Search

Russula vinosa Lindbl (RVL) is a multifunctional edible fungus that has recently gained widespread attention due to its numerous nutritious, delicious, and medicinal properties. However, little attention has been directed toward analyzing comprehensive phytochemical profiling and bioactivity in RVL. This study aimed to evaluate the phytochemical and medicinal profiling of RVL systematically. Herein, we characterized global RVL phytochemical profiling using the LC/GC-MS-based multiomics strategy through initial methodological optimization. A total of 633 metabolites were identified using untargeted multiomics, with fatty acids, amino acids, polyphenols, terpenoids, organic acids, sugars, and phospholipids as the primary metabolites. Polyphenols served as the main active components by quantitative analysis and antioxidant evaluation, impling that they may be related to antitumor effects. Moreover, based on phytochemical profiling, the potential gene targets and mechanisms of anticancer properties of RVL were explored through network pharmacology. The significance of this study lies in expanding our understanding of the phytochemical and bioactive components of RVL and providing references for its further utilization and exploitation. Therefore, RVL could be an excellent raw material for the food, nutraceutical, and pharmaceutical industries due to its richness in nutrients and bioactive ingredients. • Initial methodological optimization to establish reliable analytical method. • Combining metabolomics and lipidomics to reveal global phytochemicals of RVL. • Polyphenols are the main bioactives of RVL with good antioxidant activity. • Using network pharmacology to explore targets and mechanism of RVL' antitumor. [ABSTRACT FROM AUTHOR]

Colorectal advanced adenoma (CAA) is a key precancerous lesion of colorectal cancer (CRC), and early diagnosis can lessen CRC morbidity and mortality. Although abnormal lipid metabolism is associated with the development of CRC, there are no studies on the biomarkers and mechanism of lipid metabolism linked to CAA carcinogenesis. Hence, we performed a lipidomics study of serum samples from 46 CAA, and 50 CRC patients by the ultra high-performance liquid chromatography tandem high resolution mass spectrometry (UHPLC-HRMS) in both electrospray ionization (ESI) modes. Differential lipids were selected by univariate and multivariate statistics analysis, and their diagnostic performance was evaluated using a receiver operating characteristic curve (ROC) analysis. Combining P < 0.05 and variable importance in projection (VIP) > 1, 59 differential lipids were obtained totally. Ten of them showed good discriminant ability for CAA and CRC (AUC > 0.900). Especially, the lipid panel consisting of PC 44:5, PC 35:6e, and SM d40:3 showed the highest selection frequency and outperformed (AUC = 0.952). Additionally, phosphatidylcholine (PC) and sphingomyelin (SM) were the main differential and high-performance lipids. In short, this is the first study to explore the biomarkers and mechanism for CAA-CRC sequence with large-scale serum lipidomics. The findings should provide valuable reference and new clues for the development of diagnostic and therapeutic strategies of CRC. [Display omitted] • Obvious differences in serum lipid profiles between CAA and CRC. • Discovery of biomarkers of CAA carcinogenesis by non-targeted lipidomics. • Disorders of PC and SM metabolism are associated with CAA cancerization. [ABSTRACT FROM AUTHOR]