1. Evaluation of small intestinal damage in a rat model of 6 Minutes cardiac arrest
- Author
-
Thorsten Annecke, Alexandra C. Maul, Xiaowei Yan, Esther Mahabir, Maria Guschlbauer, Bernd W. Böttiger, Tim Hucho, Insa Bultmann-Mellin, Daniel C. Schroeder, Anja Sterner-Kock, Holger Herff, Isabell Koxholt, and Stephan A. Padosch
- Subjects
Male ,medicine.medical_specialty ,Resuscitation ,Time Factors ,medicine.medical_treatment ,030204 cardiovascular system & hematology ,Return of spontaneous circulation ,Systemic inflammation ,Jejunum ,lcsh:RD78.3-87.3 ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Ischaemia-reperfusion-injury ,Internal medicine ,Intestine, Small ,medicine ,Animals ,Intestinal Mucosa ,Rats, Wistar ,business.industry ,030208 emergency & critical care medicine ,Small intestine ,medicine.disease ,Cardiac arrest ,Heart Arrest ,Rats ,Systemic inflammatory response syndrome ,Disease Models, Animal ,Anesthesiology and Pain Medicine ,Endocrinology ,Cytokine ,medicine.anatomical_structure ,lcsh:Anesthesiology ,Reperfusion Injury ,Cytokines ,medicine.symptom ,business ,Research Article - Abstract
Background Contribution of the small intestine to systemic inflammation after cardiac arrest (CA) is poorly understood. The objective was to evaluate whether an in vivo rat model of 6 min CA is suitable to initiate intestinal ischaemia-reperfusion-injury and to evaluate histomorphological changes and inflammatory processes in the small intestinal mucosa resp. in sera. Methods Adult male Wistar rats were subjected to CA followed by cardio-pulmonary resuscitation. Proximal jejunum and serum was collected at 6 h, 24 h, 72 h and 7 d post return of spontaneous circulation (ROSC) and from a control group. The small intestine was evaluated histomorphologically. Cytokine concentrations were measured in jejunum lysates and sera. Results Histomorphological evaluation revealed a significant increase in mucosal damage in the jejunum at all timepoints compared to controls (p
- Published
- 2018