1. Matrix-free DNP-enhanced NMR spectroscopy of liposomes using a lipid-anchored biradical.
- Author
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Fernández-de-Alba C, Takahashi H, Richard A, Chenavier Y, Dubois L, Maurel V, Lee D, Hediger S, and De Paëpe G
- Subjects
- Cyclic N-Oxides chemistry, Lipid Bilayers chemistry, Nuclear Magnetic Resonance, Biomolecular, Propanols chemistry, Free Radicals chemistry, Liposomes chemistry
- Abstract
Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
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