6 results on '"Pham, B.-N."'
Search Results
2. Association of idiopathic hepatic sinusoidal dilatation with the immunological features of the antiphospholipid syndrome.
- Author
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Saadoun D, Cazals-Hatem D, Denninger MH, Boudaoud L, Pham BN, Mallet V, Condat B, Brière J, and Valla D
- Subjects
- Adult, Antibodies, Anticardiolipin blood, Antibodies, Antinuclear blood, Biopsy, Dilatation, Pathologic immunology, Dilatation, Pathologic pathology, Female, Humans, Liver pathology, Lupus Coagulation Inhibitor blood, Male, Middle Aged, Antiphospholipid Syndrome diagnosis, Liver blood supply
- Abstract
Background: Isolated sinusoidal dilatation is an uncommon hepatic lesion and the cause is largely unknown., Objective: To investigate whether prothrombotic disorders or perisinusoidal cell changes could be involved in pure idiopathic hepatic sinusoidal dilatation (HSD)., Methods: Evaluation for associated conditions, prothrombotic disorders, and studies of hepatic perisinusoidal cell activation in consecutive patients, seen between 1993 and 2002, with isolated sinusoidal dilatation unrelated to outflow block, sinusoidal infiltration, or hepatic granulomas., Results: Among 11 patients, associated conditions were prothrombotic disorders (n = 5) and oral contraceptive use (n = 3). Prothrombotic disorders were polycythemia vera (n = 1) and anticardiolipin antibodies combined with lupus anticoagulant (n = 4). No genetic thrombophilia factor was found. Of four patients with lupus anticoagulant, three had antinuclear factors and high serum levels of anticardiolipin antibodies at repeated testing. There was no evidence of intrahepatic or extrahepatic thrombosis in any of the patients. Sinusoidal dilatation was marked in six of 11 patients (54%), including two patients with antiphospholipid antibodies. Activated perisinusoidal cells were only found around markedly dilated sinusoids., Conclusion: Idiopathic pure HSD is frequently associated with the immunological features of the antiphospholipid syndrome. Therefore, finding pure HSD in a liver biopsy specimen should prompt the search for antiphospholipid antibodies.
- Published
- 2004
- Full Text
- View/download PDF
3. Eotaxin expression and eosinophil infiltrate in the liver of patients with drug-induced liver disease.
- Author
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Pham BN, Bemuau J, Durand F, Sauvanet A, Degott C, Prin L, and Janin A
- Subjects
- Acetaminophen adverse effects, Adult, Blood Cell Count, Chemical and Drug Induced Liver Injury blood, Chemokine CCL11, Chemokine CCL5 metabolism, Female, Humans, Male, Middle Aged, Reference Values, Chemical and Drug Induced Liver Injury metabolism, Chemical and Drug Induced Liver Injury pathology, Chemokines, CC, Cytokines metabolism, Eosinophils pathology, Liver metabolism, Liver pathology
- Abstract
Background: Drug-induced liver disease is due to intrinsic or idiosyncratic hepatotoxins. Liver parenchyma is then infiltrated by immunocompetent cells. Eosinophils are primarily tissue leukocytes which are attracted into tissues by various chemoattractants, including chemokines. The aim of this study was to study eosinophils in the livers of patients with drug-induced liver disease., Methods: Immunohistochemical studies with antibodies against eosinophil cationic proteins (major basic protein, eosinophil-derived neurotoxin and eosinophil cationic protein), cytokines (interleukin 5 (IL-5), interleukin 3 (IL-3) and granulocyte-macrophage colony stimulating factor (GM-CSF)) and chemokines (eotaxin and RANTES (Regulated upon Activation Normal T cell Expressed and Secreted)) were performed to assess the in situ activation of the liver-infiltrating eosinophils of 14 patients with drug-induced liver disease and 19 controls., Results: Eosinophils were only observed in patients with drug-induced liver disease. Eosinophils were morphologically normal when hepatitis was due to paracetamol whereas eosinophils had granular changes when hepatitis was due to an idiosyncratic hepatotoxin. Eotaxin was detected in all patients with drug-induced liver disease, whereas RANTES was detected in three of them. IL-5, IL-3 or GM-CSF were not detected., Conclusions: In patients with drug-induced liver disease, the recruitment of eosinophils in the liver may depend on eotaxin expression. Eosinophil changes may vary according to the type of drug.
