1. Thermal cycling-hyperthermia in combination with polyphenols, epigallocatechin gallate and chlorogenic acid, exerts synergistic anticancer effect against human pancreatic cancer PANC-1 cells
- Author
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Yu-Yi Kuo, Chih-Yu Chao, Wei-Ting Chen, Chih-Hsiung Hsieh, and Chueh-Hsuan Lu
- Subjects
0301 basic medicine ,Cytotoxicity ,Cancer Treatment ,Apoptosis ,Epigallocatechin gallate ,Pharmacology ,Toxicology ,Pathology and Laboratory Medicine ,Biochemistry ,Catechin ,chemistry.chemical_compound ,Spectrum Analysis Techniques ,0302 clinical medicine ,Medicine and Health Sciences ,Cell Cycle and Cell Division ,Energy-Producing Organelles ,Staining ,Multidisciplinary ,Cell Death ,Cell Staining ,Drug Synergism ,Cell cycle ,Flow Cytometry ,Combined Modality Therapy ,Mitochondria ,Oncology ,Cell Processes ,Spectrophotometry ,030220 oncology & carcinogenesis ,Medicine ,Cytophotometry ,Cellular Structures and Organelles ,Chlorogenic Acid ,Research Article ,Hyperthermia ,Side effect ,Science ,Antineoplastic Agents ,Bioenergetics ,Research and Analysis Methods ,Pancreatic Cancer ,03 medical and health sciences ,Cell Line, Tumor ,Pancreatic cancer ,Gastrointestinal Tumors ,medicine ,Humans ,Biology and Life Sciences ,Cancers and Neoplasms ,Polyphenols ,Cell Biology ,Cell Cycle Checkpoints ,Hyperthermia, Induced ,medicine.disease ,Pancreatic Neoplasms ,030104 developmental biology ,chemistry ,Specimen Preparation and Treatment ,Cell culture ,Cancer cell - Abstract
Hyperthermia (HT) has shown feasibility and potency as an anticancer therapy. Administration of HT in the chemotherapy has previously enhanced the cytotoxicity of drugs against pancreatic cancer. However, the drugs used when conducting these studies are substantially conventional chemotherapeutic agents that may cause unwanted side effects. Additionally, the thermal dosage in the treatment of cancer cells could also probably harm the healthy cells. The purpose of this work was to investigate the potential of the two natural polyphenolic compounds, epigallocatechin gallate (EGCG) and chlorogenic acid (CGA), as heat synergizers in the thermal treatment of the PANC-1 cells. Furthermore, we have introduced a novel strategy entitled the thermal cycling-hyperthermia (TC-HT) that is capable of providing a maximum synergy and minimal side effect with the anticancer compounds. Our results demonstrate that the combination of the TC-HT and the CGA or EGCG markedly exerts the anticancer effect against the PANC-1 cells, while none of the single treatment induced such changes. The synergistic activity was attributed to the cell cycle arrest at the G2/M phase and the induction of the ROS-dependent mitochondria-mediated apoptosis. These findings not only represent the first thermal synergistic study of natural compounds in the treatment of pancreatic cancer, but also highlight the potential application of the TC-HT as an alternative strategy in anticancer treatment.
- Published
- 2019