Search

Seventy two (72) sexed crossbred rabbits aged 6 months were used to determine the effect of ginger rhizome powder (GRP) supplementation on reproductive organ and serum sex hormonal characteristics of pubertal rabbits. The experimental rabbits were divided into 2 treatment groups comprising 36 males and 36 females. Each group was divided into 4 dietary treatments (BT1, BT2, BT3 and BT4 ) of 9 animals and replicated thrice with 3 animals per replicate in a completely randomized design (CRD). The animals were fed formulated diet containing 2900 Kcal ME kg-1 and 18% crude protein supplemented with GRP at the rate of 0, 5, 10 and 15 g kg-1 feed respectively. Rabbits in each dietary group were fed 50gms of Guinea grass leaf meal containing 27.6% CF (dry matter basis) occasionally as additional fibre sources. Feed and water were offered ad libitum and the study lasted for 10 weeks. Testosterone values of BT (5.83 ng mL-1) and BT (6.28 ng mL-1) and follicle stimulating hormone (FSH) levels of BT (1.15 mIU mL-1) were significantly influenced (p

A 35-day feeding trial involving three hundred (300) five (5) week old broilers was carried out in a completely randomized design to evaluate the haematology, serum biochemical and gastro-intestinal characteristics of broiler finishers fed diets containing a mixture of fermented bovine blood and rumen digesta (FBBRD) meal at dietary levels of 0, 5, 10, 15 and 20 %, respectively. The birds were randomly grouped into five, with sixty birds each and four replicates of fifteen birds per treatment. At the end of the experiment, blood samples were collected from eight birds randomly selected from each treatment for haematological and biochemical analyses. The group on 10 % FBBRD diet had significantly (P

One of the reliable information for evaluating nutritional status, performance and physiological state of farm animals to nutrient utilization is through haematological indices assessment. Haematological parameters of grower pigs fed low crude protein and low energy diets supplemented with multi-enzyme (enziblend plus+) were determined using 36 hybrid (Landrace x Large white) male pigs of 8-10 weeks old. Two metabolizable energy (3000 and 2600 Kcal ME/kg) and three crude protein levels (18, 16 and 14 % CP) were used to formulate Six dietary treatments; T1 (3000 Kcal ME/Kg; 18 % CP), T2 (3000 Kcal ME/Kg; 16 % CP+ 1g of enzyme/kg diet), T3 (3000 Kcal ME/Kg; 14 % CP+ 1g of enzyme/kg diet), T4 (2600 Kcal ME/Kg; 18 % CP + 1g of enzyme/kg diet), T5 (2600 Kcal ME/Kg; 16 % CP + 1g of enzyme/kg diet) and T6 (2600 Kcal ME/Kg; 14 % CP + 1g of enzyme/kg diet). The treatments were replicated three with two pigs per replicate. The experiment was a 2 x 3 factorial, and T1 served as the control. Blood samples were collected (3mls) via the ear vein from one pig in each replicate, three from treatment for haematological analysis. The results of this study recorded significant differences (P

This study was conducted for 4 months in the quest to discover natural materials, especially of plant origin, capable of substituting the roles of synthetic materials, especially drugs, in reproductive enhancement of livestock species. Such natural materials will help eliminate the menace of residual effects of synthetic materials on livestock products and probably attract more revenue from them. A total of 24 New Zealand White (NZW) weaned rabbit bucks were used and the study was carried out in a Complete Randomized Design (CRD), comprising three treatments replicated four times; MT1 (control), MT2 and MT3 , containing 0, 200 and 400 mg/kg body weight of Panax ginseng extracts (PGEs), respectively. Assessment for attainment of puberty started from the age of 11 - 14 weeks on a weekly basis and at 72 h interval thereafter by examining their ejaculate and preputial fluid smeared on a glass slide under a microscope for sperm cells. Semen was collected twice a week for 3 weeks after maturity (6 months old) with the aid of an artificial vagina (AV), using rabbit does as teasers. Semen analysis was done within 20 minutes of collection, following standard procedures. Libido was determined weekly for 3 weeks by recording the time it took the matured rabbit bucks to sniff, groom, and mount the rabbit doe in the initial instance (reaction time); and by counting the number of times the matured buck attempts to mount the doe per minute (libido score). Data generated from this study was analyzed using analysis of variance (ANOVA). Age at puberty (MT1 = 116.00 days; MT2 = 112.50 days; MT3 = 100.50 days) was significantly (P

