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Introdução: A COVID-19 espalhou-se pelo mundo em 2020, tornando-se um problema de saúde pública mundial, responsável pela morte de milhares de pessoas em diversos países, especialmente os pais populosos, como o Brasil. Nessa perspectiva, o Sistema Único de Saúde no âmbito de suas diversas atribuições e complexidades, de modo a garantir o acesso integral e universal a saúde, em seus diversos níveis de atenção, reorganizou os cenários de modo a ofertar e atender às demandas necessárias diante do novo cenário. Objetivo: Avaliar a importância das estratégias de prevenção e condutas dos serviços de Atenção Primária a Saúde no enfrentamento da pandemia da COVID-19. Métodos: Trata-se de uma Revisão Sistemática da literatura e as plataformas literárias utilizadas para a busca dos periódicos foram LILACS e Pubmed. Discussão: A APS torna-se fundamental no controle da transmissão do COVID-19, pois detém de estratégias eficientes de contenção da transmissão, proteção e cuidado. Conclusão: A APS tem grande potencial de dar resolutividade aos casos suspeitos, com síndrome gripal e de manejar quadros leves, de modo a auxiliar na manutenção de leitos livres e diminuição dos custos ao sistema de saúde.

A deviated osteochondral fracture of the anterolateral tibia associated with fibular head avulsion in a 50-year-old patient is reported. In general, avulsion fracture of the iliotibial tract is associated with injuries in the cruciate ligament, in the meniscus and in lateral knee structures, as in the case herein reported.

Somatic resource banks play a crucial role in the conservation of genetic diversity, allowing for the preservation of biological samples from different populations. Puma somatic cells can be recovered from these banks and used in assisted techniques toward enhancing their multiplication and conservation. In response to the population reduction of this ecologically importance species, we aimed to evaluate the capacity of cryopreservation of somatic tissues on the maintenance of the integrity and quality of the cells recovered after culture, with the aim of establishing a somatic tissue bank that will allow for the safeguarding of a wide genetic sampling of pumas. Cryopreservation increased the thickness of the corneum layer in the tissues, and the number of perinuclear halos and empty gaps. Nevertheless, cryopreservation was able to maintain normal fibroblast patterns, even showing an increase in the percentage of collagen fibers. Cryopreservation maintained the proliferative potential of the tissues and the parameters evaluated during in vitro culture, mainly regarding the viability, proliferative activity, and apoptosis levels. Nevertheless, cells from cryopreserved tissues showed decreased metabolism and mitochondrial membrane potential when compared to cells from non-cryopreserved tissues. In summary, we demonstrated for the first time that puma somatic tissues subjected to cryopreservation are viable and maintain tissue integrity, featuring minimal changes after warming. Although viable somatic cells are obtained from these tissues, they undergo alterations in their metabolism and mitochondrial membrane potential. Improvements in the conservation conditions of somatic samples are needed to increase the quality of somatic tissue banks in this species.

The success of cloning by somatic cell nuclear transfer depends on the efficiency of nuclear reprogramming, with the cycle stage of the donor cell playing a crucial role. Therefore, the aim was to evaluate three different approaches for cell cycle synchronization: (i) serum starvation (SS) for 1 to 4 days, (ii) contact inhibition (CI) for 1 to 3 days, and (iii) using cell cycle regulatory inhibitors (dimethyl sulfoxide, cycloheximide, cytochalasin B, or 6-dimethylaminopurine) for 1 and 2 days, in terms of their effects on synchronization in G0/G1 phases and viability of collared peccary skin fibroblasts. Flow cytometry analysis revealed that SS for 4 days (79.0% ± 1.6) and CI for 3 days (78.0% ± 1.4) increased the percentage of fibroblasts in G0/G1 compared to growing cells GC (68.1% ± 8.6). However, SS for 3 and 4 days reduced the viability evaluated by differential staining (81.4% ± 0.03 and 81.6% ± 0.06) compared to growing cells (GC, 95.9% ± 0.06). CI did not affect the viability at any of the analyzed time intervals. No cell cycle inhibitors promoted synchronization in G0/G1. These results indicate that CI for 3 days was the most efficient method for cell cycle synchronization in peccary fibroblasts.

Clinicopathological features and chemotherapy can influence the quality of life (QOL), women with ovarian cancer. This study aimed to evaluate the physical and functional well-being, and ovarian cancer-specific effects scores reported from QOL questionnaire among women with ovarian cancer at the time of in their initial diagnosis and access the scores trajectory of women receiving neoadjuvant and adjuvant chemotherapy. This prospective study used cross-sectional analysis at baseline and longitudinal analysis from baseline to 12-month post-chemotherapy. QOL was evaluated at the baseline, at sixth cycle and 12-month post-chemotherapy using FACT-O questionnaire. Clinicopathological features and chemotherapy regime were evaluated and tested for associations with QOL measures. Of the 38 women enrolled in this study, 27 (80.1%) completed the questionnaire for 12 months. The multivariate linear regression results suggest, at the baseline, women with advance stage and presence of post-surgery residual disease showed lower scores in physical and functional well-being, ovarian cancer-specific effects, and FACT-O TOI domains (p

Objective: To describe the procedures before the intervention of occupational therapy with patients having communicative difficulties and hospitalized in an Intensive Care Unit (ICU). Method: Action research performed at an ICU of a reference hospital in the city of Rio de Janeiro, from June to December of 2018 in patients with restricted verbal communication, health professionals, and family members. We used the following instruments: 1) assessment form of the patient's clinical condition and biography; 2) registration of basic information at the bedside; 3) communication skills assessment protocol; 4) assessment of the patient's occupational profile supported by a set of Alternative and Extended Communication (AEC) boards; 5) patient satisfaction scale applied before and after the intervention. Results: Among the procedures, we highlight the actions before the entry of the professional in the unit and the outline of steps that were started with the active search; the preparation of the material and the necessary care; the use of the assessment protocol and communicative skills in the hospital context; occupational profile assessment; the offering and training of the basic AEC board kit; the assessment of patient satisfaction regarding their communicative ability; the registration in the medical record and guidance to family members and health professionals. Conclusion: The data showed that previous factors of the AEC should be considered and valued by the occupational therapist in favor of the management of communication of the patient with verbal restriction in the ICU.

