8 results on '"Cai-Ping Ren"'
Search Results
2. The clinicopathological significance of miR-149 and PARP-2 in hepatocellular carcinoma and their roles in chemo/radiotherapy
- Author
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Yuxiang Chen, Yangde Zhang, Ling Lin, Zi-Yu Chen, and Cai-Ping Ren
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Male ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,medicine.medical_treatment ,Blotting, Western ,Mice, Nude ,Pathogenesis ,03 medical and health sciences ,Western blot ,Internal medicine ,medicine ,Animals ,Humans ,neoplasms ,Survival rate ,Survival analysis ,Mice, Inbred BALB C ,Chemotherapy ,medicine.diagnostic_test ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Liver Neoplasms ,Chemoradiotherapy ,Hep G2 Cells ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Immunohistochemistry ,Survival Analysis ,Xenograft Model Antitumor Assays ,digestive system diseases ,Tumor Burden ,Radiation therapy ,MicroRNAs ,Treatment Outcome ,030104 developmental biology ,Liver ,Hepatocellular carcinoma ,Female ,RNA Interference ,Poly(ADP-ribose) Polymerases ,business - Abstract
Hepatocellular carcinomas (HCC) are commonly diagnosed at an advanced stage with unresectable tumors. Although numerous non-surgical approaches have been developed to treat HCC, the prognosis of patients with HCC is still poor. This study investigated the expression of miR-149 and PARP-2 in HCC tumor tissues and their roles in sensitizing chemo/radiotherapy. The expression of miR-149 was measured by real-time PCR, and PARP-2 protein was measured by immunohistochemistry and Western blot. The xenograft HCC mouse model was established by inoculating Hep G2 cells. Increased PARP-1 and decreased miR-149 expression was observed in HCC tissues compared to peritumoral tissues. Positive PARP-2 and low miR-149 expression correlated with larger tumor mass size (P
- Published
- 2016
3. Roles of flotillins in tumors
- Author
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Xiao Shi, Weidong Liu, Lei Wang, Bin Zhu, Mu Sheng Zeng, Cai ping Ren, Zi xuan Peng, Mei zuo Zhong, Xing dong Liu, Dan Xie, Xu xu Liu, and He cheng Zhu
- Subjects
0301 basic medicine ,Cellular differentiation ,medicine.medical_treatment ,Cell ,Review ,Biology ,Retinal ganglion ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Targeted therapy ,03 medical and health sciences ,0302 clinical medicine ,Neoplasms ,medicine ,Animals ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,General Veterinary ,Regeneration (biology) ,Membrane Proteins ,Cell Differentiation ,General Medicine ,medicine.disease ,Endocytosis ,Nerve Regeneration ,030104 developmental biology ,medicine.anatomical_structure ,Membrane protein ,030220 oncology & carcinogenesis ,Cancer research ,Signal transduction - Abstract
The identification and use of molecular biomarkers have greatly improved the diagnosis and treatment of malignant tumors. However, a much deeper understanding of oncogenic proteins is needed for the benefit to cancer patients. The lipid raft marker proteins, flotillin-1 and flotillin-2, were first found in goldfish retinal ganglion cells during axon regeneration. They have since been found in a variety of cells, mainly on the inner surface of cell membranes, and not only act as a skeleton to provide a platform for protein-protein interactions, but also are involved in signal transduction, nerve regeneration, endocytosis, and lymphocyte activation. Previous studies have shown that flotillins are closely associated with tumor development, invasion, and metastasis. In this article, we review the functions of flotillins in relevant cell processes, their underlying mechanisms of action in a variety of tumors, and their potential applications to tumor molecular diagnosis and targeted therapy.
