43 results on '"Takuya Ishida"'
Search Results
2. Diagnostic Utility of Specific Frailty Questionnaire: The Kihon Checklist for Hippocampal Atrophy in COPD
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Tsunahiko Hirano, Shun Takahashi, Ayumi Fukatsu-Chikumoto, Kasumi Yasuda, Takuya Ishida, Tomohiro Donishi, Kazuyoshi Suga, Keiko Doi, Keiji Oishi, Shuichiro Ohata, Yoriyuki Murata, Yoshikazu Yamaji, Maki Asami-Noyama, Nobutaka Edakuni, Tomoyuki Kakugawa, and Kazuto Matsunaga
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Kihon Checklist ,hippocampal atrophy ,diagnostic utility ,COPD ,daily inactivity ,Medicine - Abstract
Background/Objectives: COPD patients who are frail have been reported to develop brain atrophy, but no non-invasive diagnostic tool has been developed to detect this condition. Our study aimed to explore the diagnostic utility of the Kihon Checklist (KCL), a frailty questionnaire, in assessing hippocampal volume loss in patients with COPD. Methods: We recruited 40 COPD patients and 20 healthy individuals using the KCL to assess frailty across seven structural domains. Hippocampal volumes were obtained from T1-weighted MRI images, and ROC analysis was performed to detect hippocampal atrophy. Results: Our results showed that patients with COPD had significantly greater atrophic left hippocampal volumes than healthy subjects (p < 0.05). The univariate correlation coefficient between the left hippocampal volume and KCL (1–20), which pertains to instrumental and social activities of daily living, was the largest (ρ = −0.54, p < 0.0005) among the KCL subdomains. Additionally, both KCL (1–25) and KCL (1–20) demonstrated useful diagnostic potential (93% specificity and 90% sensitivity, respectively) for identifying individuals in the lowest 25% of the left hippocampal volume (AUC = 0.82). Conclusions: Our study suggests that frailty questionnaires focusing on daily vulnerability, such as the KCL, can effectively detect hippocampal atrophy in COPD patients.
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- 2024
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3. Real-time dynamic adsorption processes of cytochrome c on an electrode observed through electrochemical high-speed atomic force microscopy.
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Kouta Takeda, Takayuki Uchihashi, Hiroki Watanabe, Takuya Ishida, Kiyohiko Igarashi, Nobuhumi Nakamura, and Hiroyuki Ohno
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Medicine ,Science - Abstract
An understanding of dynamic processes of proteins on the electrode surface could enhance the efficiency of bioelectronics development and therefore it is crucial to gain information regarding both physical adsorption of proteins onto the electrode and its electrochemical property in real-time. We combined high-speed atomic force microscopy (HS-AFM) with electrochemical device for simultaneous observation of the surface topography and electron transfer of redox proteins on an electrode. Direct electron transfer of cytochrome c (cyt c) adsorbed on a self-assembled monolayers (SAMs) formed electrode is very attractive subject in bioelectrochemistry. This paper reports a real-time visualization of cyt c adsorption processes on an 11-mercaptoundecanoic acid-modified Au electrode together with simultaneous electrochemical measurements. Adsorbing cyt c molecules were observed on a subsecond time resolution simultaneously with increasing redox currents from cyt c using EC-HS-AFM. The root mean square roughness (RRMS) from the AFM images and the number of the electrochemically active cyt c molecules adsorbed onto the electrode (Γ) simultaneously increased in positive cooperativity. Cyt c molecules were fully adsorbed on the electrode in the AFM images when the peak currents were steady. This use of electrochemical HS-AFM significantly facilitates understanding of dynamic behavior of biomolecules on the electrode interface and contributes to the further development of bioelectronics.
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- 2015
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4. Characterization of a novel PQQ-dependent quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea classified into auxiliary activities family 12 in carbohydrate-active enzymes.
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Kouta Takeda, Hirotoshi Matsumura, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida, Kiyohiko Igarashi, and Nobuhumi Nakamura
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Medicine ,Science - Abstract
The basidiomycete Coprinopsis cinerea contains a quinohemoprotein (CcPDH named as CcSDH in our previous paper), which is a new type of pyrroloquinoline-quinone (PQQ)-dependent pyranose dehydrogenase and is the first found among all eukaryotes. This enzyme has a three-domain structure consisting of an N-terminal heme b containing a cytochrome domain that is homologous to the cytochrome domain of cellobiose dehydrogenase (CDH; EC 1.1.99.18) from the wood-rotting basidiomycete Phanerochaete chrysosporium, a C-terminal family 1-type carbohydrate-binding module, and a novel central catalytic domain containing PQQ as a cofactor. Here, we describe the biochemical and electrochemical characterization of recombinant CcPDH. UV-vis and resonance Raman spectroscopic studies clearly reveal characteristics of a 6-coordinated low-spin heme b in both the ferric and ferrous states, as well as intramolecular electron transfer from the PQQ to heme b. Moreover, the formal potential of the heme was evaluated to be 130 mV vs. NHE by cyclic voltammetry. These results indicate that the cytochrome domain of CcPDH possesses similar biophysical properties to that in CDH. A comparison of the conformations of monosaccharides as substrates and the associated catalytic efficiency (kcat/Km) of CcPDH indicates that the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation. Furthermore, a binding study shows a high binding affinity of CcPDH for cellulose, suggesting that CcPDH function is related to the enzymatic degradation of plant cell wall.
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- 2015
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5. Multidimensional QoE of Multiview Video and Selectable Audio IP Transmission
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Toshiro Nunome and Takuya Ishida
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Technology ,Medicine ,Science - Abstract
We evaluate QoE of multiview video and selectable audio (MVV-SA), in which users can switch not only video but also audio according to a viewpoint change request, transmitted over IP networks by a subjective experiment. The evaluation is performed by the semantic differential (SD) method with 13 adjective pairs. In the subjective experiment, we ask assessors to evaluate 40 stimuli which consist of two kinds of UDP load traffic, two kinds of fixed additional delay, five kinds of playout buffering time, and selectable or unselectable audio (i.e., MVV-SA or the previous MVV-A). As a result, MVV-SA gives higher presence to the user than MVV-A and then enhances QoE. In addition, we employ factor analysis for subjective assessment results to clarify the component factors of QoE. We then find that three major factors affect QoE in MVV-SA.
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- 2015
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6. Alterations of Regional Cerebral Blood Flow in Tinnitus Patients as Assessed Using Single-Photon Emission Computed Tomography.
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Takashi Ueyama, Tomohiro Donishi, Satoshi Ukai, Yuta Yamamoto, Takuya Ishida, Shunji Tamagawa, Muneki Hotomi, Kazuhiro Shinosaki, Noboru Yamanaka, and Yoshiki Kaneoke
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Medicine ,Science - Abstract
Tinnitus is the perception of phantom sound without an external auditory stimulus. Using neuroimaging techniques, such as positron emission tomography, electroencephalography, magnetoencephalography, and functional magnetic resonance imaging (fMRI), many studies have demonstrated that abnormal functions of the central nervous system are closely associated with tinnitus. In our previous research, we reported using resting-state fMRI that several brain regions, including the rectus gyrus, cingulate gyrus, thalamus, hippocampus, caudate, inferior temporal gyrus, cerebellar hemisphere, and medial superior frontal gyrus, were associated with tinnitus distress and loudness. To reconfirm these results and probe target regions for repetitive transcranial magnetic stimulation (rTMS), we investigated the regional cerebral blood flow (rCBF) between younger tinnitus patients (
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- 2015
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7. Discovery of a eukaryotic pyrroloquinoline quinone-dependent oxidoreductase belonging to a new auxiliary activity family in the database of carbohydrate-active enzymes.
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Hirotoshi Matsumura, Kiwamu Umezawa, Kouta Takeda, Naohisa Sugimoto, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida, Kiyohiko Igarashi, and Nobuhumi Nakamura
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Medicine ,Science - Abstract
Pyrroloquinoline quinone (PQQ) is a redox cofactor utilized by a number of prokaryotic dehydrogenases. Not all prokaryotic organisms are capable of synthesizing PQQ, even though it plays important roles in the growth and development of many organisms, including humans. The existence of PQQ-dependent enzymes in eukaryotes has been suggested based on homology studies or the presence of PQQ-binding motifs, but there has been no evidence that such enzymes utilize PQQ as a redox cofactor. However, during our studies of hemoproteins, we fortuitously discovered a novel PQQ-dependent sugar oxidoreductase in a mushroom, the basidiomycete Coprinopsis cinerea. The enzyme protein has a signal peptide for extracellular secretion and a domain for adsorption on cellulose, in addition to the PQQ-dependent sugar dehydrogenase and cytochrome domains. Although this enzyme shows low amino acid sequence homology with known PQQ-dependent enzymes, it strongly binds PQQ and shows PQQ-dependent activity. BLAST search uncovered the existence of many genes encoding homologous proteins in bacteria, archaea, amoebozoa, and fungi, and phylogenetic analysis suggested that these quinoproteins may be members of a new family that is widely distributed not only in prokaryotes, but also in eukaryotes.
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- 2014
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8. The putative endoglucanase PcGH61D from Phanerochaete chrysosporium is a metal-dependent oxidative enzyme that cleaves cellulose.
