67 results on '"Xia, Kang"'
Search Results
2. FAP senescence: a critical event in muscle regeneration
- Author
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Xia Kang, Yuqi Gao, So-ichiro Fukada, and Hongming Miao
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Medicine ,Biology (General) ,QH301-705.5 - Published
- 2023
- Full Text
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3. Development of a valid Chinese version of the Cumberland Ankle Instability Tool in Chinese-speaking patients with chronic ankle instability disorders
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Wei Wang, Dongfa Liao, Xia Kang, Wei Zheng, Wei Xu, Song Chen, and Qingyun Xie
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Medicine ,Science - Abstract
Abstract As an effective scale for the condition assessment of patients with chronic ankle instability (CAI), the Cumberland Ankle Instability Tool (CAIT) is the most widely used scale, and its original version is written in English. Therefore, the purpose of our study is to apply the CAIT to Chinese patients and evaluate its responsiveness, reliability, and validity in terms of Chinese patients with CAI. First, we adapted the CAIT into the Chinese edition (CAIT-C), through which cross-cultural adaptation and translation can be carried out in a five-step procedure. Next, recruited patients completed the three periods of the Foot and Ankle Ability Measure (FAAM), CAIT-C, and the Medical Outcomes Study Short-Form 36 (SF-36) scales. Afterward, to assess the responsiveness, reliability, and validity, we calculated the standardized response mean (SRM), effect size (ES), Spearman's correlation coefficient (r s), minimal detectable change (MDC), standard error of measurement (SEM), intraclass correlation coefficient (ICC), and Cronbach’s alpha. Generally, in the use of CAI, 131, 119, and 86 patients favorably completed the three periods of the scales. The CAIT-C was proven to have good test–retest reliability (ICC = 0.930) and fine internal consistency (Cronbach’s alpha = 0.845–0.878). The low-value of MDC (0.04–2.28) and SEM (1.73) show it is possible to detect clinical changes when we take advantage of CAIT-C. Good or moderate correlations (r s = 0.422–0.738) were gained from the physical subscales of the SF-36 and the subscales of the FAAM and the CAIT-C. Fair or poor correlations (r s = 0.003–0.360) were gained between the mental subscales of the SF-36 and the CAIT-C, which sufficiently indicated that the CAIT-C had good validity. Moreover, good responsiveness was observed in the CAIT-C (ES = 1.316, SRM = 1.418). The CAIT-C scale is an effective, valid, and reliable tool to evaluate Chinese CAI patients.
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- 2021
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4. Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
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Jin Qian, Zi-chen Gong, Yi-na Zhang, Hong-hua Wu, Jing Zhao, Li-ting Wang, Li-juan Ye, Da Liu, Wei Wang, Xia Kang, Jun Sheng, Wei Xu, Xi-lin Liu, Juan Wu, and Wei Zheng
- Subjects
Lactic acid ,Bone metastasis ,Osteoclast precursors ,CXCL10 ,Cadherin-11 ,PI3K-AKT pathway ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background To investigate the effect of lactic acid (LA) on the progression of bone metastasis from colorectal cancer (CRC) and its regulatory effects on primary CD115 (+) osteoclast (OC) precursors. Methods The BrdU assay, Annexin-V/PI assay, TRAP staining and immunofluorescence were performed to explore the effect of LA on the proliferation, apoptosis and differentiation of OC precursors in vitro and in vivo. Flow cytometry was performed to sort primary osteoclast precursors and CD4(+) T cells and to analyze the change in the expression of target proteins in osteoclast precursors. A recruitment assay was used to test how LA and Cadhein-11 regulate the recruitment of OC precursors. RT-PCR and Western blotting were performed to analyze the changes in the mRNA and protein expression of genes related to the PI3K-AKT pathway and profibrotic genes. Safranin O-fast green staining, H&E staining and TRAP staining were performed to analyze the severity of bone resorption and accumulation of osteoclasts. Results LA promoted the expression of CXCL10 and Cadherin-11 in CD115(+) precursors through the PI3K-AKT pathway. We found that CXCL10 and Cadherin-11 were regulated by the activation of CREB and mTOR, respectively. LA-induced overexpression of CXCL10 in CD115(+) precursors indirectly promoted the differentiation of osteoclast precursors through the recruitment of CD4(+) T cells, and the crosstalk between these two cells promoted bone resorption in bone metastasis from CRC. On the other hand, Cadherin-11 mediated the adhesion between osteoclast precursors and upregulated the production of specific collagens, especially Collagen 5, which facilitated fibrotic changes in the tumor microenvironment. Blockade of the PI3K-AKT pathway efficiently prevented the progression of bone metastasis caused by lactate. Conclusion LA promoted metastatic niche formation in the tumor microenvironment through the PI3K-AKT pathway. Our study provides new insight into the role of LA in the progression of bone metastasis from CRC. Video Abstract
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- 2021
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5. Academic enjoyment, behavioral engagement, self-concept, organizational strategy and achievement in EFL setting: A multiple mediation analysis.
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Xia Kang and Yajun Wu
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Medicine ,Science - Abstract
Motivated by the positive psychology movement in the English as Foreign Language (EFL), existing studies have demonstrated that subject-related enjoyment has a positive correlation with academic achievement. However, quite a few studieshave examined why academic enjoyment can predict positive academic achievement. This study aimed to investigate whether behavioral engagement, self-concept, and organizational strategy mediated relations between academic enjoyment and achievement in EFL setting. 528 Chinese secondary school students (Male: N = 280; Female: N = 248) participated in the survey and completed the questionnaires on EFL-related enjoyment, behavioral engagement, self-concept, organizational strategy, and academic performance. Structural equation model (SEM) analyses showed that students' academic enjoyment positively predicted their English achievement. Academic engagement, self-concept, and organizational strategy had parallel multiple mediating effects between academic enjoyment and English achievement. Multi-group SEM analysis demonstrated that the model had invariance across genders, indicating that the model is applicable to both male and female students. Limitations and implications are discussed.
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- 2022
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6. Interleukin-15 facilitates muscle regeneration through modulation of fibro/adipogenic progenitors
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Xia Kang, Ming-yu Yang, You-xing Shi, Mei-ming Xie, Min Zhu, Xiao-long Zheng, Chen-ke Zhang, Zi-lu Ge, Xu-ting Bian, Jing-tong Lv, Yun-jiao Wang, Bing-hua Zhou, and Kang-lai Tang
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IL-15 ,Fibro/adipogenic progenitor ,Fatty infiltration ,Fibrosis ,Muscle injury ,Rotator cuff tear ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background Chronic muscle injury is characteristics of fatty infiltration and fibrosis. Recently, fibro/adipogenic progenitors (FAPs) were found to be indispensable for muscular regeneration while were also responsible for fibrosis and fatty infiltration in muscle injury. Many myokines have been proven to regulate the adipose or cell proliferation. Because the fate of FAPs is largely dependent on microenvironment and the regulation of myokines on FAPs is still unclear. We screened the potential myokines and found Interleukin-15 (IL-15) may regulate the fatty infiltration in muscle injury. In this study, we investigated how IL-15 regulated FAPs in muscle injury and the effect on muscle regeneration. Methods Cell proliferation assay, western blots, qRT-PCR, immunohistochemistry, flow cytometric analysis were performed to investigate the effect of IL-15 on proliferation and adipogensis of FAPs. Acute muscle injury was induced by injection of glycerol or cardiotoxin to analyze how IL-15 effected on FAPs in vivo and its function on fatty infiltration or muscle regeneration. Results We identified that the expression of IL-15 in injured muscle was negatively associated with fatty infiltration. IL-15 can stimulate the proliferation of FAPs and prevent the adipogenesis of FAPs in vitro and in vivo. The growth of FAPs caused by IL-15 was mediated through JAK-STAT pathway. In addition, desert hedgehog pathway may participate in IL-15 inhibiting adipogenesis of FAPs. Our study showed IL-15 can cause the fibrosis after muscle damage and promote the myofiber regeneration. Finally, the expression of IL-15 was positively associated with severity of fibrosis and number of FAPs in patients with chronic rotator cuff tear. Conclusions These findings supported the potential role of IL-15 as a modulator on fate of FAPs in injured muscle and as a novel therapy for chronic muscle injury.
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- 2018
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7. Author Correction: Development of a valid Chinese version of the Cumberland Ankle Instability Tool in Chinese-speaking patients with chronic ankle instability disorders
- Author
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Wei Wang, Dongfa Liao, Xia Kang, Wei Zheng, Wei Xu, Song Chen, and Qingyun Xie
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Medicine ,Science - Published
- 2021
- Full Text
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8. NF-κB potentiates tumor growth by suppressing a novel target LPTS
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Dongbo Liu, Hongping Miao, Yuanyin Zhao, Xia Kang, Shenglan Shang, Wei Xiang, Rongchen Shi, Along Hou, Rui Wang, Kun Zhao, Yingzhe Liu, Yue Ma, Huan Luo, Hongming Miao, and Fengtian He
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NF-κB ,LPTS ,Promoter ,Cervical cancer ,Colon cancer ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background Chronic inflammation is causally linked to the carcinogenesis and progression of most solid tumors. LPTS is a well-identified tumor suppressor by inhibiting telomerase activity and cancer cell growth. However, whether and how LPTS is regulated by inflammation signaling is still incompletely elucidated. Methods Real-time PCR and western blotting were used to determine the expression of p65 and LPTS. Reporter gene assay, electrophoretic mobility shift assay and chromatin immunoprecipitation were performed to decipher the regulatory mechanism between p65 and LPTS. Cell counting kit-8 assays and xenograt models were used to detect p65-LPTS-regulated cancer cell growth in vitro and in vivo, respectively. Results Here we for the first time demonstrated that NF-κB could inhibit LPTS expression in the mRNA and protein levels in multiple cancer cells (e.g. cervical cancer and colon cancer cells). Mechanistically, NF-κB p65 could bind to two consensus response elements locating at −1143/−1136 and −888/−881 in the promoter region of human LPTS gene according to EMSA and ChIP assays. Mutation of those two binding sites rescued p65-suppressed LPTS promoter activity. Functionally, NF-κB regulated LPTS-dependent cell growth of cervical and colon cancers in vitro and in xenograft models. In translation studies, we verified that increased p65 expression was associated with decreased LPTS level in multiple solid cancers. Conclusions Taken together, we revealed that NF-κB p65 potentiated tumor growth via suppressing a novel target LPTS. Modulation of NF-κB-LPTS axis represented a potential strategy for treatment of those inflammation-associated malignancies.
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- 2017
- Full Text
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9. Investigating the Linkage Between School Psychological Capital and Achievement Emotions in Secondary School Mathematics
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Xia Kang and Yajun Wu
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Pride ,media_common.quotation_subject ,education ,Shame ,Boredom ,Academic achievement ,Anger ,Structural equation modeling ,Education ,Developmental psychology ,medicine ,Anxiety ,medicine.symptom ,Sociology of Education ,media_common - Abstract
Research on school Psychological Capital (PsyCap) and achievement emotions have been carried out in parallel with almost no intersection. This study aims to explore the potential synergies between these two paradigms by investigating whether school PsyCap has a predictive effect on discrete achievement emotions. Chinese secondary school students (N = 1067) took part in the study. Structural Equation Modeling analyses were conducted to examine the linkage between these two variables after controlling for demographic variables of gender and year level, and crucial antecedents of parental expectations, the teacher-student relationship, and prior academic achievement. Results indicated that individual variation in school PsyCap was the significant predictor of achievement emotions. Specifically, school PsyCap was positively correlated with positive achievement emotions such as enjoyment, hope, and pride, while it was negatively correlated with negative achievement emotions such as anger, anxiety, shame, boredom, and hopelessness. Both limitations and implications are discussed.
