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23 results on '"F. Pineau"'

1. Acquired factor V inhibitor: Success of steroids in a patient with primary sclerosing cholangitis, ulcero haemorragic rectocolitis, biological Biermer's disease

Author

F. Pineau-Vincent, P. Lemaire, K. Laribi, and P. Toulon

Subjects
Adult, Male, medicine.medical_specialty, medicine.drug_class, Cholangitis, Sclerosing, Clinical Biochemistry, Colonoscopy, Context (language use), 030204 cardiovascular system & hematology, Proctocolitis, Bethesda unit, Gastroenterology, Primary sclerosing cholangitis, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Humans, Prothrombin time, medicine.diagnostic_test, biology, business.industry, Biochemistry (medical), Anticoagulant, Factor V, Hematology, medicine.disease, biology.protein, Steroids, Blood Coagulation Tests, Antibody, business, 030215 immunology
Abstract

We report the case of a 43-years-old Turkish man with acquired deficiency of factor V (FV) diagnosed in a usual screening before a (recto) colonoscopy. In the biologic explorations, activated partial prothrombin time (APTT) was abnormally high and prothrombin time (PT) was low 18 IU/dL with no anticoagulant drugs (the PT was normal 6 months ago). The controlled level of factor V was 3 IU/dL with FV antibodies (9 Bethesda Units/mL). This patient had a previous history of primary sclerosing cholangitis (2000) and ulcero haemorrhagic rectocolitis (2002) and a fortuitous biological Biermer's disease was revealed. Corticosteroids were prescribed at 1 mg/kg/day with decreasing during 6 months, patient had gradual regression of the caused bleeding and FV became greater than 90%, F V antibodies decreased to less than 0.7 Bethesda Units/mL. This case illustrates the presence of FV inhibitor in an autoimmune gastrointestinal context with regression of clinical (caused) signs and antibodies with corticosteroids.

Published
2021

2. Diagnosis and immediate treatment of acquired von Willebrand syndrome revealed by recurrent cerebral hemorrhage

Author

K. Laribi, M Alani, A Legout, A Besancon-Bergelin, P. Lemaire, and F. Pineau-Vincent

Subjects
medicine.medical_specialty, biology, medicine.drug_class, business.industry, Biochemistry (medical), Clinical Biochemistry, Recurrent cerebral hemorrhage, Anticoagulant, Hematology, 030204 cardiovascular system & hematology, Gastroenterology, Monoclonal IgG, Immunoglobulin G.monoclonal, 03 medical and health sciences, 0302 clinical medicine, Acquired von Willebrand syndrome, Coagulation, Internal medicine, medicine, biology.protein, Antibody, business, circulatory and respiratory physiology, 030215 immunology
Abstract

We report the case of a French woman with acquired von Willebrand syndrome who presents recurrent subarachnoid and intra-cerebral hemorrhage since 2012. She had no family or personal bleeding history. In the biologic explorations, APTT was abnormally high with no anticoagulant drugs (it was normal, historically). Two monoclonal IgG and IgM kappa proteins were detected without any lymphoproliferative disorder. Intravenous infusion of immunoglobulin is very effective in AVWS with immunoglobulin G monoclonal gammapathie of undetermined significance. We had a satisfactory correction of coagulation factors for about 30 days. The exploration of APTT is surely essential for the diagnosis and treatment.

Published
2019

3. Un syndrome coronarien aigu après une piqûre d’insecte rampant, un nouveau cas de syndrome de Kounis ?

Author

T. Masseguin, F. Pineau, and P.J. Marianne-Dit-Cassou

Subjects
Gynecology, 03 medical and health sciences, medicine.medical_specialty, 0302 clinical medicine, business.industry, Emergency Medicine, medicine, 030208 emergency & critical care medicine, 030204 cardiovascular system & hematology, Critical Care and Intensive Care Medicine, business
Abstract

Resume Le syndrome de Kounis est un syndrome coronarien aigu (SCA) survenant dans un contexte d’allergie. Le 2 janvier 2014, sur l’Ile de la Reunion, un patient pique au pied par un insecte rampant est victime d’un SCA ST+ complique de troubles du rythme avec arret cardiorespiratoire. Il beneficie d’une thrombolyse pre-hospitaliere. La coronarographie met en evidence une lesion coronarienne pre-existante ; il pourrait s’agir d’un syndrome de Kounis de type 2, avec lesions coronaires quiescentes. Nous resumons ici les differents types de syndrome de Kounis, leur physiopathologie et les specificites de leur prise en charge. Devant ce syndrome peu connu et de diagnostic difficile, nous soulignons l’importance d’un interrogatoire precis, lors de la prise en charge d’une douleur thoracique afin de mettre en evidence un eventuel contact avec des allergenes ; et de realiser un ECG systematique a chaque patient presentant une reaction allergique. Afin de completer le bilan de ce type de syndrome, il serait opportun de realiser un dosage des differents marqueurs de l’anaphylaxie.

Published
2016

4. Impact of an adaptive program for cognitive and emotional deficits (ADACOG program) in multiple sclerosis patients with cognitive impairments

Author

Elisabeth Maillart, Céline Louapre, Julie Socha, Catherine Lubetzki, F. Pineau, C. Papeix, Jean-Christophe Corvol, Rana Assouad, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Adult, Male, Multiple Sclerosis, [SDV]Life Sciences [q-bio], Efficiency, Organizational, Hospital Anxiety and Depression Scale, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Quality of life, Humans, Medicine, Cognitive Dysfunction, Affective Symptoms, Prospective Studies, 030212 general & internal medicine, Depression (differential diagnoses), Aged, business.industry, Multiple sclerosis, Neuropsychology, Cognition, Middle Aged, medicine.disease, Screening questionnaire, Neurology, Anxiety, Female, Neurology (clinical), medicine.symptom, business, 030217 neurology & neurosurgery, Clinical psychology
Abstract

Background Cognitive impairment is frequent in multiple sclerosis (MS), affecting approximately 40 to 70% of patients. We developed a psycho-educational program (ADACOG program) to allow patients to cope with cognitive deficits. The purpose of this exploratory study was to investigate the impact of the ADACOG program on subjective self-reported cognitive impairments, quality of life, anxiety, depression and self-esteem in MS patients. Methods ADACOG program is a psycho-educational program focusing on cognitive and emotional dysfunctions in MS consisting of three modules in small groups lasting two hours every two weeks. Forty-five MS patients with self-reported cognitive impairments and objective cognitive deficits were enrolled consecutively in two groups: (i) the ADACOG group (N = 24) and (ii) the control group (N = 21). Both groups of patients completed questionnaires evaluating self-reported cognitive impairments (Multiple Sclerosis Neuropsychological Screening Questionnaire), quality of life (Multiple Sclerosis Impact Scale), anxiety and depression (Hospital Anxiety and Depression Scale, HAD) and self-esteem (Rosenberg Scale) at inclusion (M0), one month later (M1) and seven months after inclusion (M7). The evolution of outcomes within ADACOG group and between both groups was analyzed. Results The analyses within the ADACOG group showed that patients reported better quality of life and fewer anxiety symptoms at M1 compared to M0 (respectively P = 0.03 and P = 0,04). Moreover, patients presented less subjective self-reported cognitive deficits at M7 compared to M0 (P = 0.003). Score evolution for HAD depression and self-esteem were not significant within the ADACOG group. The change M1-M0 for MSIS-29 and HAD anxiety scores was significantly different between both groups (respectively P = 0.04 and P = 0.008), with improvement of quality of life and anxiety in the ADACOG group. The evolution of scores between groups was not significant for the other outcomes. Discussion This study showed a small effect of a psycho-educational program focusing on cognitive and emotional disorders in MS patients with subjective self-reported cognitive deficits and objective cognitive deficits. Interest of psycho-education focusing on cognition in MS patients is discussed.

