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The sodium-iodide symporter (NIS) is primarily a thyroid protein, providing for the accumulation of iodide for biosynthesis of thyroid hormones. Native NIS expression has made possible the use of radioactive iodide to image and treat thyroid disease successfully. The current study, using adult male beagle dogs, was carried out in preparation for a Phase I clinical trial of adenovirus-mediated NIS gene (approved symbol SLC5A5) therapy for prostate cancer. Direct intraprostatic injection of virus (Ad5/CMV/NS) was followed by iv injection of 3 mCi 123I and serial image acquisition. The dogs were then given a therapeutic dose of 131I (116 mCi/m2) and observed for 7 days. SPECT/CT fusion imaging revealed clear images of the NIS-transduced prostates. Dosimetry calculations revealed an average absorbed dose to the prostate of 23 +/- 42 cGy/mCi 131I, with acceptably low radiation doses to other organs. This study demonstrated the successful introduction of localized NIS expression in the prostate gland of dogs, with no vector-related toxicity observed. None of the animals experienced any surgical complications, and serum chemistry panels showed no significant change following therapy. The results presented provide further evidence of the safety and efficacy of NIS as a therapeutic gene and support translation of this work into the clinical setting.

Wet-tail of golden hamsters (Mesocricetus auratus) has been referred to as proliferative ileitis, regional enteritis, terminal ileitis, enzootic intestinal adenocarcinoma, atypical ileal hyperplasia, and hamster enteritis. The disease will be referred to as hamster enteritis (HE) in this review. Hamster enteritis is characterized clinically by diarrhoea in weanlings, and pathologically by enteritis, sometimes proliferative, involving primarily the small intestine. Trum & Routledge (1967) stated that 'wet-tailor terminal ileitis is the only recognizable common disease syndrome in the hamster'. Although this was an exaggeration because other common diseases have been well described, HE is still the most common spon