3 results on '"Lindberg, Amanda"'
Search Results
2. Infiltration of NK and plasma cells is associated with a distinct immune subset in non‐small cell lung cancer.
- Author
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Backman, Max, La Fleur, Linnéa, Kurppa, Pinja, Djureinovic, Dijana, Elfving, Hedvig, Brunnström, Hans, Mattsson, Johanna Sofia Margareta, Lindberg, Amanda, Pontén, Victor, Eltahir, Mohamed, Mangsbo, Sara, Gulyas, Miklos, Isaksson, Johan, Jirström, Karin, Kärre, Klas, Leandersson, Karin, Mezheyeuski, Artur, Pontén, Fredrik, Strell, Carina, and Lindskog, Cecilia
- Subjects
NON-small-cell lung carcinoma ,KILLER cells ,PLASMA cells ,GENETIC mutation ,GENETIC load - Abstract
Immune cells of the tumor microenvironment are central but erratic targets for immunotherapy. The aim of this study was to characterize novel patterns of immune cell infiltration in non‐small cell lung cancer (NSCLC) in relation to its molecular and clinicopathologic characteristics. Lymphocytes (CD3+, CD4+, CD8+, CD20+, FOXP3+, CD45RO+), macrophages (CD163+), plasma cells (CD138+), NK cells (NKp46+), PD1+, and PD‐L1+ were annotated on a tissue microarray including 357 NSCLC cases. Somatic mutations were analyzed by targeted sequencing for 82 genes and a tumor mutational load score was estimated. Transcriptomic immune patterns were established in 197 patients based on RNA sequencing data. The immune cell infiltration was variable and showed only poor association with specific mutations. The previously defined immune phenotypic patterns, desert, inflamed, and immune excluded, comprised 30, 13, and 57% of cases, respectively. Notably, mRNA immune activation and high estimated tumor mutational load were unique only for the inflamed pattern. However, in the unsupervised cluster analysis, including all immune cell markers, these conceptual patterns were only weakly reproduced. Instead, four immune classes were identified: (1) high immune cell infiltration, (2) high immune cell infiltration with abundance of CD20+ B cells, (3) low immune cell infiltration, and (4) a phenotype with an imprint of plasma cells and NK cells. This latter class was linked to better survival despite exhibiting low expression of immune response‐related genes (e.g. CXCL9, GZMB, INFG, CTLA4). This compartment‐specific immune cell analysis in the context of the molecular and clinical background of NSCLC reveals two previously unrecognized immune classes. A refined immune classification, including traits of the humoral and innate immune response, is important to define the immunogenic potency of NSCLC in the era of immunotherapy. © 2021 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
3. Spatial immunophenotyping of the tumour microenvironment in non–small cell lung cancer.
- Author
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Backman, Max, Strell, Carina, Lindberg, Amanda, Mattsson, Johanna S.M., Elfving, Hedvig, Brunnström, Hans, O'Reilly, Aine, Bosic, Martina, Gulyas, Miklos, Isaksson, Johan, Botling, Johan, Kärre, Klas, Jirström, Karin, Lamberg, Kristina, Pontén, Fredrik, Leandersson, Karin, Mezheyeuski, Artur, and Micke, Patrick
- Subjects
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LUNG cancer , *DENDRITIC cells , *B cells , *CARCINOGENESIS , *MULTIVARIATE analysis , *KILLER cells , *MACROPHAGES , *REGRESSION analysis , *IMMUNOPHENOTYPING , *DESCRIPTIVE statistics , *T cells , *MYELOID cells , *PROPORTIONAL hazards models - Abstract
Immune cells in the tumour microenvironment are associated with prognosis and response to therapy. We aimed to comprehensively characterise the spatial immune phenotypes in the mutational and clinicopathological background of non–small cell lung cancer (NSCLC). We established a multiplexed fluorescence imaging pipeline to spatially quantify 13 immune cell subsets in 359 NSCLC cases: CD4 effector cells (CD4-Eff), CD4 regulatory cells (CD4-Treg), CD8 effector cells (CD8-Eff), CD8 regulatory cells (CD8-Treg), B-cells, natural killer cells, natural killer T-cells, M1 macrophages (M1), CD163+ myeloid cells (CD163), M2 macrophages (M2), immature dendritic cells (iDCs), mature dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs). CD4-Eff cells, CD8-Eff cells and M1 macrophages were the most abundant immune cells invading the tumour cell compartment and indicated a patient group with a favourable prognosis in the cluster analysis. Likewise, single densities of lymphocytic subsets (CD4-Eff, CD4-Treg, CD8-Treg, B-cells and pDCs) were independently associated with longer survival. However, when these immune cells were located close to CD8-Treg cells, the favourable impact was attenuated. In the multivariable Cox regression model, including cell densities and distances, the densities of M1 and CD163 cells and distances between cells (CD8-Treg–B-cells, CD8-Eff–cancer cells and B-cells–CD4-Treg) demonstrated positive prognostic impact, whereas short M2–M1 distances were prognostically unfavourable. We present a unique spatial profile of the in situ immune cell landscape in NSCLC as a publicly available data set. Cell densities and cell distances contribute independently to prognostic information on clinical outcomes, suggesting that spatial information is crucial for diagnostic use. • Immune cells showed specific infiltration patterns within the tumour microenvironment. • Infiltration of lymphocytes and plasmacytoid dendritic cells are associated with good prognosis. • Distances of immune cells to each other or to tumour cells reveal an independent prognostic impact. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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