Published
2019

5. Abstracts from the Food Allergy and Anaphylaxis Meeting 2016

Author

Georgios Rentzos, Esben Eller, Annick Mercenier, Magna Correia, Pedro Moreira, Linda Verrill, Mário Morais-Almeida, Frauke Schocker, Carsten Bindslev-Jensen, Ulrike Lehnigk, Andreas Frey, Lars Verschuren, Anna-Lena Bramstång Björk, Lene Heise Garvey, Elena Molina, Katrine L. Bøgh, J. L. van der Velde, Montserrat Alvaro, Phil Padfield, Niamh Brosnan, Nelly Gourdon-Dubois, Christian Harwanegg, Manuel Branco Ferreira, Songül Yürek, Amyra Ali Azamar Jácome, Rashid Amin, José Ramón Fernández Lorenzo, Kirsten Beyer, Florin-Adrian Secureanu, Charlotte Bernhard Madsen, Julie Galand, Claudia Pföhler, Rabea Reinert, Heinz Fehrenbach, Anton Hartmann, Maria Salas, Arne Homann, Rand Arnaout, Sarah Lindsley, Nerea Sarmiento Carrera, Patrícia Padrão, Berber Vlieg-Boerstra, Alkerta Ibranji, Benoît Sterling, Maria Auxiliadora Guerrero, Maria L. Baeza, Maurizio Tamburrini, Eva Untersmayr, Tanja Cirkovic Velickovic, Farrukh Sheikh, Sarah Kuntz, Sara Martínez, Kriti Gupta, Maria de Lurdes Torre, Yvonne Vergouwe, Ross Yarham, Faisal R. Bakhsh, Marta Vazquez-Ortiz, João Marcelino, Tullio Frediani, Ricardo Prata, Anna Kaarina Kukkonen, Gustavo Soldateli, Ines Mrakovčić-Šutić, Elodie Drumez, Cristina Ornelas, Maria Vazquez de La Torre, Renata Barros, Agata Szymkiewicz, Aneta Tomaszewska, Stefanie Rohwer, Charlotte Eisenmann, Adriana Muntean, Matteo Moretti, Johanna P. M. van der Valk, Birgit Quinting, Stefan Kabasser, David Gillick, Michał Przybyszewski, Grzegorz Sergiejko, Antonio Jorge Cabral, Alba Pablos-Tanarro, Robert Elfont, Marit Reitsma, Roxana Silvia Bumbăcea, Nelson Rosario, Maria Livia Bernardi, Cristiane N. Santos, Christian Radauer, Sandra Denery-Papini, Geert F. Houben, Nicolette W. de Jong, Marta Anda, Alexander Rohrbach, Teodorikez Wilfox Jimenez-Rodriguez, María Teresa Villalba, Karine Patient, Harmieke van Os-Medendorp, Kathrin Scherer, Marta Goñi Esarte, Svetlan Dermendzhiev, Jossie Garthoff, Michelle M. Epstein, Catherine Bertholet, Bruna Pultrini Aquilante, Didier G. Ebo, Roberta Aina, Jorge Kalil, Petri Kulmala, Lars K. Poulsen, Barbara Ballmer, H. Mary-Lene de Zeeuw-Brouwer, Karin Hoffman-Sommergruber, Winfried Leeman, Anne Miles, Nehad Gomaa, Maria Teresa Costantino, Evelyne Mangodt, Maria Konstantakopoulou, Rosa Jimenez, Attilio Francesco Speciani, Helga Magnusdottir, Inmaculada Sánchez-Machín, H. Kaddouri, Raquel Perez, Astrid Versluis, Anthony E. J. Dubois, Anja Koren Jeverica, Zdenka Barićev-Novaković, Joost Westerhout, A.M. Plaza, Júlio Oliveira, Pierre Lukas, Oksana Matsyura, Giuseppe Pingitore, Mira Silar, Huub F. J. Savelkoul, Urszula Samolinska-Zawisza, Chantal Brossard, Ana Reis Ferreira, Reiko Teshima, Serena Perna, Mariola Dietrich, Dirk Verhoeven, Fiona Kenna, Paloma Poza-Guedes, Cristobalina Mayorga, Pilar Hernandez, Roberto Bernardini, Roberto Berni Canani, Maria Francesca Patria, Mariana Vieru, Julie Locklear, Esther van Twuijver, Marina A. Kiseleva, H. Kim A. Brand, Gabriele Schulz, Ileana-Maria Ghiordanescu, Nikolaos G. Papadopoulos, Danijela Apostolovic, Maria del Mar Folqué, Eva Lasa, Mohammad Al Bahkali, Arianna Dondi, Sofia Kostoudi, Simon Rosenberg, Véronique Nève, Rumyana Yankova, Barbara Ballmer-Weber, Maria Jose Goikoetxea, María José Barasona Villarejo, Teija Dunder, Tina Vesel, Elisa Gritti, Marianne van Hage, Laure Castan, Etienne Beaudouin, Margherita Di Costanzo, Natalia Ukleja-Sokołowska, Matthew Sperrin, Paul Turner, Saskia Albroscheit, Rebeca Lopez, Sonja Posega Devetak, Francisca Palomares, Laetitia Sellam, Jennifer Hammond, Astrid G. Kruizinga, Konrad Furmanczyk, Patricia Macchiaverni, Olga Pakholchuk, I V Vorozhko, Ania Carsin, Aleksandar Bulog, Joris Mens, Bianca Balbino, James P. Hindley, Jaap H. Akkerdaas, Jonathan Hourihane, Hilde Cnossen, Tatyana A Popova, Rupert Schlags, Natalia Nenasheva, A. Ehrenberg, Sigurveig T. Sigurdardottir, Anna Iliopoulou, Clara Ippolito, Piotr Samel-Kowalik, Paloma Campo, Gador Bogas, Constantinos Pitsios, Sarah Grosche, Karla Leversia Borjas Aguilar, Amena Warner, Birgit Kalb, Lénaïck Dupuis, Harry J. Wichers, Diana Silva, Gabriele Piuri, Linus Grabenhenrich, Emanuel Sarinho, M. Eleonore Pettersson, Rodrigo Barderas, Gary Stiefel, E. N. Clare Mills, Lali Saginadze, Ruta Dubakiene, Francesca Cipriani, Mindy Tsai, Marina Themisb, Michela Ciancamerla, Rebecca Knibb, Ruperto González-Pérez, Marina Peredelskaya, André C. Knulst, Irene Berends, S N Denisova, Montserrat Fernandez-Rivas, Paolo Maria Matricardi, Daniel Sampaio, Giorgio Bedogni, Sira Miquel, Koen Smit, Stefania Arasi, Sylvia Osscini, Justine Courtois, Cristina Deaconu, Srdan Banac, Kirsten Hansen, Jennette Higgs, Charlotte Hands Plovdiv, Carlotta Povesi Dascola, Noe Ontiveros, Rik Schrijvers, Carla Mastrorilli, Maria Passioti, Sebastian Tschirner, Bjorn R. Ludviksson, Joana Caiado, Daniel Corbacho, Sophie Nutten, Agustin Madroñero, Marina Suárez Vergara, Boudewjin J. Kollen, Catherine J Nock, Johanna Rost, Agnieszka Lipiec, Skadi Kull, Geert Houben, Carlo Caffarelli, Jordi Roca-Ferrer, Bolesław Samoliński, Isabelle Cleach, Colette Larré, Aideen Byrne, Corinne Herouet-Guicheney, Jonas Lidholm, Vera Assmann, W. Marty Blom, Adriano Mari, Adam T. Fox, Giuseppe Crisafulli, Afke M. M. Schins, Anastasia Papadopoulou, Flávio Sano, Richard Cooke, Franziska Ruëff, L Jorjoliani, Rebekah Sayers, Anne-Marie Kochuyt, Borja Bartalomé, Anne Moneret-Vautrin, Juan Carlos Julia, Jelena Mihailovic, Katarzyna Pyrz, Kollen Boudewijn, Margherita Varini, Paolo Giordani, Claudia Alessandri, Johanna van der