Introduction The pharynx is an important site with respect to transmission and development of antimicrobial resistance (AMR) to gonorrhoea particularly in men who have sex with men. Maintaining culture-based sensitivity testing is important as part of wider efforts to prevent transmission of high level AMR strains of gonorrhoea. The sensitivity of gonorrhoea culture at the pharynx is low and is dependent on several factors including sampling technique by clinicians. There is no consensus on the length of time taken to sample the pharynx for culture. We aimed to explore knowledge of pharyngeal sampling among sexual health clinicians to gain a consensus on optimal time for pharyngeal sampling for gonorrhoea culture specimens. Method An online anonymous survey was sent to clinicians in our local sexual health network. We included pictorial diagrams of the pharynx and asked clinicians which sites in the pharynx they sample and to estimate how long for when taking gonorrhoea culture specimens. Result 110 clinicians responded to the survey of whom 98 regularly take pharyngeal gonorrhoea cultures specimens: 54(55%) had more than 10-years of experience in sexual health. 82(84%) sample the tonsils, 75(77%) sample the posterior pharyngeal wall and 94(96%) sample either the tonsils or the posterior pharyngeal wall. 30(31%) always attempt to elicit the gag reflex and 52(53%) do not. The average time taken sampling the pharynx was 4.63(SD±2.04) seconds. There was no significant difference in time taken to sample the pharynx according to years of experience (4.7(SD±2.02)v4.6(SD±2.3),p=0.45). Conclusion Experienced sexual health clinicians are mostly appropriately sampling the tonsils and posterior pharyngeal wall for gonorrhoea culture specimens, and the median time taken to sample the pharynx was 4.63 seconds. Standardisation of pharyngeal sampling for the pharynx for gonorrhoea culture specimens could improve the sensitivity of culture and educational tools for clinic staff to support this are needed.

Treatment with Panax ginseng has been reported to elicit various biological effects in both animal and human models. Among the reported effects on animal are protein synthesis and growth promoting ability. For a better understanding, the current experiment was conducted to evaluate the effect of oral administration of P. ginseng extracts (PGEs) on growth performance parameters of growing New Zealand White (NZW) rabbits. A total of 48 NZW male and female rabbits, with the average age of two months were used for the experiment, conducted in a Randomized Complete Block Design (RCBD). The rabbits were organized into 3 groups of 16 rabbits each (8 males and 8 females), on weight equalization basis and randomly assigned to 3 treatments: T1, T2 and T3, containing PGEs at 0.0, 200.0 and 400.0 mg/ml, respectively. Final body weight and total body weight gain of the male rabbits on T2 and T3 were significantly (p < 0.05) higher than those of male rabbits on T1, while feed conversion ratio for the male rabbits recorded a higher (p < 0.05) value in T1 than in T2 and T3, which were similar (p < 0.05). Trunk length and breast girth of the male rabbits were significantly (p < 0.05) higher in T3 than in T1 and T2. Feed intake pattern was similar among the male rabbits, but different between males and females. Significant (p < 0.05) differences were not observed in all parameters measured among female rabbits, but all experimental rabbits followed the same pattern of body weight gain. Oral administration of Panax ginseng extracts at 200 and 400 mg/ml levels enhanced feed conversion ratio in male NZW rabbits, thereby increasing body weight gain, trunk length and breast girth, while feed intake patterns slightly differed between the male and female growing rabbits.