- Published
- 2021
10. Exosome‐transmitted circ_MMP2 promotes hepatocellular carcinoma metastasis by upregulating MMP2
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Ming-Tai Gao, Fang Zhang, Dongqin Chen, Mianli Li, Li Jin, Linghui Xiao, Deng-Rui Liu, and Hong-Xia Kang
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Male ,0301 basic medicine ,Cancer Research ,MMP2 ,Exosomes ,medicine.disease_cause ,Metastasis ,0302 clinical medicine ,Neoplasm Metastasis ,circ_MMP2 ,HCC ,Research Articles ,Liver Neoplasms ,General Medicine ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Liver ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Matrix Metalloproteinase 2 ,Molecular Medicine ,Research Article ,Carcinoma, Hepatocellular ,Mice, Nude ,Biology ,Models, Biological ,Exosome ,lcsh:RC254-282 ,03 medical and health sciences ,Downregulation and upregulation ,Cell Line, Tumor ,Genetics ,medicine ,Animals ,Humans ,exosome ,metastasis ,Gene Silencing ,Base Sequence ,RNA, Circular ,medicine.disease ,Microvesicles ,digestive system diseases ,MicroRNAs ,030104 developmental biology ,Tumor progression ,Cancer research ,Carcinogenesis - Abstract
Exosomes released by tumor cells have been recently identified as important determinants of tumor progression. They often carry circular RNAs that are differentially expressed in tumors and may regulate tumorigenesis and metastasis. Here, we showed that supernatant of 97H hepatocellular carcinoma (HCC) cell line could promote metastasis in L02 human liver cells and HCC cell lines. Moreover, we determined that circ_MMP2 (has_circ_0039411) could be delivered by 97H‐ or LM3 cell‐derived exosomes to L02 and HepG2 cell cultures. High expression of circ_MMP2 led to the upregulation of its host gene matrix metallopeptidase 2 (MMP2) via the sponging of miR‐136‐5p. Rescue assays demonstrated that miR‐136‐5p and MMP2 were two essential participants in HCC metastasis. Finally, high level of circ_MMP2 or MMP2, as well as low level of miR‐136‐5p, was correlated with low overall survival of HCC patients. Our study highlights a novel molecular pathway in HCC cell‐derived exosomes., 97H and LM3 cell line cocultured together had strong metastasis capacity. They released exosomes to produce circ_MMP2 to sponge miR‐136‐5p by enhancing MMP2 to accelerate the process hepatocellular cancinoma.
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- 2020
11. A microfluidics-derived growth factor gradient in a scaffold regulates stem cell activities for tendon-to-bone interface healing
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Zhiyu He, Long Chen, Jingtong Lyu, Yunjiao Wang, Wenjie Wu, Xia Kang, Kanglai Tang, Jiqiang Zhang, Jun Wu, and Hong Tang
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Male ,Scaffold ,Biomedical Engineering ,Biocompatible Materials ,02 engineering and technology ,Bone and Bones ,Rats, Sprague-Dawley ,Tendons ,Extracellular matrix ,03 medical and health sciences ,Tissue engineering ,Lab-On-A-Chip Devices ,medicine ,Animals ,General Materials Science ,030304 developmental biology ,Wound Healing ,0303 health sciences ,Decellularization ,Tissue Scaffolds ,Chemistry ,Stem Cells ,Biomaterial ,021001 nanoscience & nanotechnology ,Rats ,Tendon ,medicine.anatomical_structure ,Intercellular Signaling Peptides and Proteins ,Stem cell ,0210 nano-technology ,Wound healing ,Biomedical engineering - Abstract
Treatment of tendon-to-bone interface injury has long been challenging in sports medicine. The major obstacle lies with the complicated three-layer structure of the tissue that consists of a bone region with osteocytes, a tendon region with tenocytes and a transitional region with chondrocytes. Conventional tissue engineering approaches using simply biomaterial scaffolds, stem cells and combinations of them had limited abilities to reconstruct the gradient structure with normal biomechanical properties. We herein aim to construct a three-layer structure with bone marrow-derived stem cells and tendon stem cells cultured in a decellularized tendon scaffold, through application of a gradient of biological cues in the longitudinal direction of the scaffold that guides the stem cells to differentiate and remodel the extracellular matrix in response to different medium concentrations in different regions. A microfluidic chip, on which a tree-like flow pattern was implemented, was adopted to create the concentration gradient in a dichotomous manner. We screened for an optimized seeding ratio between the two stem cell types before incubation of the scaffold in the medium concentration gradient and surgical implantation. Histology and immunohistochemistry assessments, both qualitatively and semi-quantitatively, showed that the microfluidic system provided desired guidance to the seeded stem cells that the healing at 8-week post-implantation presented a similar structure to that of a normal tendon-to-bone interface, which was outstanding compared to treatments without gradient guidance, stem cells or scaffolds where chaotic and fibrotic structures were obtained. This strategy offers a potentially translational tissue engineering approach for better outcomes in tendon-to-bone healing.
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- 2020
12. A family with nemaline myopathy type 6 caused by hseterozygous mutation (c.1222C>T) in the KBTBD13 gene in China: A case report
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Jing Miao, Xiao-jing Wei, Zhi-xia Kang, Yan-lu Gao, Xue-Fan Yu, and Zi-Yi Wang
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Proximal muscle weakness ,Muscle biopsy ,medicine.diagnostic_test ,Muscle weakness ,General Medicine ,Biology ,medicine.disease ,Congenital myopathy ,Molecular biology ,Muscle atrophy ,Hypotonia ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Nemaline myopathy ,030220 oncology & carcinogenesis ,Mutation (genetic algorithm) ,medicine ,heterocyclic compounds ,Neurology (clinical) ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
Nemaline myopathy (NEM) is a congenital myopathy that typically presents with proximal muscle weakness and hypotonia. To date, 13 genes have been associated with NEM. The Kelch repeat and BTB domain-containing protein 13 (KBTBD13) gene (KBTBD13)-related NEM is a rarely reported condition, and not a single case has been reported in Asia. Here, we report the case of a mother and daughter in China with NEM caused by a mutation (c.1222C>T) in KBTBD13. Their shared clinical phenotype is symmetrical muscle weakness in the arms and legs with childhood onset. Muscle magnetic resonance imaging showed the unique replacement mode of muscle with fibro-fatty tissue. Histopathological examination revealed the presence of fibers containing rod-shaped structures in the cytoplasm or under the sarcolemma. DNA sequencing analysis detected a heterozygous mutation (c.1222C>T) in KBTBD13 in this family. A founder effect for the variant may exist in the Low Countries of Belgium and the Netherlands, and the mutation may be a hotspot mutation in Europe, as it has not been reported in Asia. Our case study expands the spectrum of KBTBD13-related NEM.
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- 2019
13. Macrophage ABHD5 Suppresses NFκB-Dependent Matrix Metalloproteinase Expression and Cancer Metastasis
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Xinran Ji, Xia Kang, Peng Chen, Rongchen Shi, Hongming Miao, Dapeng Zhang, Lili Zhang, Jun Chen, Rongyang Dai, Fan Yang, Shenglan Shang, Wei Xiang, Yongsheng Li, Chuan Li, Shan Chen, and Yongchuan Chen
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Male ,0301 basic medicine ,Cancer Research ,Colorectal cancer ,Matrix metalloproteinase ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,Tumor Microenvironment ,medicine ,Animals ,Humans ,Macrophage ,Neoplasm Metastasis ,Regulation of gene expression ,Activator (genetics) ,Chemistry ,Macrophages ,NF-kappa B ,Transcription Factor RelA ,Cancer ,1-Acylglycerol-3-Phosphate O-Acyltransferase ,medicine.disease ,Matrix Metalloproteinases ,In vitro ,Gene Expression Regulation, Neoplastic ,Mice, Inbred C57BL ,Disease Models, Animal ,RAW 264.7 Cells ,030104 developmental biology ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Heterografts ,Colorectal Neoplasms ,Signal Transduction - Abstract
Metabolic reprogramming in tumor-associated macrophages (TAM) is associated with cancer development, however, the role of macrophage triglyceride metabolism in cancer metastasis is unclear. Here, we showed that TAMs exhibited heterogeneous expression of abhydrolase domain containing 5 (ABHD5), an activator of triglyceride hydrolysis, with migratory TAMs expressing lower levels of ABHD5 compared with the nonmigratory TAMs. ABHD5 expression in macrophages inhibited cancer cell migration in vitro in xenograft models and in genetic cancer models. The effects of macrophage ABHD5 on cancer cell migration were dissociated from its metabolic function as neither triglycerides nor ABHD5-regulated metabolites from macrophages affected cancer cell migration. Instead, ABHD5 deficiency in migrating macrophages promoted NFκB p65-dependent production of matrix metalloproteinases (MMP). ABHD5 expression negatively correlated with MMP expression in TAMs and was associated with better survival in patients with colorectal cancer. Taken together, our findings show that macrophage ABHD5 suppresses NFκB-dependent MMP production and cancer metastasis and may serve as a prognostic marker in colorectal cancer. Significance: These findings highlight the mechanism by which reduced expression of the metabolic enzyme ABHD5 in macrophages promotes cancer metastasis.
- Published
- 2019
14. Author Correction: Development of a valid Chinese version of the Cumberland Ankle Instability Tool in Chinese-speaking patients with chronic ankle instability disorders
- Author
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Xia Kang, Song Chen, Dong-fa Liao, Wei Wang, Qing-yun Xie, Wei Zheng, and Wei Xu
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medicine.medical_specialty ,Multidisciplinary ,business.industry ,Science ,MEDLINE ,Chinese version ,Physical medicine and rehabilitation ,Chronic ankle instability ,Medicine ,business ,Ankle instability - Published
- 2021
15. Electroacupuncture Alleviates Functional Dyspepsia by Targeting miR-221-3p to Regulate c-kit/SCF and Raf/Erk Signaling
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Xiao-Li Pan, Jianchao Fan, Hao Huang, Yanjun Du, Li Zhou, Xiaoming Zhang, Jia Li, Hongxing Zhang, Yan Cao, Pai-di Xu, and Zhao-xia Kang
- Subjects
Text mining ,business.industry ,Electroacupuncture ,medicine.medical_treatment ,Erk signaling ,medicine ,Cancer research ,business - Abstract
Background: Functional dyspepsia (FD) is a gastrointestinal disorder associated with epigastric pain and fullness, with symptoms such as anxiety and depression. Electroacupuncture (EA) has emerged as a potential therapeutic strategy to treat FD via electrical stimulation at specific acupoints of the body and has previously been demonstrated to be effective in promoting gastric motility and attenuating the symptoms of FD. However, the mechanisms underlying the effect of EA remain elusive.Results: Herein, we constructed an in vivo model of FD in rats and performed EA on FD-induced rats. Preliminary bioinformatic analysis through RNA sequencing revealed that differentially expressed microRNAs exist between non-treated and EA-treated rats subjected to EA. Among them, miR-221-3p was associated with the target gene c-kit, the expression of which is closely linked to interstitial cells of Cajal (ICCs) and gap junction intercellular communication, factors critically implicated in gastrointestinal motility. We further proved that miR-221-3p overexpression aggravated FD, but EA downregulated miR-221-3p to alleviate the symptoms of FD. The mechanisms of action underlying the effect of EA involve an increase in c-kit+ ICCs, upregulation of stem cell factor and connexin 43, and enhanced Raf/Erk signaling.Conclusions: Our findings suggest that EA is an effective method of managing FD by regulating miR-221-3p, the c-kit/stem cell factor axis, and Raf/Erk signaling, reinforcing the strong role of gap junction intercellular communication in gastrointestinal motility.
- Published
- 2021
16. The Effects of Sevoflurane vs. Propofol for Inflammatory Responses in Patients Undergoing Lung Resection: A Meta-Analysis of Randomized Controlled Trials
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Xia Kang, Jing-Li Yuan, Jiaqiang Zhang, Kang Kang, Meng-Rong Miao, Jie Lu, Bing Li, and Wei Zhang
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RD1-811 ,sevoflurane ,Sevoflurane ,Proinflammatory cytokine ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,030202 anesthesiology ,law ,OLV ,medicine ,030212 general & internal medicine ,Original Research ,Lung ,medicine.diagnostic_test ,propofol ,business.industry ,inflammatory response ,respiratory system ,meta-analysis ,medicine.anatomical_structure ,Bronchoalveolar lavage ,Strictly standardized mean difference ,Anesthesia ,Breathing ,Surgery ,business ,Propofol ,medicine.drug - Abstract
Objective: Inflammatory cytokines are increased during one-lung ventilation in patients undergoing lung resection, and this increase can be fatal. Propofol and sevoflurane are the main anesthetics used for these patients. Unfortunately, there is no consensus on the best choice of an anesthetic agent concerning an inflammatory response in patients undergoing lung resection. This meta-analysis aimed to compare the effects of propofol and sevoflurane on the inflammatory response in patients undergoing lung resection.Methods: We searched electronic databases to identify randomized controlled trials comparing the effects of different anesthetics (sevoflurane vs. propofol) on the inflammatory response. The primary outcome concerned the concentration of systemic inflammatory cytokines. The secondary outcomes concerned the concentrations of inflammatory cytokines in the bronchoalveolar lavage (BAL) fluid from the dependent and independent lung. Random effects analysis of the meta-analyses were performed to synthesize the evidence and to assess the concentrations of inflammatory factors in the sevoflurane and propofol groups.Results: Eight trials involving 488 participants undergoing lung resection with one-lung ventilation were included. There was no significant difference in the concentrations of systemic interleukin (IL)-6, IL-10, or tumor necrosis factor α between the sevoflurane and propofol groups. Compared with the propofol group, BAL levels of IL-6 in the dependent ventilated lung were decreased in the sevoflurane group (three trials, 256 participants; standardized mean difference [SMD], −0.51; 95% confidence interval [CI], −0.90 to −0.11; p = 0.01; I2 = 46%). The BAL levels of IL-6 in the independent ventilated lung were also decreased by sevoflurane (four trials, 362 participants; SMD, −0.70; 95% [CI], −0.93 to −0.47; p < 0.00001; I2 = 0%).Conclusions: There was no difference in the systemic inflammatory response between the sevoflurane and propofol groups. However, compared with propofol, sevoflurane can reduce the local alveolar inflammatory response. Additional research is necessary to confirm whether the inflammatory response is direct or indirect.
