1. Involvement of pattern recognition receptors in the induction of cytokines and reactive oxygen intermediates production by human monocytes/macrophages stimulated with tumour cells.
- Author
-
Mytar B, Woloszyn M, Macura-Biegun A, Hajto B, Ruggiero I, Piekarska B, and Zembala M
- Subjects
- Adenocarcinoma metabolism, Adenocarcinoma pathology, Antibodies, Monoclonal pharmacology, CD36 Antigens immunology, Cell Communication physiology, Cell Line, Tumor, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, Humans, Lectins, C-Type immunology, Lectins, C-Type metabolism, Ligands, Lipoproteins, LDL metabolism, Lipoproteins, LDL pharmacology, Macrophages cytology, Macrophages immunology, Mannose Receptor, Mannose-Binding Lectins immunology, Mannose-Binding Lectins metabolism, Monocytes cytology, Monocytes immunology, Pancreatic Neoplasms metabolism, Pancreatic Neoplasms pathology, Phosphatidylserines metabolism, Phosphatidylserines pharmacology, Receptors, Cell Surface immunology, Receptors, Cell Surface metabolism, Receptors, Immunologic immunology, Receptors, Immunologic metabolism, Receptors, Scavenger, Scavenger Receptors, Class A, Scavenger Receptors, Class B, Signal Transduction, Cytokines biosynthesis, Lectins, C-Type physiology, Macrophages metabolism, Mannose-Binding Lectins physiology, Monocytes metabolism, Reactive Oxygen Species metabolism, Receptors, Cell Surface physiology, Receptors, Immunologic physiology
- Abstract
Background: Some ligands of pattern recognition receptors (PRR) are present on tumour cells. The role of PRR in signalling for cytokine and reactive oxygen intermediates (ROI) production by monocytes and monocyte-derived macrophages (MDM) stimulated with tumour cells was studied., Materials and Methods: Monocytes/MDM were pretreated with PRR ligands or anti-PRR monoclonal antibodies (mAbs) and stimulated with tumour cells. Cytokine secretion was measured by enzyme-linked immunoassay (ELISA) and ROI production by luminol-dependent chemiluminescence (CL)., Results: The ligands of scavenger receptor A (SR-A): (fucoidan, polyguanylic acid (polyG) and modified low density lipoproteins (LDL)) and B (SR-B) (native and modified LDL, phosphatidylserine (PdS)) and of mannose receptor (MR) (mannan), induced tumour necrosis factor alpha (TNF) and ROI (except LDL) release by monocytes. Production of TNF and interleukin-10 (IL-10) by MDM was stimulated by SR-A ligands and mannan. Tumour cell-induced TNF and IL-10 production by monocytes, but not MDM, was diminished by fucoidan and polyG, while ROI release was reduced by MR and SR-A ligands. Supplementation of tumour cells with modified LDL and PdS enhanced their stimulatory capacity. TNF and ROI release by tumour cells-stimulated monocytes was inhibited by anti-CD36 and anti-MR (clone PAM-1) mAbs., Conclusion: SR and MR may be involved to different extents in the induction of cytokines and ROI production by monocytes, but not MDM, stimulated with tumour cells.
- Published
- 2004