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Bjørge Westereng, Takuya Ishida, Gustav Vaaje-Kolstad, Miao Wu, Vincent G H Eijsink, Kiyohiko Igarashi, Masahiro Samejima, Jerry Ståhlberg, Svein J Horn, and Mats Sandgren
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Medicine ,Science - Abstract
Many fungi growing on plant biomass produce proteins currently classified as glycoside hydrolase family 61 (GH61), some of which are known to act synergistically with cellulases. In this study we show that PcGH61D, the gene product of an open reading frame in the genome of Phanerochaete chrysosporium, is an enzyme that cleaves cellulose using a metal-dependent oxidative mechanism that leads to generation of aldonic acids. The activity of this enzyme and its beneficial effect on the efficiency of classical cellulases are stimulated by the presence of electron donors. Experiments with reduced cellulose confirmed the oxidative nature of the reaction catalyzed by PcGH61D and indicated that the enzyme may be capable of penetrating into the substrate. Considering the abundance of GH61-encoding genes in fungi and genes encoding their functional bacterial homologues currently classified as carbohydrate binding modules family 33 (CBM33), this enzyme activity is likely to turn out as a major determinant of microbial biomass-degrading efficiency.
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- 2011
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9. Blood supply to the caudate lobe of the liver from the right inferior phrenic artery: observation by cone-beam computed tomography during arteriography
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Shiro Miyayama, Naoko Sakuragawa, Natsuki Sugimori, Rie Ikeda, Masashi Yamashiro, and Takuya Ishida
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medicine.medical_specialty ,Cone beam computed tomography ,Carcinoma, Hepatocellular ,Urology ,medicine.medical_treatment ,Inferior vena cava ,030218 nuclear medicine & medical imaging ,Caudate lobe of liver ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Chemoembolization, Therapeutic ,Retrospective Studies ,Radiological and Ultrasound Technology ,business.industry ,Liver Neoplasms ,Angiography ,Gastroenterology ,Cone-Beam Computed Tomography ,Hepatology ,medicine.disease ,Lobe ,medicine.anatomical_structure ,Liver ,medicine.vein ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Ligament ,Hepatectomy ,Tomography, X-Ray Computed ,Nuclear medicine ,business - Abstract
To retrospectively evaluate blood supply to the caudate lobe of the liver from the right inferior phrenic artery (RIPA) using cone-beam computed tomography during arteriography (CBCTA-RIPA). CBCTA-RIPA examinations during transarterial chemoembolization (TACE) for hepatocellular carcinoma (HCC) were collected from 2448 procedures in 787 patients. The exclusion criteria were (1) major artifacts, (2) TACE of hepatic arterial branches before performing CBCTA-RIPA, and (3) repeated CBCTA-RIPA studies in the same patient. Blood supply to three subsegments, the Spiegel lobe (SP), paracaval, and caudate process, was evaluated on CBCTA-RIPA images. The origins and routes of branches supplying the caudate lobe were also evaluated by three-dimensional vessel-tracking software. Forty-seven CBCTA-RIPA examinations in 47 patients (38 with a history of TACE [repeated TACE group] and nine without it [initial TACE group]) were eligible, including five who had previously undergone hepatectomy. Seven had caudate lobe HCCs. Blood supply to the caudate lobe was demonstrated in 21 (44.7%) patients: in 50% (19/38) and 22.2% (2/9) of the repeated and initial TACE groups, respectively. The caudate arteries had previously been embolized in eight patients, the RIPA branch in three, and both in one. Forty-one proximal branches mainly supplied the dorsal part of the SP. All branches but five reached there through the inferior vena cava (IVC) ligament. The RIPA supplied eight of nine caudate lobe HCCs, totally (n = 7) or partially (n = 1). The proximal RIPA branches mainly supply the dorsal SP through the IVC ligament, especially in the repeated TACE group.
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- 2020
10. Intraoperative Bile Duct Disruption Treated Using Intrahepatic Biliary Ablation with Ethanol: A Report of Two Cases
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Natsuki Sugimori, Masashi Yamashiro, Naoko Sakuragawa, Takuro Terada, Shiro Miyayama, Takuya Ishida, and Rie Ikeda
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medicine.medical_specialty ,chemistry.chemical_compound ,medicine.anatomical_structure ,Ethanol ,chemistry ,business.industry ,Bile duct ,Iodized oil ,medicine.medical_treatment ,Urology ,Medicine ,business ,Ablation - Published
- 2020
11. Transarterial Interventional Therapy for Non-functioning Hemodialysis Access
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Rie Ikeda, Tamayo Kato, Shiro Miyayama, Natsuki Sugimori, Masashi Yamashiro, Takuya Ishida, Yasuyuki Ushiogi, Yasutaka Kamikawa, and Naoko Sakuragawa
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Interventional therapy ,medicine.medical_specialty ,Thrombotic occlusion ,business.industry ,Transarterial approach ,Medicine ,business ,Hemodialysis access ,Surgery - Published
- 2020
12. Sulfur accumulation in soil in a forested watershed historically exposed to air pollution in central Japan
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Masamichi Takahashi, Masato Kamisako, Michiru Yamashita, Yoshiaki Ito, Hiroyuki Sase, Chisato Takenaka, Sei Fukushima, Naoyuki Yamashita, Akifumi Sugiyama, Takuya Ishida, Toko Tanikawa, and Akihiro Imaya
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Air pollution ,Soil Science ,chemistry.chemical_element ,Mineralization (soil science) ,medicine.disease_cause ,Sulfur ,Pedogenesis ,δ34S ,chemistry ,Environmental chemistry ,Soil water ,medicine ,Environmental science ,Soil horizon ,Surface water - Abstract
Forest soils that have accumulated anthropogenic sulfur (S) as a result of historical air pollution may gradually or suddenly release inorganic sulfate either by desorption of inorganic sulfate or by mineralization of organic S, even after mitigation of previous air pollution. The release of inorganic sulfate from soil to surface water leads directly to watershed acidification. Thus, whether the organic S compounds that have accumulated in soils are resistant to microbial activity, due to formation of organo-mineral complexes, is essential information for management of forest soil ecosystem services. A nationwide air pollution monitoring survey by the Japanese Ministry of the Environment identified acidification of both surface water and soil in the Lake Ijira watershed of central Japan, presumably sourced from the Chukyo Industrial Area which lies upwind of the watershed. We compared the amount of S accumulated in the Ijira soils (Dystrudepts) with that in comparable soils in other watersheds in central Japan that were less affected by industrial air pollution. To determine how much mineral-associated organic S had accumulated in the Ijira soils, we measured pyrophosphate- extractable S. We used high-resolution x-ray fluorescence (HRXRF) spectroscopy with a double-crystal type high energy resolution spectrometer to estimate the oxidation states of both mineral-associated and free organic S. We also measured S isotope compositions to explore S dynamics in the soils. We found that more S had accumulated in the Ijira soils than in the comparable soils from other watersheds in central Japan. Concentrations of total S in the Ijira soils varied among soil horizons, reaching more than 1000 mg S kg−1 in some horizons. Mineral-associated organic S accounted for more than half of the total accumulated S. High-resolution x-ray fluorescence spectrum analyses showed that mineral-associated organic S was retained at both high and low oxidation states. The δ34S values of total S in the deeper horizons tended to be higher than those of the uppermost horizons of the Ijira soils and correlated positively with concentrations of extractable Al and Fe oxides (which characterize pedogenic minerals). Our results suggest that about half of total accumulated S in Ijira soils is stabilized in pedogenic minerals and the remainder comprises free organic S and adsorbed inorganic sulfates. There might have been repetitive mobilization and immobilization of S and the degraded organic S might have greater affinity with pedogenic minerals. Further, the ratio of total C to mineral-associated organic S correlated negatively with concentrations of extractable Al and Fe oxides, suggesting that organic S is more resistant to mineralization than organic C because S is preferentially associated with the pedogenic minerals. Monitoring of the chemical states of S compounds in soils historically exposed to air pollution is recommended to assist in prediction of the fate of the S compounds that have accumulated in them.