Valk, Antoine Deschildre, Mariam Tskhakaia, Rosina López-Fandiño, Omar Kheroua, Nicolette J. T. Arends, Alina Zbróg, Chung-Hsiung Huang, Andrea Wangorsch, Ulf Bengtsson, Jim Langridge, Dasha Roa-Medellín, Jose Ignacio Larco, Bert Popping, Ana Prieto del Prado, José Antonio Bácter Martos, Rita Nocerino, Sophia Watts, Elisa Haroun, Danilo Villalta, Lee A. Gethings, María Ángeles Algaba Mármol, Ewa Gawrońska-Ukleja, Cornelia Jansen, André Moreira, V A Revyakina, Robert Zacniewski, Xavier Domingo Miró, Francesco Macrì, Mayra de Barros Dorna, Akanksha Sharma, Stephen J. Till, Ali Almontasheri, Chrystyna Kalicinsky, Robbert Sutorius, Akila Rekima, Mark A. Blankestijn, Nicolette Arends, Chiara Pistoletti, Merima Bublin, Manuel Pereira-Barbosa, Reyna Simon, Martin Karjalainen, Sam Mehr, Tushar Banerjee, Carmen Riggioni, Bertrand Evrard, Antonio Carlos Pastorino, Lidia Ilènko, Nikolaos Douladiris, Miguel Vieira, Erik Wambre, Francisco Cabrera-Chavez, Nikos G. Papadopoulos, Piotr Humeniuk, Gert van Duijn, Francesco Zinno, Young-Ae Lee, Lisa Tuppo, Hans de Groot, Léon M.J. Knippels, Pawel Dubiela, Henrik Fomsgaard Kjaer, Andrea Vereda, Djamel Saidi, Kok Loong Ue, Henk Van Loveren, Maria Jose Rodriguez, Marta Lomikovska, Elodie Michaud, Josefina Cernadas, Carmen Ponce, Marysia Recto, Frances Smith, Hassan Al-Dhekri, Shinobu Sakai, Thomas Eiwegger, Ana Rodolfo, Diego Peroni, Nikki Edelbroek, Waltraud Suer, Jenny van Odijk, Irena Nedelea, Borja Bartolomé, Lauren Lissner, Marjeta Sedmak, Annette Jamin, Italo De Vitis, Bo Pontoppidan, Annabelle Capt, Diego G. Peroni, Susana Rodrigues, Juan Carlos López-Rodríguez, David Endesfelder, Lesya Besh, Danila Zennaro, Charlotte G. Mortz, Kati Palosuo, María José Torres, Esozia Arroabarren, Lynne Regent, Laura Valdesoiro Navarrete, Ana Molina, Agha Rehan Khaliq, Phil Couch, Ana Miranda, Thomas Marichal, Riccardo Asero, Denise Borges, Dikla Pivko Levy, Miriam Palacios, Mireille Eb, Stephen J. Galli, Karin Hoffmann-Sommergruber, Anna Gudrun Vidarsdottir, Ifigenia Sfika, André Wolterbeek, Mauro Calvani, Edward F. Knol, Joyce A. M. Emons, Anne-Marie Oomkes-Pilon, Montserrat Bosque García, Daniel Lozano-Ojalvo, Filip Raciborski, Deirdre Galloway, Nanna Juel-Berg, Margareta Brandt Gertmo, Cornelia Bergmayr, Rob Klemans, Juliane Gregersen, Yolanda Meijer, Bettina Brix, Susanne Lau, Karine Adel-Patient, Hanneke van der Kleij, Mareike Price, Jean-Louis Mege, Lizalet Oosthuizen, Agurtzane Bilbao, Indre Butiene, Antonino Romano, Colin Barber, Rosana Camara Agondi, Nour Baïz, Soraya Ainad Tabet, Peter Korošec, Laurian Jongejan, Francine C. van Erp, José Pedro Moreira Silva, Nandinee Patel, Jaime Lozano, Prescilla V. Jeurink, Artur Walkiewicz, Bryan M. Harvey, Tiina J. Kauppila, Aida Semic-Jusufagic, Marianna Murdjeva, Miren Arteaga, Y. Bouferkas, Geunwoong Noh, Henny G. Otten, Sabine Dölle, Christopher Munro, O Dominguez, Gerard H. Koppelman, Leonieke N. van Veen, Vasti Iancu, Georg Mitterer, Patrizia Polverino de Laureto, Juliane Schulz, Ewa Ternesten Hasseus, Caroline Zimmermann, Ivona Barcievic-Jones, Shira Benor, Susanne Schwarz, Eun Ha Jang, Josefina Rodrigues Cernadas, Tadej Avcin, Joseph L. Baumert, Pernille Winther, Stéphane Leteurtre, Gabriela Canto, Louise J Michaelis, Jorge Alvarez, Gabriel Gastaminza, Michela Carola Speciani, Rute Gonçalves, Leire Dopazo, Evgen Benedik, Susanne C. Diesner, Luc S. De Clerck, A. Wesley Burks, Maurits S. van Maaren, Salvatore Tripodi, Philippe Aubert, Kamel Eddine El Mecherfi, Aline B. Sprikkelman, Ana Prieto, Margitta Worm, Edyta Krzych, Maja Krstic, Iride Dello Iacono, Melanie Cap, Alf Weimann, Maria Nassiri, Niels Röckendorf, Vladyslava Barzylovych, Marcia C. Mallozi, Pierre Bruhns, Jeanette Fisker Trandbohus, Ekaterini Papadopoulou, Vicente Albendiz, Timothy Watts, Uta Jappe, Javier Moreno, Maria Carmen Verga, Beatriz Secades Barbado, Carmen Di Scala, Roy Gerth van Wijk, Vladimir Mićović, Esther Barrionuevo, Giampaolo Ricci, Luisa Galindo, Ana Paula Beltran Moschione Castro, Oona Mustonen, Jia Yin, Lucia Caminiti, Jorge Esparza-Gordillo, N Adamia, Anna-Maija Hanni, Romy Gadisseur, Stefano Luccioli, Regina Treudler, Rosetta Ferrara, M. Guendouz, Jaakko Yrjänä, Philipp Starkl, Premendra D. Dwivedi, Javier Cuesta-Herranz, Johan Garssen, Ekaterini Goudouris, Sridevi Muralidharan, Kate Grimshaw, Carolina Sanchez Aranda, Ioana Maris, Manzoor Ahmed, Hajime Karasuyama, Stephanie Claus, Chantal Agabriel, Karen English, Dorien Van Ginkle, Eleonora Savi, Loredana Chini, Ine I. Decuyper, Sabine Schnadt, Valérie Trendelenburg, Jean-Luc Fauquert, Maurizio Mennini, Nikolaos Mikos, Ana Célia Costa, Steve L. Taylor, A. A. Schoemaker, Sara Abián, Margo M. Hagendorens, Andrea Di Rienzo Businco, Melina Makatsori, Eugénia Matos, Lucy Walker, Nikolaos A. Kitsioulis, David Alejandro Mendoza Hernández, Maria Starkhammar, Djordje Filipovic, Aine Adams, Mukul Das, Sonsoles Intente-Herrero, Natalia Blanca López, Marek L. Kowalski, Diana Deleanu, Bernard P. Mahon, Jean-Michel Wal, Lucia Decastelli, Mihaela Popescu, Aimee Lou Nano, Eva Batanero, Tong-Rong Jan, Yolanda Puente, Jacek Borowicz, Aimée Dorkenoo, J. Östling, Mashary Altamimi, Michel Neunlist, Zerrin Yalvaç, Sonia Ricò, Wentong Xue, Linda Cosenza, David C. A. Candy, Robert D. Voyksner, Montserrat De Prada, Abdulhadi Al-Qahtani, Sébastien Holvoet, Wolf-Meinhard Becker, Meropi D. Kontogianni, Bruno Pereira, F. Pineau, Eva Corbet, Kirsten Mehlig, Rosella De Poi, Jolanda H. M. van Bilsen, José Luís Plácido, Hans-Jørgen Malling, Chia-Chi Wang, Philip Couch, Kerrie Kirk, Agata Michalska, Sylke Rietz, Mariya Ivanovska, Victor