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Your search keyword '"Isabelle Fleurot"' showing total 3 results
Start Over Author "Isabelle Fleurot" Topic medicine.disease
3 results on '"Isabelle Fleurot"'

1. Evidence of early increased sialylation of airway mucins and defective mucociliary clearance in CFTR-deficient piglets

Author

Catherine Robbe-Masselot, Mustapha Si-Tahar, Ignacio S. Caballero, Renaud Léonard, Nicolas Pons, Andrea Bähr, Pascal Barbry, Antoine Guillon, Agnès Paquet, Claire Chevaleyre, Nikolai Klymiuk, Isabelle Fleurot, Mustapha Berri, Bélinda Ringot-Destrez, Kevin Lebrigand, Carole Baron, Céline Barc, Reuben Ramphal, Isabelle Lantier, Centre National de la Recherche Scientifique (CNRS), Université Côte d'Azur (UCA), Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Université Côte d'Azur (UCA), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Service de Médecine Intensive et Réanimation [Tours], Plateforme d'Infectiologie Expérimentale (PFIE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ludwig Maximilian University [Munich] (LMU), Association Vaincre la Mucoviscidose (Grant No. RF20150 501357), Association Grégory Lemarchal (Grant No. RIF20160501690), Fondation pour la Recherche Médicale (DEQ20180339158), ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Chanteloup, Nathalie Katy, Centres d'excellences - Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie - - SIGNALIFE2011 - ANR-11-LABX-0028 - LABX - VALID, Organisation et montée en puissance d'une Infrastructure Nationale de Génomique - - France-Génomique2010 - ANR-10-INBS-0009 - INBS - VALID, Université de Tours (UT), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT), Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Gene Center and Center for Innovation Medical Models (CiMM), Ludwig Maximilians University of Munich, Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)

Subjects
0301 basic medicine, Pulmonary and Respiratory Medicine, Male, Glycosylation, Mucociliary clearance, Swine, [SDV]Life Sciences [q-bio], Sus scrofa, Cystic Fibrosis Transmembrane Conductance Regulator, Context (language use), Inflammation, Respiratory Mucosa, Mucociliary transport, medicine.disease_cause, Cystic fibrosis, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Medicine, Animals, CFTR, ComputingMilieux_MISCELLANEOUS, Lung, medicine.diagnostic_test, business.industry, Pseudomonas aeruginosa, Mucin, Mucins, respiratory system, medicine.disease, 3. Good health, respiratory tract diseases, Trachea, [SDV] Life Sciences [q-bio], Mucin glycosylation, 030104 developmental biology, medicine.anatomical_structure, Bronchoalveolar lavage, 030228 respiratory system, Animals, Newborn, Mucociliary Clearance, Pediatrics, Perinatology and Child Health, Female, medicine.symptom, MESH: CFTR, business
Abstract

International audience; Background: Bacterial colonization in cystic fibrosis (CF) lungs has been directly associated to the loss of CFTR function, and/or secondarily linked to repetitive cycles of chronic inflammation/infection. We hypothesized that altered molecular properties of mucins could contribute to this process.Methods: Newborn CFTR+/+ and CFTR-/- were sacrificed before and 6 h after inoculation with luminescent Pseudomonas aeruginosa into the tracheal carina. Tracheal mucosa and the bronchoalveolar lavage (BAL) fluid were collected to determine the level of mucin O-glycosylation, bacteria binding to mucins and the airways transcriptome. Disturbances in mucociliary transport were determined by ex-vivo imaging of luminescent Pseudomonas aeruginosa.Results: We provide evidence of an increased sialylation of CF airway mucins and impaired mucociliary transport that occur before the onset of inflammation. Hypersialylation of mucins was reproduced on tracheal explants from non CF animals treated with GlyH101, an inhibitor of CFTR channel activity, indicating a causal relationship between the absence of CFTR expression and the sialylation of mucins. This increased sialylation was correlated to an increased adherence of P. aeruginosa to mucins. In vivo infection of newborn CF piglets by live luminescent P. aeruginosa demonstrated an impairment of mucociliary transport of this bacterium, with no evidence of pre-existing inflammation.Conclusions: Our results document for the first time in a well-defined CF animal model modifications that affect the O-glycan chains of mucins. These alterations precede infection and inflammation of airway tissues, and provide a favorable context for microbial development in CF lung that hallmarks this disease.

Published
2020
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2. Intrinsic alterations in peripheral neutrophils from cystic fibrosis newborn piglets