- Published
- 2001
- Full Text
- View/download PDF
4. Relationship between the effector T-cell response and viremia in symptomatic chronic hepatitis C.
- Author
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Mosnier JF, Pham BN, Scoazec JY, Dubois S, Marcellin P, Martinot-Peignoux M, Degos F, Erlinger S, and Degott C
- Subjects
- Adult, Antigens, CD immunology, Female, Genotype, Hepatitis C, Chronic pathology, Humans, Immunohistochemistry, Leukocyte Common Antigens immunology, Liver pathology, Liver virology, Male, Middle Aged, RNA, Viral analysis, T-Lymphocytes pathology, Viremia immunology, Hepatitis C, Chronic immunology, Liver immunology, T-Lymphocytes immunology
- Abstract
Objective: The purpose of this study was to assess the relationship between hepatitis C virus (HCV) viremia, HCV genotype, histologic activity index, and intrahepatic densities of immunocompetent lymphoid cells in chronic hepatitis C., Method: Liver biopsy specimens from patients with chronic hepatitis and anti-HCV antibodies, 5 with low-level HCV viremia and 26 with high-level HCV viremia, were studied. Sections of snap-frozen specimens were immunostained with monoclonal antibodies directed against different subsets of B cells, follicular reticulum cells, and T cells, including CD45RA-naive cells and CD45RO-primed cells. The densities of each subset of lymphoid cells were calculated with a computerized image analyzer. Hepatitis C virus RNA was measured with a quantitative branched DNA signal amplification assay, and HCV genotypes were determined with the line probe assay., Results: A statistical correlation was found between the level of HCV viremia and the histologic activity index (P = .002). There were also significant correlations between the densities of CD3+ T cells and CD45RO+-primed cells in the liver and the histologic activity index and (P = .024 and P = .005, respectively), and significant correlations were found between the densities of CD3+ T cells, CD4+ T cells, CD8+ T cells, and CD45RO+-primed cells and HCV viremia (P = .005, P = .03, P = .03, and P = .02, respectively). Whatever the viremia status, CD45RO-primed cells were more numerous than CD45RA-naive cells in lobules and portal tracts. The CD45RO/CD45RA ratio was significantly higher when intraportal lymphoid aggregates were present. Eight patients were infected with genotype 1b, and 11 were infected with genotype 3b. There were no statistical differences in the histologic activity index, viremia, and density of immunocompetent cells in the liver between these two HCV genotypes., Conclusions: The results of this study suggest that the density of antigen-primed effector T cells in the liver of symptomatic patients with chronic hepatitis C is adapted to HCV viremia but is independent of the occurrence of genotypes 1b or 3a, the most frequent genotypes found in France.
- Published
- 1998
5. Relationship between composition of lymphoid cell infiltrates in the liver and replication status in chronic hepatitis B: an immunohistochemical study.
- Author
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Mosnier JF, Pham BN, Walker F, Dubois S, Degos F, Erlinger S, and Degott C
- Subjects
- Adult, Aged, B-Lymphocyte Subsets classification, Dendritic Cells pathology, Female, Hepatitis B virology, Hepatitis B virus physiology, Hepatitis, Chronic virology, Humans, Immunohistochemistry methods, Killer Cells, Natural pathology, Liver virology, Male, Middle Aged, T-Lymphocyte Subsets classification, Virus Replication physiology, B-Lymphocyte Subsets pathology, Hepatitis B pathology, Hepatitis B virus isolation & purification, Hepatitis, Chronic pathology, Liver pathology, T-Lymphocyte Subsets pathology
- Abstract
The purpose of this in situ immunophenotyping study was to assess the nature of lymphoid cells involved in chronic hepatitis B with various levels of replication. Replicative status was studied in 25 patients with chronic hepatitis B. Different subsets of T cells, B cells, natural killer cells, and follicular dendritic cells were counted with a computerized image analyzer. Twelve patients had chronic hepatitis B with a high level of replication, nine without replication, and four with a low level of replication. The number of CD3+ T cells was significantly higher in the liver of patients with replication. In all cases, CD8+ T cells were the most numerous cells in the lobules and CD4+ T cells were the most numerous cells in the portal tract. Intra-portal lymphoid follicles were observed in 33% of patients with a high level of replication. The CD57+ natural killer cells were more numerous in patients with a low level of replication. This study suggests that in immunocompetent patients, the intensity and type of hepatic immune response are directly determined by the HBV replication.
- Published
- 1996
- Full Text
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6. Flow cytometry CD4+/CD8+ ratio of liver-derived lymphocytes correlates with viral replication in chronic hepatitis B.
- Author
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Pham BN, Mosnier JF, Walker F, Njapoum C, Bougy F, Degott C, Erlinger S, Cohen JH, and Degos F
- Subjects
- Adult, Aged, Antigens, CD immunology, CD4-Positive T-Lymphocytes immunology, Chronic Disease, DNA, Viral analysis, Female, Flow Cytometry, Hepatitis B microbiology, Humans, Liver microbiology, Male, Middle Aged, Receptors, Antigen, T-Cell, gamma-delta immunology, T-Lymphocytes, Regulatory immunology, Virus Replication immunology, CD4-CD8 Ratio, Hepatitis B immunology, Hepatitis B virus physiology, Liver immunology
- Abstract
T lymphocytes have been assumed to play an essential role in tissue injury in patients with chronic hepatitis B. As hepatitis B virus (HBV) is considered as a major factor controlling liver inflammation, we assessed whether a particular T lymphocyte subset could be preferentially detected in the liver in accordance with viral replication. Liver-derived lymphocytes and peripheral blood lymphocytes were analysed by flow cytometry in 21 patients with histologically confirmed chronic hepatitis B without cirrhosis. Viral replication was quantified by hybridization of serum HBV DNA. Eleven patients exhibited an active viral replication with serum HBV DNA ranging from 10 to 388 pg/ml at the time of the liver biopsy, whereas 10 patients had no detectable serum HBV DNA. In patients exhibiting viral replication, CD4+/CD8+ ratios of liver-derived lymphocytes were significantly higher (P < 0.05) than those obtained in patients without viral replication. In contrast, the percentage of T cells expressing the gamma/delta receptor and that of CD2+/CD57+ cells were similar in both groups of patients. Furthermore, in patients exhibiting viral replication, CD4+CD8+ ratios of liver-derived lymphocytes correlated with serum HBV DNA levels (P < 0.001). No relationship between CD4+/CD8+ ratio of liver-derived and peripheral blood lymphocytes was observed. Our data indicate that, in patients with chronic hepatitis B, the CD4+/CD8+ ratio of liver-derived lymphocytes correlates with viral replication. This suggests that in situ helper/inducer CD4+ T lymphocytes may positively regulate the cytotoxic T cell activity in patients with HBV-related chronic hepatitis.
- Published
- 1994
- Full Text
- View/download PDF
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