1. Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor
- Author
-
Juliane Wunderlich, Sarah J. Reiling, John P. Dalton, Corine G. Demanga, Jenny W.L. Eng, and Petra Rohrbach
- Subjects
Plasmodium falciparum ,030231 tropical medicine ,Cell Culture Techniques ,Mycology & Parasitology ,Plasmodium ,Microbiology ,03 medical and health sciences ,Bioreactors ,0302 clinical medicine ,parasitic diseases ,Bioreactor ,medicine ,Humans ,Parasite hosting ,Malaria, Falciparum ,030304 developmental biology ,0303 health sciences ,biology ,biology.organism_classification ,medicine.disease ,3. Good health ,Infectious Diseases ,Wave bioreactor ,Parasitology ,Malaria - Abstract
We describe methods for the large-scale in vitro culturing of synchronous and asynchronous blood-stage Plasmodium falciparum parasites in sterile disposable plastic bioreactors controlled by wave-induced motion (wave bioreactor). These cultures perform better than static flask cultures in terms of preserving parasite cell cycle synchronicity and reducing the number of multiple-infected erythrocytes. The straight-forward methods described here will facilitate the large scale production of malaria parasites for antigen and organelle isolation and characterisation, for the high throughput screening of compound libraries with whole cells or extracts, and the development of live- or whole-cell malaria vaccines under good manufacturing practice compliant standards. © 2012.
- Published
- 2012