Objectives: To investigate the biochemical effects of oral doses of Piper guineense (P. guineense) leaf extract on female diabetics using experimental animals. Methods: The animals, albino wistar rats, were divided into six groups (n=7). Animals in group 1 received water and feed only. Animals in groups 2 to 6 were induced with diabetes using alloxan. Methanolic leave extracts of P. Guineense were administered to groups 2 to 4 in 40 mg/kg, 80 mg/kg and 100 mg/kg body weights representing low, medium and high doses respectively. Group 5 animals were treated with 10 mg/kg body weight of Glibenclamide (Antidiabetic drug) and group 6 animals were left untreated. All treatments were carried out orally and lasted for a period of 14 days. At the end of the 14 days, the animals were humanely sacrificed through cardiac puncture and the blood samples collected for the analyses of some liver and kidney function parameters using assay kits. Results: The results showed that the oral doses of methanolic leave extract of P. guineense had no negative alterations on the biochemical parameters analyzed namely, 1) Lipid profile (Triglyceride, Low Density Lipoprotein, Total Cholesterol and High Density Lipoprotein levels), 2) electrolytes profile (Sodium, Potassium, Chloride, Bicarbonate, 3) Urea) and 4) Creatinine levels. Furthermore, there was a significant reduction in the urea levels of treated animals and marked but insignificant reduction in the total cholesterol level and increase in High Density Lipoprotein at P

This study was conducted to evaluate the hematological and serum biochemical parameters of growing New Zealand White (NZW) rabbits administered Panax ginseng extracts (PGEs). A total of 48 NZW growing male (M) and female (F) rabbits (24 each) were used and the experiment lasted for 28 days. It was carried out in a randomized complete block design (RCBD), made up of T1 (0.0 mg/ml of PGEs), T2 (200.0 mg/ml of PGEs), and T3 (400.0 mg/ml of PGEs) which was replicated thrice and sex served as the block. Packed cell volume (PCV) (34.03%), red blood cell (RBC) (5.52 × 106/μl), white blood cell (WBC) (7.60 × 103/μl), and heterophil (34.00%) counts of the growing male NZW rabbits were significantly (P

There is no known drug that has prevented or cured reoccurrence of epileptic seizures, hence the need to explore and compare natural remedies that are employed in folkloric treatment of convulsion. The aim of the present was to assess comparatively, the anticonvulsant activity of methanol extracts of Crinum jagus and Solanum indicum. The crude methanol extracts of the bulbs and fruits of C. jagus and S. indicum, respectively, obtained by 72 h maceration in methanol. The extracts were evaluated for anticonvulsant activity using mice and electroconvulsive shock equipment (Ugobasile ECT UNIT 7801). Four groups of six animals each were used for each extract. Group I received no extract serving as control in both cases. Groups II, III and IV received varying concentrations (32.50, 25.50; 41.50, 51.50; and 64.50, 112.50 mg/kg) for C. jagus and S. indicum respectively. All medicaments were protective against electrically induced convulsion. C. jagus showed protection after administration of 64.50 mg/kg body weight while S. indicum was at 112.50 mg/kg body weight. The two extracts justified their use in traditional and folkloric setting with the bulb extract of C. jagus possessing greater anticonvulsant effect than the fruit extract of S. indicum.