This work describes the development of polysaccharide-coated liposomes to modulate the delivery of epidermal growth factor (EGF), with the aim to produce different EGF release profiles depending on the milieu of infected wounds. For this purpose, cationic liposomes were coated with one layer of sodium alginate (ALG) followed by one layer of chitosan (CHI) using the layer-by-layer (LbL) technique. The coated liposomes exhibited apparent hydrodynamic diameters of 278 ± 36 and 216 ± 96 nm for Lip-ALG and Lip-ALG-CHI, respectively. Thus, it appears that adding the CHI layer compacted the Lip-ALG one. The incorporation efficiency of EGF was a maximum of 55% for liposomes with a polymeric coating. In vitro release experiments showed that Lip-ALG-CHI exhibits a higher release rate constant under acidic pH conditions, resembling those of infected tissue. Using an ex vivo model of EGF release in porcine ear skin, these liposomes were found to accumulate in the epidermis. Thus, coated liposomes could represent a local EGF delivery mechanism to promote healing.

Objectives: The pregnancy period, with its peculiarities and specific symptoms that may or may not be physiological, can lead to medication use through prescription or even self-medication. This study aimed to assess self-medication practices among pregnant women, the most used medications, symptoms reported, and factors associated with this practice.Methods: This was a cross-sectional study conducted with pregnant women with an antenatal care (ANC) appointment in a tertiary teaching hospital referral in women’s health. From April 2019 to February 2020, 297 pregnant women were interviewed. Self-medication was considered as the use of any medicine (including medicinal plants (MPs), herbal products, and vitamins) without a medical or dental prescription. The period considered to assess self-medication practice was the last 60 days prior to the study interview.Results: Among the 297 women interviewed, 107 (36.0%) had practiced self-medication in the previous 60 days. Acetaminophen was the most used medication, and headache was the most frequent symptom reported by self-medicated pregnant women. Pregnant women with high-school (73 (68.2%) (OR = 2.52; 95% CI 1.17–5.43; p = 0.018)) or university-level (23 (21.5%) (OR = 2.82; 95% CI 1.15–6.94; p = 0.024)) education had a higher risk of practicing self-medication when compared to women with lower education. Women in the first gestational trimester (35 (32.7%) (OR = 3.61; 95% CI 1.64–7.96; p = 0.002)) and with two or more pregnancies (87 (81.2%) (OR = 1.96; 95% CI 1.07–3.60; p = 0.029)) were more likely to practice self-medication than pregnant women in the second or third gestational trimester and in the first pregnancy, respectively.Conclusion: Self-medication was practiced by a considerable proportion of our sample, with the majority being OTC drugs. The factors associated with self-medication can help to improve prevention strategies regarding self-medication during pregnancy.

BACKGROUND The literature shows no consensus on whether two- or three-segment surgically assisted rapid maxillary expansion is the best operative technique. METHODS The present clinical trial was designed to compare the outcome of two- and three-segment osteotomy surgically assisted rapid maxillary expansion. Thirty-two adult patients with transverse maxillary deficiency greater than or equal to 5 mm were randomly assigned to two- and three-segment groups (n = 16). Dimensional and psychological assessments (Oral Health Impact Profile and Brazilian Orthognathic Quality of Life Questionnaire) were carried out before surgical intervention and at one of the following time points: completion of expansion, removal of expanding device, 6 months after completion of expansion, or 10 months after completion of expansion. Dimensional assessments for asymmetric expansion of the maxilla and for changes in the area and volume of the palatine vault were performed on digital data from tomographic and laser scanning with the aid of an engineering inspection software. RESULTS No statistically significant differences were found in asymmetry or stability outcomes between groups. The psychological benefit provided by the three-segment technique did not spread through the domains of the quality-of-life questionnaires. CONCLUSIONS The current findings suggest that three-segment surgically assisted rapid maxillary expansion outcomes do not exceed those obtained with its two-segment counterpart regarding symmetry of maxillary expansion and stability of area and volume of the palatine vault. Furthermore, psychological nuances evidenced in two- and three-segment groups with the tools used play a limited, short-lasting role, or a specific, more sensitive assessment tool needs to be developed. CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic, II.

ResumoUma fratura osteocondral desviada da tíbia anterolateral associada a avulsão da cabeça da fíbula em um paciente de 50 anos é relatada. A fratura avulsão do trato iliotibial em geral está associada lesões do ligamento cruzado, do menisco, e das estruturas laterais do joelho, como no caso em questão.