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- 2018
4. FMNL1 mediates nasopharyngeal carcinoma cell aggressiveness by epigenetically upregulating MTA1
- Author
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Mu Yan Cai, Feng Wei Wang, Lin Quan Tang, Dan Xie, Wen Hui Chen, Chao-Nan Qian, Mu Sheng Zeng, Cai ping Ren, Jia Xing Zhang, Hai-Qiang Mai, Yiguo Jiang, Ling Guo, Hsiang-Fu Kung, Xiao Han Jin, Chen Yuan Wang, and Yi Ji Liao
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Cytoplasm ,Epithelial-Mesenchymal Transition ,Cell ,Formins ,Mice, Nude ,Histone Deacetylase 1 ,Mice, SCID ,Biology ,Histone Deacetylases ,Epigenesis, Genetic ,03 medical and health sciences ,Mice ,Profilins ,0302 clinical medicine ,Downregulation and upregulation ,Cell Line, Tumor ,otorhinolaryngologic diseases ,Genetics ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Promoter Regions, Genetic ,Molecular Biology ,Regulation of gene expression ,Mice, Inbred BALB C ,Nasopharyngeal Carcinoma ,Nasopharyngeal Neoplasms ,medicine.disease ,HDAC1 ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Repressor Proteins ,stomatognathic diseases ,Haematopoiesis ,Cytoskeletal Proteins ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Nasopharyngeal carcinoma ,Cell culture ,030220 oncology & carcinogenesis ,Invadopodia ,Cancer research ,Trans-Activators - Abstract
It has been suggested that formin-like protein 1 (FMNL1) plays an important role in the pathogenic process of several hematopoietic malignancies. In this study, we performed a series of in vivo and in vitro assays to elucidate the biological functions of FMNL1 and underlying mechanisms in human nasopharyngeal carcinoma (NPC) pathogenesis. Herein, we report that high expression of FMNL1 in NPC is positively associated with an aggressive disease and/or poor patient survival. Ectopic overexpression of FMNL1 in NPC cells substantially promoted cell invadopodia formation, epithelial-mesenchymal transition (EMT) and invasiveness, whereas depletion of FMNL1 potently suppressed NPC cells invadopodia formation, EMT, and invasive/metastatic capacities. We further show that FMNL1 could enhance NPC cell aggressiveness by increasing a key downstream target, the metastasis-associated protein 1 (MTA1) gene. Importantly, ectopic overexpression of FMNL1 in NPC cells markedly improved the binding of HDAC1 with Profilin2 in the cytoplasm and suppressed the enrichment of HDAC1 on the promoter of MTA1 and thereby, leading to an increased MTA1 transcription and expression. Furthermore, in addition to the amplification of FMNL1 gene, decreased level of miR-16 in NPCs is another critical mechanism to upregulate FMNL1 expression. These results, collectively, provide first-line of evidences that high expression of FMNL1, resulted from decreased miR-16 and/or MTA1 amplification, has a potent oncogenic role to drive the development and aggressive process of NPC by upregulating MTA1, and FMNL1 might be employed as a new prognostic biomarker and therapeutic target for human NPC.
- Published
- 2017
5. Preparation of human scFv antibody against nasopharyngeal carcinoma and identification of its specificity
- Author
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Yan-Dong Li, Ping-Li Xie, Jiajia Wang, Yan Zhao, Yuehui Li, Guancheng Li, Fengjie Guo, and Cai-Ping Ren
- Subjects
Antibodies, Neoplasm ,Mice, Nude ,chemical and pharmacologic phenomena ,Enzyme-Linked Immunosorbent Assay ,Biology ,Virus ,Green fluorescent protein ,Mice ,In vivo ,Cell Line, Tumor ,Immunochemistry ,otorhinolaryngologic diseases ,medicine ,Animals ,Humans ,Nasopharyngeal Carcinoma ,Carcinoma ,Nasopharyngeal Neoplasms ,General Medicine ,Neoplasms, Experimental ,respiratory system ,medicine.disease ,Fusion protein ,Virology ,Molecular biology ,Immunohistochemistry ,In vitro ,stomatognathic diseases ,Otorhinolaryngology ,Nasopharyngeal carcinoma ,biology.protein ,Female ,Antibody ,Single-Chain Antibodies - Abstract
Conclusion: The selected scFv antibody could specifically recognize and target nasopharyngeal carcinoma (NPC), and could be applied to clinical diagnosis and therapy. Objective: The aim was to construct and screen fully human anti-NPC single chain Fv fusion phage libraries, and to identify the specificity of the scFv antibody. Methods: Peripheral blood mononuclear cells of patients with NPC were immunized in vitro by NPC cells and transformed by Epstein-Barr virus. The total RNAwas used to construct the scFv libraries. By means of ELISA and immunochemistry, the positively bound scFv was selected and identified. The positive scFv was fused to EGFP, and was then expressed in E. coli strain BL21 (DE3) and purified. Furthermore, we observed the binding bioactivity. Results: The fusion protein has the biological activity of binding the NPC cells and emitting green fluorescence. In targeting experiments in vivo, the results showed that the fusion protein can successfully target the NPC.
