1. HIV-1 susceptibility of transgenic rat-derived primary macrophage/T cells and a T cell line that express human receptors, CyclinT1 and CRM1 genes.
- Author
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Shida H, Okada H, Suzuki H, Zhang X, Chen J, Tsunetsugu-Yokota Y, Tanaka Y, Yakushiji F, and Hayashi Y
- Subjects
- Animals, CD4-Positive T-Lymphocytes virology, Cell Line, Cells, Cultured, Cyclin T genetics, Disease Susceptibility, HIV-1 pathogenicity, Humans, Karyopherins genetics, Macrophages virology, Rats, Receptors, CXCR4 genetics, Receptors, CXCR4 metabolism, Receptors, Cytoplasmic and Nuclear genetics, Exportin 1 Protein, CD4-Positive T-Lymphocytes immunology, Cyclin T metabolism, HIV Infections immunology, Karyopherins metabolism, Macrophages immunology, Receptors, Cytoplasmic and Nuclear metabolism
- Abstract
We developed transgenic (Tg) rats that express human CD4, CCR5, CXCR4, CyclinT1, and CRM1 genes. Tg rat macrophages were efficiently infected with HIV-1 and supported production of infectious progeny virus. By contrast, both rat primary CD4
+ T cells and established T cell lines expressing human CD4, CCR5, CyclinT1, and CRM1 genes were infected inefficiently, but this was ameliorated by inhibition of cyclophilin A. The infectivity of rat T cell-derived virus was lower than that of human T cell-derived virus., (© 2017 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.)- Published
- 2017
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