Studies have suggested that meal timing plays a role in nutritional health, but this subject has not been sufficiently studied in pregnant women. We analysed the effect that timing of food intake has on eating patterns, diet quality and weight gain in a prospective cohort study with 100 pregnant women. Data were collected once per trimester: 4th–12th, 20th–26th and 30th–37th weeks. Food intake was evaluated using three 24-h dietary recalls, which were used to assess eating patterns and diet quality. Distribution of energy and macronutrient intake throughout the day was considered eating patterns. Diet quality was assessed using the Brazilian Healthy Eating Index-Revised. Weight gain was evaluated during each trimester. Women were classified as early or late timing of the first and last eating episodes if these values were below or above the median of the population, respectively (first eating episode = 08.38 hours; last eating episode = 20.20 hours). Generalised estimating equation models adjusted for confounders were used to determine the effects of timing of the first and last eating episodes (groups) and gestational trimesters (time) (independent variable) on eating patterns, diet quality and weight gain (dependent variables). Early eaters of the first eating episode have a higher percentage of energy and carbohydrate intake in morning and a lower at evening meals. They also have a better diet quality for fruit components when compared with late eaters of the first eating episode. Our results emphasise the importance of considering meal timing in the nutritional antenatal guidelines to promote maternal–fetal health.

A Leptospirose e uma doenca febril aguda causada pela espiroqueta Leptospira interrogans. Sua transmissao ocorre, principalmente, atraves do contato com a agua ou lama de enchentes contaminadas com urina de animais portadores, sobretudo os ratos. Nessa otica, foi realizado um estudo descritivo qualitativo com analise de dados secundarios disponiveis no Departamento de Informatica do SUS (DATASUS), sobre o perfil clinico-epidemiologico leptospirose no estado do Para entre os anos de 2012 a 2017. Os dados pesquisados foram analisados de acordo com as variaveis: faixa etaria, sexo, raca e evolucao clinica. Apos a analise dos dados, foram evidenciados um total de 782 notificacoes de casos da doenca, no periodo analisado. O grupo etario mais afetado foi o de 20 a 39 anos de idade. Em relacao ao sexo, observou-se predominio nos homens. Quanto a variavel cor, os pardos tiveram 73,9% indice de contagio. Do total de pessoas diagnosticadas com a doenca, 11,5% evoluiram para obito. Observou-se alta incidencia da doenca no periodo analisado, com predominância de adultos, homens e pardos. Esse perfil de acometimento pode ser explicado provavelmente pela maior exposicao desses grupos a doenca, bem como pela composicao da populacao do estado, com ampla preponderância de individuos pardos. Nesse sentido, faz-se necessaria a adocao, por parte das autoridades de Saude, de politicas voltadas a prevencao da doenca, por meio de acoes de educacao em saude sobre a enfermidade para a populacao, controle da populacao de roedores e melhorias no saneamento basico da regiao.

Objetivo: Relacionar prevalencia e adesao vacinal contra o HPV, em jovens, no municipio de Jatai-GO. Metodos: estudo transversal em base de dados secundarios nas plataformas Scielo, Pubmed e DATASUS, restritos a lingua portuguesa e ao recorte temporal 2010-2019. Utilizou-se os descritores “HPV”, “histopatologia” e “vacina”. Resultados: pode-se verificar que existe discrepância entre o numero de vacinados na primeira dose e na dose de reforco. Alem disso, ha desniveis quanto a vacinacao por genero, nota-se que o feminino e prevalente. Conclusao: Diante dos resultados, percebe-se que a adesao vacinal no municipio e baixa. Isso se deve a falta de conhecimento sobre os beneficios da vacinacao na prevencao de diversos cânceres relacionados ao HPV ou ainda esta associado aos pequenos efeitos colaterais da vacina em alguns individuos.