Matheu-Delgado, Carl Hamsten, Francisca Gómez, Neusa Falbo Wandalsen, Mika J. Mäkelä, Tatyana Sentsova, Kristian Bravin, Philippe Delahaut, Hervé Bernard, Leonor Carneiro-Leão, Michele Miraglia del Giudice, Elena D. Kuvshinova, Jochen Behrends, Caroline Klingebiel, Meta Accetto, Claire Mills, Mariona Pascal, Miguel García Domínguez, Huan Rao, Carmen Saviana Ganea, Umberto Pelosi, Stefano Pattini, Bodo Niggemann, Annamaria Bianchi, Laura Martín-Pedraza, Anna Selby, Cristina Bueno, Stefan Vieths, Luis Felipe Ensina, Florin-Dan Popescu, Antonio Fernandez, Soren Wille, Erna Van Hoeyveld, Carina Kelleher, Athina L. Van Gasse, Anders Blom Jensen, Zorica Zivkovic, Moshe Ben-Shoshan, Sara Pereiro Fernández, Ronald van Ree, Antima Banerjee, Pablo Merida, Mandy Ziegert, Barbara Wróblewska, Morten Christensen, Gador Gomez, Pablo San Segundo-Acosta, Khaled Messaoudi, Anne-Sofie Ravn Ballegaard, Mikael Kuitunen, David Luyt, Vito Sabato, Nesrine Zaabat, Svitlana Zubchenko, Julien Labreuche, Elin Lustig, Katharina Blumchen, Guillaume Pouessel, Ana Fiandor, Stéphanie Lejeune, Paola Dignetti, Helen Brown, Anastasia Cirisano, Evangelia Kompoti, Anna Sokolova, Mercedes Escarrer Jaume, Shan Deng, Dirceu Solé, Chiara Fiamingo, Alessandro Travaglini, Nicolas Gaudenzio, Zbigniew Bartuzi, Antonia Rojas, Roberta Olcese, Nanju Alice Lee, Sandra Brandhoff, Lucien F. Harthoorn, Anna Maria Szyc, Andrea Costanzi, Sabine Pfeifer, Emiliano De Dominicis, Chiara Rafaiani, Yin-Hua Cheng, Elida Nikolla, Ignacio García Núñez, Diego Faggian, Lieve Coorevits, Ayelet Rimon, Erika Jensen-Jarolim, Jacqueline J.M. Castenmiller, Kristin Verbeke, Sean Bennett, Paraskevi Xepapadaki, Glauce Hiromi Yonamine, Anna Wawrzeńczyk, Rusudan Karseladze, Emmanouil Manousakis, Fiona Ward, Ivana Filipovic, Marie Bodinier, Viviana Moschese, Pedro Giavina Bianchi, Alice Coimbra, Alena Berger, Anton A. Shekhetov, Monica Maćków, Philippe Egenmann, Oscar Asensio, Lidia Hanna Markiewicz, Chun-Wei Tung, Zsolt Szépfalusi, Jan Knol, Frits Koning, Carolina S. Aranda, Annick Bastiaensen, Giovanni Battista Pajno, Leticia Pérez-Rodríguez, Ewout W. Steyerberg, Riccardo Sibilano, Valérie Verhasselt, Roberta Lupi, Lukasz Sokolowski, Adam Wawrzeńczyk, Dimitris I. Mitsias, Andreia Forno, Antoine Magnan, Christian Schwager, Ioanna Manolaraki, Alessandro Fiocchi, Tanja Rouhani Rankouhi, Carla Jones, Ana Pereira, Hannah M. Kansen, Michael Clausen, D. Bignardi, Assad M. Butt, Julie C. Locklear, Katrine Lindholm Bøgh, Valery Muhortnich, María Teresa Giner, Juan Miguel Garcia, Maria Luisa Somoza, Raditsa Sokolova, Maria Pasioti, Mirjana Zupancic, Joana Vitte, Duncan Brown, Arnaldo Porto, M. Turfkruyer, Lau Fabricius Larsen, Filipe Benito-Garcia, Mayra Coutinho Andrade, Meltem Ugras, Ingrid Sutic, Rand K. Arnaout, Etienne Cavalier, Marta Neto, Grégory Bouchaud, Elena Varin, Bushra Javed, Carla Camerotto, Monica Bronkowska, Cristiano Caruso, Luís Amaral, Jackeline F. Motta, Sahar Elshorbagi, Cornelis K. van der Ent, Alexandra Rodrigues, Silvia Peveri, Juan Heber Castellanos, Muriel Totis, Joan Bartra, Gjustina Loloci, Ivana Giangrieco, Ekaterina Khaleva, Joaquín Navarro, Kayoko Matsunaga, Jlenia Fresta, Jonathan O'b Hourihane, Sabina Bijlsma, Ana Rodriguez-Fernandez, Rosita Aitoro, Daniela Manila Bianchi, Rosa Jimenez-Feijoo, Mario Plebani, Marleen T. J. Van Ampting, Anthony E.J. Dubois, Clémence Mordacq, Simone Frediani, Martin Chapman, Helena Larramona, Fabrícia Carolino, Yanne Boloh, Ivona Baricevic-Jones, Mathilde Claude, Caroline Thumerelle, Päivi Vähäsarja, Mareen R. Datema, Abdullah Alfhaid, Diana Perez Alzate, Laura Santos-Diez, Graham King, Maria Teresa Guerra Perez, Jean-Marie Renaudin, Frieke Kuper, Josué Alejandro Huertas Guzmán, Harry Wichers, Thomas Keil, A. Haddi, Jennifer Santos, Regina Selb, Aida del Campo García, Maria Basagaña, Valentino Pavišić, Angela Simpson, Chris H. Bridts, Susana Piedade, Silvia Gallina, Isabella Pali-Schöll, Inês Pádua, Margaretha A. Faber, Frédéric de Blay, Luís Câmara Pestana, Ben Remington, Anna S. Pelkonen, Sandra Lucarelli, Ivana Šutić, Olivier Tranquet, Montserrat López Onieva, Antonio Amoroso, Paola Minale, Katia Basello, George Du Toit, Daniela Adriano, Adnan Custovic, Zbikowska-Gotz M, Domingo Barber, Inmaculada Doña, Christine Breynaert, Hanan Sharif, Manon M. Oude Nijhuis, Sandra Denery, Bertine M. J. Flokstra-de Blok, Rinkesh Kumar Gupta, Pieter-Jan de Kam, Dianne E. Campbell, Carmen M. D'Amelio, Nunzia Maiello, Ingo Marenholz, José María Ignacio García, Helen Lindqvist, Lilian Moraes, Cleonir Lui de Moraes Beck, Eunice Dias de Castro, Cono Casale, Barbara Majkowska-Wojciechowska, Maria Petrodimopoulou, Andrea Mikkelsen, Hub P. J. M. Noteborn, Hulya Ercan Saricoban, Moira Austin, Martinus Løvik, Graham Roberts, Isabella Annesi-Maesano, Aeilko H. Zwinderman, Sigrun H. Lund, Anouska D. Michelsen-Huisman, Fernando Bandrés Sánchez-Cruz, Francisco Javier Ruano, Valérie Liabeuf, Eman Madbouly, Pasquale Comberiati, Maria Isabel Garcimartin, Stephan Scheurer, Inna A. Larkova, Jean Tratt, Renata Rodrigues Cocco, Younes Belabbas, Lorella Paparo, Elizabeth Griffiths, Gian Lodovico Rapaccini, Audrey Dunn Galvin, Zizi Cojocariu, Isidor Hutteger, Claire Claverie, S.M. Nedelska, Anna Kuklinska-Pijanka, Jenny Badas, Mónica Piquer, Iztok Devetak, A.K.F. Gushken, Frans Timmermans, Adriana Machinena, Francisco Javier Monteseirin, Amaranta Traversa, Fátima Cabral Duarte, Tomaž Poredoš, Peter Meyer, Cristina Arêde, Rosa Muñoz-Cano, Barbara Pfitzner, Alejandro Joral, Juan Carlos Daza, Laurent L. Reber, Olga Chernyak, and Maria Antonetta Ciardiello

