4 results on '"Malo, Madhu S."'
Search Results
2. Intestinal alkaline phosphatase inhibits the proinflammatory nucleotide uridine diphosphate.
- Author
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Moss, Angela K., Hamarneh, Sulaiman R., Mohamed, Mussa M. Rafat, Ramasamy, Sundaram, Yammine, Halim, Patel, Palak, Kaliannan, Kanakaraju, Alam, Sayeda N., Muhammad, Nur, Moaven, Omeed, Teshager, Abeba, Malo, Nondita S., Narisawa, Sonoko, Millán, José Luis, Warren, H. Shaw, Hohmann, Elizabeth, Malo, Madhu S., and Hodin, Richard A.
- Subjects
INTESTINAL physiology ,ALKALINE phosphatase ,NUCLEOTIDES ,URIDINE diphosphate ,INFLAMMATORY bowel diseases ,CELL culture - Abstract
First published January 10, 2013; doi:10.1152/ajpgi.00455.2012.--Uridine diphosphate (UDP) is a proinflammatory nucleotide implicated in inflammatory bowel disease. Intestinal alkaline phosphatase (IAP) is a gut mucosal defense factor capable of inhibiting intestinal inflammation. We used the malachite green assay to show that IAP dephosphorylates UDP. To study the anti-inflammatory effect of IAP, UDP or other proinflammatory ligands (LPS, flagellin, Pam3Cys, or TNF-) in the presence or absence of IAP were applied to cell cultures, and IL-8 was measured. UDP caused dose-dependent increase in IL-8 release by immune cells and two gut epithelial cell lines, and IAP treatment abrogated IL-8 release. Costimulation with UDP and other inflammatory ligands resulted in a synergistic increase in IL-8 release, which was prevented by IAP treatment. In vivo, UDP in the presence or absence of IAP was instilled into a small intestinal loop model in wild-type and IAP-knockout mice. Luminal contents were applied to cell culture, and cytokine levels were measured in culture supernatant and intestinal tissue. UDP-treated luminal contents induced more inflammation on target cells, with a greater inflammatory response to contents from IAP-KO mice treated with UDP than from WT mice. Additionally, UDP treatment increased TNF-levels in intestinal tissue of IAP-KO mice, and cotreatment with IAP reduced inflammation to control levels. Taken together, these studies show that IAP prevents inflammation caused by UDP alone and in combination with other ligands, and the anti-inflammatory effect of IAP against UDP persists in mouse small intestine. The benefits of IAP in intestinal disease may be partly due to inhibition of the proinflammatory activity of UDP. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
3. Identification of specific targets for the gut mucosal defense factor intestinal alkaline phosphatase.
- Author
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Chen, Kathryn T., Malo, Madhu S., Moss, Angela K., Zeller, Skye, Johnson, Paul, Ebrahimi, Farzad, Mostafa, Golam, Alam, Sayeda N., Ramasamy, Sundaram, Warren, H. Shaw, Hohmann, Elizabeth L., and Hodin, Richard A.
- Subjects
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ALKALINE phosphatase , *CECUM , *CELL culture , *INTERLEUKIN-8 , *BACTERIA , *PHOSPHATASES - Abstract
Intestinal alkaline phosphatase (IAP) is a small intestinal brush border enzyme that has been shown to function as a gut mucosal defense factor, but its precise mechanism of action remains unclear. We investigated the effects of IAP on specific bacteria and bacterial components to determine its molecular targets. Purulent fluid from a cecal ligation and puncture model, specific live and heat-killed bacteria (Escherichia coli, Salmonella typhimurium, and Listeria monocytogenes), and a variety of proinflammatory ligands (LPS, CpG DNA, Pam-3-Cys, flagellin, and TNF) were incubated with or without calf IAP (cIAP). Phosphate release was determined by using a malachite green assay. The various fluids were applied to target cells (THP-1, parent HT-29, and IAP-expressing HT-29 cells) and IL-8 secretion measured by ELISA. cIAP inhibited IL-8 induction by purulent fluid in THP-1 cells by >35% (P < 0.005). HT29-IAP cells had a reduced IL-8 response specifically to gram-negative bacteria; >90% reduction compared with parent cells (P < 0.005). cIAP had no effect on live bacteria but attenuated IL-8 induction by heat-killed bacteria by >40% (P < 0.005). cIAP exposure to LPS and CpG DNA caused phosphate release and reduced IL-8 in cell culture by >50% (P < 0.005). Flagellin exposure to cIAP also resulted in reduced IL-8 secretion by >40% (P < 0.005). In contrast, cIAP had no effect on TNF or Pam-3-Cys. The mechanism of IAP action appears to be through dephosphorylation of specific bacterial components, including LPS, CpG DNA, and flagellin, and not on live bacteria themselves, IAP likely targets these bacterially derived molecules in its role as a gut mucosal defense factor. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
4. Phosphate Groups in the Lipid A Moiety Determine the Effects of LPS on Hepatic Stellate Cells: A Role for LPS-Dephosphorylating Activity in Liver Fibrosis.
- Author
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Schippers, Marlies, Post, Eduard, Eichhorn, Ilse, Langeland, Jitske, Beljaars, Leonie, Malo, Madhu S., Hodin, Richard A., Millán, José Luis, Popov, Yury, Schuppan, Detlef, and Poelstra, Klaas
- Subjects
LIVER cells ,MOIETIES (Chemistry) ,LIVER ,ALKALINE phosphatase ,FIBROSIS ,LIPIDS - Abstract
Alkaline phosphatase (AP) activity is highly upregulated in plasma during liver diseases. Previously, we demonstrated that AP is able to detoxify lipopolysaccharide (LPS) by dephosphorylating its lipid A moiety. Because a role of gut-derived LPS in liver fibrogenesis has become evident, we now examined the relevance of phosphate groups in the lipid A moiety in this process. The effects of mono-phosphoryl and di-phosphoryl lipid A (MPLA and DPLA, respectively) were studied in vitro and LPS-dephosphorylating activity was studied in normal and fibrotic mouse and human livers. The effects of intestinal AP were studied in mice with CCL4-induced liver fibrosis. DPLA strongly stimulated fibrogenic and inflammatory activities in primary rat hepatic stellate cells (rHSCs) and RAW264.7 macrophages with similar potency as full length LPS. However, MPLA did not affect any of the parameters. LPS-dephosphorylating activity was found in mouse and human livers and was strongly increased during fibrogenesis. Treatment of fibrotic mice with intravenous intestinal-AP significantly attenuated intrahepatic desmin
+ − and αSMA+ −HSC and CD68+ − macrophage accumulation. In conclusion, the lack of biological activity of MPLA, contrasting with the profound activities of DPLA, shows the relevance of LPS-dephosphorylating activity. The upregulation of LPS-dephosphorylating activity in fibrotic livers and the protective effects of exogenous AP during fibrogenesis indicate an important physiological role of intestinal-derived AP during liver fibrosis. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
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