Introducao: Os pacientes internados em Unidade de Terapia Intensiva (UTI) sao comumente submetidos a sedacao, ventilacao mecânica, imobilidade e tempo de internacao prolongados, que podem acarretar em deficits neuromusculares e cognitivos. O Terapeuta Ocupacional (TO) busca minimizar essas condicoes que interferem diretamente desempenho ocupacional desses pacientes. Conhecer o cliente e suas capacidades funcionais a partir de instrumentos de funcionalidade podem auxiliar num melhor planejamento das intervencoes. Objetivo: Descrever o perfil funcional dos pacientes, bem como levantar as perdas funcionais mais recorrentes, e apontar as possiveis atuacoes da TO em pacientes criticos. Metodo: Estudo de coorte longitudinal que incluiu individuos com independencia total previa a internacao na UTI. Foram utilizados dois instrumentos que avaliam funcionalidade - a Perme Escore durante a internacao na UTI, e a MIF na alta da UTI. Resultados: Amostra de 18 pacientes, com idade media de 56 anos, 55,6% do genero feminino. A maior parte dos pacientes permaneceram somente no leito durante todo o periodo de internacao na UTI. Foram identificadas barreiras potenciais a mobilidade, relacionadas tanto ao proprio paciente, quanto em relacao a organizacao e cultura do servico. As intervencoes da TO englobaram aspectos motores, cognitivos, psicologicos, sensoriais e sociais. Apos alta da UTI, mais da metade dos pacientes foram capazes de realizar atividades fora do leito sem auxilio. Conclusao: O uso de avaliacoes coerentes com as caracteristicas do setor e da populacao atendida, contribuem para um tratamento mais estruturado, bem como possibilita identificar aspectos a serem melhorados, proporcionando um bom atendimento ao paciente critico. Abstract Introduction: Intensive Care Unit (ICU) patients are commonly submitted to prolonged sedation, mechanical ventilation, immobility and length of hospital stay, which can lead to neuromuscular and cognitive deficits. The Occupational Therapist (OT) seeks to minimize these conditions that directly interfere with the occupational performance of these patients. Knowing the client and his / her functional capacities from functional measurement tools can help in a better planning of the interventions. Objective: To describe the functional profile of the patients, as well as to obtain the most recurrent functional losses, and to point out the possible actions of OT in critical patients. Method: A longitudinal cohort study that included individuals with total independence prior to ICU admission. Two instruments were used to assess functionality - Perme Score during ICU stay, and FIM at ICU discharge. Results: A sample of 18 patients, with a mean age of 56 years, 55.6% of the female gender. Most of the patients remained only in bed during the entire ICU stay. Potential barriers to mobility were identified, related both to the patient and to the organization of the service. OT interventions included motor, cognitive, psychological, sensory and social aspects. After discharge from the ICU, more than half of the patients were able to perform activities outside the bed without assistance. Conclusion: The use of evaluations consistent with the characteristics of the sector and the population served contributes to a more structured treatment, as well as allows identifying aspects to be improved, providing a good care to critical patients. Keywords: Occupational Therapy, Intensive Care Units, Disabled Persons. Resumen Introduccion: Los pacientes internados en Unidad de Cuidados Intensivos (UCI) son comunmente sometidos a la sedacion, ventilacion mecanica, inmovilidad y tiempo de internacion prolongados, que pueden acarrear en deficit neuromusculares y cognitivos. El Terapeuta Ocupacional (TO) busca minimizar esas condiciones que interfieren directamente el desempeno ocupacional de estos pacientes. Conocer el cliente y sus capacidades funcionales a partir de instrumentos de evaluacion de funcionalidad pueden auxiliar en una mejor planificacion de las intervenciones. Objetivo: Describir el perfil funcional de los pacientes, asi como levantar las perdidas funcionales mas recurrentes, y senalar las posibles actuaciones de la TO en pacientes criticos. Metodo: Estudio de cohorte longitudinal que incluyo individuos con independencia total previa a la internacion en la UCI. Se utilizaron dos instrumentos que evaluan funcionalidad - la Perme Escore durante la internacion en la UCI, y la MIF en la alta de la UCI. Resultados: Muestra de 18 pacientes, con edad media de 56 anos, 55,6% del genero femenino. La mayoria de los pacientes permanecieron solo en el lecho durante todo el periodo de internacion en la UCI. Se identificaron barreras potenciales a la movilidad, relacionadas tanto al propio paciente, como a la organizacion del servicio. Las intervenciones de TO englobaron aspectos motores, cognitivos, psicologicos, sensoriales y sociales. Despues del alta de la UCI, mas de la mitad de los pacientes fueron capaces de realizar actividades fuera del lecho sin ayuda. Conclusion: El uso de evaluaciones coherentes con las caracteristicas del sector y de la poblacion atendida, contribuye a un tratamiento mas estructurado, asi como posibilita identificar aspectos a ser mejorados, proporcionando una buena atencion al paciente critico. Palabras clave: Terapia Ocupacional, Unidades de Cuidados Intensivos, Hospitalizacion.

A urolitíase é uma enfermidade de ampla incidência em cães. Este termo caracteriza a presença de urólitos, que consistem na união de cristais que se formam no trato urinário. A deposição destes cristais é decorrente de vários fatores, sejam estes adquiridos ou congênitos cursando em debilidade do animal quando ocorre obstrução podendo levar a morte quando um processo de intervenção não ocorre. A reincidência da patologia é alta para os animais onde se realizou intervenção cirúrgica sendo sua causa ainda não determinada. Sendo assim, o objetivo com a execução deste trabalho foi relatar um caso de reincidência de urolitíase em um cão da raça Pug atendido em clínica veterinária da região de Dourados – MS. O cão foi encaminhado para atendimento devido a alterações comportamentais observadas pelo proprietário e em anamnese realizada por veterinário foi constatado possível quadro de urolitíase já que este apresentava distensão e desconforto abdominal, hematúria e polaquiúria, procedendo-se então exames confirmatórios de raio-X e ultrassom para verificação da localização e número de cálculos. Realizou-se procedimento de cistotomia e acompanhamento clínico do animal, porém o mesmo apresentou três outras recidivas. Por meio do acompanhamento do animal e análise dos urólitos diagnosticou-se que a patologia estava associada à alimentação procedendo-se recomendações quanto a dieta e, ainda, em virtude de suas recidivas determinou-se que esta patologia também estava associada ao estresse sofrido pelo animal já que havia durante todas as ocorrências agentes estranhos no ambiente e após sua retirada houve melhora.

Made available in DSpace on 2020-12-12T02:24:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: The initiation and progression of periodontitis might involve a local renin-angiotensin system in periodontal tissue. This study hypothesized that Losartan treatment could promote protection to rats submitted to experimental periodontitis (EP) by attenuating alveolar bone loss due to reduction in inflammatory cytokines, better reactive oxidant species regulation and maintenance of the balance between bone formation and resorption factors. Methods: One hundred and thirty rats were submitted to EP with a silk suture thread (4.0) placed around the lower right first molar for 1, 3, 7, and 14 consecutive days. The study comprised four groups: G1—control without EP; G2—animals with EP treated with water; G3—Losartan-treated animals (treatment started at the same day of EP induction), and G4—animals previously treated with Losartan for 30 days followed by induction of EP and continuity of treatment. Results: G2 rats had greater bone loss volume, increased number, and thickness and decreased separation of trabeculae. On the other hand, G4 animals showed significant improvements in these parameters. Histological analysis revealed that EP favors inflammatory cell infiltration and junctional epithelium, cementum with alveolar bone crest destruction, but animals pretreated with Losartan (G4) did not show these features. Although the G3 animals did not demonstrate the improvements detected in G4, mRNA expression results were similar. In mandibular tissue, EP promoted mRNA increases for ACE, AT1 receptor, and inflammatory mediators as well as decreases for antioxidant enzymes. However, Losartan treatments attenuated these responses in addition to promoting an increase in bone formation markers and transcription factors. Conclusion: AT1 receptor modulates EP progression. Department of Biological Sciences Bauru School of Dentistry University of São Paulo Department of Basic Sciences School of Dentistry São Paulo State University-UNESP Department of Biomedical Engineering Medical College of Wisconsin Department of Basic Sciences School of Dentistry São Paulo State University-UNESP FAPESP: 2015/03965-2

