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Powder probiotic has a good effect on the digestive tract which improvement of the immune system. This study to know the impact of powder probiotics on the immune system which includes levels of leukocytes, heterophils, lymphocytes, and heterophils to lymphocytes ratio in laying hens age 90 weeks. This study was conducted from February to March 2021 at Laying Hens Farm in Sukarapih Village, Sukasari, Sumedang, Jawa Barat. The object study were 40 laying hens aged 90 weeks. Completely Randomized Design (CRD) was applied which consists of four treatments and five replications. The treatments are basal ration without powder probiotic (T0); basal ration + 2 % powder probiotic (T1); basal ration + 3% powder probiotic (T2); and basal ration + 4% powder probiotic (T3). Statically this study showed no significant difference in levels of leukocyte, heterophil, lymphocyte, and heterophil to lymphocyte ratio. However, the administration of powder probiotic 4 % has improved the levels of, heterophil, lymphocyte, and heterophil to lymphocyte ratio on laying hens aged 90 weeks near rejected. [ABSTRACT FROM AUTHOR]