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3 results on '"Tao-xing Shi"'

1. Decision support system for the response to infectious disease emergencies based on WebGIS and mobile services in China.

Author

Ya-pin Li, Li-qun Fang, Su-qing Gao, Zhen Wang, Hong-wei Gao, Peng Liu, Ze-Rui Wang, Yan-Li Li, Xu-Guang Zhu, Xin-Lou Li, Bo Xu, Yin-Jun Li, Hong Yang, Sake J de Vlas, Tao-Xing Shi, and Wu-Chun Cao

Subjects
Medicine, Science
Abstract

BackgroundFor years, emerging infectious diseases have appeared worldwide and threatened the health of people. The emergence and spread of an infectious-disease outbreak are usually unforeseen, and have the features of suddenness and uncertainty. Timely understanding of basic information in the field, and the collection and analysis of epidemiological information, is helpful in making rapid decisions and responding to an infectious-disease emergency. Therefore, it is necessary to have an unobstructed channel and convenient tool for the collection and analysis of epidemiologic information in the field.Methodology/principal findingsBaseline information for each county in mainland China was collected and a database was established by geo-coding information on a digital map of county boundaries throughout the country. Google Maps was used to display geographic information and to conduct calculations related to maps, and the 3G wireless network was used to transmit information collected in the field to the server. This study established a decision support system for the response to infectious-disease emergencies based on WebGIS and mobile services (DSSRIDE). The DSSRIDE provides functions including data collection, communication and analyses in real time, epidemiological detection, the provision of customized epidemiological questionnaires and guides for handling infectious disease emergencies, and the querying of professional knowledge in the field. These functions of the DSSRIDE could be helpful for epidemiological investigations in the field and the handling of infectious-disease emergencies.Conclusions/significanceThe DSSRIDE provides a geographic information platform based on the Google Maps application programming interface to display information of infectious disease emergencies, and transfers information between workers in the field and decision makers through wireless transmission based on personal computers, mobile phones and personal digital assistants. After a 2-year practice and application in infectious disease emergencies, the DSSRIDE is becoming a useful platform and is a useful tool for investigations in the field carried out by response sections and individuals. The system is suitable for use in developing countries and low-income districts.

Published
2013
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2. A dataset of distribution and diversity of blood-sucking mites in China

Author

Fan-Fei Meng, Zheng-Wei Fan, Guo-Ping Zhao, Qiang Xu, Wei Liu, Yang Ji, Tao-Xing Shi, Wen-Hui Zhang, Liqun Fang, Jin-Jin Chen, and Bao-Gui Jiang

Subjects
Statistics and Probability, Entomology, China, Data Descriptor, Epidemiology, media_common.quotation_subject, Science, Distribution (economics), Scrub typhus, Library and Information Sciences, Education, immune system diseases, parasitic diseases, medicine, Mite, Animals, media_common, Mites, biology, Geography, integumentary system, Ecology, business.industry, Species diversity, medicine.disease, biology.organism_classification, respiratory tract diseases, Computer Science Applications, Arachnid Vectors, Statistics, Probability and Uncertainty, Risk assessment, business, Animal Distribution, Diversity (politics), Information Systems
Abstract

Mite-borne diseases, such as scrub typhus and hemorrhagic fever with renal syndrome, present an increasing global public health concern. Most of the mite-borne diseases are caused by the blood-sucking mites. To present a comprehensive understanding of the distributions and diversity of blood-sucking mites in China, we derived information from peer-reviewed journal articles, thesis publications and books related to mites in both Chinese and English between 1978 and 2020. Geographic information of blood-sucking mites’ occurrence and mite species were extracted and georeferenced at the county level. Standard operating procedures were applied to remove duplicates and ensure accuracy of the data. This dataset contains 6,443 records of mite species occurrences at the county level in China. This geographical dataset provides an overview of the species diversity and wide distributions of blood-sucking mites, and can potentially be used in distribution prediction of mite species and risk assessment of mite-borne diseases in China., Measurement(s) Distribution Technology Type(s) digital curation • Geographic Information System Factor Type(s) genus • species • province • city • county • GPS Sample Characteristic - Organism mite • Acari Sample Characteristic - Location China Machine-accessible metadata file describing the reported data: 10.6084/m9.figshare.14459055

Published
2021

3. Different strategies for preparation of non-tagged rV270 protein and its efficacy against Yersinia pestis challenge

Author

Yonghai Yang, Baizhong Cui, Wang Wang, Tao-Xing Shi, Hu Wang, Zhizhen Qi, Xiaoyi Wang, Ziwen Zhu, Ruifu Yang, Benchuan Wu, Qingwen Zhang, Zuyun Wang, Zhaobiao Guo, Yefeng Qiu, and Ruixia Dai

Subjects
Enteropeptidase, Pore Forming Cytotoxic Proteins, Yersinia pestis, Health, Toxicology and Mutagenesis, Protein subunit, Recombinant Fusion Proteins, Blotting, Western, Genetic Vectors, Molecular Sequence Data, Protein Engineering, law.invention, Fusion gene, Mice, Thrombin, Affinity chromatography, law, medicine, Escherichia coli, Animals, LcrV, Amino Acid Sequence, Cloning, Molecular, Antigens, Bacterial, Mice, Inbred BALB C, Plague, Plague Vaccine, biology, Chemistry, Public Health, Environmental and Occupational Health, biology.organism_classification, Virology, Molecular biology, Antibodies, Bacterial, Survival Analysis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vaccines, Subunit, Recombinant DNA, Electrophoresis, Polyacrylamide Gel, Female, medicine.drug, Plasmids
Abstract

Objective LcrV is an important component for the development of a subunit vaccine against plague. To reduce immunosuppressive activity of LcrV, a recombinant LcrV variant lacking amino acids 271 to 326 (rV270) was prepared by different methods in this study. Methods A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a, or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector pET32a. After Co2+ affinity chromatography, a purification strategy was developed by cleavage of His tag on column, following Sephacryl S-200HR column filtration chromatography. Results Removal of His tag by thrombin, enterokinase and factor Xa displayed a yield of 99.5%, 32.4% and 15.3%, respectively. Following Sephacryl S-200HR column filtration chromatography, above 97% purity of rV270 protein was obtained. Purified rV270 that was adsorbed to 25% (v/v) Al(OH)3 adjuvant in phosphate-buffered saline (PBS) induced very high titers of antibody to rV270 in BALB/c mice and protected them (100% survival) against subcutaneous challenge with 106 CFU of Y. pestis virulent strain 141. Conclusion The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa, but they exhibited extremely low cleavage activity to the corresponding recognition site. Thrombin cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage efficacy. The recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.

Published
2010

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