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Start Over Author "Secco, Chiara" Topic macrophages
2 results on '"Secco, Chiara"'

1. Frontline Science: Mast cells regulate neutrophil homeostasis by influencing macrophage clearance activity.

Author

Jachetti E, D'Incà F, Danelli L, Magris R, Dal Secco C, Vit F, Cancila V, Tripodo C, Scapini P, Colombo MP, Pucillo C, and Frossi B

Subjects
Animals, Bone Marrow Cells cytology, CD11b Antigen metabolism, Cell Count, Cytokines metabolism, Granulocyte Colony-Stimulating Factor metabolism, Hematopoiesis, Inflammation Mediators metabolism, Interleukin-17 metabolism, Mice, Inbred C57BL, Myeloid Cells metabolism, Phenotype, Proto-Oncogene Proteins c-kit metabolism, Signal Transduction, Homeostasis, Macrophages metabolism, Mast Cells metabolism, Neutrophils metabolism
Abstract

The receptor tyrosine kinase cKit and its ligand stem cell factor are essential for mast cells (MC) development and survival. Strains with mutations affecting the Kit gene display a profound MC deficiency in all tissues and have been extensively used to investigate the role of MC in both physiologic and pathologic conditions. However, these mice present a variety of abnormalities in other immune cell populations that can affect the interpretation of MC-related responses. C57BL/6 Kit W-sh are characterized by an aberrant extramedullary myelopoiesis and systemic neutrophilia. MC deficiency in Kit W-sh mice can be selectively repaired by engraftment with in vitro-differentiated MC to validate MC-specific functions. Nevertheless, the impact of MC reconstitution on other immune populations has never been evaluated in detail. Here, we specifically investigated the neutrophil compartment in primary and secondary lymphoid organs of C57BL/6 Kit W-sh mice before and after MC reconstitution. We found that, albeit not apparently affecting neutrophils phenotype or maturation, MC reconstitution of Kit W-sh mice restored the number of neutrophils at a level similar to that of wild-type C57BL/6 mice. In vitro and ex vivo experiments indicated that MC can influence neutrophil clearance by increasing macrophages' phagocytic activity. Furthermore, the G-CSF/IL-17 axis was also influenced by the presence or absence of MC in Kit W-sh mice. These data suggest that MC play a role in the control of neutrophil homeostasis and that this aspect should be taken into account in the interpretation of results obtained using Kit W-sh mice., (©2019 Society for Leukocyte Biology.)

Published
2019
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2. Frontline Science: Mast cells regulate neutrophil homeostasis by influencing macrophage clearance activity

Author

Barbara Frossi, Federica D’Incà, Carlo Pucillo, Raffaella Magris, Mario P. Colombo, Luca Danelli, Filippo Vit, Valeria Cancila, Claudio Tripodo, Chiara Dal Secco, Patrizia Scapini, Elena Jachetti, Jachetti, Elena, D'Incà, Federica, Danelli, Luca, Magris, Raffaella, Dal Secco, Chiara, Vit, Filippo, Cancila, Valeria, Tripodo, Claudio, Scapini, Patrizia, Colombo, Mario Paolo, Pucillo, Carlo, and Frossi, Barbara

Subjects
0301 basic medicine, Immunology, Kit (W-sh) mice, macrophages, mast cell, neutrophils, phagocytosis, Bone Marrow Cells, Cell Count, Stem cell factor, macrophage, Receptor tyrosine kinase, 03 medical and health sciences, 0302 clinical medicine, Immune system, Granulocyte Colony-Stimulating Factor, medicine, Animals, Homeostasis, Immunology and Allergy, Macrophage, Myeloid Cells, Mast Cells, Neutrophil homeostasis, CD11b Antigen, Neutrophil clearance, biology, Interleukin-17, neutrophil, Cell Biology, Neutrophilia, Hematopoiesis, Cell biology, Mice, Inbred C57BL, Proto-Oncogene Proteins c-kit, Phenotype, 030104 developmental biology, biology.protein, Cytokines, Inflammation Mediators, medicine.symptom, Ex vivo, Signal Transduction, 030215 immunology
Abstract

The receptor tyrosine kinase cKit and its ligand stem cell factor are essential for mast cells (MC) development and survival. Strains with mutations affecting the Kit gene display a profound MC deficiency in all tissues and have been extensively used to investigate the role of MC in both physiologic and pathologic conditions. However, these mice present a variety of abnormalities in other immune cell populations that can affect the interpretation of MC-related responses. C57BL/6 KitW-sh are characterized by an aberrant extramedullary myelopoiesis and systemic neutrophilia. MC deficiency in KitW-sh mice can be selectively repaired by engraftment with in vitro-differentiated MC to validate MC-specific functions. Nevertheless, the impact of MC reconstitution on other immune populations has never been evaluated in detail. Here, we specifically investigated the neutrophil compartment in primary and secondary lymphoid organs of C57BL/6 KitW-sh mice before and after MC reconstitution. We found that, albeit not apparently affecting neutrophils phenotype or maturation, MC reconstitution of KitW-sh mice restored the number of neutrophils at a level similar to that of wild-type C57BL/6 mice. In vitro and ex vivo experiments indicated that MC can influence neutrophil clearance by increasing macrophages’ phagocytic activity. Furthermore, the G-CSF/IL-17 axis was also influenced by the presence or absence of MC in KitW-sh mice. These data suggest that MC play a role in the control of neutrophil homeostasis and that this aspect should be taken into account in the interpretation of results obtained using KitW-sh mice.

Published
2019
Full Text
View/download PDF

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