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Made available in DSpace on 2022-04-28T17:30:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-28 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Introduction: Gingival recession (GR) is a relevant clinical condition due to its high prevalence worldwide, which leads to aesthetic demands and dentin hypersensitivity. Collagen matrices have been associated with different designs of coronally advanced flaps (CAFs) to treat GR defects. However, the literature lacks long-term follow-up of this treatment option. The aim of this study is to present the long-term follow-up (up to 7 years) of two GR defects (associated or not with noncarious cervical lesion) treated with a CAF and a collagen matrix (CM). Case presentation Case 1 underwent a CAF associated with a CM to treat a single GR defect. After 7 years, Case 1 presented with 3 mm of recession reduction, which corresponds with the 85.7% of root coverage. Case 2 presented two GR defects associated with noncarious cervical lesions (NCCLs). The NCCLs were partially restored with resin composite and then underwent a modified CAF for multiple defects and a CM. After 5 years of follow-up, Case 2 presented with 1.5 and 2.5 mm of recession reduction, which corresponds with the average 83.3% defect coverage. Conclusion These two cases may show that CMs can provide long-term stable outcomes in the treatment of GR defects. Fed Univ Pernambuco UFPE, Dept Prosthesis & Surg Orofacial, Recife, PE, Brazil Sao Paulo State Univ, Inst Sci & Technol, Div Periodont, UNESP, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil Private Educ Inst, Sao Paulo, Brazil Sao Paulo State Univ, Inst Sci & Technol, Div Periodont, UNESP, Av Engn Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, Brazil CAPES: 001 CNPq: 304269/2019-0

Objective To review different modes of application of hydroxyapatite for treatment of dentin hypersensitivity via dentinal tubule occlusion. Methods Literature search for the systematic review was done using key words 'hydroxyapatite and dentin hypersensitivity', 'hydroxyapatite and dentinal tubule occlusion', 'hydroxyapatite and dentin permeability', and 'dentinal tubule occlusion' on Pubmed, Science Direct and Web of Science databases for articles published over 10 years, from 2009 to 2018. Results Of the 132 research articles initially identified, 97(73.5%) related to the 10-year study period. After detailed screening, 16(16.5%) studies were included. The results of in vitro studies showed that application of hydroxyapatite caused dentinal tubule occlusion leading to reduction in dentin permeability and reduction in dentin hypersensitivity. Conclusions Hydroxyapatite has the potential to reduce dentin hypersensitivity via dentinal tubule occlusion within 2-8 weeks.

Made available in DSpace on 2022-05-01T10:19:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: Xenogeneic matrices (XMs) have been increasingly used for root coverage procedures. This study compared the use of two types of XM (collagen matrix [CM] and xenogeneic acellular dermal matrix [XDM]) associated with the coronally advanced flap technique (CAF) to treat single gingival recessions. Methods: Seventy-five patients presenting single RT1 gingival recession were treated by CAF (control group, n = 25), CAF+CM (test group 1, n = 25), or CAF+XDM (test group 2, n = 25) and completed 6-month follow-up. Clinical, patient-centered, and esthetic assessments were performed and intra- and intergroup differences were analyzed. Results: At 6 months, the mean recession reduction for CAF, CAF+CM, and CAF+XDM was 2.4 ± 0.8 mm, 2.4 ± 0.9 mm and 2.1 ± 0.8 mm, respectively (P > 0.05). The corresponding mean percentage of root coverage was 78.9% ± 26.2% for CAF, 78.0% ± 28.5% for CAF+CM, and 65.6% ± 26.9% for CAF+XDM (P > 0.05). Dentin hypersensitivity and esthetic conditions showed significantly improvements in all groups. Test groups presented significant gains in gingival thickness (GT; CAF+CM: 0.4 ± 0.3 mm; CAF+XDM: 0.4 ± 0.2 mm) compared to the control group (CAF: 0.0 ± 0.1 mm; P

The main objective of this study was to enhance the blockage of dentinal tubules using nanobioglass in the presence of diode (980 nm) and Nd:YAG lasers in order to reduce permeability and dentin hypersensitivity.Thirty-six dentinal samples were randomly divided into 6 subgroups (n = 6): (A) control, (B) diode laser (980 nm, 3-W), (C) Nd:YAG laser (1064, 1.0-W), (D) nanobioglass, (E) nanobioglass + diode laser (980 nm), (F) nanobioglass + Nd:YAG laser. The average number of open dentinal tubules was qualitatively and quantitatively evaluated by scanning electron microscopy (SEM). Data were evaluated by SPSS software version 22, Kruskal-Wallis test, and Mann-Whitney tests with Bonferoni's correction (α = 0.008).Based on the results of Mann-Whitney test, there was a significant difference in the mean number of open dentinal tubules between the control group and the other groups (p 0.008). However, the difference among the other groups was not statistically significant (p 0.008).Findings of this study showed that high-power laser radiation, such as Nd:YAG and diode (980 nm) alone or with nanobioglass, has a significant effect on the blockage of dentinal tubules.Introduction of non-invasive methods with long-term and lasting effect on reducing pain and discomfort caused by dentin hypersensitivity.