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- 2012
6. The DLC-1 -29A/T polymorphism is not associated with nasopharyngeal carcinoma risk in Chinese population
- Author
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Yi-Bo Zhou, Hong Li, Kaitai Yao, Cai-Ping Ren, Weiyi Fang, Wen Zhou, and Xiangling Feng
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Adult ,Male ,China ,Adolescent ,DNA Mutational Analysis ,Gene mutation ,Biology ,Polymorphism, Single Nucleotide ,Young Adult ,Asian People ,Gene Frequency ,Population Groups ,Risk Factors ,otorhinolaryngologic diseases ,medicine ,SNP ,Humans ,Allele ,Genetics (clinical) ,Alleles ,Polymorphism, Single-Stranded Conformational ,Aged ,Genetics ,Aged, 80 and over ,Polymorphism, Genetic ,Tumor Suppressor Proteins ,Carcinoma ,GTPase-Activating Proteins ,Promoter ,Nasopharyngeal Neoplasms ,Middle Aged ,medicine.disease ,Candidate Tumor Suppressor Gene ,Genotype frequency ,stomatognathic diseases ,Nasopharyngeal carcinoma ,Case-Control Studies ,Female ,SNP array - Abstract
Deleted in liver cancer-1 (DLC-1), encoding a Rho GTPase-activating protein (GAP), is considered as a promising candidate tumor suppressor gene in nasopharyngeal carcinoma (NPC). The single-nucleotide polymorphism (SNP) −29A/T upstream of ATG start codon was found when gene mutation profile of DLC-1 in NPC was analyzed. To evaluate the correlation between SNP −29A/T in the promoter region of DLC-1 gene and risk of NPC, a total of 521 samples from a Chinese population, including 320 healthy individuals and 201 NPC patients, were collected for SNP analysis by PCR–single-strand conformation polymorphism and sequencing. The differences in allele and genotype frequencies between NPC patients and controls were tested using logistic regression statistical method. No significant differences were found in allele or genotype frequencies between NPC patients and controls or among different NPC clinical stages. Hence, our data indicate that the SNP −29A/T of DLC-1 gene is not associated with NPC susceptibility.