Subjects
Pulmonary and Respiratory Medicine, Allergy, medicine.medical_specialty, 030504 nursing, business.industry, Immunology, RC581-607, medicine.disease, Meeting Abstracts, 03 medical and health sciences, 0302 clinical medicine, Food allergy, 030225 pediatrics, Family medicine, medicine, Immunology and Allergy, Immunologic diseases. Allergy, 0305 other medical science, business, Anaphylaxis
Published
2017

6. Immunoglobulin-E-binding epitopes of wheat allergens in patients with food allergy to wheat and in mice experimentally sensitized to wheat proteins

Author

K. Adel-Patient, Didier Marion, Bénédicte Bakan, Denise-Anne Moneret-Vautrin, Sandra Denery-Papini, F. Pineau, Marie Bodinier, D. Kasarda, T. Mothes, Hamza Mameri, S. Triballeau, and Olivier Tranquet

Subjects
0303 health sciences, Allergy, biology, Immunology, food and beverages, medicine.disease, Immunoglobulin E, medicine.disease_cause, Epitope, 03 medical and health sciences, 0302 clinical medicine, Epitope mapping, 030228 respiratory system, Antigen, Pepscan, Food allergy, Allergic response, medicine, biology.protein, Immunology and Allergy, 030304 developmental biology
Abstract

Summary Background At present, B cell epitopes involved in food allergy to wheat are known only for a few allergens and a few categories of patients. Objective To characterize the epitopes of different wheat kernel allergens: α-, γ, ω2, and ω5-gliadin, a low-molecular-weight (LMW) glutenin subunit, and a lipid transfer protein (LTP1) recognized by allergic patients and by sensitized mice and provide further understanding of the role of structure in determining allergic response. Methods Sera were obtained from 39 patients suffering from food allergy to wheat. BALB/c mice were sensitized to gliadins or LTP1 by intraperitoneal immunizations. Continuous epitopes bound by IgE were delineated by the Pepscan technique. The response to reduced, alkylated LTP1 was compared with that of the native form to evaluate the importance of protein folding on IgE reactivity. Results Few continuous epitopes of LTP1 reacted with IgE from allergic patients and mice, but one of them was common to several patients and sensitized mice. The unfolded protein was not recognized by either patient or mouse IgE, emphasizing the major role of LTP1 folding and discontinuous epitopes in IgE-binding. In contrast, many continuous epitopes were detected by patient and mouse IgE especially for an ω5-gliadin, which is an unstructured protein, and to a lesser extent, for the other gliadins and a LMW-glutenin subunit. Conclusion and Clinical Relevance The conformation of LTP1 appeared to have a strong impact on the type of IgE-binding epitopes elicited by this protein in both man and mouse. The responses in mice sensitized to gliadins or LTP1 were sufficiently comparable with the human response in terms of IgE-binding epitopes to provide support for the use of the mouse model in further investigations. Cite this as: S. Denery-Papini, M. Bodinier, F. Pineau, S. Triballeau, O. Tranquet, K. Adel-Patient, D.A. Moneret-Vautrin, B. Bakan, D. Marion, T. Mothes, H. Mameri and D. Kasarda, Clinical & Experimental Allergy, 2011 (41) 1478–1492.

Published
2011

7. Contents Vol. 146, 2008

Author

Hsiao-Yun Tai, Chi-Huei Chiang, D.A. Moneret-Vautrin, Timo Buhl, Dae Yong Kim, Marie Bodinier, S. Triballeau, Leopoldo Aguilera-Aguirre, Christiane Kruse Fæste, Martine Morisset, Maud Hrabina, Philippe Moingeon, Gaynour B.G. Sletten, Helene Lindvik, Véronique Bordas, Jai Youl Ro, Istvan Boldogh, P. de Coppet, Karl-Christian Bergmann, Attila Bacsi, F. Pineau, Alfredo Saavedra-Molina, Gabriel Peltre, Ulrich Blank, Horng-Der Shen, Thomas Fuchs, Maaike M.B.W. Dooper, Hong Chou, Lise Holden, Chantal Brossard, Ming F. Tam, Lin-Hau Lee, Marian L. Kruzel, Franco Frati, Siegfried Jaeger, Ji Young Kim, Basil O. Ibe, Barun K. Choudhury, J. Usha Raj, Hubert Kampmann, Raphael C. Panzani, May F. Abdallah, Sandra Denery-Papini, Jose Martinez, and Claudine Guérin-Marchand

Subjects
business.industry, Immunology, Immunology and Allergy, Medicine, General Medicine, business
Published
2008

8. Assessment of the Allergenic Potential of Transgenic Wheat (Triticum aestivum) with Reduced Levels of ω5-Gliadins, the Major Sensitizing Allergen in Wheat-Dependent Exercise-Induced Anaphylaxis

Author

Roberta Lupi, Charlene K. Tanaka, Martine Drouet, Susan B. Altenbach, Sandra Denery-Papini, F. Pineau, Martine Morisset, Etienne Beaudouin, USDA-ARS : Agricultural Research Service, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Unité d'Allergologie Générale et de Pneumologie, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Centre hospitalier d'épinal, and Centre Hospitalier Luxembourg

Subjects
Adult, Male, Glutens, Transgene, Flour, Food, Genetically Modified, Triticum aestivum, immunoblots, Wheat Hypersensitivity, Biology, Immunoglobulin E, medicine.disease_cause, digestive system, gliadins, Gliadin, gene silencing, Allergen, Glutenin, RNA interference, Food allergy, wheat, medicine, Humans, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Anaphylaxis, Exercise, Triticum, WDEIA, 2. Zero hunger, food allergy, business.industry, nutritional and metabolic diseases, food and beverages, General Chemistry, Antigens, Plant, medicine.disease, Plants, Genetically Modified, digestive system diseases, Biotechnology, Immunology, biology.protein, Female, General Agricultural and Biological Sciences, business, gluten proteins
Abstract

International audience; The omega 5-gliadins are the major sensitizing allergens in wheat-dependent exercise-induced anaphylaxis (WDEIA). In this study, two-dimensional immunoblot analysis was used to assess the allergenic potential of two transgenic wheat lines in which omega 5-gliadin genes were silenced by RNA interference. Sera from 7 of 11 WDEIA patients showed greatly reduced levels of immunoglobulin E (IgE) reactivity to omega 5-gliadins in both transgenic lines. However, these sera also showed low levels of reactivity to other gluten proteins. Sera from three patients showed the greatest reactivity to proteins other than omega 5-gliadins, either high-molecular-weight glutenin subunits (HMW-GSs), alpha-gliadins, or non-gluten proteins. The complexity of immunological responses among these patients suggests that flour from the transgenic lines would not be suitable for individuals already diagnosed with WDEIA. However, the introduction of wheat lacking omega 5-gliadins could reduce the number of people sensitized to these proteins and thereby decrease the overall incidence of this serious food allergy.

Published
2015

9. What is the impact of genetic transformation on wheat allergenicity?

Author

F. Pineau, Stefania Masci, Denise-Anne Moneret-Vautrin, Olivier Tranquet, Roberta Lupi, Colette Larré, Sandra Denery-Papini, G. Deshayes, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Tuscia University, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), The European Academy of Allergy and Clinical Immunology (EAACI). CHE., and ProdInra, Migration

Subjects
Pulmonary and Respiratory Medicine, Allergy, [SDV.IMM] Life Sciences [q-bio]/Immunology, Globulin, Immunology, Crop, 03 medical and health sciences, 0302 clinical medicine, Food allergy, Genotype, medicine, Immunology and Allergy, Ingestion, 030212 general & internal medicine, Food science, Cultivar, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, biology, business.industry, food and beverages, medicine.disease, Biotechnology, 030228 respiratory system, Poster Presentation, biology.protein, [SDV.IMM]Life Sciences [q-bio]/Immunology, business, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, Wheat allergy
Abstract

Wheat allergy occurs by inhalation (baker's asthma) and ingestion (food allergy), but may also develop by contact in some cases. The responsible allergens of wheat are proteins accounting for about 10-15% of the grain dry weight. Wheat proteins are divided into two groups: the salt soluble fraction (albumins/globulins) and the gluten proteins (gliadins and glutenins). This latter group is responsible for celiac disease and also for food allergy, whereas the albumins/globulins are involved in baker's asthma and in some food allergy. Genetic modification (GM) technology for crop improvement has recently emerged and its impact on allergenicity must be evaluated, as recommended by the Codex Alimentarius. Following this recommendation, we first compared two GM lines with their parents using an allergenomic approach then in a second step we aimed at comparing the amount of allergenic polypeptides in these GM wheats, their untransformed genotypes and those measured among twenty commercial cultivars, either durum or bread wheats. In order to characterize the accumulation of allergenic proteins in wheats, sera from children and adults with clinically documented wheat allergy were used. The investigation is focused mainly on the soluble protein fraction of wheat. For the comparison of GM lines with their natural counterparts, 2D immunoblot followed by mass spectrometry analysis and protein identification was set up. ELISA tests were performed on the whole set of genotypes. Few differences at molecular and quantitative levels were revealed between the GM lines and their counterparts. Two new IgE-binding proteins were detected for one GM line.[1] We also observed that the genetic transformation may impact IgE reactivity, either in a positive or in a negative way.[2] This study leads us to conclude that a wide variation exists in the amount of allergenic polypeptides among durum and bread wheat cultivars, and that the differences observed between GM wheats and their parents are within the range of these 20 cultivated wheats.