- Published
- 2021
17. Validation and Prediction of the School Psychological Capital Among Chinese College Students
- Author
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Lisheng Li, Xia Kang, and Yajun Wu
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050109 social psychology ,Student engagement ,Context (language use) ,school psychological capital ,Structural equation modeling ,achievement emotions ,medicine ,Psychology ,0501 psychology and cognitive sciences ,General Psychology ,Original Research ,validation ,05 social sciences ,college students ,050301 education ,Boredom ,Confirmatory factor analysis ,BF1-990 ,Scale (social sciences) ,Capital (economics) ,Anxiety ,medicine.symptom ,academic engagement ,0503 education ,Social psychology - Abstract
This study validated the school psychological capital (PsyCap) scale in the Chinese context and examined the predictive effect of PsyCap resources on academic engagement and achievement emotions. Self-report data for PsyCap resources, student engagement, enjoyment, anxiety, and boredom toward English learning were collected from 1,000 sophomores. Item-level analyses and confirmatory factor analysis were used to verify the validity of the school PsyCap scale, and structural equation modeling was applied to reveal the predictive effect of school PsyCap resources on academic engagement and achievement emotions. Results showed that the school PsyCap scale retained superior psychometric properties. Besides, PsyCap resources were demonstrated to have a positive relationship to academic engagement and enjoyment, and a negative relationship to anxiety and boredom. The effectiveness of the school PsyCap scale was verified among Chinese college students, and besides the traditional predictors, school PsyCap is also critically important for students’ academic engagement and achievement emotions. Limitations and implications are discussed.
- Published
- 2021
18. Images of Microbiology
- Author
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Louise A. Walker, Ali Floyd, Nicola R. Stanley-Wall, Margarita Kalamara, Sarah J. Coulthurst, Karen Stephenson, Geoffrey M. Gadd, Chih-Yu Hsu, Marina Fomina, John Thomas, Megan Bergkessel, Manu De Rycker, Amy Cameron, Ailsa Mackintosh, Lewis Houghton, Thibault Rosazza, Xia Kang, Saria Mansoor, Erin Hardee, Sujatha Manthri, and Helge C. Dorfmueller
- Subjects
medicine.medical_specialty ,Engineering ,business.industry ,medicine ,Medical physics ,business - Abstract
Here you will find a series of images taken by scientists based at the University of Dundee that highlight the microbes they work with. The images in the collection are part of a physical exhibition located at the Dundee Science Centre.
- Published
- 2021
19. Development of a valid Chinese version of the Cumberland Ankle Instability Tool in Chinese-speaking patients with chronic ankle instability disorders
- Author
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Xia Kang, Wei Xu, Wei Zheng, Dong-fa Liao, Wei Wang, Qing-yun Xie, and Song Chen
- Subjects
Adult ,Cross-Cultural Comparison ,Joint Instability ,Male ,Quality of life ,medicine.medical_specialty ,Adolescent ,Intraclass correlation ,Science ,Article ,03 medical and health sciences ,0302 clinical medicine ,Cronbach's alpha ,Surveys and Questionnaires ,Medicine ,Humans ,Psychology ,Author Correction ,Reliability (statistics) ,Language ,030203 arthritis & rheumatology ,030222 orthopedics ,Multidisciplinary ,business.industry ,Middle Aged ,medicine.anatomical_structure ,Standard error ,Outcomes research ,Chronic ankle instability ,Physical therapy ,Female ,Ankle ,business ,Ankle Joint - Abstract
As an effective scale for the condition assessment of patients with chronic ankle instability (CAI), the Cumberland Ankle Instability Tool (CAIT) is the most widely used scale, and its original version is written in English. Therefore, the purpose of our study is to apply the CAIT to Chinese patients and evaluate its responsiveness, reliability, and validity in terms of Chinese patients with CAI. First, we adapted the CAIT into the Chinese edition (CAIT-C), through which cross-cultural adaptation and translation can be carried out in a five-step procedure. Next, recruited patients completed the three periods of the Foot and Ankle Ability Measure (FAAM), CAIT-C, and the Medical Outcomes Study Short-Form 36 (SF-36) scales. Afterward, to assess the responsiveness, reliability, and validity, we calculated the standardized response mean (SRM), effect size (ES), Spearman's correlation coefficient (rs), minimal detectable change (MDC), standard error of measurement (SEM), intraclass correlation coefficient (ICC), and Cronbach’s alpha. Generally, in the use of CAI, 131, 119, and 86 patients favorably completed the three periods of the scales. The CAIT-C was proven to have good test–retest reliability (ICC = 0.930) and fine internal consistency (Cronbach’s alpha = 0.845–0.878). The low-value of MDC (0.04–2.28) and SEM (1.73) show it is possible to detect clinical changes when we take advantage of CAIT-C. Good or moderate correlations (rs = 0.422–0.738) were gained from the physical subscales of the SF-36 and the subscales of the FAAM and the CAIT-C. Fair or poor correlations (rs = 0.003–0.360) were gained between the mental subscales of the SF-36 and the CAIT-C, which sufficiently indicated that the CAIT-C had good validity. Moreover, good responsiveness was observed in the CAIT-C (ES = 1.316, SRM = 1.418). The CAIT-C scale is an effective, valid, and reliable tool to evaluate Chinese CAI patients.
- Published
- 2021
20. Absence of estrogen receptor beta leads to abnormal adipogenesis during early tendon healing by an up‐regulation of PPARγ signalling
- Author
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Xia Kang, Youxing Shi, Gang He, Tianyao Liu, Yang Mingyu, Bian Xuting, Mei Zhou, Yuanyuan Ma, Kanglai Tang, Xiaotang Fan, Jan-Åke Gustafsson, Yunjiao Wang, and Hong Tang
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0301 basic medicine ,Male ,Transcriptional Activation ,medicine.medical_specialty ,Estrogen receptor ,Achilles Tendon ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,Tendon Injuries ,Internal medicine ,medicine ,Adipocytes ,Animals ,Estrogen Receptor beta ,achilles tendon healing ,Estrogen receptor beta ,Achilles tendon ,Wound Healing ,Adipogenesis ,business.industry ,Cartilage ,Stem Cells ,estrogen receptor β ,PPARγ signalling ,Cell Differentiation ,Cell Biology ,Original Articles ,musculoskeletal system ,Tendon ,Up-Regulation ,Mice, Inbred C57BL ,PPAR gamma ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Molecular Medicine ,Original Article ,Stem cell ,business ,tendon‐derived stem cells ,Signal Transduction ,Stem Cell Transplantation - Abstract
Achilles tendon injury is one of the challenges of sports medicine, the aetiology of which remains unknown. For a long time, estrogen receptor β (ERβ) has been known as a regulating factor of the metabolism in many connective tissues, such as bone, muscle and cartilage, but little is known about its role in tendon. Recent studies have implicated ERβ as involved in the process of tendon healing. Tendon‐derived stem cells (TDSCs) are getting more and more attention in tendon physiological and pathological process. In this study, we investigated how ERβ played a role in Achilles tendon healing. Achilles tendon injury model was established to analyse how ERβ affected on healing process in vivo. Cell proliferation assay, Western blots, qRT‐PCR and immunocytochemistry were performed to investigate the effect of ERβ on TDSCs. Here, we showed that ERβ deletion in mice resulted in inferior gross appearance, histological scores and, most importantly, increased accumulation of adipocytes during the early tendon healing which involved activation of peroxisome proliferator‐activated receptor γ (PPARγ) signalling. Furthermore, in vitro results of ours confirmed that the abnormity might be the result of abnormal TDSC adipogenic differentiation which could be partially reversed by the treatment of ERβ agonist LY3201. These data revealed a role of ERβ in Achilles tendon healing for the first time, thereby providing a new target for clinical treatment of Achilles tendon injury.
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- 2019
21. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
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Kang Lu, Yunjiao Wang, Jiqiang Zhang, Yang Mingyu, Kanglai Tang, Guo Yupeng, Bian Xuting, Binghua Zhou, Wan Chen, Miduo Mu, Xia Kang, Mei Zhou, Fan Lai, Min Zhu, Jingtong Lyu, Hong Tang, Youxing Shi, and Gang He
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Male ,0301 basic medicine ,Interleukin-1beta ,exosomes ,Achilles Tendon ,Rats, Sprague-Dawley ,Extracellular matrix ,03 medical and health sciences ,0302 clinical medicine ,Tissue engineering ,Tendon Injuries ,medicine ,Animals ,tendinopathy ,tendon stem cells ,Tissue Inhibitor of Metalloproteinase-3 ,Wound Healing ,Achilles tendon ,Tissue Engineering ,business.industry ,Stem Cells ,Regeneration (biology) ,Cell Differentiation ,Original Articles ,Cell Biology ,musculoskeletal system ,medicine.disease ,Microvesicles ,Extracellular Matrix ,Rats ,Tendon ,Tenomodulin ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Culture Media, Conditioned ,030220 oncology & carcinogenesis ,Molecular Medicine ,Original Article ,Tendinopathy ,business - Abstract
Tendon injuries are common musculoskeletal system disorders in clinical, but the regeneration ability of tendon is limited. Tendon stem cells (TSCs) have shown promising effect on tissue engineering and been used for the treatment of tendon injury. Exosomes that serve as genetic information carriers have been implicated in many diseases and physiological processes, but effect of exosomes from TSCs on tendon injury repair is unclear. The aim of this study is to make clear that the effect of exosomes from TSCs on tendon injury healing. Exosomes were harvested from conditioned culture media of TSCs by a sequential centrifugation process. Rat Achilles tendon tendinopathy model was established by collagenase‐I injection. This was followed by intra‐Achilles‐tendon injection with TSCs or exosomes. Tendon healing and matrix degradation were evaluated by histology analysis and biomechanical test at the post‐injury 5 weeks. In vitro, TSCs treated with interleukin 1 beta were added by conditioned medium including exosomes or not, or by exosomes or not. Tendon matrix related markers and tenogenesis related markers were measured by immunostaining and western blot. We found that TSCs injection and exosomes injection significantly decreased matrix metalloproteinases (MMP)‐3 expression, increased expression of tissue inhibitor of metalloproteinase‐3 (TIMP‐3) and Col‐1a1, and increased biomechanical properties of the ultimate stress and maximum loading. In vitro, conditioned medium with exosomes and exosomes also significantly decreased MMP‐3, and increased expression of tenomodulin, Col‐1a1 and TIMP‐3. Exosomes from TSCs could be an ideal therapeutic strategy in tendon injury healing for its balancing tendon extracellular matrix and promoting the tenogenesis of TSCs.
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- 2019
22. On the mechanism of antibiotic resistance and fecal microbiota transplantation
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Fan Huang, Ling Yang, Jie Yan, and Xia Xia Kang
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Methicillin-Resistant Staphylococcus aureus ,Risk ,medicine.drug_class ,Antibiotics ,02 engineering and technology ,Drug resistance ,Microbiology ,Feces ,Antibiotic resistance ,Drug Resistance, Bacterial ,0502 economics and business ,0202 electrical engineering, electronic engineering, information engineering ,medicine ,Humans ,Computer Simulation ,Pathogen ,biology ,Resistance (ecology) ,Mechanism (biology) ,Probiotics ,Applied Mathematics ,05 social sciences ,Drug Resistance, Microbial ,Bacterial Infections ,General Medicine ,Fecal Microbiota Transplantation ,Models, Theoretical ,biology.organism_classification ,Anti-Bacterial Agents ,Intestines ,Computational Mathematics ,Modeling and Simulation ,020201 artificial intelligence & image processing ,Steady state (chemistry) ,General Agricultural and Biological Sciences ,050203 business & management ,Bacteria - Abstract
Antibiotic resistance is a growing threat to human health and is caused by mainly the overuse of antibiotics in clinical medicine. Clinically, drug resistance emerges after a series of antibiotic treatments, implying that each treatment changes the intestinal flora composition and the accumulations of these changes induce the resistance. But mathematically, this cumulative effect cannot be achieved by a general population model, because the system will return to its pre-treatment state (an isolated steady state) after each cure. Based on the fact that sensitive bacteria and resistant bacteria are similar in most respects except their reactions to antibiotics, we developed a mathematical model with a specific phase-space structure: instead of isolated points, the steady states of this system compose one-dimensional manifolds (line segments). This structure explains the fundamental mechanism of antibiotic resistance: after antibiotic treatment, the system cannot return to the pretreatment healthy steady state but rather slightly moves along the manifold to a different steady state. Each use of antibiotics can change the ratio of resistant to susceptible pathogens in the host. The change the ratio can persist and accumulate, and finally promotes the emergence of antimicrobial resistance. We also assessed key factors (such as pathogen composition, the amount and composition of beneficial bacteria, medication duration and bactericidal rates of drugs) influencing the development of drug resistance. In addition, we clarified how fecal microbiota transplantation affects the treatment of antibiotic-resistant infections. The effect is essentially a transfer towards the healthy state in the phase space. Finally, based on the mechanisms revealed by the mathematical models, we suggested some strategies to delay or prevent the emergence of drug resistance. These findings not only provide a solid theoretical basis for the treatment of antimicrobial resistance, but also inspire clues to the phenomenon of drug resistance.