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- 2022
13. Increased large-scale inter-network connectivity in relation to impulsivity in Parkinson’s disease
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Yoshiaki Nakayama, Tomohiro Donishi, Yoshiki Kaneoke, Mayumi Sakata, Masaki Terada, Ken-ya Murata, Jinsoo Koh, Yasuhiro Hiwatani, Masaaki Yasui, Hidefumi Ito, Masaya Hironishi, Takuya Ishida, and Hiroshi Ishiguchi
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Male ,0301 basic medicine ,medicine.medical_specialty ,Parkinson's disease ,lcsh:Medicine ,Audiology ,Impulsivity ,Basal Ganglia ,Article ,03 medical and health sciences ,0302 clinical medicine ,Barratt Impulsiveness Scale ,Humans ,Medicine ,Attention ,lcsh:Science ,Anterior cingulate cortex ,Aged ,Brain Mapping ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,Functional connectivity ,lcsh:R ,Brain ,Parkinson Disease ,Cognition ,Middle Aged ,Network connectivity ,medicine.disease ,Magnetic Resonance Imaging ,030104 developmental biology ,medicine.anatomical_structure ,Neurology ,Impulsive Behavior ,Disease Progression ,Female ,lcsh:Q ,Nerve Net ,medicine.symptom ,business ,Functional magnetic resonance imaging ,030217 neurology & neurosurgery ,Neuroscience - Abstract
Impulsivity is a neuropsychiatric feature of Parkinson’s disease (PD). We investigated the pathophysiology of impulsivity in PD using resting-state functional magnetic resonance imaging (rs-fMRI). We investigated 45 patients with idiopathic PD and 21 healthy controls. Based on Barratt Impulsiveness Scale (BIS-11) score, PD patients were classified as higher (PD-HI) or lower impulsivity (PD-LI). Functional connectivity (FC) between various large-scale brain networks were analysed using the CONN toolbox. FC between the right frontoparietal network (FPN) and medial visual network (MVN) was significantly higher in PD-HI patients than PD-LI patients (false discovery rate [FDR]-adjusted p = 0.0315). FC between the right FPN and MVN had a significant positive correlation with total BIS-11 score (FDR-adjusted p = 0.010) and the attentional impulsivity (FDR-adjusted p = 0.046) and non-planning impulsivity subscale scores (FDR-adjusted p = 0.018). On the other hand, motor impulsivity subscale score had a significant negative correlation with the FC between the default-mode and salience networks (right supramarginal gyrus, FDR-adjusted p = 0.018; anterior cingulate cortex, FDR-adjusted p = 0.027); this trend was observed in healthy controls. The attentional and non-planning impulsivity, regarded as ‘cognitive’ impulsivity, may be associated with dysfunction in integration of perceptual information and flexible cognitive control in PD.
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- 2020
14. Converging Resting State Networks Unravels Potential Remote Effects of Transcranial Magnetic Stimulation for Major Depression
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Werner Strik, Yosuke Morishima, Thomas Dierks, and Takuya Ishida
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lcsh:RC435-571 ,medicine.medical_treatment ,Ventromedial prefrontal cortex ,610 Medicine & health ,resting-state functional magnetic resonance imaging (fMRI) ,Amygdala ,behavioral disciplines and activities ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Psychiatry ,mental disorders ,medicine ,repetitive transcranial magnetic stimulation (rTMS) ,Original Research ,Psychiatry ,Resting state fMRI ,major depressive disorder ,business.industry ,functional connectivity ,Dynamic causal modelling ,030227 psychiatry ,Dorsolateral prefrontal cortex ,Transcranial magnetic stimulation ,Psychiatry and Mental health ,medicine.anatomical_structure ,nervous system ,Brain stimulation ,dynamic causal modeling (DCM) ,Granger causality analysis (GCA) ,business ,Neuroscience ,Insula ,030217 neurology & neurosurgery ,psychological phenomena and processes - Abstract
Despite being a commonly used protocol to treat major depressive disorder (MDD), the underlying mechanism of repetitive transcranial magnetic stimulation (rTMS) on dorsolateral prefrontal cortex (DLPFC) remains unclear. In the current study, we investigated the resting-state fMRI data of 100 healthy subjects by exploring three overlapping functional networks associated with the psychopathologically MDD-related areas (the nucleus accumbens, amygdala, and ventromedial prefrontal cortex). Our results showed that these networks converged at the bilateral DLPFC, which suggested that rTMS over DLPFC might improve MDD by remotely modulating the MDD-related areas synergistically. Additionally, they functionally converged at the DMPFC and bilateral insula which are known to be associated with MDD. These two areas could also be potential targets for rTMS treatment. Dynamic causal modelling (DCM) and Granger causality analysis (GCA) revealed that all pairwise connections among bilateral DLPFC, DMPFC, bilateral insula, and three psychopathologically MDD-related areas contained significant causality. The DCM results also suggested that most of the functional interactions between MDD-related areas and bilateral DLPFC, DMPFC, and bilateral insula can predominantly be explained by the effective connectivity from the psychopathologically MDD-related areas to the rTMS stimulation sites. Finally, we found the conventional functional connectivity to be a more representative measure to obtain connectivity parameters compared to GCA and DCM analysis. Our research helped inspecting the convergence of the functional networks related to a psychiatry disorder. The results identified potential targets for brain stimulation treatment and contributed to the optimization of patient-specific brain stimulation protocols.
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- 2020
15. Widespread abnormalities in white matter integrity and their relationship with duration of illness in temporal lobe epilepsy
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Takuya Ishida, Shinichi Yamada, Masaki Terada, Tomikimi Tsuji, Satoshi Ukai, Shun Takahashi, Kazuhiro Shinosaki, Yuji Ohoshi, and Kumi Tsuda
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medicine.medical_specialty ,Duration of illness ,Audiology ,behavioral disciplines and activities ,030218 nuclear medicine & medical imaging ,Temporal lobe ,White matter ,Correlation ,03 medical and health sciences ,Epilepsy ,0302 clinical medicine ,Fractional anisotropy ,Medicine ,In patient ,Tract‐based spatial statistics ,Temporal lobe epilepsy ,business.industry ,Radial diffusivity ,medicine.disease ,Diffusion tensor imaging ,medicine.anatomical_structure ,nervous system ,Neurology ,Full‐length Original Research ,Neurology (clinical) ,business ,psychological phenomena and processes ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
Summary Objective Elucidation of abnormal connections throughout the whole brain is necessary to understand temporal lobe epilepsy (TLE). We examined abnormalities in whole‐brain white matter integrity and their relationship with duration of illness in patients with TLE. Methods The subjects were 15 patients with TLE and 17 healthy controls. Mean duration of illness in the TLE group was 21.6 years. Tract‐based spatial statistics (TBSS) were used for diffusion tensor imaging (DTI) analysis. Four diffusion tensor metrics, that is, fractional anisotropy (FA), mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD) were calculated and then examined for differences between the TLE and healthy control groups. We also examined for correlations between DTI parameters and duration of illness in the TLE group. Results In the TLE group, compared with the healthy control group, FA was reduced, and MD and RD were increased, not only in the limbic and temporal lobe regions and their directly connecting regions in both hemispheres, but also in remote white matter regions. Duration of illness showed a significant negative correlation with mean whole‐brain FA and a significant positive correlation with both mean whole‐brain MD and RD. Brain regions showing correlation between disease duration and DTI metrics also extended to the limbic area and its connecting regions, and to remote white matter regions. Significance The results of this study suggest that widespread abnormalities in white matter integrity in patients with TLE are associated with long‐term disease.
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- 2018
16. Elucidating the aberrant brain regions in bipolar disorder using T1-weighted/T2-weighted magnetic resonance ratio images
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Tomohiro Donishi, Yoshiki Kaneoke, Takuya Ishida, Jun Iwatani, Masaki Terada, Kazuhiro Shinosaki, Shinichi Yamada, Shun Takahashi, and Satoshi Ukai
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Cerebellum ,Bipolar Disorder ,Internal capsule ,Neuroscience (miscellaneous) ,Corpus callosum ,Corpus Callosum ,White matter ,03 medical and health sciences ,0302 clinical medicine ,Internal Capsule ,Middle cerebellar peduncle ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Gray Matter ,medicine.diagnostic_test ,business.industry ,Brain ,Magnetic resonance imaging ,Anatomy ,Middle Aged ,White Matter ,030227 psychiatry ,Psychiatry and Mental health ,Diffusion Tensor Imaging ,medicine.anatomical_structure ,nervous system ,Case-Control Studies ,Brain size ,Female ,business ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
Although diffusion tensor imaging (DTI) have revealed brain abnormalities in bipolar disorder (BD) subjects, DTI methods might not detect disease-related abnormalities in the white matter (WM) where nerve fibers are crossing. We investigated BD myelin-related abnormal brain regions in both gray matter and WM for 29 BD and 33 healthy control (HC) participants using T1-weighted (T1w)/T2-weighted (T2w) ratio images that increase myelin-related contrast irrespective of nerve fiber orientation. To check effect of the brain volume, the results were compared with those of voxel-based morphometry (VBM). We found significantly lower T1w/T2w signal intensity in broad WM regions in BD subjects, including the corpus callosum, corona radiata, internal capsule, middle cerebellar peduncle and cerebellum. Regional volume reduction was found in the WM bilateral posterior thalami and retrolenticular part of the internal capsules of BD subjects. We also performed tract-based spatial statistics (TBSS) in 25 BD and 24 HC participants and compared those for the T1w/T2w ratio images. Both methods detected the BD corpus callosum abnormality. Further, the ratio images detected the corona radiata and the cerebellar abnormality in BD. These results suggest that T1w/T2w ratio image analysis could take a complementary role with the DTI method in elucidating myelin-related abnormalities in BD.