Background: Dentin hypersensitivity is described as “short sharp pain arising from exposed dentin in response to various stimuli typically thermal, evaporative, tactile, osmotic or chemical and cannot be ascribed to any other form of dental defect or disease.” Dentine hypersensitivity can be treated with various desensitizing agents ,where oral rinse has a promising effect in delivering the therapeutic effect all over the tooth surface including interproximal areas, as they can flow and reach areas where toothpastes are not much effective, hence the occlusion of dentinal tubules using Ayurdhantam medicated an herbal mouthwash was evaluated. Methodology: Twenty such dentinal blocks were obtained; the surfaces of each dentin block were polished using a silicon carbide disc for 30 s to create a basic smear layer. The smear layer is finally detached by applying 17% EDTA solution onto the dentin blocks for 5 min to ensure the patency of the dentinal tubules, following which they were rinsed with distilled water. The specimens were placed into the Ayurdhantam solution (medicated) for 2 min every day for 7 days. The specimens were stored in artificial saliva during the study. The specimens were analyzed under scanning electron microscope (SEM) for the occlusion of dentinal tubules. Results: SEM images showed the partial occlusion of the tubules and a large amount of smear layer. Conclusions: Based on this in vitro experiment carried out, it is shown that Ayurdhantam-medicated mouthwash can be used for treating dentine hypersensitivity effectively.

Dentin hypersensitivity (DH) is characterized by short or transient sharp pain arising from exposed dentin in response to thermal, physical, osmotic, or chemical elements (Holland et al., 1997, West et al., 2011). According to the hydrodynamic theory, hypersensitivity results from the rapid movement of the fluid within the dentinal tubules (Brannstrom et al., 1968). It is imperative to the efficacy of the DH treatment to address the etiological factors of the condition (Femiano et al., 2017). Desensitizing therapies consist of the use of chemical substances, laser irradiation, restorative treatments, periodontal surgeries which aim basically to control the hydrodynamic mechanisms of pain. However, these treatments do not achieve predictable results in all patients and may lose their effect over time (Cartwright, 2014, Zhu et al., 2015, Sole-Magdalena et al., 2017). Bioactive glass materials have been proposed for the treatment of DH (David, 2010, Curtis et al., 2010, Barry et al., 2011, Sauro et al., 2011, Cartwright, 2014, Hall et al., 2017, Bansal and Mahajan, 2017). The mechanism of action of bioactive glass starts when the material is exposed to an aqueous environment. The sodium ions (Na+) in the particles immediately are switched with hydrogen cations (H+ or H3O+). This rapid release of ions allows the calcium ions (Ca+2) in the particle structure, as well as the phosphate ions (PO4-3), to be released from the glass particles. The initial reactions occur in seconds of exposure and the release of the Ca+2 and PO4-3 ions continues as long as the particles are exposed to an aqueous environment. A localized and transient increase in pH occurs during the initial material exposure due to the release of Na+. This increase in pH helps to precipitate the Ca+2 and PO4-3 ions of the particle to form a layer of calcium phosphate Ca(PO4)2. As particle reactions and the deposition of calcium and phosphorus complexes are maintened, the layer crystallizes into hydroxycarbonate apatite which is chemically and structurally equal to biological apatite. The combination of residual particles and the newly formed apatite layer lead to physically occluding the dentin tubules, resulting in remission or pain reduction (Zhong et al., 2002, Zanotto et al., 2004, Hench, 2006). The maintenance of this layer on the dentin surface is a major challenge for the materials existing in the market, besides knowing the effectiveness of them for the treatment of DH. Studies have shown the absence of parameters such as: patient follow-up time, control groups, assessment scale for degree of pain, DH diagnostic methods and form of application of the product (Matranga et al., 2017, Hannigan and Lynch, 2013, Kim et al., 2011, Pandis et al., 2011, Fleming et al., 2013). The use of bioactive materials is a promising proposal for DH due to the the neoformation of a bioactive barrier on dentin surface (Bansal and Mahajan, 2017). They showed the ability to occlude the dentinal tubules and form a mechanically strong layer of hydroxyapatite on the dentin surface, which can resist degradation by repeated acid challenges (Du et al., 2008). Thus, the aim of this systematic review was to assess randomized controlled trials (RCT) carried out in the permanent teeth of adult patients in the DH treatment with bioactive dentrifices, either at-home or in-office.

Objective To investigate the treatment efficacy of low-level light therapy on dentin hypersensitivity. Materials and methods Following the PRISMA guideline, six electronic databases supplemented with bibliographies were searched till December 2020. Two reviewers performed the screenings independently with a reliability assessment. Studies fulfilling the pre-registered eligibility criteria were included for risk-of-bias assessment and data synthesis. Results Thirty-five articles ultimately informed this systematic review based on the eligibility criteria and underwent risk-of-bias assessment (ĸ = 0.86). Quantitative results were deduced by meta-analysis of 20 randomised controlled trials: LLLT showed favourable outcomes compared to placebos for immediate (SMD: 1.09, 95% CI: 0.47 to 1.70), interim (SMD: 1.32, 95% CI: 0.41 to 2.23), and persistent efficacies (SMD: 2.86, 95% CI: 1.98 to 3.74). However, substantial heterogeneity existed among included studies (I2: 64–95%). Regarding comparisons with other desensitising strategies, LLLT showed no significant benefits in DH alleviation over others except fluorides for interim efficacy (SMD: 0.31, 95% CI: 0.10 to 0.52) and persistent efficacy (SMD: 0.45, 95% CI: 0.03 to 0.86). Conclusions This systematic review shows that LLLT has positive immediate, interim, and persistent DH-treatment efficacies compared with placebo. No superior treatment effects of LLLT were observed except fluoride agent use. Further studies are warranted—RCTs with low risk of bias, consistent technical settings, comprehensive assessments, and long follow-up periods. Clinical relevance This systematic review bridges a critical research gap by analysing clinical evidence in the DH-alleviating efficacy of LLLT in comparison with placebo and other in-office desensitising strategies.

