1. Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity.
- Author
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Sarabia LD, Boughton BA, Rupasinghe T, Callahan DL, Hill CB, and Roessner U
- Subjects
- Ceramides analysis, Chromatography, Liquid methods, Gene Expression Regulation, Plant, Glycerophospholipids analysis, Hordeum drug effects, Hordeum genetics, Ions metabolism, Lipid Metabolism genetics, Metabolome, Metabolomics, Plant Roots drug effects, Plant Roots genetics, Salt Stress genetics, Salts pharmacology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Tandem Mass Spectrometry methods, Hordeum metabolism, Lipidomics methods, Lipids analysis, Plant Roots metabolism, Salinity, Salt Stress physiology
- Abstract
Salinity-induced metabolic, ionic, and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short-term salt stress. We used a combination of liquid chromatography-tandem mass spectrometry, inductively coupled plasma mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry imaging, and reverse transcription - quantitative real time polymerase chain reaction platforms to examine the molecular signatures of lipids, ions, and transcripts in three anatomically different seminal root tissues before and after salt stress. We found significant changes to the levels of major lipid classes including a decrease in the levels of lysoglycerophospholipids, ceramides, and hexosylceramides and an increase in the levels of glycerophospholipids, hydroxylated ceramides, and hexosylceramides. Our results revealed that modifications to lipid and transcript profiles in plant roots in response to a short-term salt stress may involve recycling of major lipid species, such as phosphatidylcholine, via resynthesis from glycerophosphocholine., (© 2019 John Wiley & Sons Ltd.)
- Published
- 2020
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