Define-se neonatologia como a ciência responsável pelo estudo dos cuidados aos neonatos, aos seus aspectos fisiológicos e às suas afecções neonatais propriamente ditas. O sistema de escore Apgar é um método fácil e confiável para avaliar a vitalidade de neonatos animais. No presente trabalho objetivou-se avaliar a vitalidade de neonatos caninos nascidos de parto eutócico e cesariana. Foram coletados dados de 31 neonatos de cães de diferentes portes e raças. Desse número amostral, 21 nasceram de parto eutócico e 10 através de cesariana. Coletaram-se os dados cerca de 10 minutos após o nascimento, aferindo-se: frequências cardíacas, respiratórias e inspecionados a coloração da mucosa gengival, tônus muscular e irritabilidade reflexa, atribuindo-lhes notas de 0 a 2. A somatória das notas específica de cada variável conferiu aos neonatos o escore Apgar total de 0 a 10. Os resultados foram submetidos à análise de variância ao nível de 5% de probabilidade e realizou-se o teste de normalidade e homogeneidade. As variáveis foram comparadas pelo teste t- Student, ao nível de 5% de probabilidade. As frequências cardíaca, respiratória e escore Apgar foram influenciados pelos tipos de parto. As maiores médias de frequências cardíacas, respiratórias e escore Apgar de 184,05 ± 6,95 bpm e 47,23 ± 2,18 mpm e 8,95 ± 0,22, respectivamente, foram encontrados em animais nascidos de parto eutócico. O escore Apgar demonstrou-se um método eficiente na avaliação neonatal de cães, bem como colaborou para identificação de alterações inespecíficas do período neonatal imediato.

A mismatch between circadian and social clocks leads to a circadian misalignment, which has been widely measured by social jetlag (SJL). There are several studies measuring SJL, but it has not been studied in pregnant women. Therefore, this study aimed to identify the occurrence of SJL throughout pregnancy and to verify whether there is an effect of pre-pregnancy body mass index (BMI) on SJL throughout pregnancy. The baseline of the present study was conducted with 205 1st trimester pregnant women of whom 100 were followed in their 2nd and 3rd trimester. SJL was calculated based on the absolute difference between mid-sleep time on workdays versus work-free days. The pre-pregnancy BMI and current BMI (kg/m2) were calculated. Linear regression and Generalised Estimating Equation (GEE) adjusted for confounders were used to determine the association between SJL and the gestational trimesters (time), and anthropometric variables. Most of the pregnant women (54.5%) presented SJL > 1 h in the first gestational trimester. We also found an isolated effect of the gestation trimester on the SJL mean. In this sense, pregnant women had a decrease in SJL from the second to the third trimester (1.33 ± 0.08 versus 1.12 ± 0.07, respectively; p = 0.012). GEE analyzes showed that pregnant women of a normal weight showed a decrease in SJL from the second to the third trimester (1.29 ± 0.11 and 0.93 ± 0.08, respectively, p = 0.032), but this was not found in the other groups of nutritional status (underweight, overweight and obesity). In addition, a positive association between SJL and pre-gestational BMI in the third trimester (β = 0.200, p = 0.046) was found. SJL is quite prevalent during the gestational period and excessive BMI both before and during pregnancy is associated with an increased risk of having SJL > 1 h in the third and second trimesters, respectively. In addition, pregnant women of normal weight—but not underweight or overweight—had decreased SJL from the second to the third trimester.

Carbohydrate (CHO) mouth rinsing seems to improve performance in exercises lasting 30–60 min. However, its effects on intermittent exercise are unclear. It is also unknown whether serial CHO mouth rinses can promote additional ergogenic effects when compared with a single mouth rinse. The aim of this study was to evaluate the effect of single and serial CHO mouth rinses on Yo-Yo Intermittent Recovery Test Level 1 (Yo-Yo IR1) performance in soccer players. In a randomized, crossover, double-blind, placebo-controlled design, 12 male (18.9 ± 0.5 years) soccer players performed eight serial mouth rinses under three different conditions: placebo solution only (noncaloric juice), seven placebo mouth rinses plus a single CHO mouth rinse (8% maltodextrin), or eight CHO mouth rinses (8-CHO). Following the final mouth rinse, individuals performed the Yo-Yo IR1 test to evaluate the maximal aerobic endurance performance measured via total distance covered. There were no differences in Yo-Yo IR1 performance between sessions (p = .32; single CHO mouth rinse (8% maltodextrin): 1,198 ± 289 m, eight CHO mouth rinses: 1,256 ± 253 m, placebo: 1,086 ± 284 m). In conclusion, single and serial CHO mouth rinsing did not improve performance during the Yo-Yo IR1 for soccer players. These data suggest that CHO mouth rinsing is not an effective ergogenic strategy for intermittent exercise performance irrespective of the number of rinses.