BACKGROUND: Despite improvements in multidisciplinary management, patients with biliary tract cancer have a poor outcome. Only 20% of patients are eligible for surgical resection with curative intent, with 5-year overall survival of less than 10% for all patients. To our knowledge, no studies have described a benefit of adjuvant therapy. We aimed to determine whether adjuvant capecitabine improved overall survival compared with observation following surgery for biliary tract cancer.METHODS: This randomised, controlled, multicentre, phase 3 study was done across 44 specialist hepatopancreatobiliary centres in the UK. Eligible patients were aged 18 years or older and had histologically confirmed cholangiocarcinoma or muscle-invasive gallbladder cancer who had undergone a macroscopically complete resection (which includes liver resection, pancreatic resection, or, less commonly, both) with curative intent, and an Eastern Cooperative Oncology Group performance status of less than 2. Patients who had not completely recovered from previous surgery or who had previous chemotherapy or radiotherapy for biliary tract cancer were also excluded. Patients were randomly assigned 1:1 to receive oral capecitabine (1250 mg/m 2 twice daily on days 1–14 of a 21-day cycle, for eight cycles) or observation commencing within 16 weeks of surgery. Treatment was not masked, and allocation concealment was achieved with a computerised minimisation algorithm that stratified patients by surgical centre, site of disease, resection status, and performance status. The primary outcome was overall survival. As prespecified, analyses were done by intention to treat and per protocol. This study is registered with EudraCT, number 2005-003318-13.FINDINGS: Between March 15, 2006, and Dec 4, 2014, 447 patients were enrolled; 223 patients with biliary tract cancer resected with curative intent were randomly assigned to the capecitabine group and 224 to the observation group. The data cutoff for this analysis was March 6, 2017. The median follow-up for all patients was 60 months (IQR 37–60). In the intention-to-treat analysis, median overall survival was 51·1 months (95% CI 34·6–59·1) in the capecitabine group compared with 36·4 months (29·7–44·5) in the observation group (adjusted hazard ratio [HR] 0·81, 95% CI 0·63–1·04; p=0·097). In a protocol-specified sensitivity analysis, adjusting for minimisation factors and nodal status, grade, and gender, the overall survival HR was 0·71 (95% CI 0·55–0·92; p=0·010). In the prespecified per-protocol analysis (210 patients in the capecitabine group and 220 in the observation group), median overall survival was 53 months (95% CI 40 to not reached) in the capecitabine group and 36 months (30–44) in the observation group (adjusted HR 0·75, 95% CI 0·58–0·97; p=0·028). In the intention-to-treat analysis, median recurrence-free survival was 24·4 months (95% CI 18·6–35·9) in the capecitabine group and 17·5 months (12·0–23·8) in the observation group. In the per-protocol analysis, median recurrence-free survival was 25·9 months (95% CI 19·8–46·3) in the capecitabine group and 17·4 months (12·0–23·7) in the observation group. Adverse events were measured in the capecitabine group only, and of the 213 patients who received at least one cycle, 94 (44%) had at least one grade 3 toxicity, the most frequent of which were hand-foot syndrome in 43 (20%) patients, diarrhoea in 16 (8%) patients, and fatigue in 16 (8%) patients. One (INTERPRETATION: Although this study did not meet its primary endpoint of improving overall survival in the intention-to-treat population, the prespecified sensitivity and per-protocol analyses suggest that capecitabine can improve overall survival in patients with resected biliary tract cancer when used as adjuvant chemotherapy following surgery and could be considered as standard of care. Furthermore, the safety profile is manageable, supporting the use of capecitabine in this setting.FUNDING: Cancer Research UK and Roche.

BackgroundUniversal antiretroviral therapy (ART), as per the 2015 WHO recommendations, might reduce population HIV incidence. We investigated the effect of universal test and treat on HIV acquisition at population level in a high prevalence rural region of South Africa.MethodsWe did a phase 4, open-label, cluster randomised trial of 22 communities in rural KwaZulu-Natal, South Africa. We included individuals residing in the communities who were aged 16 years or older. The clusters were composed of aggregated local areas (neighbourhoods) that had been identified in a previous study in the Hlabisa subdistrict. The study statisticians randomly assigned clusters (1:1) with MapInfo Pro (version 11.0) to either the control or intervention communities, stratified on the basis of antenatal HIV prevalence. We offered residents repeated rapid HIV testing during home-based visits every 6 months for about 4 years in four clusters, 3 years in six clusters, and 2 years in 12 clusters (58 cluster-years) and referred HIV-positive participants to trial clinics for ART (fixed-dose combination of tenofovir, emtricitabine, and efavirenz) regardless of CD4 cell count (intervention) or according to national guidelines (initially ≤350 cells per μL and FindingsBetween March 9, 2012, and June 30, 2016, we contacted 26 518 (93%) of 28 419 eligible individuals. Of 17 808 (67%) individuals with a first negative dried blood spot test, 14 223 (80%) had subsequent dried blood spot tests, of whom 503 seroconverted after follow-up of 22 891 person-years. Estimated HIV incidence was 2·11 per 100 person-years (95% CI 1·84–2·39) in the intervention group and 2·27 per 100 person-years (2·00–2·54) in the control group (adjusted hazard ratio 1·01, 95% CI 0·87–1·17; p=0·89). We documented one case of suicidal attempt in a woman following HIV seroconversion. 128 patients on ART had 189 life-threatening or grade 4 clinical events: 69 (4%) of 1652 in the control group and 59 (4%) of 1367 in the intervention group (p=0·83).InterpretationThe absence of a lowering of HIV incidence in universal test and treat clusters most likely resulted from poor linkage to care. Policy change to HIV universal test and treat without innovation to improve health access is unlikely to reduce HIV incidence.FundingANRS, GiZ, and 3ie.