Objective: to evaluate the efficacy of non-invasive procedures through biomimetic mineralization of a fluoride gel, bioactive glass materials with and without fluoride and self-organizing biomimetic peptides in the treatment of dentinal hypersensitivity due to early non-carious cervical lesions. Materials and methods: Twenty-eight patients with self-reported history of dentin hypersensitivity were evaluated sequentially for evidence of erosion, abrasion or gingival recession at facial cervical region. Patients were randomly distributed into four treatment groups of seven patients each according to the type of the treatment (group I: Elmex Gele'e, group II: BioMin™ F, group III: BioMin™ C group IV: CurodontTM D’senz). The teeth were subjected to five follow up periods (baseline, after 3,6,9 and 12 weeks) and assessed in response to evaporative air-blast stimulus utilizing Schiff Sensitivity and Visual Analogue Scales. Electrical test was performed utilizing electrical pulp tester. The extent of tubule occlusion was examined under scanning electron microscope using negative replica of randomly selected patients. Results: All the tested groups showed different degrees of relieving dentinal hypersensitivity symptoms. The difference between the studied groups was statistically significant. Under SEM, group II, III and IV showed complete tubule occlusion. However, group I showed partial tubule occlusion. Conclusions: Elmex® gelee, BioMin™ F, BioMin™ C and Curodont™ D’senz were effective to manage dentin hypersensitivity related to early non-carious cervical lesions based on the biomimetic mineralization concept that has focused on permanent management of dentin hypersensitivity. Keywords: Fluoride, Bioactive glass, self-organizing biomimetic peptides, non-carious cervical lesions, dentin hypersensitivity.

Background Dentin hypersensitivity (DH) is a sharp toothache that influences a patients' oral health-related quality of life. Oral dentifrices have been marketed for pain relief within a minute for DH. The permanent management of DH is being investigated with the remineralisation potential of bioactive agents in dentinal tubules. This study investigated the relief from pain in DH in one minute after applying over the counter (OTC) dentifrices with Pro-Argin™ and strontium acetate and directly compared them with fluoro-calcium phospho-silicate (FCPS)-based dentifrices for immediate and sustained inhibition of painful stimulus provoking DH. Methods A randomised, controlled, triple-blinded clinical trial was conducted with 140 participants clinically diagnosed with DH and equally randomized into four groups with parallel treatment assignment of FCPS, Pro-Argin™, 8% strontium acetate, and sodium fluoride-based OTC dentifrices, and tested for DH with air blast, mechanical, and water jet stimuli on SCHIFF cold air sensitivity scale (SCASS) and visual analogue scale (VAS) at interim efficacy intervals of one minute, three days, two, four, and six weeks, subsequently. Results A total of 128 participants completed the trial. All the treatment groups showed statistically significant improvement in DH with p p = 0.499). FCPS showed the highest reduction in DH on SCASS and VAS for waterjet stimuli with mean scores of (0.97 ± 0.68) (1.80 ± 1.73) and Pro-Argin™ on VAS for mechanical stimuli with mean scores of (2.15 ± 1.92) in six weeks. Conclusion OTC dentifrices with Pro-argin™ and strontium acetate are effective for immediate pain relief from DH, and FCPS could be the best possible treatment option for long term management of DH. Trial registration ID: NCT04249336 (https://clinicaltrials.gov/ct2/show/NCT04249336), Date of Registration: January 30, 2020 (Retrospectively registered).

AIM The aim of the present study was to compare the capacity of three dissimilar commercially accessible desensitizing products for the occlusion of dentinal tubules using a scanning electron microscope (SEM). MATERIALS AND METHODS The nondiseased human mandibular premolar teeth, ninety in number, were amassed for this study. The blocks subsequently made were subjected to polishing using abrasive paper to facilitate dentinal surface exposure. The samples were randomly allocated to one of the following three groups: Group I, samples to be treated with Admira Protect; Group II, samples to be treated with MI paste, Group III, samples to be treated with Remin Pro. Photomicrograph of every sample was taken to evaluate the occlusion of the dentin tubule under 2000× magnification of the SEM. RESULTS MI paste exhibited the greatest dentin tubular occlusion with a value of 2.746 ± 0.530 followed by Admira Protect (3.498 ± 0.202) and Remin Pro (4.594 ± 0.364) in that order. Amid the various desensitizing materials used, statistically significant differences (p

Dentin hypersensitivity (DH) is characterized by pain caused by an external stimulus on exposed dentin. Different therapeutic approaches have been proposed to mitigate this problem; however, none of them provide permanent pain relief. In this study, we synthesized and characterized experimental bioactive glasses containing 3.07 mol% SrO or 3.36 mol% K2 O (both equivalent to 5 wt% in the glass), and evaluated their effect on dentin permeability to verify their potential to treat DH. The experimental materials were characterized by field-emission scanning electron microscopy, Fourier transform infrared spectroscopy, micro-Raman spectroscopy, and X-ray diffraction to confirm the respective structures and chemical compositions. The reduction in the hydraulic conductance of dentin was evaluated at the three stages: minimum permeability; maximum permeability (24% ethylenediaminetetraacetic acid [EDTA] treatment); and final dentin permeability after treatment with the bioactive glasses. They all promoted a reduction in dentin permeability, with a significant difference for each sample and posttreatment group. Also, a significant reduction in dentin permeability was observed even after a simulated toothbrushing test, demonstrating effective action of these materials against DH. Besides, incorporating 3.07 mol% SrO was a positive factor. Therefore, strontium's desensitizing and re-mineralizing properties can be further exploited in bioactive glasses to promote a synergistic effect to treat DH.