- Published
- 2017
17. Widespread white matter microstructural abnormalities and cognitive impairment in schizophrenia, bipolar disorder, and major depressive disorder: Tract-based spatial statistics study
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Yuji Ohoshi, Shinichi Yamada, Shun Takahashi, Satoshi Ukai, Tomikimi Tsuji, Takuya Ishida, Masaki Terada, and Kazuhiro Shinosaki
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medicine.medical_specialty ,business.industry ,Neuroscience (miscellaneous) ,Tract based spatial statistics ,medicine.disease ,White matter ,Psychiatry and Mental health ,medicine.anatomical_structure ,Schizophrenia ,medicine ,Major depressive disorder ,Radiology, Nuclear Medicine and imaging ,Bipolar disorder ,business ,Cognitive impairment ,Psychiatry - Published
- 2019
18. Microstructural abnormalities in callosal fibers and their relationship with cognitive function in schizophrenia: A tract‐specific analysis study
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Tomikimi Tsuji, Takuya Ishida, Satoshi Ukai, Shinichi Yamada, Kumi Tsuda, Masaki Terada, Shun Takahashi, Yuji Ohoshi, and Kazuhiro Shinosaki
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Adult ,Male ,cognition ,medicine.medical_specialty ,Neuropsychological Tests ,Audiology ,Corpus callosum ,050105 experimental psychology ,lcsh:RC321-571 ,Temporal lobe ,corpus callosum ,White matter ,Executive Function ,03 medical and health sciences ,Behavioral Neuroscience ,0302 clinical medicine ,Fractional anisotropy ,Tower of London test ,medicine ,Humans ,magnetic resonance imaging ,0501 psychology and cognitive sciences ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Original Research ,Positive and Negative Syndrome Scale ,business.industry ,05 social sciences ,Middle Aged ,diffusion tensor imaging ,medicine.disease ,White Matter ,schizophrenia ,medicine.anatomical_structure ,Schizophrenia ,Anisotropy ,Female ,Schizophrenic Psychology ,business ,030217 neurology & neurosurgery ,Diffusion MRI - Abstract
Introduction The corpus callosum serves the essential role of relaying cognitive information between the homologous regions in the left and the right hemispheres of the brain. Cognitive impairment is a core dysfunction of schizophrenia, but much of its pathophysiology is unknown. The aim of this study was to elucidate the association between microstructural abnormalities of the corpus callosum and cognitive dysfunction in schizophrenia. Methods We examined stepwise multiple regression analysis to investigate the relationship of the fractional anisotropy (FA) of callosal fibers in each segment with z‐scores of each brief assessment of cognition in schizophrenia subtest and cognitive composite score in all subjects (19 patients with schizophrenia [SZ group] and 19 healthy controls [HC group]). Callosal fibers were separated into seven segments based on their cortical projection using tract‐specific analysis of diffusion tensor imaging. Results The FA of callosal fibers in the temporal segment was significantly associated with z‐scores of token motor test, Tower of London test, and the composite score. In the SZ group, the FA of callosal fibers in the temporal segment was significantly associated with the z‐score of the Tower of London test. In addition, the FA of callosal fibers in temporal segment showed significant negative association with the positive and negative syndrome scale negative score in the SZ group. Compared to the HC group, the FA in temporal segment was significantly decreased in the SZ group. Conclusion Our results suggest that microstructural abnormalities in the callosal white matter fibers connecting bilateral temporal lobe cortices contribute to poor executive function and severe negative symptom in patients with schizophrenia.
- Published
- 2019
19. Relationships between oxycodone pharmacokinetics, central symptoms, and serum interleukin-6 in cachectic cancer patients
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Junichi Kawakami, Takuya Ishida, Hikaru Sato, and Takafumi Naito
- Subjects
Male ,Cachexia ,Interleukin-1beta ,Disorders of Excessive Somnolence ,Pharmacology ,030226 pharmacology & pharmacy ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,Neoplasms ,medicine ,Cytochrome P-450 CYP3A ,Humans ,Pharmacology (medical) ,Interleukin 6 ,Aged ,biology ,CYP3A4 ,Depression ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,business.industry ,Delirium ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Analgesics, Opioid ,Cytochrome p450 enzyme ,Delayed-Action Preparations ,030220 oncology & carcinogenesis ,biology.protein ,Female ,business ,Oxycodone ,medicine.drug - Abstract
Elevated serum proinflammatory cytokines are associated with the reduction of cytochrome P450 enzyme (CYP) activity. This study aimed to evaluate the oxycodone pharmacokinetics, central symptoms, and serum proinflammatory cytokines based on cachexia stage in cancer patients.Forty-seven cancer patients receiving extended-release oxycodone were enrolled. Predose plasma concentrations of oxycodone and its metabolites were normalized with the daily dose and body weight. The central symptoms and serum level of proinflammatory cytokines were investigated at each cachexia stage.The plasma concentrations of oxycodone in patients with cachexia and refractory cachexia were significantly higher than that in patients with precachexia. The metabolic ratio to noroxycodone in patients with cachexia was significantly lower than that in patients with precachexia. The patients with a higher cachexia stage had a higher serum level of interleukin-6 (IL-6), but not tumor necrosis factor-α and interleukin-1β. The serum IL-6 level was correlated with the plasma concentration of oxycodone and inversely with the metabolic ratio to noroxycodone. The incidence of somnolence was not associated with the plasma oxycodone concentration. In contrast, the cachexia stage and its associated serum IL-6 level were correlated with the incidence of somnolence.Cancer cachexia raised the plasma exposure of oxycodone through the reduction of CYP3A metabolic pathway. The reduction of CYP3A in cachectic cancer patients was associated with an elevation of serum IL-6. Although cachectic cancer patients with higher serum IL-6 levels had the symptom of somnolence, the alterations in oxycodone pharmacokinetics were not related to the incidence of symptom.
- Published
- 2016
20. CYP3A activity based on plasma 4β-hydroxycholesterol during the early postpartum period has an effect on the plasma disposition of amlodipine
- Author
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Hiroaki Itoh, Takuya Ishida, Junichi Kawakami, Shuhei Deguchi, Naohiro Kanayama, Naoko Kubono, Masahisa Sugihara, and Takafumi Naito
- Subjects
Adult ,medicine.medical_specialty ,CYP3A ,Pharmaceutical Science ,Substrate Specificity ,chemistry.chemical_compound ,Pharmacokinetics ,Pregnancy ,Internal medicine ,polycyclic compounds ,Cytochrome P-450 CYP3A ,Humans ,Medicine ,Pharmacology (medical) ,Amlodipine ,Antihypertensive Agents ,Biotransformation ,Pharmacology ,CYP3A4 ,business.industry ,Cholesterol ,Postpartum Period ,Hypertension, Pregnancy-Induced ,Disposition ,Calcium Channel Blockers ,medicine.disease ,Hydroxycholesterols ,Up-Regulation ,Endocrinology ,chemistry ,Female ,lipids (amino acids, peptides, and proteins) ,business ,Biomarkers ,Postpartum period ,medicine.drug - Abstract
This study aimed to evaluate plasma 4β-hydroxycholesterol as an endogenous marker of CYP3A4/5 activity in early postpartum women and its impact on the plasma disposition of amlodipine. Twenty-seven early postpartum women treated with amlodipine for pregnancy-induced hypertension were enrolled. The plasma concentration of 4β-hydroxycholesterol and its ratio to cholesterol in postpartum and in non-perinatal women were evaluated. The predose plasma concentration of amlodipine was determined at steady state. The medians of the plasma 4β-hydroxycholesterol concentration at day 0-3 and 8-21 after delivery were 146 and 161 ng/mL, respectively. No significant difference was observed in the plasma concentration of 4β-hydroxycholesterol between the postpartum periods. The plasma concentration of 4β-hydroxycholesterol and its ratio to cholesterol in postpartum women were significantly higher than those in non-perinatal women. A large individual variability was observed in the dose-normalized plasma concentration of amlodipine in early postpartum women. A weak negative correlation was observed between the dose-normalized plasma concentration of amlodipine and the plasma concentration of 4β-hydroxycholesterol. In conclusion, early postpartum women possessed higher CYP3A activity based on plasma 4β-hydroxycholesterol and had a large pharmacokinetic variability in amlodipine. CYP3A activity during the early postpartum period had an effect on the plasma disposition of amlodipine.
- Published
- 2015
21. Quantitative Reconstruction of Sulfur Deposition Using a Mixing Model Based on Sulfur Isotope Ratios in Tree Rings
- Author
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Chisato Takenaka, Ichiro Tayasu, and Takuya Ishida
- Subjects
Pollution ,Hydrology ,Environmental Engineering ,Isotope ,Chemistry ,media_common.quotation_subject ,Air pollution ,chemistry.chemical_element ,Management, Monitoring, Policy and Law ,Atmospheric sciences ,medicine.disease_cause ,Sulfur ,Deposition (aerosol physics) ,Forest ecology ,medicine ,Ecosystem ,Terrestrial ecosystem ,Waste Management and Disposal ,Water Science and Technology ,media_common - Abstract
Quantification of sulfur (S) deposition is critical to deciphering the environmental archive of S in terrestrial ecosystems. Here we propose a mixing model that quantifies S deposition based on the S isotope ratio (δS) in tree rings. We collected samples from Japanese cedar ( D. Don) stumps from two sites: one near Yokkaichi City (YOK), which is well known for having the heaviest S air pollution in the world, and one at Inabu-cho (INA) in central Japan, which has been much less affected by air pollution. The δS profiles at both sites are consistent with S air pollution and contributions of anthropogenic S. The minimum value in YOK is lower than the δS values of anthropogenic S or any other possible source. Because the δS in the tree rings is affected by fractionation in the forest ecosystems, we used a mixing model to account for the isotope effects and to distinguish the sources of S. Based on the model results, we infer that the peak of S emissions at YOK occurred sometime between the late 1960s and early 1970s (489 mmol m yr). This estimated value is comparable with the highest reported values in Europe. This is the first quantitative estimate of anthropogenic input of S in forest systems based on δS in tree rings. Our results suggest that tree ring data can be used when monitoring stations of atmospheric S are lacking and that estimates of S deposition using δS in tree rings will advance our understanding of the local-scale S dynamics and the effect of human activities on it.
