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Cell nucleus is a body including various components and it has complicated and heterogeneous structure. Though most functions of nuclear domains have been well studied, mechanical properties of the nuclear domains has not been studied. In this study, the experimental system was developed to measure the stiffness of living cell nucleus in order to verify the inhomogeneity of the cell nucleus. The system is composed of two main devices: a sensor which can measure stiffness of micro cell nucleus and a rotatable device which can rotate nucleus horizontally. The devices are assembled on the stage of an inverted phase contrast microscope. Experimental studies have been carried out by using normal human osteoblast. The method has shown capability to detect difference of stiffness on cell nucleus. There is significant difference between nucleolus and other nuclear domains.