1. Induction of ssDNA-binding autoantibody secreting B cell immunity during murine malaria infection is a critical part of the protective immune responses.
- Author
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Mannoor K, Li C, Inafuku M, Taniguchi T, Abo T, Sato Y, and Watanabe H
- Subjects
- Adoptive Transfer, Animals, B-Lymphocytes parasitology, Cells, Cultured, Disease Models, Animal, Humans, Immunity, Active drug effects, Immunity, Humoral drug effects, Lymphocyte Depletion, Malaria therapy, Male, Mice, Mice, Inbred C57BL, Nanoparticles administration & dosage, Antibodies, Antinuclear immunology, B-Lymphocytes immunology, DNA, Single-Stranded immunology, Malaria immunology, Plasmodium yoelii immunology, Spleen immunology
- Abstract
Although it has been hypothesized that autoimmune-like phenomena may play a critical role in the protective immune responses to both human and animal malaria, there are still no evidence-based data to support this view. In this study we demonstrate that the majority of anti-single stranded (ss) DNA autoantibody secreting B cells were confined to B220(+)CD21(+)CD23(-) cells and that these cells expanded significantly in the spleen of C57BL/6 mice infected with Plasmodium yoelii 17X non-lethal (PyNL). To determine the role of ssDNA-binding autoantibody secreting B cell responses in murine malaria, we conjugated generation 6 (poly) amidoamine dendrimer nanoparticles with ssDNA to deplete ssDNA-binding autoreactive B cells in vivo. Our data revealed that 55.5% of mice died after DNA-coated nanoparticle-mediated in vivo depletion of ssDNA-specific autoreactive B cells and subsequent challenge using PyNL. Adoptive transfer of B cells with ssDNA specificity to mice, followed by PyNL infection, caused a later appearance and inhibition of parasitemia. The possible mechanism by which the ssDNA-binding autoantibody secreting B cells is involved in the protection against murine malaria has also been demonstrated., (Copyright © 2012 Elsevier GmbH. All rights reserved.)
- Published
- 2013
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