- Published
- 2015
22. Simultaneous determination of erlotinib and its isomeric major metabolites in human plasma using isocratic liquid chromatography-tandem mass spectrometry and its clinical application
- Author
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Takafumi Naito, Junichi Kawakami, and Takuya Ishida
- Subjects
Pharmacology ,Analyte ,Chromatography ,Chemistry ,Calibration curve ,Clinical Biochemistry ,General Medicine ,Mass spectrometry ,Biochemistry ,respiratory tract diseases ,Analytical Chemistry ,chemistry.chemical_compound ,Liquid chromatography–mass spectrometry ,Human plasma ,Drug Discovery ,medicine ,Particle size ,Erlotinib ,Acetonitrile ,Molecular Biology ,medicine.drug - Abstract
This study developed a method for the simultaneous determination of erlotinib and its isomeric major metabolites, OSI-413 and OSI-420, in human plasma using an isocratic liquid chromatography–tandem mass spectrometry. Plasma specimens deproteinized with acetonitrile were separated using a 3-µm particle size octadecylsilyl column. The m/z values of the precursor and product ions for the analytes were as follows: erlotinib, 394.2/278.2; and OSI-413 and OSI-420, 380.2/278.2. The total run time was 21 min and no peaks interfering with the analytes and internal standard (d6-erlotinib) in human plasma were observed. The calibration curves of erlotinib, OSI-413 and OSI-420 were linear over the concentration ranges of 10–3000, 2–500 and 2–100 ng/mL, respectively. The pretreatment recovery ratios were >86.1%. The intra- and inter-assay precisions and accuracies were
- Published
- 2014
23. Plasmon-induced charge separation: chemistry and wide applications
- Author
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Takuya Ishida, Tetsu Tatsuma, and Hiroyasu Nishi
- Subjects
Chemistry ,Charge separation ,business.industry ,Nanotechnology ,02 engineering and technology ,General Chemistry ,Electrostatic induction ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Photovoltaics ,medicine ,medicine.symptom ,0210 nano-technology ,business ,Plasmon ,Confusion - Abstract
Electrons transfer from plasmonic nanoparticles to semiconductors by exploiting the energy of light, and this effect is applied to photovoltaics, photocatalysis, sensing, photochromisms, photoswitchable functionalities and nanofabrications., Recent development of nanoplasmonics has stimulated chemists to utilize plasmonic nanomaterials for efficient and distinctive photochemical applications, and physicists to boldly go inside the “wet” chemistry world. The discovery of plasmon-induced charge separation (PICS) has even accelerated these trends. On the other hand, some confusion is found in discussions about PICS. In this perspective, we focus on differences between PICS and some other phenomena such as co-catalysis effect and plasmonic nanoantenna effect. In addition, materials and nanostructures suitable for PICS are shown, and characteristics and features unique to PICS are documented. Although it is well known that PICS has been applied to photovoltaics and photocatalysis, here light is shed on other applications that take better advantage of PICS, such as chemical sensing and biosensing, various photochromisms, photoswitchable functionalities and nanoscale photofabrication.
- Published
- 2017
24. The GH26 β-mannanase RsMan26H from a symbiotic protist of the termite Reticulitermes speratus is an endo-processive mannobiohydrolase: Heterologous expression and characterization
- Author
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Akihiko Nakamura, Manabu Arioka, Kiyohiko Igarashi, Katsuhiko Kitamoto, Shigeharu Moriya, Hikaru Tsukagoshi, Takuya Ishida, Masahiro Samejima, and Masato Otagiri
- Subjects
Protozoan Proteins ,Biophysics ,Gene Expression ,Oligosaccharides ,Isoptera ,Parabasalidea ,Biology ,medicine.disease_cause ,Polysaccharide ,Biochemistry ,Pichia ,Microbiology ,Pichia pastoris ,Mannans ,Polysaccharides ,parasitic diseases ,medicine ,Mannobiose ,Animals ,Glycoside hydrolase ,Symbiosis ,Molecular Biology ,chemistry.chemical_classification ,beta-Mannosidase ,Protist ,Cell Biology ,Processivity ,biology.organism_classification ,Kinetics ,Enzyme ,chemistry ,Heterologous expression - Abstract
Symbiotic protists in the gut of termites are prominent natural resources for enzymes involved in lignocellulose degradation. Here we report expression, purification, and biochemical characterization of a glycoside hydrolase family 26 mannanase RsMan26H from the symbiotic protist of the lower termite, Reticulitermes speratus. Biochemical analysis of RsMan26H demonstrates that this enzyme is an endo-processive mannobiohydrolase producing mannobiose from oligo- and polysaccharides, followed by a minor accumulation of oligosaccharides larger than mannobiose. To our knowledge, this is the first report describing the unique mannobiohydrolase enzyme from the eukaryotic origin.
- Published
- 2014
25. Sarcomatoid Carcinoma of the Urinary Bladder
- Author
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Takuya Ishida, Naohiro Kamoda, Isao Kuroda, Kenji Shimodaira, Masaaki Tachibana, and Teiichiro Aoyagi
- Subjects
medicine.medical_specialty ,Chemotherapy ,Urinary bladder ,Bladder cancer ,business.industry ,medicine.medical_treatment ,Urology ,Cancer ,General Medicine ,medicine.disease ,Metastasis ,Cystectomy ,medicine.anatomical_structure ,Carcinoma ,Medicine ,business ,Sarcomatoid carcinoma - Abstract
The patient was a 62-year-old female with bladder carcinoma. TUR-BT was performed in March 2010, and the pathological finding was UC, G2 > G1, pTa. However, the cancer recurred in the urinary bladder 3 months after TUR-BT. Radical cystectomy was performed, and the disease was considered to have been cured, but metastasis developed in the skin, lung, liver, and bone several months after surgery. Chemotherapy was ineffective, and the patient died. On pathological examination at the time of radical cystectomy, the lesion was sarcomatoid carcinoma of the urinary bladder. Although the prognosis associated with this carcinoma is known to be poor, the possibility of underestimation on preoperative staging cannot be ruled out from the cause, in addition to the insufficiency of the current therapeutic strategy.
- Published
- 2014
26. Cancer Cachexia Raises the Plasma Concentration of Oxymorphone Through the Reduction of CYP3A But Not CYP2D6 in Oxycodone-Treated Patients
- Author
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Masaki Tashiro, Takafumi Naito, Junichi Kawakami, Kazunori Ohnishi, and Takuya Ishida
- Subjects
Pharmacology ,business.industry ,Incidence (epidemiology) ,medicine.disease ,Cachexia ,Pharmacokinetics ,Oxymorphone ,Anesthesia ,Vomiting ,Medicine ,Pharmacology (medical) ,medicine.symptom ,business ,Cancer pain ,Adverse effect ,Oxycodone ,medicine.drug - Abstract
This study evaluated the plasma concentrations of oxycodone and its demethylates and opioid-induced adverse effects based on cachexia stage in cancer patients receiving oxycodone. Seventy patients receiving oxycodone for cancer pain were enrolled. Cachexia was evaluated using the Glasgow Prognostic Score (GPS). Predose plasma concentrations of oxycodone, oxymorphone, and noroxycodone were determined at the titration dose. Opioid-induced adverse effects were monitored for 2 weeks after the titration. Plasma concentrations of oxycodone and oxymorphone but not noroxycodone in patients with a GPS of 2 were significantly higher than that with a GPS of 0. The metabolic ratios of noroxycodone but not oxymorphone to oxycodone in patients with a GPS of 1 and 2 were significantly lower than in those with a GPS of 0. A higher GPS was associated with a higher incidence of somnolence, while the GPS did not affect the incidence of vomiting. Plasma concentrations of oxycodone and oxymorphone were not associated with the incidence of adverse effects. In conclusion, cancer cachexia raised the plasma exposures of oxycodone and oxymorphone through the reduction of CYP3A but not CYP2D6. Although the cachexia elevated the incidence of somnolence, alterations in their pharmacokinetics were not associated with the incidence.