Objectives This study aimed to test the efficacy of two experimentally produced eggshell-based toothpastes on dentinal tubules occlusion. Materials and Methods Two experimental eggshell toothpastes, based on natural ingredients, with or without titanium dioxide nanoparticles (TNPs) were produced and coded “TNPs eggshell toothpaste [TNPsESTP]” or “eggshell toothpaste [ESTP],” respectively. Mid-coronal dentin discs, from 28 human extracted molar teeth, etched with 37% phosphoric acid for 60 seconds to simulate the hypersensitive dentin, were randomly divided into four groups—G1: no treatment (negative control), G2: ESTP treated, G3: TNPsESTP treated, and G4: Biorepair treated (positive control). All treated discs were brushed for 2 weeks and 1 month using a toothbrush simulator at 40 mm/s. Dentinal tubules occlusion was studied using a cross-polarization optical coherence tomography (CP-OCT) and scanning electron microscopy (SEM). Statistical Analysis Unpaired t-test was conducted using GraphPad software (San Diego, California, United States). Continuous variables were expressed as means ± standard deviation and p < 0.05 was considered statistically significant. Results From CP-OCT, a marked increase in surface reflectivity of dentin was observed after brushing with tested toothpastes. ESTP and NPsESTP showed higher or comparable grayscale values than Biorepair indicating increase in surface density of dentin. From SEM, at 2 weeks, ESTP showed comparable number of completely occluded dentinal tubules to Biorepair. TNPsESTP showed significantly lower numbers of CODT than Biorepair. At 1 month, the number of CODT was difficult to count for all treated groups. Both ESTP and TNPsESTP showed significantly higher numbers of partially occluded dentinal tubules than Biorepair. Conclusion ESTP or TNPsESTP could offer a cost-effective substitute for the treatment of dentin hypersensitivity.

The aim of the present study was to assess the duration of dentin tubule occlusion by the calcium phosphate precipitation (CPP) method in the vital teeth of beagle dogs. Vital teeth were treated using the CPP method, potassium oxalate, or a bonding agent (Liner bond II) after cavity preparation and acid etching. The dentin tubules of all groups, except for the bonding agent, opened more widely with time in the absence of plaque control. Dentin tubules treated with the CPP method were open and no precipitate remained in the absence of plaque control. Differences were observed in dentin tubule occlusion when plaque control was achieved by daily tooth brushing. The majority of dentin tubules were occluded with an apatitic precipitate seven days after the CPP method with plaque control. The present results demonstrated that the CPP method is useful with proper plaque control.

Aim This study aims to evaluate the efficacy of avian eggshell slurry as a desensitizing agent compared to casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing Tooth Mousse. Materials and methods Eggshell powder was prepared and characterized using a scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDX). Forty dentin disks were immersed in 6% citric acid for 2 min to simulate hypersensitive dentin (baseline). Disks were then divided into two groups (n = 20) according to treatment received: eggshell slurry and commercially available Tooth Mousse desensitizing agent. Each group was further divided into two subgroups (n = 10). In subgroup 1, dentin discs were immersed in artificial saliva for four weeks whereas, in subgroup 2, discs underwent acid challenge. Environmental scanning electron microscope (ESEM) analysis and hydraulic conductance test were utilized to calculate dentinal tubules occlusion and reduction of dentin permeability percentages, respectively. Statistical analysis was performed using SPSS 22.0 at a significance level p ≤ 0.05. Results After application of desensitizing agents, the eggshell slurry group showed a significantly higher percentage of dentinal tubules occlusion compared to the Tooth Mousse group, whereas no significant difference existed in the percentage reduction of dentin permeability between both groups. After both immersion protocols, the eggshell slurry significantly occluded more dentinal tubules compared to Tooth Mousse. On the contrary, results of percentage reduction of dentin permeability revealed no significant difference between both subgroups after acid challenge. After artificial saliva immersion, Tooth Mousse showed a significantly higher percentage reduction of dentin permeability compared to the eggshell slurry. Conclusion Avian eggshell slurry can effectively occlude open dentinal tubules compared to commercially available Tooth Mousse desensitizing agent. Clinical significance Avian eggshell slurry can be considered a promising material for the treatment of dentin hypersensitivity. How to cite this article: El Tahlawy AA, Saba DA, Bakir NG. Avian Eggshell Slurry as a Dentin Desensitizing Agent: An In Vitro Assessment Using Two Techniques. J Contemp Dent Pract 2021;22(5):532-537.

The effects of most clinical treatments for dentin hypersensitivity are not long-lasting. To overcome the defects, the mesoporous silica nanoparticles and silver nanoparticles entered the field of oral materials. This study aimed to synthesize a novel, low-cytotoxic dentin desensitizer and investigate its occlusion effects on dentinal tubules. The biphasic stratification approach, a chemical reduction method, and the Stöber method were used to synthesize silver nanoparticle-loaded and nonporous silica-encapsulated mesoporous silica (Ag-MSNs@nSiO2), which was a noncrystalline structure with an average size of approximately 128 nm and a silver content of 3.506%. Atomic absorption spectrometry and the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide cell viability assay showed that Ag-MSNs@nSiO2 slowly released silver ions and had nearly no cytotoxicity. An electron microscope was used to observe the blocking effects on the dentinal tubules of sensitive tooth disc models, which were randomly divided into the following four groups: a deionized water group, a 5.9 M silver nitrate solution group, an Ag-MSNs@nSiO2 group, and a Gluma desensitizer group. There were no significant differences in the relative area of open dentinal tubules between the Ag-MSNs@nSiO2 group and the Gluma desensitizer group (P > 0.05). Detection of protein structures showed that multilevel structures of bovine serum albumin in dentin tubules were significantly changed by silver ions from Ag-MSNs@nSiO2. These results suggest that nearly noncytotoxic Ag-MSNs@nSiO2 was successfully synthesized by a series of methods. Ag-MSNs@nSiO2 occluded dentin tubules immediately and effectively. Moreover, the blockage effects may be enhanced and maintained by continuous condensation of proteins in dentinal tubules.

