1. Mitochondrial bioenergy alterations in avian HD11 macrophages infected with infectious bronchitis virus.
- Author
-
da Silva SEL, Ferreira HL, Garcia AF, Silva FES, Gameiro R, Fabri CUF, Vieira DS, and Cardoso TC
- Subjects
- Animals, Apoptosis, Cell Line, Cell Proliferation, Chickens, Infectious bronchitis virus growth & development, Macrophages metabolism, Membrane Potential, Mitochondrial, Mitochondria metabolism, Reactive Oxygen Species metabolism, Virion growth & development, Virus Replication, Host-Pathogen Interactions, Infectious bronchitis virus pathogenicity, Macrophages virology, Mitochondria virology, Virion pathogenicity
- Abstract
To establish an association between mitochondrial dysfunction and apoptosis following infectious bronchitis virus (IBV) infection, HD11 avian macrophage cells were infected with the Massachusetts 41 (M41) strain. Our results show that the M41 strain of IBV induced cytopathic effects followed by the release of new viral particles. Elevated numbers of apoptotic cells were observed at 24, 48 and 72 h post-infection (p.i.). Viral infection was associated with mitochondrial membrane depolarization and reactive oxygen species (ROS) production at all of the examined timepoints p.i. In summary, IBV M41 replication in infected HD11 macrophages seems to induce mitochondrial bioenergy failure, acting as a respiratory chain uncoupler, without compromising viral replication.
- Published
- 2018
- Full Text
- View/download PDF