- Published
- 2013
27. Neuropsychological and functional correlates of clock-drawing test in elderly institutionalized patients with schizophrenia
- Author
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Akiko Kashima, Takuya Ishida, Chizuru Morioka, Shutaro Nakaaki, Mutsumi Kohigashi, Keisuke Shibata, Masaki Kitabayashi, Kenji Fukui, Jin Narumoto, Naosuke Tani, Masaru Mimura, Yurinosuke Kitabayashi, and Aiko Okamura
- Subjects
education.field_of_study ,medicine.medical_specialty ,Psychometrics ,Positive and Negative Syndrome Scale ,Population ,Neuropsychology ,Cognition ,medicine.disease ,Cognitive test ,Psychiatry and Mental health ,Schizophrenia ,medicine ,Dementia ,Geriatrics and Gerontology ,education ,Psychiatry ,Psychology ,Gerontology ,Clinical psychology - Abstract
Background: There has been a growing need for a cognitive assessment tool that can be used for older adults with schizophrenia in clinical settings. The clock-drawing test (CDT) is a brief cognitive test that covers a wide range of cognitive function. Although it is widely used to assess patients with dementia, limited data are available on its usefulness in older patients with schizophrenia. Thus, we investigated the psychometric properties of the CDT and their relationship with life functions to examine the test's usefulness for assessing cognitive function in older adults with schizophrenia. Methods: Seventy-three older adults with chronic schizophrenia who had been hospitalized for over 1 year participated in the study. We adopted the executive clock-drawing task for administration and scoring of the CDT, which consists of free-drawn and copy conditions. The Mini-Mental State Examination and the Brief Assessment of Cognition in Schizophrenia were administered. Symptom severity and life functions were assessed with the Positive and Negative Syndrome Scale and the Life Skills Profile, respectively. Results: Both free-drawn and copy scores significantly correlated with the Mini-Mental State Examination score and the Brief Assessment of Cognition in Schizophrenia composite score. These scores also significantly correlated with symptom severity and length of current hospitalization. Stepwise regression analysis showed that only the copy score, together with symptom severity, predicted the Life Skills Profile score. Conclusions: The CDT can assess cognitive function in older adults with schizophrenia. Moreover, CDT performance is associated with life functions independent from other clinical variables. These results suggest that the CDT is a useful cognitive assessment tool for this population.
- Published
- 2012
28. Trends in Non-prescription Drug Recalls in Japan
- Author
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Junichi Kawakami, Chikoto Yamamoto, Takuya Ishida, Takafumi Naito, and Takashi Osawa
- Subjects
Pharmacology ,Drug ,medicine.medical_specialty ,Time Factors ,Drug Industry ,business.industry ,media_common.quotation_subject ,Pharmaceutical Science ,Nonprescription Drugs ,Self Medication ,Legislation, Drug ,Human health ,Government Agencies ,Japan ,Topical agents ,Family medicine ,Therapeutic Categories ,medicine ,Non prescription ,Christian ministry ,Drug Recalls ,business ,Drug recall ,media_common - Abstract
Recalls of non-prescription drugs can contribute to preventing harm to human health, however, they also interrupt the supply of medicines to the market. The aim of the present study was to investigate the trends in non-prescription drug recalls in Japan. Class I, II, and III recalls reported from April 2009 to March 2014 were obtained from the websites of the Ministry of Health, Labour and Welfare and the Pharmaceuticals and Medical Devices Agency. Each drug recall was classified according to year, dosage form, therapeutic category, and reasons for the recall. The trends over the 5 year period were assessed for each class. A total of 220 recalls were reported in the 5-year study period. The numbers of drug recalls were 21, 16, 80, 58, and 45 in 2009, 2010, 2011, 2012, and 2013, respectively. The drugs recalled consisted of 177 internal medications, 35 topical agents, and 8 others. Drug recalls were observed in 12 therapeutic categories of drug effects. The largest number of recalls was for Chinese herbal medicines and crude drugs. Of all the drug recalls in 2011, Chinese herbal medicines and crude drugs produced by one manufacturer accounted for 84%. Slightly more than half (54%) of drug recalls were due to a violation of the regulations. One manufacturer recalled many drugs because of non-compliance with the standard regulations for manufacturing drugs after 2011. In conclusion, non-prescription drug recalls can occur for any drug regardless of the dosage form and therapeutic category.
- Published
- 2016
29. Relationship between the plasma fentanyl and serum 4β-hydroxycholesterol based on CYP3A5 genotype and gender in patients with cancer pain
- Author
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Hikaru Sato, Junichi Kawakami, Takafumi Naito, and Takuya Ishida
- Subjects
Male ,medicine.medical_specialty ,Genotype ,Pharmaceutical Science ,Pharmacology ,030226 pharmacology & pharmacy ,Fentanyl ,03 medical and health sciences ,0302 clinical medicine ,Pharmacokinetics ,Internal medicine ,polycyclic compounds ,medicine ,Cytochrome P-450 CYP3A ,Humans ,Pharmacology (medical) ,CYP3A5 ,Aged ,Sex Characteristics ,business.industry ,Cancer ,Cancer Pain ,Middle Aged ,medicine.disease ,Hydroxycholesterols ,Analgesics, Opioid ,Endocrinology ,030220 oncology & carcinogenesis ,lipids (amino acids, peptides, and proteins) ,Female ,4β hydroxycholesterol ,Cancer pain ,business ,Sex characteristics ,medicine.drug - Abstract
This study aimed to evaluate the relationship between the concentrations of plasma fentanyl and serum 4β-hydroxycholesterol based on CYP3A5 genotype and gender in cancer patients. Thirty-three Japanese cancer patients treated with transdermal fentanyl were enrolled. The concentrations of plasma fentanyl and norfentanyl, and serum 4β-hydroxycholesterol and total cholesterol were determined at day 8 or later after starting the medication. The plasma fentanyl concentration was significantly higher in the CYP3A5*3/*3 group than in the *1 allele carrier group. The *3/*3 group had a lower metabolic ratio of fentanyl. The serum 4β-hydroxycholesterol concentration and its ratio to total cholesterol were significantly lower in the CYP3A5*3/*3 group than in the *1 allele carrier group. The concentrations of plasma fentanyl and serum 4β-hydroxycholesterol were significantly higher in women than in men. Gender did not affect the metabolic ratio of fentanyl or the concentration ratio of 4β-hydroxycholesterol. The plasma fentanyl concentration was not correlated with the serum 4β-hydroxycholesterol concentration, while the metabolic ratio of fentanyl was slightly correlated with the serum concentration ratio of 4β-hydroxycholesterol. In conclusion, CYP3A5*3 and gender affected the plasma fentanyl and serum 4β-hydroxycholesterol concentrations in cancer patients. However, the plasma disposition of fentanyl was not determined using the serum 4β-hydroxycholesterol concentration.
- Published
- 2016
30. Sarcomatoid carcinoma of the penis
- Author
-
Takuya Ishida, Teiichiro Aoyagi, and Isao Kuroda
- Subjects
medicine.medical_specialty ,Blood transfusion ,business.industry ,Cystostomy ,Inguinal lymph nodes ,medicine.medical_treatment ,General Medicine ,medicine.disease ,Surgery ,Metastasis ,medicine.anatomical_structure ,Quality of life ,Medicine ,business ,Penile mass ,Sarcomatoid carcinoma ,Penis - Abstract
We encountered a 63-year-old male with a sarcomatoid carcinoma of the penis accompanied by metastasis to the bilateral inguinal lymph nodes and lungs. He noticed a penile mass, but neglected it. The mass rapidly increased in size, forming an ulcer, and began to disintegrate. He visited our hospital 4 months after noticing the mass. After cystostomy and blood transfusion, surgical resection was performed. A diagnosis of a squamous cell carcinoma with a sarcomatoid carcinoma of the penis was made. After the operation, best supportive care (BSC) was selected. We made efforts to maintain his quality of life (QOL), and he died 3 months after the operation.
- Published
- 2014
31. Suprathreshold stochastic resonance in visual signal detection
- Author
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Sadatsugu Sakane, Ryozo Aoki, Tahei Kitamura, Takuya Ishida, Masayoshi Todorokihara, and Hitoshi Sasaki
- Subjects
Adult ,Male ,Signal Detection, Psychological ,Visual perception ,Adolescent ,genetic structures ,Stochastic resonance ,Models, Neurological ,behavioral disciplines and activities ,Signal ,Behavioral Neuroscience ,Nuclear magnetic resonance ,Sensory threshold ,Psychophysics ,Reaction Time ,medicine ,Humans ,Detection theory ,Physics ,Analysis of Variance ,Stochastic Processes ,Communication ,business.industry ,Noise (signal processing) ,Bruit ,Acoustic Stimulation ,Sensory Thresholds ,Auditory Perception ,Visual Perception ,Female ,sense organs ,medicine.symptom ,Noise ,business ,Photic Stimulation ,psychological phenomena and processes - Abstract
The present study aimed to demonstrate stochastic resonance (SR) for a visual signal above the threshold by using a two-alternative forced-choice protocol. The percent correct response enhanced at non-zero intensity of noise, while decreased with more intense noise. SR for the suprathreshold signals was found for signals with relatively low intensity above the threshold, and disappeared for more intense signals. These findings show the suprathreshold SR in the visual perception.