Background: Gingival recession is associated with dentin hypersensitivity, unesthetic appearance, and carious or noncarious cervical lesions. Orthodontic treatment, gingival thickness (GT), and keratinized tissue width (KTW) play roles in gingival recession etiology. The study is aimed to compare the prevalence of gingival recession in the mandibular incisor region of orthodontically-treated females with untreated controls, and to identify if there is any correlation among the GT, KTW, and gingival recession. Subjects and Methods: A total of 150 Saudi females were enrolled in this study. They were categorized into the treatment group (n = 75) and control group (n = 75). Background characteristics and clinical periodontal parameters including the plaque index, gingival index, GT, KTW, gingival recession length (GRL), and gingival recession width (GRW) were recorded and compared between groups, and any associations were identified. Results: Among the orthodontically-treated females, 31 (41.33%) had at least one mandibular incisor with gingival recession, in contrast to 18 (24%) of the controls. Pearson's correlation analysis indicated a significant positive correlation between the GT and KTW and between the GRL and GRW (P < 0.001). In addition, a significant negative correlation was observed between the KTW and GRL and between the KTW and GRW (P < 0.001). However, no significant correlation was found between the GT and GRL or between the GT and GRW. Conclusions: Our findings indicated that orthodontic treatment is a predisposing factor for the development or progression of gingival recession, particularly in females with a narrow KTW.

Objectives: To evaluate the efficiency and stability of Nano seashell, Sodium Fluoride and Signal Complete 8 in treatment of dentine hypersensitivity. Methods: Forty freshly extracted premolar teeth were collected, disinfected, and sliced into 1.0± 0.1mm thickness. The dentin discs were randomly divided into two main groups of 20 discs each. Group 1(n=20): Dentin discs subjected to treatment not followed by acid challenge. Group 2 (n=20): Dentin discs subjected to treatment followed by acid challenge. Each group was further subdivided into 4 subgroups of 5 discs each according to the treatment used; Subgroup A (Control group): discs received no treatment; Subgroup B: discs treated with Sodium Fluoride paste; Subgroup C: discs treated with Nano Seashell paste; Subgroup D: discs treated with Signal Complete 8 toothpaste. Evaluation of dentinal tubules obliteration was done under scanning electron microscope. Results: There was a significant change in the diameter of tubules and percentage of occlusion for group 1. Group 2 (subjected to acid challenge after treatment) has shown high stability for subgroup B (Sodium Fluoride), followed by subgroup D (Signal Complete 8) then subgroup C (Nano seashell paste). Conclusion: Nano seashell and Sodium Fluoride caused significant increase of occluded dentinal tubules than Signal complete 8 but the mineral deposits formed by Sodium Fluoride showed higher resistance to acidic environment than those formed by Nano seashell.

Purpose The immediate application of a dentin-bonding agent after tooth preparation and before impression-taking (immediate dentin sealing [IDS]) has been suggested to provide several advantages concerning bacterial microleakage, hypersensitivity, and bonding quality. We reviewed the literature and clarified certain aspects related to each step of IDS application. Materials and Methods The search strategy comprised an electronic research in MEDLINE, Cochrane, Ovid and Scopus for studies published from January 1990 to December 2020 regarding the IDS technique and including both in vitro and clinical studies. Results After exclusion of irrelevant or duplicate articles, 88 articles focusing on aspects of the IDS technique were assessed. IDS seems to be advantageous with regard to bond strength, gap formation, bacterial microleakage, and dentin hypersensitivity. However, issues arising from interaction with impression materials, the provisional phase, and conditioning methods before cementation require further investigation. Conclusion There are no documented reasons preventing clinicians applying IDS in their everyday practice. On the contrary, the presented technique seems to be beneficial in certain aspects regarding indirect restorations.

Purpose: To assess the effectiveness of calcium sodium phosphosilicate in reducing dentin hypersensitivity compared to other dentin tubule occluding molecules. Methods: A structured research question was formulated, and an electronic search of available literature was carried out via PubMed, Google Scholar, and Scopus. A hand search as well as a gray literature search were also carried out. The search produced a total of 67 articles. Of these, only eight articles were eligible to be included in our review. Risk of bias and study quality were checked using Cochrane tool. The review was registered in The International Prospective Register of Systematic Reviews (PROSPERO) under registration number CRD42018096200. Results: The results show a reduction in dentin hypersensitivity with calcium sodium phosphosilicate compared to many other molecules. However, nanohydroxyapatite showed a better desensitizing effect compared to Novamin. Conclusion: According to the available evidence, 5 % calcium sodium phosphosilicate containing toothpaste is more effective reducing dentin hypersensitivity compared to many other dentinal tubule occluding molecules.

This five-phase, single-blind, crossover in situ trial evaluated the effects of desensitizing or anti-erosive toothpastes on dentin erosive wear and tubule occlusion. Some characteristics such as relative dentin abrasivity (RDA), viscosity (V), cytotoxicity, and fluoride and calcium (Ca) availability of the toothpastes were also tested. Samples were positioned on removable intraoral appliances from 15 volunteers (n=4), according to the groups, C-control (0 ppm fluoride), F-sodium monofluorophosphate (MFP), A-MFP and arginine+calcium carbonate, CSP-calcium sodium phosphosilicate, and CS-MFP and calcium silicate+sodium phosphate. Erosion-abrasion cycling was performed (1% citric acid, 2min, 6×/day; toothbrushing, 5s, 2×/day). Surface loss (SL) was evaluated by optical profilometry. Environmental scanning electron microscopy images (ESEM) counted the number of open dentin tubules (ODTs). Data were statistically analyzed (α=0.05). There were no significant differences in SL among groups (p=0.468). The ODT for CSP, CS, and A groups were significantly lower than the control. CSP and CS presented significantly less ODT than F, but they did not significantly differ from A. F and CS were more abrasive than all the other toothpastes; CSP was more abrasive than A, and C was the least abrasive. CSP presented higher V (p