Background: Diabetes is an umbrella term for a number of metabolic diseases that affects the body’s ability to control blood glucose levels. The hormone insulin is produced in the pancreas, and helps to metabolize glucose for energy. Diabetes occurs when insulin production is impaired. Objective: The present study was to examine the effect of smoking on type 2 diabetic patients visiting Federal Medical Centre. Materials and Methods: The sample size was 97 respondents while the major instrument for data collection was a well constructed, validated and reliably tested questionnaire, including observations made on secondary existing record data of the hospital. Data obtained was analyzed using frequency distribution, means, standard deviation and fisher’s exact test. Results: Results of the study indicated that majority of the diabetic patients 78(80.4%) were non smokers, while 8(8.2%) and 11(11.3%) respondents were current and past smokers respectively. Maintainance of good glycaemic control should be considered as the therapeutic goal for prevention of diabetic complications. Conclusion: A comprehensive programmes should be initiated to prevent smoking in type 2 diabetic patients such as: regular physical activity, health education on diabetes, smoking cessation, home blood sugar monitoring - minimize smoking impact to body organs that may lead to many complications. Asian Journal of Medical Science, Volume-5(3) 2014: 63-71 DOI: http://dx.doi.org/10.3126/ajms.v5i3.8950

Background and context: Prostate cancer is the commonest cancer affecting Nigerian men, with worse outcome compared with men from the developed world. There is limited public awareness about prostate cancer in Nigeria. Oga Blue 4 Prostate Awareness (OB4PA) was created by a consortium of Nigerian nonprofits for prostate cancer advocacy (PCA). Aims: -Design PCA using videos, printed brochure and social media -Implement PCA in five Nigerian states -Evaluate the reach and impact of PCA campaign Program/Policy process: Community-based participatory process, involving the medical community, prostate cancer survivor, and the public was used. Multimedia teaching was used to enhance learning and retention; social media was used to engage groups and individuals. Content development involved iterative consultation among project leaders, medical experts and target audience, often on social media. High-quality teaching videos were recorded in English and Nigerian Pidgin languages. Videos ensured consistency and ease of broadcast. Videos were accessed by OB4PA partners through cloud computing (OneDrive). Facebook was used to promote the campaign, engage local audience, and for Facebook Live presentation. Local health professionals projected the video in appropriate language to audiences in religious and community groups. Brochure detailing clinical features and local service providers was distributed. Audience evaluation was obtained following each interaction. Outcomes: In 6 weeks, 20 presentations were made to 1800 persons. The Facebook Live presentation had 1500 views, reached 9302 people and was shared 107 times. A total of 25 Facebook posts were made, resulting in 628 like, 1908 video views, 160 shares, and reached 14,222 people. Almost all participants had positive feedback on the free and detailed advocacy. Most questions focused on the causes and prevention of prostate cancer, especially on the use of nutritional supplements. What was learned: Cloud computing enabled us to have one presenter; this eliminated the need to find a presenter for each organization. Audience appreciated simplified videos used in explaining the disease process and need for personalized early detection. Facebook live presentation attracted the most reactions on social media, with most comments showing that people liked the intervention. Audience feedback showed that adding advocacy cellphone video by a survivor helped demystify prostate cancer. Having the main presentation video in different file formats and sizes enhanced sharing on social media, as most Nigerians access the Internet on cell phones. Reliable access to projectors was challenging, especially in remote areas. Overall, use of cloud computing and social media were crucial in the success of the PCA project. Lessons from OB4PA informed the design of the current We Can, I Can Conquer Cervical Cancer Awareness project in Nigeria.

To the Editors: Recent guidance on intermediate care for chronic obstructive pulmonary disease (COPD) highlights the invaluable role of “early assisted discharge” and “hospital at home” in improving patient care and reducing length of hospitalisation for exacerbations 1. We would like to draw on our novel local experience to suggest a potentially useful addition to such services: namely, a largely patient-triggered “in-reach” service for selected COPD patients from the community. Southampton has been identified as a COPD “hotspot” in the southern part of the UK 2. Local COPD management is now largely coordinated through a multidisciplinary service called The Respiratory Centre (TRC) at …