1. MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias.
- Author
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Calin, George Adrian, Chang-Gong Liu, Sevignani, Cinzia, Ferracin, Manuela, Felli, Nadia, Dumitru, Calin Dan, Shimizu, Masayoshi, Cimmino, Amelia, Zupo, Simona, Dono, Mariella, Dell'Aquila, Marie L., Alder, Hansjuerg, Rassenti, Laura, Kipps, Thomas J., Bullrich, Florencia, Negrini, Massimo, and Croce, Carlo M.
- Subjects
LYMPHOCYTIC leukemia ,CHRONIC diseases ,CHRONIC lymphocytic leukemia ,LYMPHOPROLIFERATIVE disorders ,LEUKEMIA ,LYMPHOCYTES ,GENE expression - Abstract
Little is known about the expression levels or function of microRNAs (miRNAs) in normal and neoplastic cells, although it is becoming clear that miRNAs play important roles in the regulation of gene expression during development [Ambros, V. (2003) Cell 113, 673-676; McManus, M. T. (2003) Semin. Cancer Blot 13, 253-258]. We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. This approach allowed us to identify significant differences in miRNome expression between CLI samples and normal CD5+ B cells; data were confirmed by Northern blot analyses and real-time RT-PCR. At least two distinct clusters of CLI samples can be identified that were associated with the presence or absence of Zap-b expression, a predictor of early disease progression. Two miRNA signatures were associated with the presence or absence of mutations in the expressed Ig variable-region genes or with deletions at 13q14, respectively. These data suggest that miRNA expression patterns have relevance to the biological and clinical behavior of this leukemia. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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