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- 2019
23. Alteration of Adaptive Immunity in a Colorectal Peritoneal Carcinomatosis Model
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Hongming Miao, Xia Kang, Wei Xiang, Rongchen Shi, Lili Zhang, Jinping Wang, and Fengtian He
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0301 basic medicine ,Colorectal cancer ,business.industry ,peritoneal carcinomatosis ,Intraperitoneal inoculation ,colorectal cancer ,Spleen ,adaptive immunity ,medicine.disease ,Acquired immune system ,Colorectal cancer cell line ,Peritoneal carcinomatosis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,business ,Visceral fat ,CD8 ,Research Paper - Abstract
Colorectal cancer (CRC) usually gives rise to transcoelomic spread and ultimately causes peritoneal carcinomatosis (PC). However, mechanism studies, especially the immunological basis of colorectal PC, are rarely revealed due to lack of a suitable PC model. Here we selected a mouse colorectal cancer cell line MC-38 for intraperitoneal inoculation in the C57BL/6 mice to mimic the development of colorectal PC. We demonstrated that the injected CRC cells preferentially and rapidly migrated and colonized in the visceral fat tissues, but not in other visceral organs. With flow cytometric analysis, we found the proportions of spleen T cells and B cells were not affected by PC progression, while the ratios of blood CD4+ and CD8+ T cells were largely influenced. Especially, the quantity or activity of CD4+ and CD8+ T cells in visceral fats were intimately regulated by PC development. Taken together, we successfully constructed a colorectal PC model in immune-competent mice and revealed the alteration of adaptive immunity in PC development. Our study might potentiate the research and therapy strategies of colorectal PC.
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- 2019
24. Culturable heavy metal-resistant and plant growth promoting bacteria in V-Ti magnetite mine tailing soil from Panzhihua, China.
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Xiumei Yu, Yanmei Li, Chu Zhang, Huiying Liu, Jin Liu, Wenwen Zheng, Xia Kang, Xuejun Leng, Ke Zhao, Yunfu Gu, Xiaoping Zhang, Quanju Xiang, and Qiang Chen
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Medicine ,Science - Abstract
To provide a basis for using indigenous bacteria for bioremediation of heavy metal contaminated soil, the heavy metal resistance and plant growth-promoting activity of 136 isolates from V-Ti magnetite mine tailing soil were systematically analyzed. Among the 13 identified bacterial genera, the most abundant genus was Bacillus (79 isolates) out of which 32 represented B. subtilis and 14 B. pumilus, followed by Rhizobium sp. (29 isolates) and Ochrobactrum intermedium (13 isolates). Altogether 93 isolates tolerated the highest concentration (1000 mg kg(-1)) of at least one of the six tested heavy metals. Five strains were tolerant against all the tested heavy metals, 71 strains tolerated 1,000 mg kg(-1) cadmium whereas only one strain tolerated 1,000 mg kg(-1) cobalt. Altogether 67% of the bacteria produced indoleacetic acid (IAA), a plant growth-promoting phytohormone. The concentration of IAA produced by 53 isolates was higher than 20 µg ml(-1). In total 21% of the bacteria produced siderophore (5.50-167.67 µg ml(-1)) with two Bacillus sp. producing more than 100 µg ml(-1). Eighteen isolates produced both IAA and siderophore. The results suggested that the indigenous bacteria in the soil have beneficial characteristics for remediating the contaminated mine tailing soil.
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- 2014
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25. Biomechanical study of injectable hollow pedicle screws for PMMA augmentation in severely osteoporotic lumbar vertebrae: effect of PMMA distribution and volume on screw stability
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Wei Zheng, Hong-Hua Wu, Da Liu, Qing-yun Xie, Xia Kang, Jiang-jun Zhou, Yang Luo, and Jun Sheng
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Lumbar vertebrae ,Junction area ,03 medical and health sciences ,0302 clinical medicine ,Posterior wall ,Pedicle Screws ,Pilot hole ,Humans ,Polymethyl Methacrylate ,Medicine ,Pedicle screw ,Orthodontics ,030222 orthopedics ,Lumbar Vertebrae ,business.industry ,Bone Cements ,General Medicine ,Pullout strength ,Biomechanical Phenomena ,Prosthesis Failure ,Vertebral body ,medicine.anatomical_structure ,Osteoporosis ,Spinal Fractures ,business ,Cadaveric spasm ,030217 neurology & neurosurgery - Abstract
OBJECTIVEThe purpose of this study was to compare stability of injectable hollow pedicle screws with different numbers of holes using different volumes of polymethylmethacrylate (PMMA) in severely osteoporotic lumbar vertebrae and analyze the relationship between screw stability and distribution and volume of PMMA.METHODSForty-eight severely osteoporotic cadaveric lumbar vertebrae were randomly divided into 3 groups—groups A, B, and C (16 vertebrae per group). The screws used in group A had 4 holes (2 pairs of holes, with the second hole of each pair placed 180° further along the thread than the first). The screws used in group B had 6 holes (3 pairs of holes, placed with the same 180° difference in position). Unmodified conventional screws were used in group C. Each group was randomly divided into subgroups 0, 1, 2, and 3, with different volumes of PMMA used in each subgroup. Type A and B pedicle screws were directly inserted into the vertebrae in groups A and B, respectively, and then different volumes of PMMA were injected through the screws into the vertebrae in subgroups 0, 1, 2, and 3. The pilot hole was filled with different volumes of PMMA followed by insertion of screws in groups C0, C1, C2, and C3. Distributions of PMMA were evaluated radiographically, and axial pull-out tests were performed to measure the maximum axial pullout strength (Fmax).RESULTSRadiographic examination revealed that PMMA surrounded the anterior third of the screws in the vertebral bodies (VBs) in groups A1, A2, and A3; the middle third of screws in the junction area of the vertebral body (VB) and pedicle in groups B1, B2, and B3; and the full length of screws evenly in both VB and pedicle in groups C1, C2, and C3. In addition, in groups A3 and B3, PMMA from each of the screws (left and right) was in contact with PMMA from the other screw and the PMMA was closer to the posterior wall and pedicle than in groups A1, A2, B1, and B2. One instance of PMMA leakage was found (in group B3). Two-way analysis of variance revealed that 2 factors—distribution and volume of PMMA—significantly influenced Fmax (p < 0.05) but that they were not significantly correlated (p = 0.078). The Fmax values in groups in which screws were augmented with PMMA were significantly better than those in groups in which no PMMA was used (p < 0.05).CONCLUSIONSPMMA can significantly improve stability of different injectable pedicle screws in severely osteoporotic lumbar vertebrae, and screw stability is significantly correlated with distribution and volume of PMMA. The closer the PMMA is to the pedicle and the greater the quantity of injected PMMA used, the greater the pedicle screw stability is. Injection of 3.0 mL PMMA through screws with 4 holes (2 pair of holes, with the screws in each pair placed on opposite sides of the screw) produces optimal stability in severely osteoporotic lumbar vertebrae.
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- 2018
26. High-dose versus low-dose local anaesthetic for transversus abdominis plane block after bariatric surgery: a systematic review and meta-analysis
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Xia Kang and Jiaqiang Zhang
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medicine.medical_specialty ,Local anaesthetic ,Transversus Abdominis Plane Block ,business.industry ,Meta-analysis ,Low dose ,medicine ,business ,Surgery - Published
- 2020
27. A novel homozygous exon2 deletion of TRIM32 gene in a Chinese patient with sarcotubular myopathy: A case report and literature review
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Xue-Fan Yu, Jing Miao, Zi-Yi Wang, Yan-Lu Gao, Xiao-jing Wei, and Zhi-xia Kang
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0301 basic medicine ,Male ,Weakness ,Pathology ,medicine.medical_specialty ,Ubiquitin-Protein Ligases ,Case Reports ,Gene mutation ,Tripartite Motif Proteins ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,case report ,Myopathy ,Gene ,TRIM32 ,Sarcotubular myopathy ,lcsh:R5-920 ,Difficulty standing up ,biology ,business.industry ,Homozygote ,General Medicine ,Exons ,Middle Aged ,homozygous deletion ,030104 developmental biology ,Muscular Dystrophies, Limb-Girdle ,biology.protein ,Disease Progression ,Creatine kinase ,medicine.symptom ,business ,lcsh:Medicine (General) ,Novel mutation ,vacuoles ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
Sarcotubular myopathy (STM) is a rare autosomal recessive myopathy caused by TRIM32 gene mutations. It is predominantly characterized by the weakness of the proximal limb and mild to moderate elevation of creatine kinase (CK) levels. In this study, we describe a 50‑year‑old Chinese man who exhibited a proximal-to-distal weakness in the muscles of the lower limbs and who had difficulty standing up from a squat position. The symptoms gradually became more severe. He denied a history of cognitive or cardiological problems. The patient's parents and children were healthy. Histopathological examination revealed dystrophic changes and irregular slit-shaped vacuoles containing amorphous materials. Whole-exome sequencing consisting of protein-encoding regions of 19,396 genes was performed, the results of which identified one novel homozygous 2kb deletion chr9.hg19: g.119460021_119461983del (exon2) in the TRIM32 gene. This was confirmed at the homozygous state with quantitative real-time PCR. Here, we present a Chinese case of STM with one novel mutation in TRIM32 and provide a brief summary of all known pathogenic mutations in TRIM32.
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- 2020
28. Dietary fats suppress the peritoneal seeding of colorectal cancer cells through the TLR4/Cxcl10 axis in adipose tissue macrophages
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Xia Kang, Wei Xiang, Xuan Zhang, Hongming Miao, Dapeng Zhang, Lili Zhang, Jing Yuan, and Rongchen Shi
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0301 basic medicine ,Cancer Research ,Colorectal cancer ,Phagocytosis ,T cell ,Adipose tissue macrophages ,lcsh:Medicine ,Mice, Nude ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Genetics ,medicine ,Animals ,CXCL10 ,Neoplasm Metastasis ,lcsh:QH301-705.5 ,Peritoneal Neoplasms ,Mice, Knockout ,Mice, Inbred BALB C ,business.industry ,Macrophages ,lcsh:R ,food and beverages ,medicine.disease ,Dietary Fats ,Oxaliplatin ,Blockade ,Chemokine CXCL10 ,Toll-Like Receptor 4 ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Biology (General) ,Adipose Tissue ,030220 oncology & carcinogenesis ,TLR4 ,Cancer research ,Colorectal Neoplasms ,business ,medicine.drug - Abstract
Peritoneal carcinomatosis (PC) of colorectal cancer (CRC) is a terminal phase of malignancy with no effective strategies for the prevention of this condition. Here we established PC models in mice by intraperitoneal engraftment of CRC cells and revealed an unexpected role for a high-fat diet (HFD) in preventing metastatic seeding in the visceral fat. Mechanistically, the HFD stimulated the activation of adipose tissue macrophages (ATMs) toward an M1-like phenotype and enhanced ATM tumor phagocytosis in a TLR4-dependent manner. Furthermore, the TLR4–Cxcl10 axis in ATMs promoted T cell recruitment, and M1-like macrophages stimulated T cell activation in tumor-seeded fats. The inhibitory effect of the HFD on tumor seeding was abolished with the ablation of macrophages, inactivation of T cells, or blockade of the TLR4–Cxcl10 axis in macrophages. Finally, we showed that a HFD and conventional chemotherapeutic agents (oxaliplatin or 5-fluorouracil) synergistically improved the survival of tumor-seeded mice. Collectively, our findings demonstrate that peritoneal seeding of CRC can be suppressed by short-term treatment with a HFD in the early phase, providing a novel concept for the management of these patients in the clinic.
