1. Calcium binding to mastocyte plasma membrane.
- Author
-
Tolone G, Bonasera L, Vitale F, and Pontieri GM
- Subjects
- Animals, Binding Sites, Cell Membrane immunology, Hydrolysis, Membrane Proteins, Neuraminic Acids, Phospholipids, Pronase pharmacology, Rats, Trypsin pharmacology, Calcium metabolism, Mast Cells immunology
- Abstract
Rat mastocyte plasma membrane possess two major classes of calcium binding sites which have widely different affinity. At pH 7.5 the low affinity sites can bind about 88 nmol of Ca2+ per milligram of membrane protein and are half-saturated at 125 micro M Ca2+, whereas the high-affinity sites bind about 12 nmol of Ca2+ per milligram of membrane protein and are half-saturated at 5.5 micro M Ca2+. Membrane phospholipids and neuraminic acid residues account for approximately 87% of calcium binding.
- Published
- 1980
- Full Text
- View/download PDF