9 results on '"Zhang, Xuewu"'
Search Results
2. Ginsenoside compound K induces mitochondrial apoptosis in human hepatoma cells through Bclaf1-mediated modulation of ERK signaling.
- Author
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Chen, Jiaxin, Sun, Manqing, Cui, Xinmu, and Zhang, Xuewu
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GINSENOSIDES ,MITOCHONDRIA ,HEPATOCELLULAR carcinoma ,APOPTOSIS ,TRANSCRIPTION factors ,CELL proliferation - Abstract
Compound K (CK) is the metabolite and final active ingredient of diol-type ginsenosides. In this study, we investigated the effect of CK on mitochondrial apoptosis in SMMC-7721 and BEL-7404 cells and the regulatory mechanism through in vitro and in vivo experiments. The results demonstrated that CK inhibited Hepatocellular carcinoma (HCC) cells proliferation and arrested the cells in G0/G1 phase. CK induces mitochondrial apoptosis in HCC cells and inhibited p-ERK expression. Bcl-2 associated transcription factor 1 (Bclaf1) was distributed in the nucleus and cytoplasm, and CK inhibited its expression. Treatment of a nude mouse xenograft model bearing SMMC-7721 cells with CK decreased the expression of Bclaf1, p-ERK, and Bcl-2 but increased that of Bax. In summary, ginsenoside CK downregulated Bclaf1 expression, inhibited the activation of the ERK pathway, and triggered mitochondrial apoptosis in HCC. These findings uncovered a potential therapeutic strategy leveraging the anti-tumor effects of CK against HCC. [ABSTRACT FROM AUTHOR]
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- 2022
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3. Curcumin inhibits invasion and metastasis of human hepatoma cells through Bclaf1-mediated Wnt/β-catenin signalling.
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Zhao, Zhongwei, Su, Jielin, Zhao, Jiaqi, Chen, Jiaxin, Cui, Xinmu, Sun, Manqing, and Zhang, Xuewu
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CURCUMIN ,WNT signal transduction ,HEPATOCELLULAR carcinoma ,METASTASIS ,INTRAPERITONEAL injections ,CELLULAR signal transduction ,GENE targeting - Abstract
Curcumin, a kind of natural compound extracted from the rhizome of Zingiberaceae such as turmeric, has many pharmacological effects such as anti-cancer effects. This study investigated the effect of curcumin on the invasion and metastasis of hepatocellular carcinoma (HCC) cell lines HepG2 and SK-Hep-1 through the Wnt/β-catenin signalling pathway and the regulatory mechanism of Bcl-2-associated transcription factor 1 (Bclaf1). Curcumin significantly inhibited the migration and invasion of HepG2 and SK-Hep-1 cells and inhibited the Wnt/β-catenin signalling pathway and reduced Bclaf1 expression in human hepatoma cells. In nude mice, intraperitoneal injection of curcumin significantly inhibited the growth of subcutaneously transplanted tumours and reduced lung metastasis of the tumour cells, downregulated the expression of Bclaf1, and inhibited the Wnt/β-catenin pathway. This study suggests that curcumin is a novel candidate drug to prevent cancer metastasis and that Bclaf1 is a new gene target related to the proliferation, invasion, and metastasis of hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Liver organoids: an in vitro 3D model for liver cancer study.
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Dong, Renshun, Zhang, Bixiang, and Zhang, Xuewu
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LIVER cancer ,ORGANOIDS ,HEPATITIS B ,HEPATITIS B virus ,LIVER - Abstract
Primary liver cancer (PLC) is the second leading cause of cancer mortality worldwide, and its morbidity unceasingly increases these years. Hepatitis B virus (HBV) infection accounted for approximately 50% of hepatocellular carcinoma (HCC) cases globally in 2015. Due to the lack of an effective model to study HBV-associated liver carcinogenesis, research has made slow progress. Organoid, an in vitro 3D model which maintains self-organization, has recently emerged as a powerful tool to investigate human diseases. In this review, we first summarize the categories and development of liver organoids. Then, we mainly focus on the functions of culture medium components and applications of organoids for HBV infection and HBV-associated liver cancer studies. Finally, we provide insights into a potential patient-derived organoid model from those infected with HBV based on our study, as well as the limitations and future applications of organoids in liver cancer research. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa.
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Wang, Xiaoqin and Zhang, Xuewu
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CHLORELLA pyrenoidosa ,POLYPEPTIDES ,ANTINEOPLASTIC agents ,GEL permeation chromatography ,LIVER cancer ,FUNCTIONAL foods ,GREEN algae - Abstract
Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:681-687, 2013 [ABSTRACT FROM AUTHOR]
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- 2013
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6. Ginsenoside Compound K Regulates HIF-1α-Mediated Glycolysis Through Bclaf1 to Inhibit the Proliferation of Human Liver Cancer Cells.
