1. Protective effect of infliximab on ischemia/reperfusion-induced damage in rat kidney.
- Author
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Tasdemir C, Tasdemir S, Vardi N, Ates B, Parlakpinar H, Kati B, Karaaslan MG, and Acet A
- Subjects
- Acute Kidney Injury etiology, Acute Kidney Injury metabolism, Animals, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Disease Models, Animal, Infliximab, Kidney blood supply, Kidney Tubules drug effects, Kidney Tubules pathology, Male, Rats, Rats, Wistar, Reperfusion Injury metabolism, Reperfusion Injury pathology, Tumor Necrosis Factor-alpha antagonists & inhibitors, Acute Kidney Injury prevention & control, Antibodies, Monoclonal therapeutic use, Malondialdehyde metabolism, Peroxidase metabolism, Reperfusion Injury complications
- Abstract
Objective: To investigate the protective effect of infliximab on ischemia-reperfusion (I/R) injury of the rat kidney., Methods: Twenty-eight male Wistar albino rats were divided into four groups: sham-operated, I/R, I/R with infliximab administered before ischemia [I/R + infliximab (bi)], and I/R with infliximab administered before reperfusion [I/R + infliximab (br)]. After a right nephrectomy to produce damage, the left renal vessels were occluded for 60 min, followed by 24-h reperfusion in rats. Changes in the rat kidney were observed by measuring the tissue levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD) and by evaluating hematoxylin-eosin (H&E)-stained and periodic acid-Schiff (PAS) sections., Results: The MDA and MPO levels in the I/R group were significantly higher than in the other groups (p < 0.05), and the SOD and GSH levels in the I/R + infliximab (bi) and I/R + infliximab (br) groups were significantly higher than in the I/R group (p < 0.05). However, histological examination revealed that the I/R + infliximab (bi) group and the I/R + infliximab (br) group had significantly fewer tubular changes and interstitial inflammatory cell infiltration than the I/R group., Conclusion: These results show that infliximab may protect against I/R injury in the rat I/R model.
- Published
- 2012
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