Author

Mustapha Berri, Isabelle Fleurot, Sandrine Melo, Laura Soler, Ignacio S. Caballero, Ana-Paula Teixeira-Gomes, Valérie Labas, Nicolas Cenac, Mustapha Si-Tahar, Julien Pujo, Andrea Bähr, Déborah Bréa, Claire Chevaleyre, Nikolai Klymiuk, Antoine Guillon, SEGUIN, Nathalie, Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'Analyses des Interactions Moléculaires, Plate-forme d'Analyses intégrative des Biomarqueurs cellulaires et moléculaires (PAIB), UMR6175, Institut National de la Recherche Agronomique (INRA), Institut de Recherche en Santé Digestive (IRSD ), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Gene Center and Center for Innovation Medical Models (CiMM), Ludwig-Maximilians University [Munich] (LMU), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Vaincre la Mucoviscidose [grant numbers RF20130500925 and RF2015050357, Region Centre (France) [grant number 320 0 0604 ]., Metatoul- Lipidomique Core Facility-MetaboHub, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours, Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ludwig Maximilians University of Munich, Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0301 basic medicine, Pulmonary and Respiratory Medicine, Male, Proteomics, Swine, Neutrophils, [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], [SDV]Life Sciences [q-bio], Inflammation, medicine.disease_cause, Cystic fibrosis, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Immunophenotyping, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Animals, CFTR, chemistry.chemical_classification, Reactive oxygen species, Proteomic Profile, Lung, medicine.diagnostic_test, Pseudomonas aeruginosa, business.industry, Models, Theoretical, medicine.disease, Pig model, Molecular biology, 3. Good health, [SDV] Life Sciences [q-bio], Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, 030228 respiratory system, chemistry, Animals, Newborn, ICM-MS, Pediatrics, Perinatology and Child Health, Fatty Acids, Unsaturated, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, medicine.symptom, business, Reactive Oxygen Species
Abstract

International audience; Background: The hallmark of the cystic fibrosis (CF) lung disease is a neutrophil dominated lung environment that is associated to chronic lung tissue destruction and ultimately the patient's death. It is unclear whether the exacerbated neutrophil response is primary related to a defective CFTR or rather secondary to chronic bacterial colonization and inflammation. Here, we hypothesized that CF peripheral blood neutrophils present intrinsic alteration at birth before the start of an inflammatory process.Methods: Peripheral blood neutrophils were isolated from newborn CFTR+/+ and CFTR-/- piglets. Neutrophils immunophenotype was evaluated by flow cytometry. Lipidomic and proteomic profile were characterized by liquid chromatography/tandem mass spectrometry (LC-MS/MS), intact cell matrix-assisted laser desorption/ionization mass spectrometry (ICM-MS) followed by top-down high-resolution mass spectrometry (HRMS), respectively. The ability of CF neutrophils to kill pseudomonas aeruginosa was also evaluated.Results: Polyunsaturated fatty acid metabolites analysis did not show any difference between CFTR+/+ and CFTR-/- neutrophils. On the other hand, a predictive mathematical model based on the ICM-MS proteomic profile was able to discriminate between both genotypes. Top-down proteomic analysis identified 19 m/z differentially abundant masses that corresponded mainly to proteins related to the antimicrobial response and the generation of reactive oxygen species (ROS). However, no alteration in the ability of CFTR-/- neutrophils to kill pseudomonas aeruginosa in vitro was observed.Conclusions: ICM-MS demonstrated that CFTR-/- neutrophils present intrinsic alterations already at birth, before the presence of any infection or inflammation.

Published
2020
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3. Following Pathogen Development and Gene Expression in a Food Ecosystem: the Case of a Staphylococcus aureus Isolate in Cheese

Author

Jacques-Antoine Hennekinne, Alexandra Gruss, Renaud Fleurot, Elise Borezée-Durant, Claire Darrigo, Marina Aigle, A. Delacroix-Buchet, Isabelle Fleurot, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), Unité Staphylocoques, Bacillus, Clostridies, Lait (Unité SBCL), Laboratoire de sécurité des aliments de Maisons-Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Ile-de-France, Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl), and Fleurot, Isabelle

Subjects
Staphylococcus aureus, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], milk, bacteria, United-States, identification, dairy-product, in-situ hybridization, microstructure, foodborne illness, enterotoxin production, lactococcus-lactis, Ingénierie des aliments, Food Contamination, medicine.disease_cause, Staphylococcal infections, Applied Microbiology and Biotechnology, Microbiology, Enterotoxins, Bacterial Proteins, Cheese, [SDV.IDA]Life Sciences [q-bio]/Food engineering, medicine, Food microbiology, Food engineering, Humans, Pathogen, Ecosystem, 2. Zero hunger, Food poisoning, Ecology, biology, Lactococcus lactis, Staphylococcal Infections, medicine.disease, biology.organism_classification, Food Microbiology, Bacteria, Food Science, Biotechnology, Food contaminant, Autre (Sciences du Vivant)
Abstract

Human intoxication or infection due to bacterial food contamination constitutes an economic challenge and a public health problem. Information on the in situ distribution and expression of pathogens responsible for this risk is to date lacking, largely because of technical bottlenecks in detecting signals from minority bacterial populations within a complex microbial and physicochemical ecosystem. We simulated the contamination of a real high-risk cheese with a natural food isolate of Staphylococcus aureus , an enterotoxin-producing pathogen responsible for food poisoning. To overcome the problem of a detection limit in a solid matrix, we chose to work with a fluorescent reporter (superfolder green fluorescent protein) that would allow spatiotemporal monitoring of S. aureus populations and targeted gene expression. The combination of complementary techniques revealed that S. aureus localizes preferentially on the cheese surface during ripening. Immunochemistry and confocal laser scanning microscopy enabled us to visualize, in a single image, dairy bacteria and pathogen populations, virulence gene expression, and the toxin produced. This procedure is readily applicable to other genes of interest, other bacteria, and different types of food matrices.

Published
2014
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