- Published
- 2008
32. Use of T1‐weighted/T2‐weighted magnetic resonance ratio images to elucidate changes in the schizophrenic brain
- Author
-
Tomohiro Donishi, Takuya Ishida, Kazuhiro Shinosaki, Yoshiki Kaneoke, Masaki Terada, Satoshi Ukai, and Jun Iwatani
- Subjects
Adult ,Male ,nucleus accumbens ,computer.software_genre ,White matter ,Behavioral Neuroscience ,Nuclear magnetic resonance ,Neurodevelopmental disorder ,Voxel ,Fractional anisotropy ,medicine ,Humans ,Gray Matter ,Myelin Sheath ,Original Research ,Cerebral Cortex ,Positive and Negative Syndrome Scale ,medicine.diagnostic_test ,ventral putamen ,Putamen ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,White Matter ,schizophrenia ,myelin ,T1‐weighted/T2‐weighted ratio ,medicine.anatomical_structure ,Cerebral cortex ,Case-Control Studies ,Female ,Psychology ,computer ,Neuroscience - Abstract
Introduction One leading hypothesis suggests that schizophrenia (SZ) is a neurodevelopmental disorder caused by genetic defects in association with environmental risk factors that affect synapse and myelin formation. Recent magnetic resonance imaging (MRI) studies of SZ brain showed both gray matter (GM) reduction and white matter (WM) fractional anisotropy reduction. In this study, we used T1-weighted (T1w)/T2-weighted (T2w) MRI ratio images, which increase myelin-related signal contrast and reduce receiver-coil bias. Methods We measured T1w/T2w ratio image signal intensity in 29 patients with SZ and 33 healthy controls (HCs), and then compared them against bias-corrected T1w images. Results Mean T1w/T2w ratio signal intensity values across all SZ GM and WM voxels were significantly lower than those for the HC values (analysis of covariance with age, gender, handedness, and premorbid intelligence quotient as nuisance covariates). SZ mean WM T1w/T2w ratio values were related to Global Assessment of Functioning (GAF) scores and were inversely related to the positive psychotic symptoms of the Positive and Negative Syndrome Scale. Voxel-based analysis revealed significantly lower T1w/T2w ratio image signal intensity values in the right ventral putamen in SZ GM. T1w image intensities did not differ between the SZ and HC groups. Conclusions T1-weighted/T2-weighted ratio imaging increased the detectability of SZ pathological changes. Reduced SZ brain signal intensity is likely due to diminished myelin content; therefore, mapping myelin-related SZ brain changes using T1w/T2w ratio images may be useful for studies of SZ brain abnormalities.
- Published
- 2015
33. Characterization of sulfur deposition over the period of industrialization in Japan using sulfur isotope ratio in Japanese cedar tree rings taken from stumps
- Author
-
Ichiro Tayasu, Takuya Ishida, and Chisato Takenaka
- Subjects
Biogeochemical cycle ,Time Factors ,Cryptomeria ,Air pollution ,chemistry.chemical_element ,Mineralogy ,Management, Monitoring, Policy and Law ,Forests ,medicine.disease_cause ,chemistry.chemical_compound ,Japan ,Sulfur Isotopes ,Dendrochronology ,medicine ,Sulfur Dioxide ,Industrial Development ,Sulfur dioxide ,General Environmental Science ,biology ,Geology ,General Medicine ,biology.organism_classification ,Pollution ,Sulfur ,Deposition (aerosol physics) ,chemistry ,Environmental chemistry ,Woody plant ,Environmental Monitoring - Abstract
We characterized the sulfur deposition history over the period of industrialization in Japan based on the sulfur isotope ratio (δ(34)S) in tree rings of Japanese cedar (Cryptomeria japonica D. Don) stumps. We analyzed and compared δ(34)S values in the rings from two types of disk samples from 170-year-old stumps that had been cut 5 years earlier (older forest stand) and from 40-year-old living trees (younger forest stand) in order to confirm the validity of using stump disks for δ(34)S analysis. No differences in δ(34)S values by age were found between the sample types, indicating that stump disks can be used for δ(34)S analysis. The δ(34)S profile in tree rings was significantly correlated with anthropogenic SO2 emissions in Japan (r = -0.76, p
- Published
- 2014
34. Interhemispheric disconnectivity in the sensorimotor network in bipolar disorder revealed by functional connectivity and diffusion tensor imaging analysis
- Author
-
Kazuhiro Shinosaki, Satoshi Ukai, Tomohiro Donishi, Yoshiki Kaneoke, Jun Iwatani, Shinichi Yamada, Shun Takahashi, Masaki Terada, and Takuya Ishida
- Subjects
Corpus callosum ,Somatosensory system ,Young Mania Rating Scale ,Article ,03 medical and health sciences ,0302 clinical medicine ,Fractional anisotropy ,medicine ,Bipolar disorder ,lcsh:Social sciences (General) ,lcsh:Science (General) ,Psychiatry ,Multidisciplinary ,medicine.diagnostic_test ,Confounding ,medicine.disease ,030227 psychiatry ,nervous system ,lcsh:H1-99 ,Medical imaging ,Functional magnetic resonance imaging ,Psychology ,Neuroscience ,030217 neurology & neurosurgery ,lcsh:Q1-390 ,Diffusion MRI - Abstract
Background Little is known regarding interhemispheric functional connectivity (FC) abnormalities via the corpus callosum in subjects with bipolar disorder (BD), which might be a key pathophysiological basis of emotional processing alterations in BD. Methods We performed tract-based spatial statistics (TBSS) using diffusion tensor imaging (DTI) in 24 healthy control (HC) and 22 BD subjects. Next, we analyzed the neural networks with independent component analysis (ICA) in 32HC and 25 BD subjects using resting-state functional magnetic resonance imaging. Results In TBSS analysis, we found reduced fractional anisotropy (FA) in the corpus callosum of BD subjects. In ICA, functional within-connectivity was reduced in two clusters in the sensorimotor network (SMN) (right and left primary somatosensory areas) of BD subjects compared with HCs. FC between the two clusters and FA values in the corpus callosum of BD subjects was significantly correlated. Further, the functional within-connectivity was related to Young Mania Rating Scale (YMRS) total scores in the right premotor area in the SMN of BD subjects. Limitations Almost all of our BD subjects were taking several medications which could be a confounding factor. Conclusions Our findings suggest that interhemispheric FC dysfunction in the SMN is associated with the impaired nerve fibers in the corpus callosum, which could be one of pathophysiological bases of emotion processing dysregulation in BD patients.
- Published
- 2017
35. Degree of Sulfate Saturation in Forest Soils Affected by Past Heavy Anthropogenic Deposition
- Author
-
Chisato Takenaka and Takuya Ishida
- Subjects
Environmental Engineering ,Chemistry ,Ecological Modeling ,Degree of saturation ,Air pollution ,chemistry.chemical_element ,medicine.disease_cause ,Pollution ,Sulfur ,chemistry.chemical_compound ,Adsorption ,Environmental chemistry ,Soil water ,medicine ,Environmental Chemistry ,Freundlich equation ,Sulfate ,Saturation (chemistry) ,Water Science and Technology - Abstract
The aims of this study were to assess sulfate adsorption in Japanese forest soils and to find and evaluate traces of past heavy anthropogenic sulfur deposition based on the degree of saturation as calculated based on the theoretical adsorption capacity determined by isotherm experiments and the amount of actual adsorbed sulfate. Investigations were conducted at two forest sites, a site in Yokkaichi that is exposed to serious air pollution containing sulfur compounds and a site in Inabu that is unpolluted. The distribution of phosphate-extractable sulfate concentration did not differ between the Yokkaichi site (1.11–13.2 mmol kg−1) and the Inabu site (0.40–11.0 mmol kg−1), and the values were higher than published data for North America and Europe. In contrast, the degree of sulfate saturation in soils of the Yokkaichi site was higher than that in soils of the Inabu site. These results indicate that the degree of sulfate saturation is valuable information for the evaluation of sulfur deposition history. The higher degree of saturation at Yokkaichi site may be due to enhanced sulfate adsorption by soils resulting from substantial past sulfur deposition.
- Published
- 2014
36. Evaluation of selective competitive binding of basic drugs to alpha1-acid glycoprotein variants
- Author
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Kunizo Arai, Yukio Suga, Junko Ishizaki, Ken-ichi Miyamoto, Akiko Fukaishi, Koichi Yokogawa, Satoko Yamazaki, Takuya Ishida, Mayu Tabata, and Chie Fukuwa
- Subjects
Inhibition constant ,Circular dichroism ,Dicumarol ,Stereochemistry ,Drug interaction ,Pharmaceutical Science ,Dissociation constant ,Binding, Competitive ,chemistry.chemical_compound ,Competitive binding ,medicine ,Protein binding ,Humans ,Drug Interactions ,Variant ,Pharmacology ,chemistry.chemical_classification ,α1-acid glycoprotein ,Circular Dichroism ,Spectrum Analysis ,Acridine orange ,Genetic Variation ,General Medicine ,Dipyridamole ,Orosomucoid ,Acridine Orange ,Biochemistry ,chemistry ,Pharmaceutical Preparations ,α1 acid glycoprotein ,Selectivity ,Glycoprotein ,Disopyramide ,medicine.drug - Abstract
金沢大学医薬保健研究域薬学系, We examined the binding of various basic drugs to the F1S and A genetic variants of α1-acid glycoprotein (AGP), which were isolated from native human commercial AGP (total AGP) by chromatography on an immobilized copper(II) affinity adsorbent. The values of the dissociation constant (Kd) of some basic drugs with the F1S variant in equilibrium dialysis differed characteristically from those with the A variant. The selective binding to these variants was evaluated by measuring the displacement ratio of dicumarol bound to the F1S variant or that of acridine orange bound to the A variant, using circular dichroism spectroscopy. There was reasonably good agreement between the Kd values and displacement ratios. There was a characteristic difference between the values of inhibition constant (Ki) of basic drugs towards dipyridamole binding to F1S and towards disopyramide binding to A in total AGP. We found that the Ki values for dipyridamole binding were well correlated with the Kd values for the F1S variant, whereas those for disopyramide binding were well correlated with the Kd values for the A variant. In conclusion, the higher the affinity of basic drugs for AGP, the more they inhibit the binding of other basic drugs, and further, the inhibitory potency depends on the selectivity of binding to the AGP variants. © 2010 Pharmaceutical Society of Japan.