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- 2020
29. Individualized positive end-expiratory pressure (PEEP) during one-lung ventilation for prevention of postoperative pulmonary complications in patients undergoing thoracic surgery: A meta-analysis
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Jiaqiang Zhang, Pule Li, Meng-Rong Miao, and Xia Kang
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medicine.medical_specialty ,one-lung ventilation ,Oxygenation index ,Positive-Pressure Respiration ,Postoperative Complications ,Medicine ,Humans ,Positive end-expiratory pressure ,Randomized Controlled Trials as Topic ,business.industry ,lung-protective ventilation strategy ,General Medicine ,Oxygenation ,Perioperative ,respiratory system ,Thoracic Surgical Procedures ,respiratory tract diseases ,Cardiothoracic surgery ,Meta-analysis ,Anesthesia ,Relative risk ,Breathing ,business ,Systematic Review and Meta-Analysis ,circulatory and respiratory physiology ,Research Article ,positive end-expiratory pressure - Abstract
Background: Positive end-expiratory pressure (PEEP) is an important part of the lung protection strategies for one-lung ventilation (OLV). However, a fixed PEEP value is not suitable for all patients. Our objective was to determine the prevention of individualized PEEP on postoperative complications in patients undergoing one-lung ventilation. Method: We searched the PubMed, Embase, and Cochrane and performed a meta-analysis to compare the effect of individual PEEP vs fixed PEEP during single lung ventilation on postoperative pulmonary complications. Our primary outcome was the occurrence of postoperative pulmonary complications during follow-up. Secondary outcomes included the partial pressure of arterial oxygen and oxygenation index during one-lung ventilation. Result: Eight studies examining 849 patients were included in this review. The rate of postoperative pulmonary complications was reduced in the individualized PEEP group with a risk ratio of 0.52 (95% CI:0.37–0.73; P = .0001). The partial pressure of arterial oxygen during the OLV in the individualized PEEP group was higher with a mean difference 34.20 mm Hg (95% CI: 8.92–59.48; P = .0004). Similarly, the individualized PEEP group had a higher oxygenation index, MD: 49.07mmHg, (95% CI: 27.21–70.92; P
- Published
- 2020
30. The impact of goggle-associated harms to health and working status of nurses during management of COVID-19
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Xiao-jiao Pang, Ya-li Zhou, Yan-ru Feng, Xia Kang, Li-min Li, Yong-hua Wang, Ting Chen, Xiao-huan He, Hongming Miao, Li Feng, Jing Lang, Wei Zheng, Xin He, Hao Zhang, and Gao-ming Li
- Subjects
medicine.medical_specialty ,Isolation (health care) ,Pressure injury ,Coronavirus disease 2019 (COVID-19) ,Nurse practitioners ,business.industry ,Nausea ,Incidence (epidemiology) ,Logistic regression ,Rash ,medicine ,Physical therapy ,medicine.symptom ,business - Abstract
SummaryBackgroundTo investigate the impact of goggles on their health and clinical practice during management of patients with COVID-19.Methods231 nurse practitioners were enrolled who worked in isolation region in designated hospitals to admit patients with COVID-19 in China. Demographic data, goggle-associated symptoms and underlying reasons, incidence of medical errors or exposures, the effects of fog in goggles on practice were all collected. Data were stratified and analyzed by age or working experience. Risk factors of goggle-associated medical errors were analyzed by multivariable logistical regression analysis.FindingsGoggle-associated symptoms and foggy goggles widely presented in nurses. The most common symptoms were headache, skin pressure injury and dizziness. Headache, vomit and nausea were significantly fewer reported in nurses with longer working experience while rash occurred higher in this group. The underlying reasons included tightness of goggles, unsuitable design and uncomfortable materials. The working status of nurses with more working experience was less impacted by goggles. 11.3% nurses occurred medical exposures in clinical practice while 19.5% nurses made medical errors on patients. The risk factors for medical errors were time interval before adapting to goggle-associated discomforts, adjusting goggles and headache.InterpretationGoggle-associated symptoms and fog can highly impact the working status and contribute to medical errors during management of COVID-19. Increased the experience with working in PPE through adequate training and psychological education may benefit for relieving some symptoms and improving working status. Improvement of goggle design during productive process was strongly suggested to reduce incidence of discomforts and medical errors.
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- 2020
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31. Exosomes Derived from Bone Marrow Stromal Cells (BMSCs) Enhance Tendon-Bone Healing by Regulating Macrophage Polarization
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Xia Kang, Gang He, Yunjiao Wang, Mei Zhou, Kanglai Tang, Youxing Shi, and Bian Xuting
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Male ,Pathology ,medicine.medical_specialty ,China ,Stromal cell ,Macrophage polarization ,Apoptosis ,Bone Marrow Cells ,macromolecular substances ,030204 cardiovascular system & hematology ,Exosomes ,Mesenchymal Stem Cell Transplantation ,Exosome ,Bone and Bones ,Proinflammatory cytokine ,Tendons ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Tendon Injuries ,medicine ,Animals ,Cells, Cultured ,Cell Proliferation ,Inflammation ,Wound Healing ,Mesenchymal Stromal Cells ,Chemistry ,Macrophages ,Animal Study ,Mesenchymal Stem Cells ,General Medicine ,Macrophage Activation ,M2 Macrophage ,Mice, Inbred C57BL ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Fibrocartilage ,Bone marrow ,Stem cell ,Bone-Patellar Tendon-Bone Grafts - Abstract
BACKGROUND Inflammation after tendon-bone junction injury results in the formation of excessive scar tissue and poor biomechanical properties. Recent research has shown that exosomes derived from bone marrow stromal cells (BMSCs) can modulate inflammation during tissue healing. Thus, our study aimed to enhance tendon-bone healing by use of BMSC-derived exosomes (BMSC-Exos). MATERIAL AND METHODS The mouse tendon-bone reconstruction model was established, and the mice were randomly divided into 3 groups: the control group, the hydrogel group, and the hydrogel+exosome group, with 30 mice in each group. At 7 days, 14 days, and 1 month after surgery, tendon-bone junction samples were harvested, and the macrophage polarization and tendon-bone healing were evaluated based on histology, immunofluorescence, and quantitative RT-PCR (qRT-PCR) analysis. RESULTS In the early phase, we observed significantly higher numbers of M2 macrophages and more anti-inflammatory and chondrogenic-related factors in the hydrogel+BMSC-Exos group compared with the control group and the hydrogel group. The M1 macrophages and related proinflammatory factors decreased. Cell apoptosis decreased in the hydrogel+BMSC-Exos group, while cell proliferation increased; in particular, the CD146+ stem cells substantially increased. At 1 month after surgery, there was more fibrocartilage in the hydrogel+BMSC-Exos group than in the other groups. Biomechanical testing showed that the maximum force, strength, and elastic modulus were significantly improved in the hydrogel+BMSC-Exos group. CONCLUSIONS Our study provides evidence that the local administration of BMSC-Exos promotes the formation of fibrocartilage by increasing M2 macrophage polarization in tendon-to-bone healing, leading to improved biomechanical properties. These findings provide a basis for the potential clinical use of BMSC-Exos in tendon-bone repair.
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- 2020
32. Validation of reported risk factors for disease classification and prognosis in COVID-19: a descriptive and retrospective study
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Li Tan, Xinran Ji, Yongsheng Li, Qi Wang, Xia Kang, Hongming Miao, and Qiongshu Wang
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medicine.medical_specialty ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Internal medicine ,medicine ,Disease classification ,Retrospective cohort study ,Disease ,Typing ,business ,Survival rate ,Viral load ,Procalcitonin - Abstract
Risk indicators viral load (ORF1ab Ct), lymphocyte percentage (LYM%), C-reactive protein (CRP), interleukin-6 (IL-6), procalcitonin (PCT) and lactic acid (LA) in COVID-19 patients have been proposed in recent studies. However, the predictive effects of those indicators on disease classification and prognosis remains largely unknown. We dynamically measured those reported indicators in 132 cases of COVID-19 patients including the moderate-cured (moderated and cured), severe-cured (severe and cured) and critically ill (died). Our data showed that CRP, PCT, IL-6, LYM%, lactic acid and viral load could predict prognosis and guide classification of COVID-19 patients in different degrees. CRP, IL-6 and LYM% were more effective than other three factors in predicting prognosis. For disease classification, CRP and LYM% were sensitive in identifying the types between critically ill and severe (or moderate). Notably, among the investigated factors, LYM% was the only one that could distinguish between the severe and moderate types. Collectively, we concluded that LYM% was the most sensitive and reliable predictor for disease typing and prognosis. During the COVID-19 pandemic, the precise classification and prognosis prediction are critical for saving the insufficient medical resources, stratified treatment and improving the survival rate of critically ill patients. We recommend that LYM% be used independently or in combination with other indicators in the management of COVID-19.
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- 2020
33. A special case of COVID-19 with long duration of viral shedding for 49 days
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Xia Kang, Li Tan, Shangen Zheng, Qiongshu Wang, Fan Yang, Bo Liu, Bo Zhang, Hongming Miao, and Tiantian Yu
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medicine.medical_specialty ,Poor prognosis ,Coronavirus disease 2019 (COVID-19) ,Low toxicity ,business.industry ,Epidemiology ,medicine ,Physiology ,Viral shedding ,business ,Short duration ,Virus - Abstract
SUMMARYProlonged viral shedding is associated with severe status and poor prognosis of COVID-19 patients. Unexpectedly, here we report a non-severe patient with the longest duration of viral shedding. According to the investigation on the clinical and epidemiological information of this case, we concluded that this type of virus might have a low toxicity and transmissibility, but have a prolonged infective ability and was hardly to be eliminated in the body with regular therapy. However, infusion of plasma from recovered patients showed high efficiency in elimination of this virus. Our findings might shed light on the management of COVID-19.
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- 2020
34. Plasma therapy cured a COVID-19 patient with long duration of viral shedding for 49 days: The clinical features, laboratory tests, plasma therapy, and implications for public health management
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Bo Zhang, Hongming Miao, Tiantian Yu, Qiongshu Wang, Xia Kang, Shangen Zheng, Fan Yang, Bo Liu, and Li Tan
- Subjects
2019-20 coronavirus outbreak ,medicine.medical_specialty ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Public health ,MEDLINE ,Internal medicine ,medicine ,Viral shedding ,Plasma therapy ,business ,Short duration ,Letter to the Editor - Published
- 2020
35. Lactic acid promotes metastatic niche formation in bone metastasis of colorectal cancer
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Juan Wu, Wei Wang, Yi-na Zhang, Da Liu, Jing Zhao, Liu Xilin, Wei Zheng, Wei Xu, Hong-Hua Wu, Li-juan Ye, Li-ting Wang, Jun Sheng, Zi-chen Gong, Jin Qian, and Xia Kang
- Subjects
0301 basic medicine ,CD4-Positive T-Lymphocytes ,Male ,lcsh:Medicine ,Osteoclasts ,Biochemistry ,Phosphatidylinositol 3-Kinases ,0302 clinical medicine ,Cell Movement ,Tumor Microenvironment ,Cyclic AMP Response Element-Binding Protein ,Cells, Cultured ,lcsh:Cytology ,Chemistry ,TOR Serine-Threonine Kinases ,CXCL10 ,Bone metastasis ,Cell Differentiation ,Cadherins ,Blot ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Collagen ,Colorectal Neoplasms ,Bone Neoplasms ,Bone resorption ,03 medical and health sciences ,Osteoclast ,medicine ,Cell Adhesion ,Animals ,Lactic Acid ,Osteoclast precursors ,lcsh:QH573-671 ,Molecular Biology ,PI3K/AKT/mTOR pathway ,Tumor microenvironment ,Cadherin-11 ,Research ,lcsh:R ,Cell Biology ,medicine.disease ,PI3K-AKT pathway ,Colorectal cancer ,Coculture Techniques ,Chemokine CXCL10 ,Mice, Inbred C57BL ,030104 developmental biology ,Apoptosis ,Cancer research ,Proto-Oncogene Proteins c-akt - Abstract
Background To investigate the effect of lactic acid (LA) on the progression of bone metastasis from colorectal cancer (CRC) and its regulatory effects on primary CD115 (+) osteoclast (OC) precursors. Methods The BrdU assay, Annexin-V/PI assay, TRAP staining and immunofluorescence were performed to explore the effect of LA on the proliferation, apoptosis and differentiation of OC precursors in vitro and in vivo. Flow cytometry was performed to sort primary osteoclast precursors and CD4(+) T cells and to analyze the change in the expression of target proteins in osteoclast precursors. A recruitment assay was used to test how LA and Cadhein-11 regulate the recruitment of OC precursors. RT-PCR and Western blotting were performed to analyze the changes in the mRNA and protein expression of genes related to the PI3K-AKT pathway and profibrotic genes. Safranin O-fast green staining, H&E staining and TRAP staining were performed to analyze the severity of bone resorption and accumulation of osteoclasts. Results LA promoted the expression of CXCL10 and Cadherin-11 in CD115(+) precursors through the PI3K-AKT pathway. We found that CXCL10 and Cadherin-11 were regulated by the activation of CREB and mTOR, respectively. LA-induced overexpression of CXCL10 in CD115(+) precursors indirectly promoted the differentiation of osteoclast precursors through the recruitment of CD4(+) T cells, and the crosstalk between these two cells promoted bone resorption in bone metastasis from CRC. On the other hand, Cadherin-11 mediated the adhesion between osteoclast precursors and upregulated the production of specific collagens, especially Collagen 5, which facilitated fibrotic changes in the tumor microenvironment. Blockade of the PI3K-AKT pathway efficiently prevented the progression of bone metastasis caused by lactate. Conclusion LA promoted metastatic niche formation in the tumor microenvironment through the PI3K-AKT pathway. Our study provides new insight into the role of LA in the progression of bone metastasis from CRC.
