1. Rapid determination of serum amyloid A using an upconversion luminescent lateral flow immunochromatographic strip.
- Author
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Sun X, Dai X, Ling S, Dong W, Chen D, Li M, Qiao X, Wang Z, Fan X, and Qian G
- Subjects
- Serum Amyloid A Protein, Silicon Dioxide, Prognosis, Luminescence, Nanoparticles chemistry
- Abstract
The early diagnosis and real-time prognosis of cardiovascular diseases (CVDs) at the bedside are important. However, real-time detection of myocardial infarction involves the use of large-scale instrumentation and long test times. Herein, a simple, rapid and sensitive lateral flow immunochromatographic strip (LFIS) based on Yb/Er co-doped NaYF
4 upconversion nanoparticles (UCNPs) was demonstrated for use in the detection of myocardial infarction. First, through heavy Yb/Er doping and an inert NaYF4 shell coating on the nanoparticles, the surface-related luminescence quenching effect of UCNPs was eliminated to enhance the upconversion luminescence. Second, through uniform coating of a SiO2 layer on the UCNPs, the biological affinity was improved to couple UCNPs and antibody proteins. Finally, through modification and activation with a specific antibody protein (serum amyloid A (SAA)), the UCNPs exhibited intense upconversion luminescence and high specificity when applied as a lateral flow immunochromatographic strip (LFIS). The developed UC-LFIS was highly sensitive (0.1 μg mL-1 ) and specific for detecting SAA in only 10 μL of serum. The UC-LFIS holds great potential for the early diagnosis and prognosis of CVDs.- Published
- 2023
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