Published
2015

10. Food allergy to wheat: identification of immunogloglin E and immunoglobulin G-binding proteins with sequential extracts and purified proteins from wheat flour

Author

F. Battais, L. Guerin, F. Pineau, Gisèle Kanny, Sandra Denery-Papini, Denise-Anne Moneret-Vautrin, C. Aparicio, and Yves Popineau

Subjects
Globulin, Immunology, Immunoglobulin E, Immunoglobulin G, 03 medical and health sciences, 0404 agricultural biotechnology, 0302 clinical medicine, Glutenin, Food allergy, medicine, Immunology and Allergy, 2. Zero hunger, biology, medicine.diagnostic_test, Chemistry, Radioallergosorbent test, Albumin, food and beverages, 04 agricultural and veterinary sciences, medicine.disease, 040401 food science, 3. Good health, 030228 respiratory system, Biochemistry, IgG binding, biology.protein
Abstract

BACKGROUND: Cereal-associated allergy is particularly considered a serious problem, because cereals are essential in our daily diet. Wheat proteins are classified into albumins, globulins and prolamins (insoluble gliadins and glutenins). OBJECTIVES: Our objectives were to study the involvement in food allergy to wheat of these different protein types by using purified fractions and to identify those binding IgE and IgG antibodies. METHODS: Sera were obtained from 28 patients with food allergy to wheat. Albumins/globulins, gliadins and glutenins were obtained by sequential extraction based on differential solubility; alpha-, beta-, gamma- and omega-gliadins and low molecular weight (LMW) and high molecular weight (HMW) glutenin subunits were purified by chromatography. IgE binding to these extracts and fractions were analysed by radioallergosorbent test (RAST), and immunoblotting; IgG binding was detected by enzyme-linked immunosorbent assay (ELISA). RESULTS: In RAST, 60% of sera were shown to have specific IgE antibodies against alpha-, beta-gliadins and LMW glutenin subunits, 55% to gamma-gliadins, 48% to omega-gliadins and 26% to HMW glutenins. Immunoblotting analysis confirmed results obtained in RAST concerning LMW and HMW glutenin subunits and showed that 67% of patients have IgE antibodies to the albumin/globulin fraction. CONCLUSION: Results obtained in the different tests showed common features and in agreement with other studies indicated the presence of numerous allergens in food allergy to wheat; alpha-, beta-, gamma- and omega-gliadins, LMW glutenin subunits and some water/salt-soluble proteins appeared as major IgE binding allergens, whereas HMW glutenins were only minor allergens. The same type of antigenic profile against gliadins and glutenins was observed with IgG antibodies. Important sequence or structural homologies between the various gliadins and LMW glutenin subunits could certainly explain similarity of IgE binding to these proteins.

Published
2003

11. The mothif study. Costs of Clotting Factors In Haemophilia A And B: A Review From The Berhlingo Database In Seven Comprehensive Health Care Centres In France

Author

V Horvais, M Pennetier, G Chapelle, A Athouel, S Bauer, V Cogulet, C Lebert, M Clerc, I Vincent, Y Poirier, N Le Gall, V Pacaud, S Daubie, S Orhon-Ménard, M Gaumé, P Jaccard, M Haudot-Lépine, J Teil, J Huaut-Quentel, B Lassère, M Kuzzay, M Roy, A Gaudy, C Baubri, A Courtois, I Baudin Jacquemin-Aubry, N Ripoll, P Chauvet, B Pan-Petesch, L Ardillon, B Guillet, P Beurrier, F Pineau-Vincent, L Macchi, L Ferrand, M Trossaërt, and null BERHLINGO Group

Subjects
Clotting factor, business.industry, Health Policy, Health care, Haemophilia A, Public Health, Environmental and Occupational Health, Medicine, Medical emergency, business, medicine.disease
Published
2016

12. Identification of allergens and epitopes involved in allergy to deamidated gluten

Author

Colette Larré, Marie Bodinier, Chantal Brossard, F. Pineau, Denise-Anne Moneret-Vautrin, Manon Pietri, Evelyne Paty, Sandra Denery, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Centre hospitalier Jean Monnet, Service de Pneumologie et d'Allergologie Pédiatriques, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and The European Academy of Allergy and Clinical Immunology (EAACI). Zurich, CHE.

Subjects
Pulmonary and Respiratory Medicine, désamidation, Allergy, ble tendre, Immunology, Ingénierie des aliments, Immunoglobulin E, medicine.disease_cause, méthode de traitement, Epitope, allergens, deamidation, epitope, gliadins, wheat, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Allergen, allergène alimentaire, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Immunology and Allergy, Medicine, Food engineering, Deamidation, gliadine, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, biology, business.industry, food and beverages, épitope allergénique, gluten de blé, medicine.disease, Gluten, 3. Good health, critère d'identification, chemistry, Pepscan, biology.protein, Oral Presentation, business, Wheat allergy
Abstract

Background Gluten proteins can be modified by deamidation to enhance their solubility and technological applications. However severe allergic reactions have been reported after the consumption of food products containing deamidated gluten in subjects tolerant to wheat. This work aimed to characterize allergen profiles for these patients in comparison with those of patients allergic to wheat and identify IgE-binding epitopes. Methods Sera were obtained from 15 patients allergic to deamidated gluten (DG) and from 9 patients allergic to wheat proteins (WP). IgE-binding profiles were characterized both in ELISA and in a humanized rat basophilic leukemia (RBL) cell model. Epitopes were mapped on g and ω2-gliadin sequences by Pepscan and effect of glutamine/glutamic acid substitutions were studied. Results Compared to the heterogeneous pattern of allergens detected by IgE from patients allergic to WP, responses of patients allergic to DG were homogeneous. In ELISA, all the sera displayed IgE-binding to deamidated g and ω2-gliadins and deamidated total gliadins, frequently with high concentrations. These modified proteins induced RBL degranulation with most of the sera from DG-allergic patients. A consensus epitope was found on native g and ω2-gliadins (QPQQPFPQ); it was repeated several times in their sequences. The substitution of two or three glutamines of this epitope into glutamic acid at positions Q3 or Q4 and Q8 (QPEEPFPE) increased its recognition the best. Conclusion Allergy to DG is a separate entity from wheat allergy characterized by a homogeneous IgE response. Deamidated ω2-gliadins or the dominant IgE-binding epitope QPEEPFPE could be used as tools for the diagnosis of this new allergy. Disclosure of interest None declared.