- Published
- 2010
37. Relationship Between Amlodipine Pharmacokinetics And Cyp3a Activity In Lactating Women With Pregnancy-Induced Hypertension
- Author
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M. Sugihara, Hiroaki Itoh, Takafumi Naito, S. Deguchi, Junichi Kawakami, Naohiro Kanayama, Takuya Ishida, and Naoko Kubono
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Pharmacology ,Pharmacokinetics ,CYP3A ,business.industry ,medicine ,Pregnancy induced ,Pharmacology (medical) ,Amlodipine ,business ,medicine.drug - Published
- 2015
38. Relationships Between Plasma Concentrations of Fentanyl and 4beta-Hydroxycholesterol In Cancer Patients
- Author
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Hikaru Sato, Junichi Kawakami, Kazunori Ohnishi, Takuya Ishida, and Takafumi Naito
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Pharmacology ,business.industry ,Anesthesia ,Plasma concentration ,Medicine ,Cancer ,Pharmacology (medical) ,business ,medicine.disease ,Fentanyl ,medicine.drug - Published
- 2015
39. Real-Time Dynamic Adsorption Processes of Cytochrome c on an Electrode Observed through Electrochemical High-Speed Atomic Force Microscopy
- Author
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Hiroyuki Ohno, Takayuki Uchihashi, Kouta Takeda, Takuya Ishida, Nobuhumi Nakamura, Hiroki Watanabe, and Kiyohiko Igarashi
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Models, Molecular ,medicine.medical_specialty ,Time Factors ,Materials science ,Protein Conformation ,lcsh:Medicine ,Microscopy, Atomic Force ,Electrochemistry ,Redox ,Electron transfer ,Adsorption ,medicine ,Animals ,Sulfhydryl Compounds ,lcsh:Science ,Electrodes ,Bioelectronics ,Multidisciplinary ,lcsh:R ,Fatty Acids ,Cytochromes c ,Chemical engineering ,Standard electrode potential ,Bioelectrochemistry ,Electrode ,lcsh:Q ,Gold ,Research Article - Abstract
An understanding of dynamic processes of proteins on the electrode surface could enhance the efficiency of bioelectronics development and therefore it is crucial to gain information regarding both physical adsorption of proteins onto the electrode and its electrochemical property in real-time. We combined high-speed atomic force microscopy (HS-AFM) with electrochemical device for simultaneous observation of the surface topography and electron transfer of redox proteins on an electrode. Direct electron transfer of cytochrome c (cyt c) adsorbed on a self-assembled monolayers (SAMs) formed electrode is very attractive subject in bioelectrochemistry. This paper reports a real-time visualization of cyt c adsorption processes on an 11-mercaptoundecanoic acid-modified Au electrode together with simultaneous electrochemical measurements. Adsorbing cyt c molecules were observed on a subsecond time resolution simultaneously with increasing redox currents from cyt c using EC-HS-AFM. The root mean square roughness (R RMS) from the AFM images and the number of the electrochemically active cyt c molecules adsorbed onto the electrode (Γ) simultaneously increased in positive cooperativity. Cyt c molecules were fully adsorbed on the electrode in the AFM images when the peak currents were steady. This use of electrochemical HS-AFM significantly facilitates understanding of dynamic behavior of biomolecules on the electrode interface and contributes to the further development of bioelectronics.
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- 2015
40. PP202—Cancer Cachexia Raises the Plasma Concentration of Oxymorphone Through the Reduction of CYP3A but not CYP2D6 in Oxycodone-Treated Patients
- Author
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Junichi Kawakami, Takuya Ishida, Kazunori Ohnishi, Takafumi Naito, and M. Tashiro
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Pharmacology ,CYP2D6 ,CYP3A ,business.industry ,medicine.medical_treatment ,Cancer cachexia ,Oxymorphone ,Anesthesia ,Plasma concentration ,medicine ,Pharmacology (medical) ,business ,Oxycodone ,Reduction (orthopedic surgery) ,medicine.drug - Published
- 2013
- Full Text
- View/download PDF
41. Downregulation of oxytocin expression in the supraoptic nucleus of traumatic stress-treated rat
- Author
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Mitsuhiro Kawata, Isao Nishimura, Kenji Fukui, Takashi Hashimoto, Takuya Ishida, Takanobu Yoshii, Ken-ichi Matsuda, and Hideto Tsuchida
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medicine.medical_specialty ,Chemistry ,General Neuroscience ,Traumatic stress ,Neuropeptide ,General Medicine ,Oxytocin receptor ,Supraoptic nucleus ,Endocrinology ,Oxytocin ,Downregulation and upregulation ,Internal medicine ,medicine ,medicine.drug - Published
- 2011
42. Reduced dorsolateral prefrontal cortical hemodynamic response in adult obsessive-compulsive disorder as measured by near-infrared spectroscopy during the verbal fluency task
- Author
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Seiji Nishida, Takuya Ishida, Jin Narumoto, Yuichi Takei, Yuki Sakai, Rikuei Hirosawa, Takashi Nakamae, and Kenji Fukui
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medicine.medical_specialty ,Neuropsychiatric Disease and Treatment ,near-infrared spectroscopy ,functional neuroimaging ,Dorsolateral ,Audiology ,behavioral disciplines and activities ,Functional neuroimaging ,Medicine ,Verbal fluency test ,Psychiatry ,verbal fluency task ,Biological Psychiatry ,Original Research ,dorsolateral prefrontal cortex ,medicine.diagnostic_test ,business.industry ,Cognition ,obsessive-compulsive disorder ,Dorsolateral prefrontal cortex ,Psychiatry and Mental health ,medicine.anatomical_structure ,prefrontal hemodynamic response ,Anxiety ,Orbitofrontal cortex ,medicine.symptom ,business ,Functional magnetic resonance imaging - Abstract
Rikuei Hirosawa,1 Jin Narumoto,1 Yuki Sakai,1 Seiji Nishida,2 Takuya Ishida,1 Takashi Nakamae,1 Yuichi Takei,3 Kenji Fukui1 1Department of Psychiatry, Graduate School of Medicine, Kyoto Prefectural University of Medicine, Kyoto, 2Maizuru Medical Center, Kyoto, 3Department of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Gunma, Japan Background: Near-infrared spectroscopy has helped our understanding of the neurobiological mechanisms of psychiatric disorders and has advantages including noninvasiveness, lower cost, and ease of use compared with other imaging techniques, like functional magnetic resonance imaging. The verbal fluency task is the most common and well established task used to assess cognitive activation during near-infrared spectroscopy. Recent functional neuroimaging studies have shown that the orbitofrontal cortex and other brain regions, including the dorsolateral prefrontal cortex, may play important roles in the pathophysiology of obsessive-compulsive disorder (OCD). This study aimed to evaluate hemodynamic responses in the dorsolateral prefrontal cortex in patients with OCD using near-infrared spectroscopy during the verbal fluency task and to compare these with dorsolateral prefrontal cortex responses in healthy controls. Methods: Twenty patients with OCD and 20 controls matched for age, gender, handedness, and estimated intelligence quotient participated in this study. The verbal fluency task was used to elicit near-infrared spectroscopic activation and consisted of a 30-second pre-task, followed by three repetitions of a 20-second verbal fluency task (total 60 seconds), followed by a 70-second post-task period. The near-infrared spectroscopy experiment was conducted on the same day as surveys of obsessive-compulsive symptoms, depression, and anxiety. Z-scores for changes in the concentration of oxygenated hemoglobin were compared between the OCD patients and controls in 14 channels set over the left and right dorsolateral prefrontal cortex and frontopolar areas. Results: During the verbal fluency task, significant task-related activation was detected in both the OCD group and the controls. Changes in oxygenated hemoglobin concentration in the right dorsolateral prefrontal cortex were significantly smaller in the OCD group than in the controls, but were not statistically significant after correction for multiple comparisons. Conclusion: Patients with OCD have reduced prefrontal, especially right dorsolateral prefrontal, cortical hemodynamic responses as measured by near-infrared spectroscopy during the verbal fluency task. These results support the hypothesis that the dorsolateral prefrontal cortex plays a role in the pathophysiology of OCD. Keywords: functional neuroimaging, near-infrared spectroscopy, obsessive-compulsive disorder, prefrontal hemodynamic response, verbal fluency task, dorsolateral prefrontal cortex
- Published
- 2013
43. Results of 271 funnel chest operations
- Author
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Tadashi Hasegawa, Takuya Ishida, Koji Ikeda, and Juro Wada
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Pulmonary and Respiratory Medicine ,Adult ,Postoperative Care ,medicine.medical_specialty ,Sternum ,Funnel Chest ,Adolescent ,business.industry ,MEDLINE ,Infant ,Ribs ,Child, Preschool ,Methods ,Medicine ,Humans ,Surgery ,Radiology ,Thoracoplasty ,Cardiology and Cardiovascular Medicine ,business ,Child - Published
- 1970
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