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- 2020
36. Immunosenescence evaluation of peripheral blood lymphocyte subsets in 957 healthy adults from 20 to 95 years old
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Li-Li Cai, Jihong Yu, Yu-Ru Li, Xin-Li Deng, Chao Wu, Xingchi Chen, Heqiang Sun, and Xia Kang
- Subjects
Male ,Aging ,Immunosenescence ,Lymphocyte ,Population ,CD4-CD8 Ratio ,Biochemistry ,Endocrinology ,Immune system ,T-Lymphocyte Subsets ,Genetics ,medicine ,Humans ,Cytotoxic T cell ,Lymphocyte Count ,education ,Molecular Biology ,Aged ,Aged, 80 and over ,education.field_of_study ,business.industry ,CD28 ,Cell Biology ,Flow Cytometry ,Lymphocyte Subsets ,Killer Cells, Natural ,medicine.anatomical_structure ,Peripheral blood lymphocyte ,Immunology ,Female ,business ,CD8 - Abstract
Immunosenescence is characterized by an age-related decline in immune system function. Major efforts have been made to identify changes in peripheral blood lymphocyte subsets accompanying immunosenescence in elderly adults. However, the change trends of some lymphocyte subsets with age are still controversial, and populations of advanced ages, such as people in their 80s or 90s, have not yet been thoroughly investigated. To provide further insight, we recruited 957 healthy donors without certain diseases with ages ranging from 20 to 95 years. Peripheral lymphocyte subsets, including T cells, CD4 T cells, CD8 T cells, B cells and NK cells, and the CD4/CD8 ratio were measured by flow cytometry. Additionally, regulatory CD4 T cells with inhibitory functions marked by CD3+CD4+CD25hi and the expression of the costimulatory molecule CD28 on CD8 T cells were evaluated. Sex was considered at the same time. The data indicated that in elderly people, peripheral T (p
- Published
- 2022
37. Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression
- Author
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Xuan Zhang, Chunmeng Shi, Along Hou, Shenglan Shang, Rongchen Shi, Lili Zhang, Songtao Yu, Lixia Gan, Yuanyin Zhao, Hongming Miao, Huan Luo, Yingzhe Liu, Peng Chen, Yongsheng Li, Rui Wang, Jinyu Zhang, Ma Yue, Xia Kang, Wei Yang, Wei Xiang, Houjie Liang, Kun Zhao, and Fengtian He
- Subjects
Male ,0301 basic medicine ,Colorectal cancer ,medicine.medical_treatment ,General Physics and Astronomy ,CD8-Positive T-Lymphocytes ,Receptor, Cannabinoid, CB2 ,Mice ,Neoplasms ,Cannabinoid receptor type 2 ,Macrophage ,Receptor ,skin and connective tissue diseases ,lcsh:Science ,Mice, Knockout ,Mice, Inbred BALB C ,Multidisciplinary ,Chemistry ,Middle Aged ,Disease Progression ,Female ,lipids (amino acids, peptides, and proteins) ,hormones, hormone substitutes, and hormone antagonists ,Science ,Primary Cell Culture ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,stomatognathic system ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Aged ,Macrophages ,Cancer ,General Chemistry ,Lipid Metabolism ,medicine.disease ,Monoacylglycerol Lipases ,Mice, Inbred C57BL ,Toll-Like Receptor 4 ,Monoacylglycerol lipase ,RAW 264.7 Cells ,030104 developmental biology ,Tumor progression ,Cancer research ,lcsh:Q ,Cannabinoid - Abstract
Metabolic reprogramming greatly contributes to the regulation of macrophage activation. However, the mechanism of lipid accumulation and the corresponding function in tumor-associated macrophages (TAMs) remain unclear. With primary investigation in colon cancer and confirmation in other cancer models, here we determine that deficiency of monoacylglycerol lipase (MGLL) results in lipid overload in TAMs. Functionally, macrophage MGLL inhibits CB2 cannabinoid receptor-dependent tumor progression in inoculated and genetic cancer models. Mechanistically, MGLL deficiency promotes CB2/TLR4-dependent macrophage activation, which further suppresses the function of tumor-associated CD8+ T cells. Treatment with CB2 antagonists delays tumor progression in inoculated and genetic cancer models. Finally, we verify that expression of macrophage MGLL is decreased in cancer tissues and positively correlated with the survival of cancer patients. Taken together, our findings identify MGLL as a switch for CB2/TLR4-dependent macrophage activation and provide potential targets for cancer therapy., Tumour associated macrophages (TAMs) have an altered lipid metabolism. Here the authors show that downregulation of monoacylglycerols lipase MGLL in TAMs induces lipid accumulation and tumor progression by polarizing TAMs toward tumor-promoting through activation of cannabinoid receptor CB2.
- Published
- 2018
38. SPC24 promotes osteosarcoma progression by increasing EGFR/MAPK signaling
- Author
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Kai Jiang, Jia Xu, Feixing Zhao, Mengchen Yin, Zhengwang Sun, Da Liu, Qunfeng Guo, Xia Kang, Wei Zheng, and Jun Sheng
- Subjects
musculoskeletal diseases ,0301 basic medicine ,Gene knockdown ,Cadherin ,Cell growth ,Chemistry ,Erk signaling ,E-cadherin ,Ras/Raf/MEK/ERK signal pathway ,medicine.disease ,03 medical and health sciences ,Spindle checkpoint ,030104 developmental biology ,0302 clinical medicine ,Oncology ,osteosarcoma ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Osteosarcoma ,Tumor growth ,EGFR/MAPK signaling ,SPC24 ,Research Paper - Abstract
In this study, we investigated the role of the spindle checkpoint protein SPC24 in osteosarcoma progression. SPC24 knockdown in 143B and U2OS osteosarcoma cells decreased cell growth, survival and invasiveness. The SPC24 knockdown cells also exhibited low EGFR, Ras and phospho-ERK levels and high E-cadherin levels, suggesting inhibition of EGFR/Ras/ERK signaling and epithelial-to-mesenchymal transitioning. Xenografted SPC24 knockdown osteosarcoma cells showed reduced tumor growth in nude mice with decreased EGFR and phospho-ERK levels and increased E-cadherin levels. By contrast, human osteosarcoma tissue samples showed high SPC24 and phospho-ERK levels and low E-cadherin levels. These results suggest SPC24 promotes osteosarcoma progression by increasing EGFR/Ras/ERK signaling.
- Published
- 2017
39. Oleanolic acid prevents cartilage degeneration in diabetic mice via PPARγ associated mitochondrial stabilization
- Author
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Qing-yun Xie, Wei Zheng, Ken Chen, Jinbiao Liu, Zhikui Yang, Xia Kang, and Jun Sheng
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Biophysics ,SOD2 ,Osteoarthritis ,Protective Agents ,Biochemistry ,Diabetes Mellitus, Experimental ,Diabetes Complications ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Oleanolic Acid ,Molecular Biology ,Cartilage degeneration ,Oleanolic acid ,Cells, Cultured ,Membrane Potential, Mitochondrial ,030203 arthritis & rheumatology ,Membrane potential ,Cartilage ,Diabetic mouse ,Cell Biology ,medicine.disease ,Mitochondria ,Mice, Inbred C57BL ,PPAR gamma ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Apoptosis ,Hyperglycemia ,Cartilage Diseases - Abstract
Hyperglycemia-induced cartilage degeneration induces osteoarthritis (OA). Since oleanolic acid (OLA) have several pharmacological effects such as anti-inflammatory, anti-oxidant, we hypothesized it possesses protection against high glucose injured cartilage. We now report that OLA decreased type X collagen and reversed the cartilage degeneration in growth plate from db/db mice. OLA increased type Ⅱ collagen expression in a concentration-dependent manner (10-50 μΜ) in high glucose-treated chondrocytes. OLA prevented the high glucose induced cell injury and decreased the level of MMP-13, PGE2 and IL-6 due to decreasing mitochondrial membrane potential and stimulated the ATP production. Moreover, OLA treatment inhibited apoptosis. And the reversed SOD2 expression and activity may be ascribed to decreased SOD2 protein degradation by OLA treatment, via PPPAγ. In conclusion, OLA protected against the high-glucose-induced cartilage injury via PPARγ/SOD2 pathway.
- Published
- 2017
40. CT-based morphometric analysis of the occipital condyle: focus on occipital condyle screw insertion
- Author
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Jade He, Zhihai Zhang, Xia Kang, Nozomu Inoue, Alejandro A. Espinoza Orías, Howard S. An, and Jinsong Zhou
- Subjects
Adult ,Male ,Models, Anatomic ,Bone Screws ,Hypoglossal canal ,Condyle ,03 medical and health sciences ,Imaging, Three-Dimensional ,0302 clinical medicine ,Humans ,Medicine ,Computer Simulation ,030212 general & internal medicine ,Aged ,Fixation (histology) ,Observer Variation ,Orthodontics ,Sex Characteristics ,business.industry ,Occipital bone ,Reproducibility of Results ,Occiput ,Equipment Design ,General Medicine ,Anatomy ,Middle Aged ,Occipital condyle ,Sagittal plane ,medicine.anatomical_structure ,Occipital Bone ,Feasibility Studies ,Female ,Tomography, X-Ray Computed ,Cadaveric spasm ,business ,Software ,030217 neurology & neurosurgery - Abstract
OBJECTIVE The segmental occipital condyle screw (OCS) is an alternative fixation technique in occipitocervical fusion. A thorough morphological study of the occipital condyle (OC) is critical for OCS placement. The authors set out to introduce a more precise CT-based method for morphometric analysis of the OC as it pertains to the placement of the segmental OCS, and they describe a novel preoperative simulation method for screw placement. Two new clinically relevant parameters, the height available for the OCS and the warning depth, are proposed. METHODS CT data sets from 27 fresh-frozen human cadaveric occipitocervical spines were used. All measurements were performed using a commercially available 3D reconstruction software package. The length, width, and sagittal angle of the condyle were measured in the axial plane at the base of the OC. The height of the OC and the height available for the segmental OCS were measured in the reconstructed oblique sagittal plane, fitting the ideal trajectory of the OCS recommended in the literature. The placement of a 3.5-mm-diameter screw that had the longest length of bicortical purchase was simulated into the OC in the oblique sagittal plane, with the screw path not being blocked by the occiput and not violating the hypoglossal canal cranially or the atlantooccipital joint caudally. The length of the simulated screw was recorded. The warning depth was measured as the shortest distance from the entry point of the screw to the posterior border of the hypoglossal canal. RESULTS The mean length and width of the OC were found to be larger in males: 22.2 ± 1.7 mm and 12.1 ± 1.0 mm, respectively, overall (p < 0.0001 for both). The mean sagittal angle was 28.0° ± 4.9°. The height available for the OCS was significantly less than the height of the OC (6.2 ± 1.3 mm vs 9.4 ± 1.5 mm, p < 0.0001). The mean screw length (19.3 ± 1.9 mm) also presented significant sex-related differences: male greater than female (p = 0.0002). The mean warning depth was 7.5 ± 1.7 mm. In 7.4% of the samples, although the height of the OC was viable, the height available for the OCS was less than 4.5 mm, thus making screw placement impractical. For these cases, a new preoperative simulation method of the OCS placement was proposed. In 92.6% of the samples that could accommodate a 3.5-mm-diameter screw, 24.0% showed that the entry point of the simulated screw was covered by a small part of the C-1 posterosuperior joint rim. CONCLUSIONS The placement of the segmental OCS is feasible in most cases, but a thorough preoperative radiological analysis is essential and cannot be understated. The height available for the OCS is a more clinically relevant and precise parameter than the height of the OC to enable proper screw placement. The warning depth may be helpful for the placement of the OCS.