Noncarious cervical lesions (NCCLs) are a common clinical finding often linked with dentin hypersensitivity (DH). Aim. The aim of the study was to evaluate the influence of diode laser for the treatment of DH on microleakage of subsequent NCCL restorations. Materials and Methods. Forty-eight extracted human premolars were collected. All teeth received standardized cervical preparation on both the buccal and palatal surfaces and were randomly divided into three groups ( n = 16 ) according to the restorative material used: nanohybrid composite resin (CR), resin-modified glass ionomer (RMGI), and conventional glass ionomer (GIC). The prepared cavities on the palatal surfaces were treated by diode laser using SIROlaser Blue (Sirona Dental Systems, Bensheim, Germany) prior to restoration, while preparations on the buccal surfaces were directly restored. After thermocycling, the teeth were immersed in methylene blue dye for microleakage evaluation under 40x magnification at both occlusal and cervical margins. The Kruskal-Wallis test followed by the Bonferroni tests was conducted to determine inter- and intragroup differences ( P < 0.05 ). Results. All restorative materials tested showed some degree of microleakage with no statistically significantly different scores with or without the use of laser desensitization prior to restorative treatment. Group CR showed the least microleakage, followed by group RMGI, while group GIC showed the highest. Cervical margins showed greater microleakage than the occlusal margins where the difference was statistically significant in the RMGI group without laser pretreatment ( P = 0.006 ) and in both groups CR ( P = 0.02 ) and RMGI ( P = 0.006 ) with the laser pretreatment. Conclusion. Application of diode laser for the treatment of DH prior to the restoration of teeth with NCCL did not affect the microleakage of all the restorative materials tested. All the materials showed some degree of microleakage, which was higher in gingival margins compared to occlusal margins. The resin composite shows the least microleakage among all the tested materials.

This clinical trial aimed to compare the effects of low-level laser therapy (LLLT), Er,Cr;YSGG laser, and fluoride varnish, as compared to the placebo laser on decreasing dentin hypersensitivity (DH). This randomized, double-blinded clinical trial included 60 jaw quadrants in 24 patients who underwent periodontal surgery. The quadrants were randomly assigned to 4 groups and received treatments as follows. Group 1: LLLT with a combination of red and infrared wavelengths, group 2: Er,Cr:YSGG laser (0.25 W and 0.5 W), group 3: fluoride varnish, and group 4: placebo laser. The sensitivity response to the cold spray was recorded using visual analogue scale (VAS) at baseline, immediately, and 1 week post-treatment. The data were analyzed by repeated measures analysis at the significance level of P

Objective: To compare the mean dentin hypersensitivity after using GC tooth mousse versus fluoride varnish in patients with dentinal hypersensitivity due to non-carious cervical lesions. Study Design: Randomized Control Trial. Setting: Watim Dental College, Rawalpindi. Period: February to August 2019. Material & Methods: A total number of 166 patients were enrolled in the study. Informed consent and demographic information were taken. The subjects were randomized by using random number table to minimize bias. Patients were divided into 2 groups. Fluoride varnish (group 1) which is the control group and GC Tooth Mousse which is group 2, both were used 3 times in the first day during the first week. On follow up the tooth sensitivity was noted. The follow up appointment to measure the level of dentinal hypersensitivity was done after the given treatment on the 7th day of the first week. For measuring the dentin hypersensitivity, the Visual Analogue Scale was used. The data that was collected was entered and 1 then it was analyzed on SPSS version 21. Results: In this study mean age of the fluoride varnish group patients was 44.14±8.95 years, in GC tooth mousse group patients was 44.24±9.04 years. The mean pain score of fluoride varnish group patients was 1.98±1.43 while in GC Tooth Mousse paste group patients was 2.39±1.21 (p-value=0.048). Conclusion: Fluoride varnish group showed significantly lower mean dentin hypersensitivity than to GC tooth mousse group in patients with dentinal hypersensitivity with caries free cervical lesions.

Jian Yu,* Luyao Yi,* Rui Guo, Jingmei Guo, Hongye Yang, Cui Huang The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hongye Yang; Cui HuangThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, #237 Luoyu Road, Wuhan, 430079, Hubei Province, People’s Republic of ChinaEmail yanghongye@whu.edu.cn; huangcui@whu.edu.cnBackground: The dentin exposure always leads to dentin hypersensitivity and/or caries. Given the dentin’s tubular structure and low mineralization degree, reestablishing an effective biobarrier to stably protect dentin remains significantly challenging. This study reports a versatile dentin surface biobarrier consisting of a mesoporous silica-based epigallocatechin-3-gallate (EGCG)/nanohydroxyapatite delivery system and evaluates its stability on the dentinal tubule occlusion and the Streptococcus mutans (S. mutans) biofilm inhibition.Materials and Methods: The mesoporous delivery system was fabricated and characterized. Sensitive dentin discs were prepared and randomly allocated to three groups: 1, control group; 2, casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) group; and 3, the mesoporous delivery system group. The dentin permeability, dentinal tubule occlusion, acid and abrasion resistance, and S. mutans biofilm inhibition were determined for 1 week and 1 month. The in vitro release profiles of EGCG, Ca, and P were also monitored.Results: The mesoporous delivery system held the ability to sustainably release EGCG, Ca, and P and could persistently occlude dentinal tubules with acid and abrasion resistance, reduce the dentin permeability, and inhibit the S. mutans biofilm formation for up to 1 month compared with the two other groups. The system provided prolonged stability to combat oral adverse challenges and served as an effective surface biobarrier to protect the exposed dentin.Conclusion: The establishment of the dentin surface biobarrier consisting of a mesoporous delivery system indicates a promising strategy for the prevention and the management of dentin hypersensitivity and caries after enamel loss.Keywords: epigallocatechin-3-gallate, nanohydroxyapatite, mesoporous silica, dentin, biofilm