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- 2008
7. An imageable metastatic treatment model of nasopharyngeal carcinoma
- Author
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Robert M. Hoffman, Shuang Wang, Kaitai Yao, Weiyi Fang, Yanqin Ding, Xiaoyan Bai, Tengfei Liu, Xin Li, Zu-Guo Li, Cai-Ping Ren, and Weibing Xie
- Subjects
Models, Anatomic ,Cancer Research ,Pathology ,medicine.medical_specialty ,Angiogenesis ,Green Fluorescent Proteins ,Nasopharyngeal neoplasm ,Mice, Nude ,Antineoplastic Agents ,Metastasis ,Mice ,Cell Line, Tumor ,Carcinoma ,medicine ,Animals ,Humans ,Neoplasm Metastasis ,Neovascularization, Pathologic ,business.industry ,Micrometastasis ,Nasopharyngeal Neoplasms ,medicine.disease ,Primary tumor ,Disease Models, Animal ,Oncology ,Nasopharyngeal carcinoma ,Microscopy, Fluorescence ,Cancer cell ,business ,Neoplasm Transplantation - Abstract
Purpose: Nasopharyngeal carcinoma is highly prevalent in southern China and is often resistant to current treatment options.Experimental Design: Clinically relevant mouse models are necessary for further understanding and drug discovery in this disease. Two nasopharyngeal carcinoma cell lines, stably expressing green fluorescent protein (GFP), 5-8F-GFP and 6-10B-GFP, were established. The cells were orthotopically injected into the nasopharynx or ectopically into the subcutis of nude mice. Whole-body fluorescence imaging was used to monitor the growth of the primary tumor as well as angiogenesis and metastasis.Results: The metastatic behavior of 5-8F and 6-10B were distinct in the orthotopic model. Orthotopic implantation of highly metastatic 5-8F cells resulted in brain invasion, cervical lymph node metastases, and pulmonary metastases similar to what is often observed in patients. Cell line 6-10B was less metastatic, which occasionally resulted in pulmonary metastasis. GFP enabled imaging of micrometastasis. Neither 5-8F nor 6-10B were metastatic in the s.c. site. These results indicated that, in addition to the cancer cell type, the host microenvironment was critical for metastasis to occur consistent with the “seed-and-soil” hypothesis. 5-8F was highly sensitive to 5-fluorouracil (5-FU), whereas 6-10B was moderately sensitive.Conclusions: The imageable orthotopic model should play a critical role in elucidating the mechanisms involved in the growth, progression, metastasis, and angiogenesis of nasopharyngeal carcinoma and for evaluation of novel compounds with potential efficacy.
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- 2007
8. Reexploring the possible roles of some genes associated with nasopharyngeal carcinoma using microarray-based detection
- Author
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Kai tai Yao, Yan Qing Ding, Zhong xi Huang, Qiu Zhen Liu, Cai Ping Ren, Xin Li, Wei Yi Fang, Xin Deng, Wei Bing Xie, Teng-Fei Liu, Xu Yu Yang, and Shuang Wang
- Subjects
Cell type ,Microarray ,Biophysics ,Nasopharyngeal neoplasm ,Cathepsin D ,Biology ,Biochemistry ,otorhinolaryngologic diseases ,medicine ,Biomarkers, Tumor ,Tumor Cells, Cultured ,Humans ,Genetic Predisposition to Disease ,Genetic Testing ,Gene ,Oligonucleotide Array Sequence Analysis ,Gene Expression Profiling ,Carcinoma ,Nasopharyngeal Neoplasms ,General Medicine ,medicine.disease ,Neoplasm Proteins ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,Nasopharyngeal carcinoma ,Cancer research ,DNA microarray - Abstract
In gene expression profiling, nasopharyngeal carcinoma (NPC) 5-8F cells differ from 6-10B cells in terms of their high tumorigenicity and metastatic ability. Differentially expressed genes from the two cell types were analyzed by combining with MILANO (the automatic custom annotation of microarray results which is based on all the available published work in PubMed). The results showed that five genes, including CTSD, P63, CSE1L, BPAG1 and EGR1, have been studied or mentioned in published work on NPC. Subsequently, we reevaluated the roles of these genes in the pathogenesis of NPC by combining the data of gene chips from NPCs versus NPs and pooled cells from 5-8F, 6-10B and CNE2 versus NPs. The results suggested that the roles of BPAG1 and EGR1 are possibly different from those reported in previous NPC studies. These five genes are likely to be involved in the proliferation, apoptosis, invasion and metastasis of NPC. A reexploration of the genes will further define their roles in the pathogenesis of NPC.
- Published
- 2005
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