The aim of the present study was to verify if a protocol for cleaning the oral cavity of infants in the pre-dental period can reduce extrinsic salivary metabolites observed through Nuclear Magnetic Resonance (NMR). A cross-sectional clinical study with a convenience sample was conducted, and infants were recruited at the UFRJ Pediatric Dentistry Clinic. Participants who had used antibiotics and/or antifungals up to 3 months before and whose legal guardians did not consent or sign the Informed Consent Form were excluded. An anamnesis was performed with the guardians and the participants' intraoral clinical examination. Initial collection of unstimulated total saliva was performed using an automatic pipette with sterile plastic tips in the buccal floor region, at least 1 h after the last feeding. Subsequently, the infants' oral mucosa was cleaned with gauze moistened with filtered water, and after 5 min, a new collection was performed, using the same methodology. The obtained samples were immediately transferred on ice to the laboratory, centrifuged (10,000 g), and stored at −80°C. The NMR analyses were performed using a 500-MHz spectrometer Bruker, Germany); evaluations were done via the 1H and 1H-1H TOCSY spectra for metabolite signaling. Eleven pre-dental infants were evaluated, with a mean age of 3.8 months, including six girls (55%). Of these, nine participants (82%) were exclusively breastfed. The higher presence of components such as lactose, glucose, sugars, acetate, alanine, and lactate were observed in the samples before oral mucosa cleaning. Regarding the type of diet, more lactose was observed in the saliva of patients who were exclusively breastfed than those that received mixed feeding. We conclude that the oral mucosa cleaning of infants in the pre-dental period tends to reduce the concentration of extrinsic components from the diet, such as lactose, in the salivary metabolomic profile analyzed by NMR.

A 4-year-old male mixed-breed cat was evaluated with a history of the presence of a reddish mass in the left eye medial corner. Ophthalmic examination revealed prolapse of the third eyelid gland, conjunctival secretion and hyperemia. The animal underwent gland replacement surgery using the modified Morgan technique. After surgery, clinical signs improved on the first day. The cat was observed for 2 years after surgery and there was no recurrence of the condition. The surgical technique was effective in resolving the condition., Um felino de quatro anos de idade, macho e sem raça definida foi atendido com histórico de surgimento de uma massa avermelhada no canto medial do bulbo ocular esquerdo. Ao exame oftalmológico foi observado o prolapso da glândula da terceira pálpebra, hiperemia e secreção em conjuntiva. O animal foi submetido ao procedimento cirúrgico de reposição da glândula pela técnica de Morgan modificada. Após a cirurgia, notou-se melhora dos sinais clínicos. O animal foi acompanhado durante 3 anos após o procedimento e não houve, até o presente momento, a recidiva do prolapso da glândula. Esse é o primeiro caso relatado no Brasil de prolapso da glândula terceira pálpebra em felino. A técnica cirúrgica utilizada foi efetiva na resolução da condição.

Demodicose é uma dermatose parasitária considerada incomum no gato doméstico. Este estudo tem como objetivo relatar o primeiro sucesso terapêutico do sarolaner por via oral no tratamento de Demodex spp. em um gato naturalmente infestado no Brasil. Um gato adulto, positivo para o vírus da leucemia felina e em tratamento com glicocorticóides, apresentava sinais clínicos da pele como prurido intenso, hiperceratose, lignificação, fragilidade da pele e alopecia simétrica. O diagnóstico foi confirmado em arranhões na pele, impressão em tiras de acetato e exame coproparasitológico. Após o diagnóstico, o sarolaner foi administrado por via oral (4mg / kg). A avaliação foi realizada com arranhões na pele 24, 48 horas e 7, 14, 21 e 28 dias após o tratamento. Após 21 dias, nenhum ácaro foi observado e houve uma melhora na fragilidade da pele e na repilação parcial. Com base nos resultados deste estudo, pode-se concluir que o sarolaner foi eficaz no tratamento da sarna demodécica felina., Demodicose é uma dermatose parasitária considerada incomum no gato doméstico. Este estudo tem como objetivo relatar o primeiro sucesso terapêutico do sarolaner, por via oral, no tratamento de Demodex spp. em um gato naturalmente infestado no Brasil. Um gato adulto, positivo para vírus da leucemia felina e no tratamento com glicocorticóides, apresenta sinais clínicos de pele como prurido intenso, hiperqueratose, lignificação, fragilidade da pele e alopecia simétrica. O diagnóstico foi confirmado em raspados cutâneos, imprimindo em fita de acetato e sem exame coproparasitológico. Após o diagnóstico, o sarolaner foi administrado por via oral (4mg / kg). Uma avaliação foi realizada com raspados cutâneos com 24 e 48 horas e com 7, 14, 21 e 28 dias após o tratamento. Após 21 dias, nenhum óleo foi observado e houve uma melhora na fragilidade da pele e com a reabilitação parcial. Com base nos resultados deste estudo, pode-se concluir que o sarolaner foi eficaz no tratamento da sarna demodécica felina.

