1. Fragment-based discovery of a chemical probe for the PWWP1 domain of NSD3
- Author
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Xiao-Ling Cockcroft, Stephan Karl Zahn, Mark Petronczki, Catherine M. Rogers, Fengling Li, Helmut Berger, Bernadette Sharps, Markus Zeeb, Ralph A. Neumüller, Mark Pearson, Dalia Barsyte-Lovejoy, Teresa Krammer, Oleg Fedorov, Barbara Müllauer, Alexander Weiss-Puxbaum, Kilian Huber, Masoud Vedadi, Magdalena M. Szewczyk, Christopher R. Vakoc, Abdellah Allali-Hassani, Tobias Wunberg, Steven Kennedy, Christoph Reiser, Michael Schleicher, Julian E. Fuchs, Cheryl H. Arrowsmith, Moriz Mayer, Jark Böttcher, Guido Boehmelt, Dietrich Böse, Alexandra Hörmann, Andreas Zoephel, Heribert Arnhof, Sandra Winkler, Darryl B. McConnell, Peter Brown, David Dilworth, Maja Corcokovic, Klaus Rumpel, Thomas Gerstberger, Nikolai Mischerikow, Carrow I. Wells, Ulrich Reiser, and Daniela Häring
- Subjects
Messenger RNA ,Cell Survival ,Chemistry ,Protein domain ,Nuclear Proteins ,Myeloid leukemia ,Histone-Lysine N-Methyltransferase ,Cell Biology ,Amplicon ,medicine.disease ,In vitro ,Cell Line ,Chromatin ,Cell biology ,Proto-Oncogene Proteins c-myc ,Leukemia ,Gene Expression Regulation ,Protein Domains ,medicine ,Humans ,CRISPR-Cas Systems ,Binding site ,Molecular Biology ,Cell Proliferation - Abstract
Here, we report the fragment-based discovery of BI-9321, a potent, selective and cellular active antagonist of the NSD3-PWWP1 domain. The human NSD3 protein is encoded by the WHSC1L1 gene located in the 8p11-p12 amplicon, frequently amplified in breast and squamous lung cancer. Recently, it was demonstrated that the PWWP1 domain of NSD3 is required for the viability of acute myeloid leukemia cells. To further elucidate the relevance of NSD3 in cancer biology, we developed a chemical probe, BI-9321, targeting the methyl-lysine binding site of the PWWP1 domain with sub-micromolar in vitro activity and cellular target engagement at 1 µM. As a single agent, BI-9321 downregulates Myc messenger RNA expression and reduces proliferation in MOLM-13 cells. This first-in-class chemical probe BI-9321, together with the negative control BI-9466, will greatly facilitate the elucidation of the underexplored biological function of PWWP domains. A chemical probe BI-9321 for the PWWP1 domain of NSD3 and its inactive analog were identified. BI-9321 binds to the methyl-lysine binding site, reduces the association of NSD3 with chromatin and inhibits proliferation of acute myeloid leukemia cells.
- Published
- 2019