Resume. Nowadays, aesthetics plays an important role in dentistry due to the increased attention of people to the beauty and attractiveness of the smile, which is known as an effective tool in achieving both personal and professional purposes. Therefore, the whitening of discolored teeth is one of the promising directions in aesthetic dentistry. To date, there is much controversy among dentists about the effectiveness and, the most importantly, the safety of teeth whitening. The prevention of dentin hypersensitivity is among the main tasks associated with the safe and comfortable treatment and follow-up period for patients. In order to avoid the negative consequences of hyperesthesia, the teeth whitening procedure should be finished with remineralizing therapy. Thought there are a large number of techniques and products for the treatment of hyperesthesia, the question of their most appropriate selection and combination is remaining unresolved and is relevant to meet the steadily growing population demands. Purpose: to highlight the latest views on techniques and products for remineralizing therapy in the process of teeth whitening based on the analysis of relevant literature. Results. Numerous bleaching methods are mainly based on applying high concentrations of hydrogen peroxide. At present, the safety of whitening methods remains controversial and thus requiring further research. The problem of improving techniques aimed at preventing the side effects of professional whitening on the oral tissues and organs are remaining relevant. The problems associated with professional whitening include the decreased resistance of hard dental tissues, the appearance of areas of demineralization and hyperesthesia in the hard dental tissues. At present, a wide range of products has been proposed to treat and prevent the development of teeth hypersensitivity. However, the most effective of them, which could be offered to patients after professional whitening have not been identified yet. To prevent and treat dental hyperesthesia effectively, fluoride-containing remineralizing remedies are widely used to diminish dentin sensitivity. There are studies demonstrating the highest incidence of teeth hyperesthesia during light catalytic and chemical bleaching Therapeutic and prophylactic agents, which contain calcium, phosphorus and fluoride ions, which promote dental enamel remineralization, have been proposed. The use of remineralizing gels before the procedure of medical teeth whitening reduces the occurrence of dental hyperesthesia. That is why many manufacturers of bleaching systems have begun to add desensitizing components to their composition. Thus, Opalescence Boost PF gel contains potassium nitrate and fluorine, Philips Zoom whitening gel contains amorphous calcium phosphate, in addition; remineralizing gel Relief ACP with amorphous calcium phosphate is offered to reduce the occurrence of teeth hypersensitivity during whitening procedure. The results of our research have shown the most significant changes expressed by a decrease in organic acids, maximum increase in concentrations of calcium ions in oral fluid, occur when using photo-bleaching system Beyond Polus, while minimal changes are observed when using chemical activation system Opalescence Xtra Boost. The combination of a reminalizign remedies based on zinc-substituted hydroxyapatite carbonate with laser phonophoresis can be recommended be apply after any bleaching techniques and they have been proved to be the most effective. Conclusion. There is a wide range of techniques and products of remineralizing therapy applied in the process of teeth whitening. Various options of remineralizing therapy and applying different compounds reducing teeth sensitivity after teeth whitening procedure are described in the article. Many formulas have been already developed to ensure patient comfort after a whitening procedure, and in particular to prevent the development of hyperesthesia. Some manufacturers incorporate remineralizing remedies in the whitening system that allows dentists to solve the problem of teeth hypersensitivity effectively.

Dentinal hypersensitivity (DH) is a condition that causes patient discomfort. To evaluate the clinical efficacy of Gluma, fluoride varnish and Tetric N-Bond self-etch system in relieving DH immediately and over 30 days following a single topical application. The present randomized clinical trial was conducted on 55 patients with an age range 20–49 years. 70 teeth in total were incorporated and randomly assigned to the three groups. Parameters examined were: Tactile, air blast, and cold stimuli. VAS was used to assess tactile stimulus whereas the Schiff Cold Scale for air blast and cold stimuli. DH was evaluated immediately, at two weeks and 1 month follow up. Gluma showed a statistically significant reduction in DH scores over other materials. It was concluded that Gluma have statistically significant results over other materials in relieving DH immediately and over 30 days following a single topical application.Trial Registration: Clinical Trials.gov Identifier: NCT04351412, King Abdulaziz University Protocol Record 129-09-19. Registered 17 April 2020 – Retrospectively registered. http://ClinicalTrials.gov/NCT04351412.

Inferior alveolar nerve (IAN) injury is usually caused by stretching or crushing of the neurovascular structures and postoperative intra-alveolar hematoma or edema after dental procedures. This results in paresthesia in the ipsilateral chin, lip (vermilion border, skin, and mucosa), and labial or buccal alveolar mucosa of the mandibular anterior teeth. However, there are no reports of sensory alterations in the teeth, especially tooth hypersensitivity, after IAN injury. I report a case in which paresthesia of the lower lip and hypersensitivity of the lower anterior teeth occurred simultaneously after the removal of the third molar that was located close to the IAN. In addition, I discuss the reasons for the different sensory changes between the tooth and chin (skin) after nerve injury from a neurophysiological point of view. Since the dental pulp and periodontal apparatus are highly innervated by the inferior alveolar sensory neurons, it seems necessary to pay attention to the changes in tooth sensitivity if IAN injury occurs during dental procedures.