- Published
- 2016
41. Plasma metabolite profiling reveals potential biomarkers of giant cell tumor of bone by using NMR-based metabolic profiles: A cross-sectional study
- Author
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Wei Wang, Wei Xu, Xia Kang, Juan Wu, Jun Sheng, Qingyun Xie, Liu Xilin, Da Liu, and Wei Zheng
- Subjects
Adult ,Male ,Magnetic Resonance Spectroscopy ,Observational Study ,Bone Neoplasms ,Bioinformatics ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Metabolomics ,medicine ,Humans ,Human Metabolome Database ,030212 general & internal medicine ,KEGG ,Giant Cell Tumor of Bone ,business.industry ,Case-control study ,biomarkers ,Lipid metabolism ,General Medicine ,Middle Aged ,GCT ,medicine.disease ,metabolomics ,Metabolic pathway ,Cross-Sectional Studies ,Giant cell ,030220 oncology & carcinogenesis ,Case-Control Studies ,Female ,business ,Giant-cell tumor of bone ,Research Article - Abstract
Giant cell tumor (GCT) of bone is a locally aggressive bone tumor, which accounts for 4% to 5% of all primary bone tumors. At present, the early diagnosis and postoperative recurrence monitoring are still more difficult due to the lack of effective biomarkers in GCT. As an effective tool, metabolomics has played an essential role in the biomarkers research of many tumors. However, there has been no related study of the metabolomics of GCT up to now. The purpose of this study was to identify several key metabolites as potential biomarkers for GCT by using nuclear magnetic resonance (NMR)-based metabolic profiles. Patients with GCT in our hospital were recruited in this study and their plasma was collected as the research sample, and plasma collected from healthy subjects was considered as the control. NMR was then utilized to detect all samples. Furthermore, based on correlation coefficients, variable importance for the projection values and P values of metabolites obtained from multidimensional statistical analysis, the most critical metabolites were selected as potential biomarkers of GCT. Finally, relevant metabolic pathways involved in these potential biomarkers were determined by database retrieval, based on which the metabolic pathways were plotted. Finally, 28 GCT patients and 26 healthy volunteers agreed to participate in the study. In the multidimensional statistical analysis, all results showed that there was obvious difference between the GCT group and the control group. Ultimately, 18 metabolites with significant differences met the selection condition, which were identified as potential biomarkers. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) and Human Metabolome Database (HMD) database searching and literature review, these metabolites were found to be mainly correlated with glucose metabolism, fat metabolism, amino acid metabolism, and intestinal microbial metabolism. These metabolic disorders might, in turn, reflect important pathological processes such as proliferation and migration of tumor cells and immune escape in GCT. Our work showed that these potential biomarkers identified appeared to have early diagnostic and relapse monitoring values for GCT, which deserve to be further investigated. In addition, it also suggested that metabolomics profiling approach is a promising screening tool for the diagnosis and relapse monitoring of GCT patients.
- Published
- 2019
42. Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway
- Author
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Yang Mingyu, Min Zhu, Gang He, Chengsong Yuan, Miduo Mu, Xia Kang, Kanglai Tang, Wan Chen, Bian Xuting, Jingtong Lyu, Hong Tang, Mei Zhou, Binghua Zhou, Yunjiao Wang, Jiqiang Zhang, and Youxing Shi
- Subjects
0301 basic medicine ,Male ,Smad5 Protein ,Physiology ,Cellular differentiation ,Clinical Biochemistry ,Bioinformatics ,Achilles Tendon ,Smad1 Protein ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Sirius Red ,Cells, Cultured ,Aspirin ,Wound Healing ,business.industry ,Regeneration (biology) ,Stem Cells ,Anti-Inflammatory Agents, Non-Steroidal ,Cell Differentiation ,Cell Biology ,medicine.disease ,Tendon ,Growth Differentiation Factors ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,GDF11 ,Bone Morphogenetic Proteins ,Tendinopathy ,Stem cell ,business ,medicine.drug ,Signal Transduction - Abstract
Tendinopathy is a common musculoskeletal system disorder in sports medicine, but regeneration ability of injury tendon is limited. Tendon stem cells (TSCs) have shown the definitive treatment evidence for tendinopathy and tendon injuries due to their tenogenesis capacity. Aspirin, as the representative of nonsteroidal anti-inflammatory drugs for its anti-inflammatory and analgestic actions, has been commonly used in treating tendinopathy in clinical, but the effect of aspirin on tenogenesis of TSCs is unclear. We hypothesized that aspirin could promote injury tendon healing through inducing TSCs tenogenesis. The aim of the present study is to make clear the effect of aspirin on TSC tenogenesis and tendon healing in tendinopathy, and thus provide new treatment evidence and strategy of aspirin for clinical practice. First, TSCs were treated with aspirin under tenogenic medium for 3, 7, and 14 days. Sirius Red staining was performed to observe the TSC differentiation. Furthermore, RNA sequencing was utilized to screen out different genes between the induction group and aspirin treatment group. Then, we identified the filtrated molecules and compared their effect on tenogenesis and related signaling pathway. At last, we constructed the tendinopathy model and compared biomechanical changes after aspirin intake. From the results, we found that aspirin promoted tenogenesis of TSCs. RNA sequencing showed that growth differentiation factor 6 (GDF6), GDF7, and GDF11 were upregulated in induction medium with the aspirin group compared with the induction medium group. GDF7 increased tenogenesis and activated Smad1/5 signaling. In addition, aspirin increased the expression of TNC, TNMD, and Scx and biomechanical properties of the injured tendon. In conclusion, aspirin promoted TSC tenogenesis and tendinopathy healing through GDF7/Smad1/5 signaling, and this provided new treatment evidence of aspirin for tendinopathy and tendon injuries.
- Published
- 2019
43. Validation of Predictors of Disease Severity and Outcomes in COVID-19 Patients: A Descriptive and Retrospective Study
- Author
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Gao-ming Li, Qi Wang, Yongsheng Li, Li Tan, Qiongshu Wang, Hongming Miao, Xia Kang, and Xinran Ji
- Subjects
lymphocytes ,medicine.medical_specialty ,Adolescent ,Coronavirus disease 2019 (COVID-19) ,Critical Illness ,disease classification ,Disease ,Severity of Illness Index ,Article ,Procalcitonin ,Disease severity ,Internal medicine ,Humans ,Medicine ,Retrospective Studies ,biology ,Interleukin-6 ,SARS-CoV-2 ,business.industry ,C-reactive protein ,COVID-19 ,Disease classification ,Retrospective cohort study ,General Medicine ,C-Reactive Protein ,biology.protein ,prognosis ,business ,Viral load - Abstract
Background The severity and outcome of COVID-19 cases has been associated with percentage of circulating lymphocytes (LYM%), levels of C-reactive protein (CRP), interleukin 6 (IL-6), procalcitonin (PCT), lactic acid (LA) and viral load (ORF1ab Ct). However, the predictive power of each of these indicators in disease classification and prognosis remains largely unclear. Methods we retrospectively collected information on the above parameters in 142 patients with COVID-19, stratifying them by survival or disease severity. Findings CRP, PCT, IL-6, LYM% and ORF1ab Ct were significantly altered between survivors and non-survivors. LYM%, CRP and IL-6 were the most sensitive and reliable factors in distinguishing between survivors and non-survivors. These indicators were significantly different between critically ill and severe/moderate patients. Only LYM% levels were significantly different between severe and moderate types. Among all the investigated indicators, LYM% was the most sensitive and reliable in discriminating between critically ill, severe and moderate types, and between survivors and non-survivors. Conclusions CRP, PCT, IL-6, LYM% and ORF1ab Ct, but not LA, could predict prognosis and guide classification of COVID-19 patients. LYM% was the most sensitive and reliable predictor for disease typing and prognosis. We recommend that LYM% be further investigated in the management of COVID-19., Graphical Abstract, Highlights: (1) Inflammatory cytokines, lymphocytes and viral load predict prognosis in COVID-19 patients. (2) Inflammatory cytokines, lymphocytes and viral load indicate disease severity. (3) Lymphocytes and C-reactive protein distinguish between severe and moderate types. (4) Lymphocyte count is the most effective indicator of disease severity and prognosis.
- Published
- 2020
44. Biomechanical comparison of pedicle screw augmented with different volumes of polymethylmethacrylate in osteoporotic and severely osteoporotic cadaveric lumbar vertebrae: an experimental study
- Author
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Xia Kang, Qing-yun Xie, Cai-ru Wang, Wei Zheng, Bo Zhang, Wei Lei, Da Liu, and Jiang-jun Zhou
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,macromolecular substances ,Lumbar vertebrae ,Neurosurgical Procedures ,03 medical and health sciences ,0302 clinical medicine ,Pedicle Screws ,Cadaver ,Humans ,Polymethyl Methacrylate ,Medicine ,Orthopedics and Sports Medicine ,Pedicle screw ,Orthodontics ,030222 orthopedics ,Lumbar Vertebrae ,business.industry ,Bone Cements ,technology, industry, and agriculture ,Pullout strength ,musculoskeletal system ,equipment and supplies ,Surgery ,medicine.anatomical_structure ,Significant positive correlation ,Osteoporosis ,Female ,Neurology (clinical) ,business ,Cadaveric spasm ,030217 neurology & neurosurgery - Abstract
Background Context Polymethylmethacrylate (PMMA) is widely used for pedicle screw augmentation in osteoporosis. Intriguingly, there have been no biomechanical comparisons of the stability of pedicle screws augmented with different volumes of PMMA or studies of the relationship between screw stability and volume of PMMA, especially in different degrees of osteoporosis. Purpose The purposes of the study reported here were to compare screw stability by different volumes of PMMA augmentation, to analyze the relationship between screw stability and PMMA volume, and to make a preliminary determination of the optimum volume of PMMA augmentation for different degrees of osteoporosis. Study Design This study is a biomechanical comparison of pedicle screws augmented with various volumes of PMMA in cadaveric lumbar vertebrae. Methods Thirty-six pedicles from 18 osteoporotic lumbar vertebrae were randomly divided into groups A0 through A5, and 36 pedicles from 18 severely osteoporotic lumbar vertebrae were randomly divided into groups B0 through B5. A different volume of PMMA was injected into each one of groups A0 through A5 (0, 0.5, 1.0, 1.5, 2.0, and 2.5 mL, respectively) and into each one of groups B0 through B5 (0, 1.0, 1.5, 2.0, 2.5, and 3.0 mL, respectively), and then pedicle screws were inserted in all vertebrae. After complete solidification of the PMMA, we examined pedicle X-rays, performed axial pullout tests, and determined the maximum axial pullout strength (F max ) for all samples. Results No PMMA was found around the screws in groups A0 and B0. In groups A1 to A5 and B1 to B5, screws were wrapped by gradually increasing amounts of PMMA. There was no PMMA leakage or screw malpositioning in any samples. The F max in groups A1 through A5 increased by 32.40%, 64.42%, 116.02%, 174.07%, and 207.42%, respectively, compared with that in group A0. There were no significant differences in F max between groups A0 and A1, A1 and A2, A2 and A3, A3 and A4, and A4 and A5 (p>.05), but there were significant differences in F max between any other two groups (p max in groups B1 through B5 increased by 23.48%, 48.40%, 106.60%, 134.73%, and 210.04%, respectively, compared with that in group B0. There were no significant differences in F max between groups B0 and B1, B0 and B2, B1 and B2, B2 and B3, B3 and B4 (p>.05), but there were significant differences in F max between any other two groups (p max and volume of PMMA in both osteoporotic and severely osteoporotic lumbar vertebrae (p Conclusions Polymethylmethacrylate can significantly enhance stability of pedicle screws in both osteoporotic and severely osteoporotic lumbar vertebrae. There is a significant positive correlation between screw stability and volume of PMMA. Within a certain range, nevertheless, increasing the volume of PMMA does not significantly improve screw stability. We suggest that 1.5 and 3 mL, respectively, are the volumes of injected PMMA that will optimize pedicle screw stability in osteoporotic and severely osteoporotic lumbar vertebrae.