To determine the association between cephalometric measurements and polysomnographic parameters in Brazilian patients with midface deficiency. This was a primary, clinical, observational, longitudinal, retrospective, analytical, and single-center study. Forty-eight patients with midface deficiency were divided into two groups as follows: those who underwent surgically assisted rapid palatal expansion (SARME) and those who received maxillary advancement (MA). Pre- and post-operative cephalometric and polysomnography measurements were obtained. Pearson's correlation was used to verify the presence of any significant associations between PSG scores and cephalometric measurements. Associations between BMI (Body Mass Index) and AHI (Apnea Hypopnea Index) as well as arousals were observed. In the SARME group, associations between AHI and SNA, UAS and MP-H, arousals and SNA, and Co-A and MP-H were noted. Associations between AHI and Co-A, PoOr-A and MP-H, arousals and UAS, and between minimum saturation of O2 and SNA, SNB, and Co-A were observed in the MA group. This study demonstrates the alterations in the middle third of the face that were related to sleep disturbance. In addition, it shows the associations between the polysomnographic parameters and the cephalometric representations corresponding to the analyzed deformities and transverse or anteroposterior maxillary deficiencies.

Low-grade inflammation associated with metabolic syndrome (MS) triggers alterations in several organs, but its effects on pulmonary functional and immunological response in older adults are unknown. This cross-sectional study investigated such responses in older adults with and without MS. The study consisted of 77 older adults with MS (68 ± 3 years old) and without MS (67 ± 3 years old). Impulse oscillometry (IOS) was used to evaluate airway and tissue resistance and reactance. Biomarkers of systemic and pulmonary inflammation and fibrosis were studied. Total resistance of respiratory system (R5Hz; p

Otodectes cynotis is a mite with a cosmopolitan distribution that is the primary agent for the development of otitis externa in feline species. The aim of this study was to evaluate the efficacy of the oral administration of sarolaner for the treatment of feline otodectic mange. We used 20 adult cats of both sexes that were naturally infested with O. cynotis. The mite infestation scoring was performed by video-otoscopy before treatment. The cats were randomized according to the infestation score and divided into two groups (treated and control). The treated group underwent oral administration of sarolaner in a single dose of 2–4 mg/kg. The evaluations were performed by video-otoscopy to evaluate the reduction of infestation score 2, 4, 6, 24 and 48 h and 7, 14, 21 and 28 days after medication. At the end of the study, the cats were sedated to enable the recovery of live and dead mites to determine efficacy. No adverse effects or laboratory changes were observed in these cats. Sarolaner showed 100% efficacy 48 h after treatment. Based on the results, a single oral dose of sarolaner was effective in controlling otodectic mange in naturally infested cats.

Aims To evaluate the applicability of the Mimosa tenuiflora and Eucalyptus urograndis pyroligneous acids (PAs) as alternative antiseptics in dairy goats. Methods and results Cytotoxicity was evaluated in vitro using bacteria, as well as in vivo using goats, and the influence of PAs on the physicochemical parameters of fresh milk were examined. The cytotoxicity of PAs was evaluated in terms of morphology, cell viability, and metabolic activity of goat tegumentary cells. The PA of M. tenuiflora had results similar to those of 2% iodine. For the in vitro tests, strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were used with the well technique, demonstrating inhibition halos greater than 9 mm. In the in vivo test, 15 animals were used per phase of the experiment, and the plate counting technique showed that there was antiseptic action of both extracts, with emphasis on the M. tenuiflora PA. Physicochemical analysis of the milk showed that neither PAs interfered with its physical-chemical parameters. Conclusions The PA of M. tenuiflora presented potential as an alternative antiseptic in dairy goats. Significance and impact of the study This study demonstrates the use of PA as an antimicrobial agent in animals.

Resumo A pandemia de Covid-19 tem provocado a adoção de medidas que ocasionam mudança na rotina e na organização do trabalho das universidades. Os ambientes virtuais vêm se apresentando como vetores para os cursos de graduação durante o enfrentamento da pandemia, sendo necessário produzir reflexões para este novo contexto. A Rede Nacional de Ensino e Pesquisa em Terapia Ocupacional – RENETO, Brasil, atenta às questões implicadas com a formação e produção de conhecimento da área, vem buscando, junto aos pares, maneiras de realizar uma leitura complexa da realidade e, com base nesta, criar e implementar estratégias que deem conta de atender as demandas impostas, respeitando as regionalidades e naturezas institucionais em que os cursos de graduação estão inseridos. Assim, com base em um levantamento realizado junto às coordenações de cursos de graduação em terapia ocupacional, a fim de compreender como os cursos de graduação têm aderido ou não à oferta de ensino por meio de plataformas digitais, o presente trabalho reflete acerca das tomadas de decisões pedagógicas, éticas e políticas no âmbito das diversas atividades acadêmicas, não apenas no contexto do enfrentamento da pandemia, como também nas projeções da nova realidade imposta por esta problemática. Abstract The Covid-19 pandemic has led to the adoption of measures that bring about changes in the routine and organization of work in universities. The virtual environments have been considered vectors for undergraduate courses during the pandemic, and it is necessary to reflect on this new context. The Rede Nacional de Ensino e Pesquisa em Terapia Ocupacional – RENETO (National Network of Education and Research in Occupational Therapy), Brazil, attentive to the issues involved in the formation and production of knowledge in the field, has been searching with peers for ways to carry out a complex reading of reality and, from this, create and implement strategies to be able to deal with the demands imposed, respecting the regionalities and institutional nature in which the undergraduate courses are running on. Thus, based on a survey carried out with the coordinators of Occupational Therapy courses, which aimed to understand how the undergraduate courses have adhered or not to teaching through digital platforms, this paper reflects on decision making about pedagogical, ethical, and political dimensions in the scope of academic activities not only in the context of the pandemic, but also considering the projections of the new reality imposed by the pandemic.