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Zhang, Silin, Zhang, Meilan, Chen, Jiaxin, Zhao, Jiaqi, Su, Jielin, and Zhang, Xuewu
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GLYCOLYSIS ,LIVER cells ,LIVER cancer ,CANCER cells ,POSITRON emission tomography ,CANCER cell proliferation - Abstract
This study aimed to demonstrate that ginsenoside compound K (20 (S)-ginsenoside CK; CK) downregulates Bcl-2-associated transcription factor 1 (Bclaf1), which inhibits the hypoxia-inducible factor-1α (HIF-1α)-mediated glycolysis pathway to inhibit the proliferation of liver cancer cells. Treatment of hepatoma cells (Bel-7404 and Huh7) under hypoxic conditions with different concentrations of CK showed that CK inhibited the proliferation of hepatoma cells in a time- and concentration-dependent manner; furthermore, the ability of the cells to form colonies was reduced, and cell growth was blocked in the G0/G1 phase. CK promoted the degradation of HIF-1α ubiquitination in liver cancer cells by regulating the expression of HIF-1α and related ubiquitination proteins; moreover, it reduced the activity of key enzymes involved in glycolysis, the pressure of cellular glycolysis, and the rate of real-time ATP production, thereby inhibiting the glycolysis pathway. It also decreased the expression of Bclaf1 in hypoxic liver cancer cells and thus reduced the ability of Bclaf1 to bind to HIF-1α. CK treatment of Bel-7404 and Huh7 cells with CRISPR/Cas9-engineered knock out of Bclaf1 gene under hypoxic conditions further suppressed the expression of HIF-1α, promoted HIF-1α ubiquitination, and inhibited the glycolysis pathway. In a rat model of primary liver cancer induced by diethylnitrosamine, positron emission tomography and computed tomography scans showed that after CK administration, tumor tissue volumes were reduced and glucose uptake capacity decreased. Increased Bclaf1 and HIF-1α expression promoted the ubiquitination of HIF-1α and inhibited the glycolysis pathway, thereby inhibiting the proliferation of liver cancer cells. In summary, this study confirmed by in vitro and in vivo experiments that in hypoxic liver cancer cells CK downregulates the expression of Bclaf1, inhibits the HIF-1α-mediated glycolysis pathway, and inhibits cell proliferation, suggesting that the CK-mediated effects on Bclaf1 may represent a novel therapeutic approach for the treatment of liver cancer patients. [ABSTRACT FROM AUTHOR]
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- 2020
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7. 14–3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.
- Author
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Song, Jia, Zhang, Xiaochao, Liao, Zhibin, Liang, Huifang, Chu, Liang, Dong, Wei, Zhang, Xuewu, Ge, Qianyun, Liu, Qiumeng, Fan, Pan, Zhang, Zhanguo, and Zhang, Bixiang
- Subjects
HEME oxygenase ,LIVER cancer ,CELL proliferation ,NEOPLASTIC cell transformation ,IMMUNOFLUORESCENCE - Abstract
Background: Heme oxygenase 1 (HO-1) has been reported to be very important in the pathogenesis or progression of multiple types of cancer. Identification of novel hmox1 binding proteins may reveal undefined oncogenes, tumor suppressors, signaling pathways, and possible treatment targets. Methods: Immunoprecipitation and mass spectrometry analyses were used to identify novel regulators of HO-1. The association of the 14–3-3ζ protein with HO-1 and modulation of the stability of HO-1 were investigated by co-immunoprecipitation, immunofluorescence, western blotting, and quantitative RT-PCR. Degradation and in vivo ubiquitination assays were utilized to examine whether 14–3-3ζ stabilizes the HO-1 protein by inhibiting its ubiquitination. The effect of 14–3-3ζ on proliferation was investigated by function assays conducted in vitro using the CCK-8 and colony formation assays and in vivo in a xenograft mouse model. The biological functions of the 14–3-3ζ/HO-1 axis were demonstrated by western blotting and rescue experiments. Using gain-of-function and loss-of-function strategies, we further clarified the impact of 14–3-3ζ/HO-1 complex on the signal transducers and activators of transcription 3 (STAT3) signaling pathway in cancer cells. Results: We identified 14–3-3ζ as a novel HO-1 binding protein. The binding inhibited the ubiquitination and proteasome-mediated degradation of HO-1, thus facilitating its stabilization. Enforced expression of 14–3-3ζ significantly promoted cell proliferation in vitro, as well as tumorigenesis in vivo, while 14–3-3ζ knockdown had opposite effects. The data indicated that 14–3-3ζ can stabilize HO-1 expression and thus influence cancer cell proliferation. We further demonstrated the involvement of the STAT3 pathway in 14–3-3ζ/HO-1 regulation of hepatocellular carcinoma cell proliferation. Conclusions: Collectively, these data show that 14–3-3ζ regulates the stability of HO-1 to promote cancer cell proliferation and STAT3 signaling activation. The data establish the 14–3-3ζ-HO-1-STAT3 axis as an important regulatory mechanism of cancer cell growth and implicate HO-1 and 14–3-3ζ as potential therapeutic targets in hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]
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- 2019
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8. Compound K Induces Endoplasmic Reticulum Stress and Apoptosis in Human Liver Cancer Cells by Regulating STAT3.
