1. Construction of CNA35 Collagen-Targeted Phase-Changeable Nanoagents for Low-Intensity Focused Ultrasound-Triggered Ultrasound Molecular Imaging of Myocardial Fibrosis in Rabbits
- Author
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Qingsong Xiong, Qin Zhou, Chris P. M. Reutelingsperger, Frits W. Prinze, Yuehui Yin, Haitao Ran, Pan Li, Yalin Zeng, Zhiyu Ling, Shigen Zhong, RS: CARIM - R1 - Thrombosis and haemostasis, Biochemie, RS: CARIM - R2 - Cardiac function and failure, Fysiologie, RS: Carim - B02 Vascular aspects thrombosis and Haemostasis, and RS: Carim - H06 Electro mechanics more...
- Subjects
Materials science ,diagnosis ,Cardiac fibrosis ,02 engineering and technology ,010402 general chemistry ,THERAPY ,01 natural sciences ,NANODROPLETS ,Nanocomposites ,ENHANCEMENT ,ultrasound molecular imaging ,MAGNETIC-RESONANCE ,medicine ,Animals ,Humans ,General Materials Science ,Myocardial infarction ,Ultrasonography ,medicine.diagnostic_test ,business.industry ,Myocardium ,Ultrasound ,Magnetic resonance imaging ,Heart ,021001 nanoscience & nanotechnology ,medicine.disease ,CNA35 ,nanomedicine ,MICROBUBBLES ,Fibrosis ,0104 chemical sciences ,Disease Models, Animal ,fluorocarbon nanoparticles ,ATHEROSCLEROSIS ,Heart failure ,Microbubbles ,VISUALIZATION ,Nanoparticles ,myocardial fibrosis ,Myocardial fibrosis ,CONTRAST AGENTS ,Collagen ,Rabbits ,Molecular imaging ,0210 nano-technology ,business ,Cardiomyopathies ,SUDDEN CARDIAC DEATH ,Biomedical engineering - Abstract
Myocardial fibrosis plays an important role in the development of heart failure and malignant arrhythmia, which potentially increases the incidence of sudden cardiac death. Therefore, early detection of myocardial fibrosis is of great significance for evaluating the prognosis of patients and formulating appropriate treatment strategies. Late gadolinium enhanced magnetic resonance imaging is considered as the currently effective strategy for noninvasive detection of myocardial fibrosis, but it still suffers from some critical issues. In this work, multifunctional CNA35-labeled perfluoropentane nanoparticles (CNA35-PFP NPs) have been elaborately designed and constructed for molecular imaging of fibrotic myocardium based on ultrasound imaging. These as-constructed CNA35-PFP NPs are intravenously infused into rabbit circulation with an animal model of myocardial infarction. Especially, these targeted CNA35-PFP NPs with nanoscale size could efficiently pass through the endothelial cell gap and adhere to the surface of fibroblasts in the fibrotic myocardium. Importantly, followed by low-intensity focused ultrasound irradiation on the myocardium, these intriguing CNA35-PFP NPs could transform from liquid into gaseous microbubbles, which further significantly enhanced the ultrasound contrast in the fibrotic area, facilitating the detection by diagnostic ultrasound imaging. Therefore, this work provides a desirable noninvasive, economical, and real-time imaging technique for the assessment of cardiac fibrosis with diagnostic ultrasound based on the rational design of liquid-to-gas phase-changeable nanoplatforms. more...
- Published
- 2019