1. Impact of Sod on the expression of stress-related genes in Listeria monocytogenes 4b G with/without paraquat treatment.
- Author
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Suo Y, Liu Y, Zhou X, Huang Y, Shi C, Matthews K, and Shi X
- Subjects
- Bacterial Proteins metabolism, Gene Expression Regulation, Bacterial drug effects, Gene Knockout Techniques, Listeria monocytogenes drug effects, Listeria monocytogenes enzymology, Microbial Viability drug effects, Superoxide Dismutase metabolism, Transcription, Genetic, Bacterial Proteins genetics, Listeria monocytogenes genetics, Oxidants pharmacology, Oxidative Stress, Paraquat pharmacology, Superoxide Dismutase genetics
- Abstract
Listeria monocytogenes is a foodborne pathogen that causes listeriosis. Paraquat can generate reactive oxygen species (ROS) in cells, which results in oxidative stress. It was first shown that 1 mM of paraquat inhibited the growth rate of a superoxide dismutase (sod)-deletion mutant (∆sod) generated from L. monocytogenes 4b G but not in the wild-type, and induced the expression of other resistance genes (kat, fri, perR, sigB, and recA) as well as sod in the wild type. Interestingly, without paraquat treatment the expression of all the 5 genes were repressed in ∆sod compared to the wild type, while the expression of recA triggering SOS response, a global response to DNA damage, was increased in ∆sod in the presence of 1 mM paraquat. Taken together, these results suggest that SOD plays a central role in oxidant defense of L. monocytogenes 4b G, and SOS probably significantly impacts ∆sod survival under oxidative stress., (© 2014 Institute of Food Technologists®)
- Published
- 2014
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