Published
2013

13. Allergy to deamidated gluten in patients tolerant to wheat: specific epitopes linked to deamidation

Author

Manon Pietri, F. Battais, Marie Bodinier, Colette Larré, Chantal Brossard, F. Pineau, S. Triballeau, Denise-Anne Moneret-Vautrin, Evelyne Paty, Sandra Denery-Papini, T. Mothes, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Département Polluants et Santé, Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), Institute for Laboratory Medicine, Otto Wagner Hospital Vienna, Service de Pneumologie et d'Allergologie Pédiatrique, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Lorraine (UL), and Centre hospitalier Jean Monnet

Subjects
Male, Wheat Hypersensitivity, medicine.disease_cause, Immunoglobulin E, Epitope, Gliadin, gliadins, Epitopes, 0302 clinical medicine, Allergen, wheat, Immunology and Allergy, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Deamidation, Child, Triticum, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, biology, food and beverages, Middle Aged, Basophils, deamidation, Biochemistry, Child, Preschool, Female, Protein Binding, Adult, Adolescent, Glutens, Immunology, Basophil Degranulation Test, Cell Line, 03 medical and health sciences, Young Adult, Food allergy, medicine, Animals, Humans, Amino Acid Sequence, 030304 developmental biology, food allergy, Infant, Allergens, medicine.disease, Gluten, Rats, IgE-binding epitopes, 030228 respiratory system, chemistry, Pepscan, biology.protein, Wheat allergy
Abstract

Background Gluten proteins can be modified by deamidation to enhance their solubility and technological applications. However, severe allergic reactions have been reported after the consumption of food products containing deamidated gluten (DG) in subjects tolerant to wheat. This work aimed to characterize allergen profiles for these patients in comparison with those of patients allergic to wheat and to identify IgE-binding epitopes. Methods Sera were obtained from 15 patients allergic to DG and from nine patients allergic to wheat proteins (WP). IgE-binding profiles were characterized both in ELISA and in a humanized rat basophilic leukaemia (RBL) cell model. Epitopes were mapped on γ- and ω2-gliadin sequences by Pepscan, and effect of glutamine/glutamic acid substitutions was studied. Results Compared to the heterogeneous pattern of allergens detected by IgE from patients allergic to WP, responses of patients allergic to DG were homogeneous. In ELISA, all the sera displayed IgE binding to deamidated γ- and ω2-gliadins and deamidated total gliadins, frequently with high concentrations. These modified proteins induced RBL degranulation with most of the sera from DG-allergic patients. A consensus epitope was found on native γ- and ω2-gliadins (QPQQPFPQ); it was repeated several times in their sequences. The substitution of two or three glutamines of this epitope into glutamic acid at positions Q3 or Q4 and Q8 (QPEEPFPE) increased its recognition the best. Conclusion Allergy to DG is a separate entity from wheat allergy. It can be evidenced by strong IgE binding to deamidated gliadins or peptides of the type QPEEPFPE.

Published
2012

14. Evaluation of an in vitro mast cell degranulation test in the context of food allergy to wheat

Author

Claudine Guérin-Marchand, Marie Bodinier, Chantal Brossard, S. Triballeau, Sandra Denery-Papini, P. de Coppet, D.A. Moneret-Vautrin, F. Pineau, Ulrich Blank, Martine Morisset, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie des Adaptations Nutritionnelles (PhAN), and Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)

Subjects
Urticaria, Wheat Hypersensitivity, WHEAT ALLERGY, Immunoglobulin E, FC EPSILON RI, Cell Degranulation, Gliadin, TEST DE DEGRANULATION, 0302 clinical medicine, [SDV.IDA]Life Sciences [q-bio]/Food engineering, HUMANIZED RAT CELL LINE, Immunology and Allergy, Fluorometry, DERMATITE ATOMIQUE, ANAPHYLAXIE A L'EFFORT, 2. Zero hunger, 0303 health sciences, biology, Reverse Transcriptase Polymerase Chain Reaction, ALBUMIN/GLOBULIN FRACTION, food and beverages, General Medicine, Middle Aged, Mast cell, Flow Cytometry, medicine.anatomical_structure, ELISA, Adult, Globulin, Immunology, Context (language use), Enzyme-Linked Immunosorbent Assay, Transfection, Cell Line, Dermatitis, Atopic, 03 medical and health sciences, OMEGA 5-GLIADINS, Food allergy, medicine, Animals, Humans, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, RNA, Messenger, IGE, 030304 developmental biology, Receptors, IgE, MAST CELLS, Albumin, Infant, medicine.disease, Rats, RBL-2H3, biology.protein, Wheat allergy, 030215 immunology
Abstract

Background: Antigenic profiles obtained by ELISA with IgE from patients with wheat food allergy (WFA) established that major allergens are albumins/globulins (AG) for children suffering from atopic eczema/dermatitis syndrome (AEDS), ω5-gliadins for adults suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA), anaphylaxis or urticaria and low-molecular-weight (LMW) glutenin subunits for patients with anaphylaxis. We aimed to characterize a new mast cell transfectant for its ability to degranulate with wheat proteins and patient sera and compare these results to those obtained by ELISA. Methods: Thirty sera from patients with WFA were tested: 14 with AEDS (group 1) and 16 with WDEIA, anaphylaxis or urticaria (group 2). An IgE Fc receptor (FcΕRI) humanized rat RBL-2H3 line was established by transfection with cDNAs encoding α-, β- and γ-subunits for the human IgE receptor. Results: A humanized RBL clone was selected for its capacity to express mRNA α-, β- and γ-subunits of FcΕRI, to bind allergen-specific human IgE and to degranulate. In group 1, sera induced enhanced degranulation with AG extract, but rarely reacted with gliadins and glutenins. In group 2, half of the sera showed degranulation with LMW glutenins whereas the AG fraction and lipid transfer proteins were rarely positive. ω5-Gliadins did not appear as a major allergen in degranulation assays, although functional allergen-specific IgE was measurable in appreciable amounts. Conclusion: Our data demonstrate that in wheat food allergen evaluation, correlation exists between mast cell degranulation and IgE measurements, depending on the type of allergen. Therefore, the biological activity of some allergen types may also be affected by other parameters.

Published
2008

15. Influence of the allelic variants encoded at the Gli-B1 locus, responsible for a major allergen of wheat, on IgE reactivity for patients suffering from food allergy to wheat

Author

Yves Popineau, Michel Laurière, Martine Morisset, Catherine Pecquet, Gérard Branlard, Dominique Choudat, Marie Bodinier, Elodie Boulenc, Isabelle Bouchez-Mahiout, Marielle Merlino, Sandra Denery-Papini, Denise-Anne Moneret-Vautrin, F. Pineau, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Chimie Biologique (UCB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Service de Médecine Interne, Immunologie Clinique et Allergologie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Laboratoire de Médecine et Thérapeutique Moléculaire, CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Faculté de Médecine, AP-HP, Assistance Publique - Hôpitaux de Paris, Service de Pathologie Professionnelle, Université Paris Descartes - Paris 5 (UPD5), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA), Hôpital Tenon, Centre d'allergologie, Assistance Publique - Hôpitaux de Paris, Institut National de la Recherche Agronomique (INRA) - AgroParisTech, Institut National de la Recherche Agronomique (INRA) - Université Blaise Pascal - Clermont-Ferrand 2 (UBP), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
0106 biological sciences, Urticaria, IMMUNOGLOBINE E, Immunoglobulin E, medicine.disease_cause, 01 natural sciences, FOOD ALLERGY, Gliadin, OMEGA(5)-GLIADINS, Allergen, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Triticum, 2. Zero hunger, 0303 health sciences, ALLELIC VARIANTS, food and beverages, [SDV.IDA] Life Sciences [q-bio]/Food engineering, Middle Aged, Plant protein, Rabbits, General Agricultural and Biological Sciences, ANAPHYLAXIE, Food Hypersensitivity, Adult, WHEAT, Locus (genetics), Biology, 03 medical and health sciences, Food allergy, medicine, Animals, Humans, Genetic variability, EXERCISE INDUCED ANAPHYLAXIS, IGE, Allele, Anaphylaxis, Exercise, Alleles, Aged, 030304 developmental biology, Immune Sera, fungi, Genetic Variation, nutritional and metabolic diseases, General Chemistry, Allergens, Antigens, Plant, medicine.disease, digestive system diseases, Immunology, biology.protein, 010606 plant biology & botany
Abstract

Wheat presents an important genetic diversity that could be useful to look for cultivars with reduced allergencity. omega5-Gliadins have been described as major allergens for wheat allergic patients suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA) and some cases of chronic urticaria (U). Our objective was to study the influence of genetic variability at the Gli-B1 locus encoding for omega5-gliadins on the reactivity of IgE antibodies from these patients. We selected cultivars expressing 13 alleles at Gli-B1 including a wheat/rye translocation and studied the reactivity to gliadins of a rabbit antiserum specific for omega5-gliadins and of IgE from 10 patients. The antiserum and IgE from nine patients with WDEIA and U strongly detected omega5-gliadins expressed by most of the Gli-B1 alleles but showed no or faint responses to the gliadins and secalins extracted from the translocated wheat. The selection of genotypes lacking the Gli-B1 locus may reduce wheat allergenicity.