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Zhang, Xuan, Zhang, Silin, Sun, Qitong, Jiao, Wenjun, Yan, Yan, and Zhang, Xuewu
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ENDOPLASMIC reticulum ,LIVER cancer ,CANCER cells ,GINSENOSIDES ,APOPTOSIS - Abstract
The ginsenoside compound K (20-
O -β-d-glucopyranosyl-20(S )-protopanaxadiol; CK) is an intestinal bacterial metabolite of ginseng protopanaxadiol saponin that has been reported to induce apoptosis in many cancer cells; however, the precise mechanisms of its activity in human hepatocellular carcinoma (HCC) cells remain unclear. Herein, we demonstrated that CK inhibited the growth and colony formation of HepG2 and SMMC-7721 cells, phenotypes that were mediated by inducing apoptosis. Meanwhile, CK showed lower toxicity in normal hepatoma cells. After treating HepG2 and SMMC-7721 cells with CK, p-STAT3 levels decreased, the three branches of the unfolded protein response were activated, and levels of endoplasmic reticulum stress (ERS)-related proteins were increased. We also revealed that CK decreased the DNA-binding capacity of STAT3. Moreover, silencing STAT3 with CRISPR/Cas9 technology enhanced CK-induced ERS and apoptosis. Finally, we showed that CK inhibited the growth of liver cancer xenografts with little toxicity. Mice bearing human HCC xenografts that were treated with CK showed increased GRP78 expression and decreased p-STAT3 levels. Taken together, these data showed that CK induced ERS and apoptosis by inhibiting p-STAT3 in human liver cancer cells; thus, CK might be a potential therapeutic candidate for human HCC. [ABSTRACT FROM AUTHOR]- Published
- 2018
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9. Curcumin induces mitochondrial apoptosis in human hepatoma cells through BCLAF1-mediated modulation of PI3K/AKT/GSK-3β signaling.
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Bai, Chunhua, Zhao, Jiaqi, Su, Jielin, Chen, Jiaxin, Cui, Xinmu, Sun, Manqing, and Zhang, Xuewu
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CELL cycle , *CURCUMIN , *HEPATOCELLULAR carcinoma , *CHINESE medicine , *CELL anatomy , *MITOCHONDRIA - Abstract
Curcumin is a yellow pigment extracted from the rhizome of turmeric, a traditional Chinese medicine. Here, we tested the hypothesis that curcumin-mediated downregulation of BCLAF1 triggers mitochondrial apoptosis in hepatoma cells by inhibiting PI3K/AKT/GSK-3β signaling. Treatment of the human hepatoma cell lines, HepG2 and SK-Hep-1, with various concentrations of curcumin revealed a time-dependent and concentration-dependent inhibition of cell proliferation, increased apoptosis, cell cycle arrest at the G0/G1 phase, reduced mitochondrial membrane potential, and reduced expression levels of PI3K, p-PI3K, AKT, p-AKT, GSK-3β, and p-GSK-3β. Additionally, curcumin suppressed the levels of apoptotic factors after treating the cells with LY294002, a PI3K inhibitor. Curcumin also suppressed the expression of BCLAF1. Treating stable BCLAF1 knockout HepG2 and SK-Hep-1 cells with curcumin further enhanced apoptosis and increased the number of cells in G0/G1 cell cycle arrest, while inhibiting the downregulation of PI3K/AKT/GSK-3β pathway-related proteins. Treatment of a nude mouse xenograft model bearing HepG2 cells with curcumin inhibited tumor growth, disrupted the cellular structure of the tumor tissue, and suppressed the expression of BCLAF1 and PI3K/AKT/GSK-3β proteins. In summary, our in vitro and in vivo analyses show that curcumin downregulates BCLAF1 expression, inhibits the activation of the PI3K/AKT/GSK-3β pathway, and triggers mitochondrial apoptosis in HCC. These findings uncover a potential therapeutic strategy leveraging the antitumor effects of curcumin against HCC. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2022
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