OBJECTIVE: The objective of this study was to compare the efficacy of dentifrices containing potassium nitrate and strontium chloride in reducing dentinal sensitivity pain. METHODOLOGY: A pilot study was conducted in Islamic International Dental Hospital Islamabad, Pakistan (Duration two weeks). Forty participants were selected, aged between 18-40 years, having dentin hypersensitivity with the minimum score of 4 on Visual Analog Scale. Participants were randomly allocated with dentifrices containing 5% w/w potassium nitrate and 10 % w/w strontium chloride and were asked to use it for 2 weeks and come back for follow up. Statistically significant correlations were found using the Man Whitney test. A pre-designed proforma was used to acquire information about the patient. Sensitivity was determined with the aid of the Visual Analog Scale. RESULTS: There was greater reduction in moderate pain from 85.8% to 71.4% with strontium chloride and from 84.2% to 79% with potassium nitrate. CONCLUSION: 10 % w/w strontium chloride is more effective than the 5% w/w potassium nitrate for the management of dentin hypersensitivity. KEYWORDS: Toothpastes, Sensitivity, Strontium Chloride, Potassium Nitrate HOW TO CITE: Kiani AZ, Hassan S, Rafiq T. Clinical comparison of 5% potassium nitrate and 10% strontium chloride toothpastes in alleviating dentin hypersensitivity following manual scaling: A pilot study. J Pak Dent Assoc 2021;30(1): 56-60.

This study sought to assess intrapulpal temperature rise following two dentin hypersensitivity (DH) treatment protocols using the 810-nm diode laser. In this randomized clinical trial, 45 maxillary and mandibular incisors and premolars were randomly divided into three groups primary phase (no intervention), 1 W diode laser for 10 s (group 1), and 0.5 W diode laser for 60 s (group 2). In all groups, the access cavity was prepared, and the thermocouple was placed in the pulp chamber and fixed by Cavit. Intrapulpal temperature was measured immediately after access cavity preparation and every minute for 10 min in the primary phase. In the second phase group 1, the temperature was measured with 2-s intervals after irradiation for 10 s and group 2, with 15-s intervals after irradiation for 60 s. Data were analyzed using repeated measures ANOVA and t test (P ≤ 0.05). The mean stabilized baseline temperature was 32 °C. The mean temperature rise was 5 °C. In both experimental groups, the stabilized temperature after laser irradiation was significantly higher than the baseline temperature (P 0.05). Time to stabilize temperature after irradiation in group 1 was significantly shorter than that in group 2 (P

Aim: Dentine hypersensitivity is a common oral problem. This pilot study investigated the prevalence of dentine hypersensitivity in the diverse population across four centres of Bihar and Jharkhand. Materials and Methods: A multi-stage, random sampling method was used to investigate the study population. A total of 5622 subjects were examined at four centres in Bihar and Jharkhand. Subjects were divided into 8 age groups. Participants completed a dentine hypersensitivity questionnaire and underwent clinical examination. The diagnosis of dentine hypersensitivity was confirmed. Gingival recession of sensitive teeth was measured by a Williams periodontal probe. Results: Among 5622 subjects, 1253 were found to be suffering from dentin hypersensitivity indicating a prevalence of 22.28%. Females were more prone to dentin hypersensitivity with male to female ratio of 1:1.31. While subjects in age group of 50-59 were predominantly affected, the molars and premolars were commonly affected with dentin hypersensitivity. Conclusion: The prevalence of dentine hypersensitivity in East India was 22.28%, indicating that it is a common condition. For its effective management, public education about the condition and effective treatment of dentine hypersensitivity are required. Clinical Significance: Assessment of prevalence of hypersensitivity in this demographical location would enable the dental care health workers to devise strategies to educate masses about the prevention and management of this disease

Background Dentin hypersensitivity (DH) is defined as an exaggerated sensitivity of vital dentin exposed to thermal, chemical and tactile stimuli. This study aimed to evaluate, through a literature review, the applicability of high- and low-intensity lasers in the treatment of DH for the past 10 years, as well as its therapeutic potential. Material and methods The electronic databases MEDLINE/PubMed and LILACS were searched using the descriptors ("Dentin Sensitivity" OR "Dentin Hypersensitivity") AND ("Low-Level Therapy" OR Laser), for articles published between 2010 and 2020. Only randomized clinical trials with full-text and full case resolution were included. Results We found 187 articles in total, among which 61 were pre-selected and 10 included in this literature review. Conclusions Considering the found results and their possible limitations, high- and low-intensity lasers, associated or not with other therapies, have demonstrated beneficial effects in the treatment of DH, being considered a promising, safe, easy, and effective field of research, reducing pain sensitivity and preserving pulp vitality. Key words:Dentin sensitivity, dentistry, laser.

The underlying dentin could be exposed to a humid atmosphere filled with bacteria if the covering enamel layer is broken because of external chemical and physical conditions. Accordingly, some diseases like bacterial invasion and dentin hypersensitivity often occur, which impact the daily life of patients. The study is aimed at evaluating the occluding effects of mesoporous calcium silicate nanoparticles (MCSNs) on the dentinal tubules in vitro and in vivo, as well as the antibacterial property and drug delivery ability when loaded with chlorhexidine (CHX) in vitro. MCSNs were synthesized according to the standard protocol. After a series of complimentary evaluations in vitro and in vivo, it was found that MCSNs and CHX–MCSNs could continually form apatite-like enamel layers on the exposed dentinal tubules and significantly reduced dentin permeability both in vitro and in vivo. Besides, MCSN and CHX–MCSN possessed low cytotoxicity in vitro, and only mild pulp inflammation was observed in two MCSNs containing groups in vivo. In addition, MCSN loaded with CHX released CHX sustainably and revealed a significant antibacterial effect against E. faecalis in vitro. Therefore, the results suggest that MCSN could be used as a promising biomaterial to occlude the dentinal tubules and carry antibiotics for avoiding further pulp infection.