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Your search keyword '"Abdullah Alarfaj"' showing total 3 results
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3 results on '"Abdullah Alarfaj"'

1. Novel green synthesis of silver nanoparticles mediated by Curcumae kwangsiensis for anti-lung cancer activities: a preclinical trial study

Author

Zhihong Liu, Zhuohong Zhang, Xiaomei Du, Ying Liu, Abdullah Alarfaj, Abdurahman Hirad, Siddique Akber Ansari, and Zhiguo Zhang

Subjects
silver nanoparticles, curcumae kwangsiensis folium, antioxidant, cytotoxicity, anti-human lung cancer, Medicine
Abstract

Introduction The present work describes the green synthesis and characterization and cytotoxicity, antioxidant, and anti-human lung cancer activities of silver nanoparticles containing Curcumae kwangsiensis folium leaf aqueous extract. Material and methods Ag nanoparticles were produced by mixing the AgNO3 solution with aqueous C. kwangsiensis folium leaf extract. Characterization of Ag nanoparticles was done by FE-SEM, FT-IR, TEM, and UV-Vis. FE-SEM and TEM images revealed an average diameter of 15-21 nm for the nanoparticles. MTT assay was used on common human lung cancer cell lines, i.e., lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14) cell lines, to survey the cytotoxicity and anti-human lung cancer effects of Ag nanoparticles. Results They had very low cell viability and high anti-human lung cancer activities dose-dependently against HLC-1, LC-2/ad, and PC-14 cell lines without any cytotoxicity towards the normal cell line (HUVEC). The IC50 values of Ag nanoparticles were 249, 187, and 152 µg/ml against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. The best results of cytotoxicity and anti-human lung cancer properties were seen at the concentration of 1000 µg/ml. Ag nanoparticles inhibited half of the DPPH molecules in the concentration of 135 µg/ml. Maybe significant anti-human lung cancer potentials of Ag nanoparticles synthesized by C. kwangsiensis folium leaf aqueous extract against common human lung cancer cell lines are linked to their antioxidant activities. Conclusions After confirming the above results in the clinical trial research, this formulation can be administered to treat human lung cancers in humans.

Published
2021
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2. Antimicrobial Surveillance for Bacterial Uropathogens in Ha’il, Saudi Arabia: A Five-Year Multicenter Retrospective Study

Author

Rawan Thallab, Khalid Alshaghdali, Abdulrahman S Bazaid, Sahar A Hammam, Abdu Aldarhami, Amir Saeed, Munif H Alshammary, Talal Alreshidi, Abdullah Alarfaj, Abdulkarim Alrashidi, and Abdulaziz Alrashidi

Subjects
0301 basic medicine, Imipenem, medicine.medical_specialty, medicine.drug_class, 030106 microbiology, Antibiotics, Saudi Arabia, Meropenem, Enterococcus faecalis, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Antibiotic resistance, Internal medicine, medicine, Pharmacology (medical), AMR, 030212 general & internal medicine, antimicrobial resistance, Original Research, Pharmacology, biology, business.industry, Sulfamethoxazole, uropathogens, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, Trimethoprim, Infectious Diseases, chemistry, Infection and Drug Resistance, Linezolid, surveillance, drug-resistant bacteria, business, medicine.drug
Abstract

Abdulrahman S Bazaid,1 Amir Saeed,1,2 Abdulaziz Alrashidi,1 Abdulkarim Alrashidi,1 Khalid Alshaghdali,1 Sahar A Hammam,3 Talal Alreshidi,3 Munif Alshammary,3 Abdullah Alarfaj,1 Rawan Thallab,4 Abdu Aldarhami5 1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Ha’il, Ha’il, Saudi Arabia; 2Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Medical Sciences & Technology, Khartoum, Sudan; 3Ha’il Maternity Hospital, Ha’il, Saudi Arabia; 4Department of Statistics, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia; 5Department of Medical Microbiology, Qunfudah Faculty of Medicine, Umm Al-Qura University, Al-Qunfudah, 28814, Saudi ArabiaCorrespondence: Abdulrahman S BazaidDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Ha’il, Ha’il, Saudi ArabiaTel +966-165358200Email ar.bazaid@uoh.edu.saObjective: Urinary tract infections (UTIs) are among the most common infections that require antibiotic intervention. Antibiotic surveillance programs are crucial to assess resistance patterns of microorganisms associated with UTIs and to tailor antibiotic therapy accordingly. Therefore, the aim of the current study is to investigate the prevalence of uropathogens and their antimicrobial susceptibility patterns in Ha’il region.Methods: We conducted a retrospective study in two main hospitals in Ha’il over a 5-year period (January 2015 to December 2019). Laboratory reports and clinical data of patients with a positive urine culture (≥ 105 CFU/mL) were included in the study.Results: A total of 428 patients were included in this study. The majority of positive cultures belonged to female patients (94.4%), adults (76.9%) and outpatients (74.3%). Generally, Escherichia coli was the most common pathogen (45%), followed by Klebsiella pneumoniae (17%) and Staphylococcus aureus (12%). Extended-spectrum β-lactamase (ESBL) strains of E. coli (15.7%) and K. pneumoniae (19.7%) showed resistance to most tested antibiotics, while almost all Gram-negative uropathogens including ESBL strains presented low resistance to cefoxitin, imipenem and meropenem. Most strains of Staphylococcus aureus and Enterococcus faecalis were resistant to trimethoprim/sulfamethoxazole including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VRE), whereas the activity of linezolid was monitored against almost all different strains of Gram-positive uropathogens.Conclusion: This surveillance study which was conducted over a 5-year period in the Ha’il region revealed that the most common UTI-associated pathogen was E. coli. Based on the current sensitivity profiles obtained from this surveillance, carbapenems and linezolid can be considered as a first therapeutic choice treating UTIs in Ha’il caused by Gram-negative and positive uropathogens, respectively. Frequent targeted surveillance programs for antibiotic-resistant pathogens and their susceptibility profiles are crucial to enable tailored empirical treatment for patients.Keywords: surveillance, antimicrobial resistance, AMR, uropathogens, Saudi Arabia, drug-resistant bacteria

Published
2021

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