OBJECTIVE This randomized clinical trial aimed to compare the outcomes of the mandibular overdenture retained by one (1-IOD) or two (2-IOD) implants. MATERIALS AND METHODS Participants received new complete dentures, were assessed at baseline and randomly assigned to groups. Early loaded single midline implant (1-IOD) or two implants in the canine regions bilaterally (2-IOD). Ball attachments were used for overdenture retention. Post-treatment outcomes (6- and 12-month follow-ups) included patient satisfaction, oral health-related quality of life measures, and chewing function. Data analysis included within- and between-group comparisons, and Generalized Estimating Equations. Both superiority and non-inferiority hypotheses were tested. RESULTS Forty-seven participants were included (1-IOD = 23; 2-IOD = 24). Significant improvements in OHIP-Edent were observed after insertion of new dentures and at the 1-year follow-up compared with baseline. No differences were found between the 1- and 2-IOD groups for the OHIP-Edent and QoLFAST scores. Patient satisfaction with the mandibular denture improved significantly from baseline to the 6-month follow-up and remained unaltered until 1 year, with no differences between groups. The magnitudes of treatment effect sizes were moderate to large for patient-reported outcomes. Progressive improvement in chewing function occurred in both groups. Non-inferiority testing based on a 15% non-inferiority margin showed inconclusive results for patient-reported outcomes, whilst chewing function in the 1-IOD group was concluded to be not inferior to the 2-IOD group. CONCLUSIONS Results support the use of both 1- and 2-IOD. The 1-IOD was also an acceptable alternative to the 2-IOD as a secondary option for the treatment of the edentulous mandible.

A pandemia de COVID-19 tem provocado a adoção de medidas que ocasionam a mudança na rotina e na organização do trabalho das universidades. Os ambientes virtuais vêm se apresentando como vetores para os cursos de graduação durante o enfrentamento da pandemia, sendo necessário produzir reflexões para este novo contexto. A Rede Nacional de Ensino e Pesquisa em Terapia Ocupacional – RENETO, Brasil, atenta as questões implicadas com a formação e produção de conhecimento da área, vem buscando junto aos pares maneiras de realizar uma leitura complexa da realidade e, a partir desta, criar e implementar estratégias que deem conta de atender as demandas impostas, respeitando as regionalidades em que os cursos de graduação estão inseridos. Assim, a partir de um levantamento realizado junto às coordenações de cursos de graduação em Terapia Ocupacional, a fim de compreender como os cursos de graduação têm aderido ou não à oferta de ensino por meio de plataformas digitais, o presente trabalho pretende refletir acerca das tomadas de decisões pedagógicas, éticas e políticas no âmbito das diversas atividades acadêmicas, não apenas no contexto do enfrentamento da pandemia, como também nas projeções da nova realidade imposta por esta problemática. Contudo, observa-se ainda um cenário um tanto desarticulado na área, entre instituições significativamente diferentes, públicas e privadas.

Background Biobanking of cell lines is a promising tool of support for wildlife conservation. In particular, the ability to preserve fibroblast cell lines derived from collared peccaries is of significance as these wild mammals are unique to the Americas and play a large role in maintaining the ecosystem. We identified collared peccary fibroblasts by immunofluorescence and evaluated their morphology, growth and adherence capacity. Further, we monitored the viability and metabolic activity of the fibroblasts to determine the effects of passage number and cryopreservation on establishment of cell lines. Methods Skin biopsies were collected from the peripheral ear region from five adult animals in captivity. Initially, cells were isolated from fragments and cultured in the Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum and 2% antibiotic–antimycotic solution under a controlled atmosphere (38.5 °C, 5% CO2). We evaluated the maintenance of primary cells for morphology, adherence capacity of explants, explants in subconfluence, cell growth and absence of contamination. Moreover, we identified the fibroblast cells by immunofluorescence. Additionally, to evaluate the influence of the number of passages (first, third and tenth passage) and cryopreservation on establishment of cell lines, fibroblasts were analysed for the viability, metabolic activity, population doubling time (PDT), levels of reactive oxygen species (ROS), and mitochondrial membrane potential (ΔΨm). Results All explants (20/20) adhered to the dish in 2.4 days ± 0.5 with growth around the explants in 4.6 days ± 0.7, and subconfluence was observed within 7.8 days ± 1.0. Moreover, by morphology and immunocytochemistry analyses, cells were identified as fibroblasts which presented oval nuclei, a fusiform shape and positive vimentin staining. No contamination was observed after culture without antibiotics and antifungals for 30 days. While there was no difference observed for cell viability after the passages (first vs. third: P = 0.98; first vs. tenth: P = 0.76; third vs. tenth: P = 0.85), metabolic activity was found to be reduced in the tenth passage (23.2 ± 12.1%) when compared to that in the first and third passage (100.0 ± 24.4%, P = 0.006). Moreover, the cryopreservation did not influence the viability (P = 0.11), metabolic activity (P = 0.77), or PDT (P = 0.11). Nevertheless, a greater ΔΨm (P = 0.0001) was observed for the cryopreserved cells (2.12 ± 0.14) when compared to that in the non-cryopreserved cells (1.00 ± 0.05). Additionally, the cryopreserved cells showed greater levels of intracellular ROS after thawing (1.69 ± 0.38 vs. 1.00 ± 0.22, P = 0.04). Conclusions This study is the first report on isolation, characterization and cryopreservation of fibroblasts from collared peccaries. We showed that adherent cultures were efficient for obtaining fibroblasts, which can be used as donor cells for nuclei for species cloning and other applications.