1. Mechanism of Conversion of the Salmonella O Antigen by Bacteriophage ε34
- Author
-
Andrew Wright
- Subjects
Electrophoresis ,Lipopolysaccharides ,Paper ,Double bond ,Chemical Phenomena ,Rhamnose ,Stereochemistry ,Chromatography, Paper ,Genetics and Molecular Biology ,Tritium ,Microbiology ,Chromatography, DEAE-Cellulose ,Bacteriophage ,Electron Transport ,chemistry.chemical_compound ,Antigen ,Salmonella ,Lysogenic cycle ,Antigens ,Molecular Biology ,Lysogeny ,chemistry.chemical_classification ,Carbon Isotopes ,biology ,Spectrum Analysis ,Phosphorus Isotopes ,Nucleosides ,biology.organism_classification ,carbohydrates (lipids) ,Paper chromatography ,Chemistry ,Glucose ,chemistry ,Biochemistry ,Phosphodiester bond ,Mutation ,Uridine diphosphate glucose ,lipids (amino acids, peptides, and proteins) ,Salmonella Phages - Abstract
The structural determinants for antigen 34 in the E group salmonella are glucosyl substituents on the galactosyl units of the O antigen which has a mannosylrhamnosylgalactose repeating sequence. The temperate bacteriophage ε 34 brings about the production of antigen 34. It has been shown here that glucose is transferred from uridine diphosphate glucose to the O antigen via a glucosyl-lipid intermediate in a two-step reaction. Glucose is linked through carbon 1 to the lipid by a phosphodiester bridge, the glucosyl bond having the β-anomeric configuration. The lipid is a C 55 -polyisoprenoid alcohol, each isoprene unit having one double bond. It is the same lipid which is involved in the synthesis of the O antigen repeating sequence.
- Published
- 1971