cells producing a wild-type genotype 1a HCV core protein with that in cells producing a Y164F mutant protein. Results: Cumulative lipid droplet area was significantly greater in sections of cells producing the Y164F mutant HCV core protein than in cells producing the wild-type protein (p
- Published
- 2007
7. Virus de l'hépatite C et gouttelettes lipidiques
- Author
-
Roingeard, Philippe, Hourioux, Christophe, Virus, pseudo-virus: Morphogénèse et Antigénicité, Université de Tours (UT)-EA3856, Nous remercions pour leur soutien l'Association Nationale pour la Recherche sur le SIDA et les virus des hépatites (ANRS), la Ligue contre le Cancer (Comités de l'Indre & Loire et du Cher), la Région Centre (contrat ESPRI, Equipe Soutenue en Partenariat entre la Région et l'Inserm), and Roingeard, Philippe
- Subjects
endocrine system ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,HCV ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,technology, industry, and agriculture ,steatosis ,lipid droplet ,lipids (amino acids, peptides, and proteins) ,complex mixtures ,eye diseases - Abstract
Hepatitis C virus and lipid droplets
- Published
- 2007
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.