Published
2007

16. Identification of IgE-binding epitopes on gliadins for patients with food allergy to wheat

Author

F. Pineau, Sandra Denery-Papini, T. Mothes, Denise-Anne Moneret-Vautrin, F. Battais, G. Kanny, Yves Popineau, Marie Bodinier, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), and Laboratoire LTEE

Subjects
Adult, Allergy, Immunology, Molecular Sequence Data, Wheat Hypersensitivity, Immunoglobulin E, AEDS, sequential B-epitopes, Coeliac disease, Epitope, Gliadin, gliadins, Dermatitis, Atopic, 03 medical and health sciences, 0302 clinical medicine, Food allergy, medicine, Immunology and Allergy, Humans, Amino Acid Sequence, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Anaphylaxis, Exercise, Triticum, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, WDEIA, 0303 health sciences, biology, business.industry, Immunodominant Epitopes, nutritional and metabolic diseases, food and beverages, Allergens, medicine.disease, digestive system diseases, pepscan analysis, wheat allergy, 030228 respiratory system, Pepscan, Child, Preschool, biology.protein, business, Sequence Alignment, Wheat allergy
Abstract

BACKGROUND Food allergy to wheat induces different symptoms as atopic eczema/dermatitis syndrome (AEDS), urticaria and more severe reactions as wheat-dependent exercise-induced anaphylaxis (WDEIA). Different gliadin classes are involved in this allergy but IgE-binding epitopes are known only on omega5-gliadins and for WDEIA cases. OBJECTIVES The aim of the study was to identify IgE-binding epitopes on several gliadin classes and for several patients with different symptoms and ages. METHODS Eleven sera were analysed by pepscan with overlapping synthetic peptides. RESULTS Sera from five patients with anaphylaxis, urticaria or WDEIA, displayed strong IgE-binding to sequential epitopes of the repetitive domains of alphabeta, gamma, omega2 or omega5-gliadins with two immunodominant epitopes on omega5-gliadin and a consensus motif of the type QQX1PX2QQ (X1 being L, F, S or I and X2 Q, E or G). One patient allergic to deamidated wheat proteins also had IgE to a repetitive peptide of gamma and omega2-gliadins of the type QPQQPFP. Sera from four patients with AEDS detected no linear epitopes on gliadins, despite the fact that they contained specific IgE to alpha, beta, gamma or omega-gliadins. One child with AEDS recognized cysteine-containing sequences in the nonrepetitive domains of alphabeta and gamma-gliadins. CONCLUSION B epitopes in wheat allergy were different from B epitopes of coeliac disease. Differences exist in IgE-binding epitopes between patients with food allergy to wheat. IgE from those suffering from WDEIA, anaphylaxis and urticaria detected sequential epitopes in the repetitive domain of gliadins whereas IgE from AEDS patients probably recognized conformational epitopes.

Published
2005

17. Inheritance of abnormal glycophorin C of the Gerbich and Yussef type in a French family

Author

J. Y. Muller, C. Schiffer, M.J. Loirat, D. Blanchard, and F. Pineau-Vincent

Subjects
Male, medicine.drug_class, Immunoblotting, Monoclonal antibody, Gerbich type, Antigen, hemic and lymphatic diseases, Agglutination Tests, medicine, Glycophorin, Humans, Glycophorins, Allele, Genetics, biology, Antibodies, Monoclonal, Hematology, General Medicine, Glycophorin C, Pedigree, Red blood cell, medicine.anatomical_structure, biology.protein, Blood Group Antigens, Female, France, Antibody
Abstract

The discovery of natural Gerbich antigen (anti-Ge2) in the serum of propositus prompted us to study his red blood cells (RBCs) by using monoclonal antibodies (mAbs) directed against glycophorin (GP) C and GPD. An mAb directed against the Ge4 antigen (mAb NaM10-7G11) agglutinated both untreated and trypsin-treated cells, demonstrating the expression of a trypsin-resistant GPC (namely, GPC of the Gerbich type : GPC Ge ). Surprisingly, an anti-Ge3 antibody (mAb NaM19-3C4) agglutinated untreated cells, showing that they also express the Ge3 antigen that may be carried by normal GPC and CPD or by the abnormal GPC of the Yussef (Yus) type (GPC Yus ). Immunoblotting analysis performed with an mAb directed against the C-terminal portion of GPC showed that the propositus' RBCs do not contain normal GPC and GPD but both GPC Ge and GPC Yus . Analysis of RBCs from the family demonstrated that, like the propositus, 2 of the 3 sisters had inherited both the GYPC Ge and the GYPC Yus alleles from the parents, who carried either the GYPC Ge or the GYPC Yus allele. The third sister had inherited the normal GYPC alleles from her parents, whereas the child of the propositus had inherited the GYPC Ge allele. Interestingly, natural anti-Ge2 antibodies were identified in the serum of 2 of the 3 Ge-negative individuals.

Published
1996

18. Epitope mapping on gliadins for patient sera reacting to modified gluten

Author

V. Leduc, F. Pineau, Martine Morisset, Chantal Brossard, Marie Bodinier, Thomas Mothes, F. Battais, Denise-Anne Moneret-Vautrin, Evelyne Paty, and Sandra Denery-Papini

Subjects
Pulmonary and Respiratory Medicine, chemistry.chemical_classification, Epitope mapping, Linear epitope, Biochemistry, chemistry, business.industry, Immunology, Immunology and Allergy, Medicine, business, Gluten
Published
2007

19. Un tableau clinique de lymphome de haut grade

Author

Ph. Renou, Cl. Schiffer, B Fabiani, F. Pineau-Vincent, A. Foulet, J. Dugay, and D Laneelle

Subjects
business.industry, Gastroenterology, Internal Medicine, Medicine, business
Published
1995

21. Trois observations d'auto anticorps dirigés contre l'activité procoagulante du facteur VIII (F VIIIc)

Author

F. Staikowsky, Ph. Moreau, F. Pineau-Vincent, Cl. Haas, and B. Guidet

Subjects
Penicillin, Chemistry, Gastroenterology, Internal Medicine, Autoantibody, medicine, Factor VIII Procoagulant Activity, Molecular biology, medicine.drug
Abstract

Auto antibodies against factor VIII procoagulant activity are uncommon. Three cases are reported in three different situations: peri-partum, penicillin and idiopatic inhibitor.

Published
1991

22. Comparaison de 4 auto-anticorps en pathologie thyroïdienne

Author

D. Tassy, M.F. Lonchampt, Ph. Renou, Cl. Schiffer, and F. Pineau-Vincent

Subjects
endocrine system, Pathology, medicine.medical_specialty, endocrine system diseases, biology, business.industry, Graves' disease, Thyroid, Gastroenterology, medicine.disease, Serum samples, Anti-thyroid autoantibodies, medicine.anatomical_structure, Immunology, Internal Medicine, medicine, biology.protein, Antibody, business, Lymphocytic Thyroiditis
Abstract

Four thyroid autoantibodies have been tested (antithyroglobulin, antimicrosomal, anti-thyroid peroxydase and anti-TSH-receptors antibodies) on four groups of serum samples (Graves disease, high suspicion of lymphocytic thyroiditis, non autoimmune thyroid diseases, controls). The diagnostic interest of each antibody is discussed.

Published
1991

23. Syndrome des antiphospholipides: 3 observations

Author

M. Lhuillier De Cordoze, I. Quere, Ph. Renou, Cl. Schiffer, J. Dugay, and F. Pineau-Vincent

Subjects
Systemic lupus erythematosus, biology, medicine.drug_class, business.industry, Anticoagulant, Gastroenterology, medicine.disease, Thrombosis, Primary antiphospholipid syndrome, Increased risk, immune system diseases, hemic and lymphatic diseases, Immunology, Internal Medicine, medicine, biology.protein, Anticardiolipin antibodies, cardiovascular diseases, Fetal loss, Antibody, skin and connective tissue diseases, business
Abstract

Antiphospholipid antibodies are associated with an increased risk of arterial or veinous thrombosis, fetal loss and thrombocytopenia. We report the case of 3 women: two of them have systemic lupus erythematosus, the third presents a primary antiphospholipid syndrome. Circulating lupus type anticoagulant, anticardiolipin antibodies are found in vitro.

Published
1991

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