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3 results on '"Di Teodoro, G."'

1. Respiratory explants as a model to investigate early events of contagious bovine pleuropneumonia infection.

Author

Di Teodoro G, Marruchella G, Di Provvido A, Orsini G, Ronchi GF, D'Angelo AR, D'Alterio N, Sacchini F, and Scacchia M

Subjects
Animals, Cattle, Disease Models, Animal, Fluorescent Antibody Technique, Indirect veterinary, Immunohistochemistry veterinary, Microscopy, Confocal veterinary, Bronchi microbiology, Cattle Diseases microbiology, Lung microbiology, Mycoplasma mycoides physiology, Pleuropneumonia, Contagious microbiology, Trachea microbiology
Abstract

Contagious bovine pleuropneumonia (CBPP) is a severe disease caused by Mycoplasma mycoides subsp. mycoides (Mmm). Knowledge on CBPP pathogenesis is fragmented and hampered by the limited availability of laboratory animal and in vitro models of investigation. The purpose of the present study is to assess respiratory explants as useful tools to study the early stages of CBPP. Explants were obtained from trachea, bronchi and lungs of slaughtered cattle, tested negative for Mycoplasma spp. and for the major bacterial and viral respiratory pathogens. The interaction of Mmm with explant cells was studied by immunohistochemistry (IHC), double-labelling indirect immunofluorescence (DLIIF) and laser scanning confocal microscopy (LSCM). Mmm capability to survive and proliferate within the explants was evaluated by standard microbiological procedures. Finally, the putative cellular internalization of Mmm was further investigated by the gentamicin invasion assay. IHC and DLIIF indicated that Mmm can colonize explants, showing a marked tropism for lower airways. Specifically, Mmm was detected on/inside the bronchiolar and alveolar epithelial cells, the alveolar macrophages and the endothelial cells. The interaction between Mmm and explant cells was abolished by the pre-incubation of the pathogen with bovine anti-Mmm immune sera. Mmm was able to survive and proliferate in all tracheal, bronchial and lung explants, during the entire time course of the experiments. LSCM and gentamicin invasion assay both confirmed that Mmm can enter non-phagocytic host cells. Taken together, our data supports bovine respiratory explants as a promising tool to investigate CBPP, alternative to cattle experimental infection.

Published
2018
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2. Lung lesion score system in cattle: proposal for contagious bovine pleuropneumonia.

Author

Di Provvido A, Di Teodoro G, Muuka G, Marruchella G, and Scacchia M

Subjects
Animals, Cattle, Mycoplasma mycoides, Lung pathology, Pleuropneumonia, Contagious pathology
Abstract

Contagious bovine pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides. The peculiar pathological features of CBPP make desirable the assessment of ad hoc score methods to grade the disease in the affected animals. Thus, the present work aims to assess a new lung score system for CBPP. Our results indicate that the present score system strongly correlates with that previously published by Turner and could be effectively used in CBPP-affected animals.

Published
2018
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3. Epidemiology, pathological aspects and genome heterogeneity of feline morbillivirus in Italy

Author

Antonella Tinelli, Shadia Berjaoui, Giovanni Di Teodoro, Francesco Dondi, Eliana De Luca, Alessio Lorusso, Francesca Cito, Giovanni Savini, Andrea Boari, Paolo Emidio Crisi, Daria Di Sabatino, Daniela Malatesta, Maria Loredana Colaianni, Paola Ripà, Nicola D'Alterio, Maurilia Marcacci, Ilaria Puglia, Giacomo Vincifori, De Luca E., Crisi P.E., Marcacci M., Malatesta D., Di Sabatino D., Cito F., D'Alterio N., Puglia I., Berjaoui S., Colaianni M.L., Tinelli A., Ripa P., Vincifori G., Di Teodoro G., Dondi F., Savini G., Boari A., and Lorusso A.

Subjects
Pathology, medicine.medical_specialty, Genotype, Epidemiology, Feline morbilliviru, Genome, Viral, Cat Diseases, Kidney, Microbiology, Virus, Article, 03 medical and health sciences, Genetic Heterogeneity, Morbillivirus, medicine, Prevalence, Tubulointerstitial nephritis, Animals, Viral, Tubulointerstitial nephriti, Lung, Tropism, Phylogeny, 030304 developmental biology, Feline morbillivirus, 0303 health sciences, CATS, Genome, General Veterinary, biology, 030306 microbiology, Genetic heterogeneity, Virus histochemistry, Brain, Cats, Italy, Morbillivirus Infections, RNA, Viral, Viral Tropism, General Medicine, biology.organism_classification, medicine.disease, medicine.anatomical_structure, Tissue tropism, RNA, Kidney disease
Abstract

Highlights • Prevalence of FeMV in feline colonies was higher with respect household cats. • FeMVs of this study belong to the genotype 1 and segregate into two clusters. • Isolation has been confirmed to be difficult and time consuming. • No statistically significant correlation was found between FeMV infection and TIN. • Virus histochemistry revealed immunoreactivity in lungs, kidneys and brain sections., Feline morbillivirus (FeMV) is an emerging morbillivirus first described in cats less than a decade ago. FeMV has been associated with chronic kidney disease of cats characterized by tubulointerstitial nephritis (TIN), although this aspect is still controversial and not demonstrated with certainty. To investigate FeMV prevalence and genomic characteristics, an epidemiological survey was conducted in a total number of 127 household cats originating from two Italian regions, Abruzzi and Emilia-Romagna. A total number of 69 cats originating from three feline colonies were also enrolled for the study. Correlation with TIN was investigated by employing a total number of 35 carcasses. Prevalence of FeMV RNA was higher in urine samples collected from cats of colonies (P = 31.8%, CI 95% 22.1–43.6) compared to household cats (P = 8.66%, CI 95% 4.9–14.9) and in young and middle-aged cats while prevalence of FeMV Abs was higher in old cats. Sequences obtained straight from infected biological samples, either partial or complete, cluster into two clades within FeMV genotype 1, distantly related to FeMV genotype 2. Immunohistochemistry analysis of kidney sections of FeMV RNA positive cats revealed immunoreactivity within epithelial cells of renal tubuli and inflammatory cells. However, statistically significant association between FeMV and renal damages, including TIN, was not demonstrated (p= 0.0695, Fisher exact test). By virus histochemistry performed with FeMV-negative feline tissues and a FeMV isolate, tropism for different cellular types such as inflammatory cells residing in blood vessels of kidney and brain, airway epithelial cells, alveolar macrophages and to a lesser extent, the central nervous system, was demonstrated. Additional studies are warranted in order to establish viral tropism and immune response during the early phases of infection and to disentangle the role of FeMV in co-infection processes.

Published
2019

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