- Published
- 2016
45. miR-1260b is a Potential Prognostic Biomarker in Colorectal Cancer
- Author
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Quan-Lin Guan, Hong-Xia Kang, Deng-Rui Liu, Lei Jiang, and Ming-Tai Gao
- Subjects
0301 basic medicine ,Oncology ,Adult ,Male ,medicine.medical_specialty ,Colorectal cancer ,Rectum ,Kaplan-Meier Estimate ,Biology ,Malignancy ,Real-Time Polymerase Chain Reaction ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Lab/In Vitro Research ,Internal medicine ,microRNA ,Intestinal Neoplasms ,medicine ,Biomarkers, Tumor ,Humans ,Survival analysis ,Aged ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Survival Analysis ,digestive system diseases ,MicroRNAs ,030104 developmental biology ,Real-time polymerase chain reaction ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Lymphatic Metastasis ,Multivariate Analysis ,Female ,Colorectal Neoplasms - Abstract
Background Colorectal cancer (CRC) mainly refers to colon and rectum cancer, which is the most common gastrointestinal malignant tumor. MicroRNAs (miRNAs) in tumors participate in multiple processes of malignancy development, including cell differentiation, proliferation, invasion, and metastasis. In this study we explored the relationship of miR-1260b abnormal expression with clinical pathological features in CRC patients. Material/Methods The expression of miR-1260b was detected by real-time quantitative polymerase chain reaction (real-time PCR) in 120 cases of CRC tissues. The correlation of miR-1260b expression with the clinicopathologic features of CRC was analyzed by SPSS 21.0 statistical software. The Kaplan-Meier method was used for survival analysis. Cox regression analyses were conducted to determine whether miR-1260b was an independent predictor of survival for CRC patients. Results The miR-1260b expression in CRC was significantly higher than the expression levels in the corresponding para-carcinoma tissues (P0.05). The high miR-1260b expression patients survived for shorter times than those CRC patients with low miR-1260b expression (P
- Published
- 2016
46. Macrophage TCF-4 co-activates p65 to potentiate chronic inflammation and insulin resistance in mice
- Author
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Xia Kang, Bo Zhang, Wei Xiang, Da Liu, Lixia Gan, Hongming Miao, Qing-yun Xie, Wei Zheng, Along Hou, and Rui Wang
- Subjects
Lipopolysaccharides ,Male ,0301 basic medicine ,medicine.medical_specialty ,Lipopolysaccharide ,Inflammation ,White adipose tissue ,Biology ,Diet, High-Fat ,Systemic inflammation ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Transcription Factor 4 ,Insulin resistance ,Immune system ,Internal medicine ,medicine ,Animals ,Humans ,Cells, Cultured ,Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ,Macrophages ,Transcription Factor RelA ,Interleukin ,General Medicine ,medicine.disease ,Mice, Inbred C57BL ,030104 developmental biology ,Endocrinology ,chemistry ,Chronic Disease ,Cytokines ,Insulin Resistance ,medicine.symptom - Abstract
Transcription factor 4 (TCF-4) was recently identified as a candidate gene for the cause of type 2 diabetes, although the mechanisms have not been fully elucidated. In the present study, we demonstrated that the TCF-4 transgene in macrophages aggravated high-fat diet (HFD)-induced insulin resistance and chronic inflammation, characterized by the elevation of proinflammatory cytokines in the blood, liver and white adipose tissue, as well as a proinflammatory profile of immune cells in visceral fats in mice. Mechanistically, TCF-4 functioned as a co-activator of p65 to amplify the saturated free fatty acid (FFA)-stimulated promoter activity, mRNA transcription and secretion of proinflammatory cytokines in primary macrophages. Blockage of p65 with a specific interfering RNA or inhibitor could prevent TCF-4-enhanced expression of proinflammatory cytokines in FFA/lipopolysaccharide-treated primary macrophages. The p65 inhibitor could abolish macrophage TCF-4 transgene-aggravated systemic inflammation, glucose intolerance and insulin resistance in HFD-treated mice. In addition, we demonstrated that the mRNA expression of TCF-4 in the peripheral blood monocytes from humans was positively correlated to the levels of interleukin (IL)-1β, tumour necrosis factor α, IL-6 and fasting plasma glucose. In summary, we identified TCF-4 as a co-activator of p65 in the potentiation of proinflammatory cytokine production in macrophages and aggravation of HFD-induced chronic inflammation and insulin resistance in mice.
- Published
- 2016
47. Dysregulated ICOS+ proinflammatory and suppressive regulatory T cells�in patients with rheumatoid arthritis
- Author
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Xin Li, Xia Kang, Hongxia Wang, Xiaofeng Yin, Weinan Lai, Haixia Li, Yu‑Rong Qiu, and Shuai Chu
- Subjects
030203 arthritis & rheumatology ,Cancer Research ,Oncogene ,business.industry ,medicine.medical_treatment ,Interleukin ,Inflammation ,General Medicine ,medicine.disease_cause ,Molecular medicine ,Autoimmunity ,Proinflammatory cytokine ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Cytokine ,Immunology and Microbiology (miscellaneous) ,Immunology ,Medicine ,medicine.symptom ,business ,030215 immunology - Abstract
Regulatory T cells (Tregs) serve an important role in the pathogenesis of rheumatoid arthritis (RA) by regulating autoimmunity and inflammation. Humans and mice contain inducible T-cell costimulator-positive (ICOS+) Tregs, although their role in RA is unclear. A total of 33 patients with RA and 17 normal control (NC) subjects were examined. The proportion of ICOS+ Tregs in the peripheral blood and intracellular cytokine levels in these cells were assessed using flow cytometry. The percentage of ICOS+ Tregs increased in the cohort of patients with RA compared with the NCs. Such increases were much larger in patients with inactive RA compared with patients with active RA. Additionally, ICOS+ Tregs expressed multiple suppressive cytokines, including interleukin (IL)-10, transforming growth factor-β and IL-35, but expressed low levels of IL-17. Importantly, the expression of suppressive cytokines in ICOS+ Tregs from patients with active RA decreased, but IL-17 expression noticeably increased compared with patients with inactive RA. The present findings suggested that ICOS+ Tregs may perform inflammatory and inhibitory functions, and abnormal ICOS+ Tregs numbers and functions may contribute to the pathogenesis of RA.
- Published
- 2018
48. A case report: autosomal recessive Myotonia congenita caused by a novel splice mutation (c.1401 + 1G A) in CLCN1 gene of a Chinese Han patient
- Author
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Xue-mei Liu, Zhi-xia Kang, Yan-lu Gao, Xiao-jing Wei, Xue-fan Yu, and Jing Miao
- Subjects
0301 basic medicine ,musculoskeletal diseases ,Male ,medicine.medical_specialty ,Genotype ,Myotonia Congenita ,Autosomal recessive ,Compound heterozygosity ,lcsh:RC346-429 ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Asian People ,Chloride Channels ,Internal medicine ,Mexiletine ,Case report ,Medicine ,Humans ,lcsh:Neurology. Diseases of the nervous system ,CLCN1 ,biology ,business.industry ,Myotonia congenita ,Muscle weakness ,General Medicine ,Muscle stiffness ,CLCN1 gene ,medicine.disease ,030104 developmental biology ,Endocrinology ,Mutation (genetic algorithm) ,Mutation ,biology.protein ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Muscle contraction ,medicine.drug - Abstract
Background Autosomal recessive Myotonia congenita (Becker’s disease) is caused by mutations in the CLCN1 gene. The condition is characterized by muscle stiffness during sustained muscle contraction and variable degree of muscle weakness that tends to improve with repeated contractions. Case presentation A 21-year-old man presented with transient muscle stiffness since the last 10 years. He had difficulty in initiating movement and experienced muscle weakness after rest, which typically improved after repeated contraction (warm-up phenomenon). There was no significant family history. Medical examination showed generalized muscle hypertrophy. Serum creatine kinase level was 2-fold higher than the normal value. Electromyogram showed myotonic discharges. DNA sequence analysis identified a novel splice mutation (c.1401 + 1G > A) and a known mutation (c.1657A > T,p.Ile553Phe). He rapidly responded to treatment with mexiletine 100 mg three times a day for 6 months. Conclusions This case report of autosomal recessive Myotonia congenita caused by a novel compound heterozygous mutation expands the genotypic spectrum of CLCN1 gene.
- Published
- 2018
49. Egr-1 increases angiogenesis in cartilage via binding Netrin-1 receptor DCC promoter
- Author
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Wei Zheng, Ying Chen, Kai Jiang, Da Liu, Xia Kang, and Jun Sheng
- Subjects
Egr-1 ,0301 basic medicine ,lcsh:Diseases of the musculoskeletal system ,Angiogenesis ,DDC promoter ,Netrin-1 receptor ,Osteoarthritis ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Netrin ,Animals ,Medicine ,Orthopedics and Sports Medicine ,Electrophoretic mobility shift assay ,Promoter Regions, Genetic ,Early Growth Response Protein 1 ,030203 arthritis & rheumatology ,business.industry ,Cartilage ,fungi ,DCC Receptor ,medicine.disease ,Rats ,Vascular endothelial growth factor ,lcsh:RD701-811 ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Rats, Inbred Lew ,Cancer research ,Angiogenesis Inducing Agents ,Surgery ,lcsh:RC925-935 ,Netrin Receptors ,business ,Protein Binding ,Research Article - Abstract
Background Osteoarthritis (OA) is a joint disease characterized by degradation of cartilage. The etiology of OA is still unclear. Vascular endothelial growth factor (VEGF) plays a key role of angiogenesis in the pathogenesis of OA and contributes to the angiogenesis of NT-1/DCC. Whether or not NT-1/DCC and VEGF interact in regulating angiogenesis of OA cartilage is not known. Methods Histological studies for CD34, VEGF, and safranin-O staining were performed to determine angiogenesis and cartilage tissue injury. ELISA indicated the level of pro-inflammation cytokines. Immunoblotting, immunoprecipitation, and electrophoretic mobility shift assay (EMSA) were performed to assay the expression and function of NT-1/DCC-VEGF signaling pathway. Results Our data indicated that VEGF expression was increased in cartilage tissue from OA rats, while the chondrocytes were disorganized, and cartilage degeneration was increasing in OA rats. The inflammation factors in articular cavity fluid were higher in the OA rats than in the sham. The protein expression of NT-1, DCC, and VEGF were increased in osteoarthritic cartilage. DCC was involved in the positive regulation of osteoarthritic angiogenesis by VEGF. Egr-1 expression was higher in OA rats than in sham rats. Egr-1 is a regulator of DCC promoter activity, and the binding is higher in OA rats than in sham rats. Conclusion Our present study provides a mechanism by which Egr-1 induced angiogenesis via NT-1/DCC-VEGF pathway.
- Published
- 2018
50. Acceptability of physical examination by male doctors in medical care: Taking breast palpation as an example
- Author
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Ping Zhang, Zhanchun Feng, Li-xia Kang, Yan-jie Wang, Dong-ming Chen, Jie Yang, and Zhen Jia
- Subjects
Adult ,Male ,Students, Medical ,Health Personnel ,media_common.quotation_subject ,Population ,Biomedical Engineering ,Physical examination ,Biochemistry ,Palpation ,Biomaterials ,Asian People ,Nursing ,Physicians ,Surveys and Questionnaires ,Outpatients ,Genetics ,medicine ,Humans ,Mammary Glands, Human ,education ,Physical Examination ,Earth-Surface Processes ,media_common ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Middle Aged ,Patient Acceptance of Health Care ,Test (assessment) ,Feeling ,Anxiety ,Female ,Health education ,medicine.symptom ,business ,Medical ethics - Abstract
In this study, we conducted an investigation among medical workers, patients and college students concerning their acceptability of breast palpation performed by male doctors (hereinafter referred to as "acceptability", or "the examination", respectively, if not otherwise indicated), to get the information about their acceptability and reasons for accepting or declining the examination among the three population. A questionnaire investigation was conducted in 500 patients with breast diseases, 700 students of medical colleges, and 280 medical workers working in hospitals. The subjects were asked to choose between two options: accept or do not accept (the examination). The subjects were asked to fill out the questionnaire forms on free and anonymous basis and the questionnaire forms were collected on spot, immediately after completion. The questionnaires collected were coded, sorted out and checked. Data of the eligible questionnaires were input into Epidata software and analyzed by SPSS. Upon the establishment of the database, the intra-group data were tested by utilizing χ(2) test. Among 1480 questionnaires, 1293 (90.41%) questionnaires were retrieved. Our results showed that 56.78% of patients reported that they could accept breast palpation by male doctors. About 59.66% of medical staff expressed their acceptance of the examination, but only 35.03% of students said the examination. On the basis of this study, we were led to conclude that the examination is not well accepted by different populations, and therefore, (1) medical professionals and administrators should pay attention to the gender-related ethics in their practice and the feeling of patients should be respected when medical examinations involve private or sensitive body parts; (2) to this end, related departments should be properly staffed with doctors of both sexes, and this is especially true of the departments involving the examination or treatment of private or sensitive body parts; (3) health education should, among other things, include helping female patients to overcome the fear and anxiety in such examinations. This is of great importance since some women may miss the opportunity to get timely diagnosis.
- Published
- 2015
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