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Made available in DSpace on 2022-04-28T19:44:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Brucellosis is a zoonotic disease with a global impact. Brucellasuis is one of the most pathogenic species to humans, requiring different measures for the control and/or eradication of the disease. The serological investigation for brucellosis was performed in pigs, horses, dogs, and cattle on a farm with a history of abortion in sows and necropsy of a boar with severe necrosuppurative orchitis. One sow, two cows, and two dogs reveled positive to Rose Bengal Test (RBT), although only the sow had a confirmatory outcome in 2-mercaptoethanol (2-ME). The 2-ME-positive sow was euthanized and microbiological culture of lymph nodes and liver followed by biochemical characterization allowed phenotypic characterization of Brucellasuis biotype 1. PCR multiplex Bruce-ladder and Suis-ladder enabled molecular confirmation, respectively, of Brucellasuis and biotype 1. The transmission aspects of B.suis to pigs and other domestic species, the combination of diagnostic procedures to diagnosis, as well as human health concerns of brucellosis are discussed. Department of Animal Production and Preventive Veterinary Medicine Faculty of Veterinary Medicine and Zootechnics State University of Sao Paulo, SP Department of Veterinary Clinical Science Federal University of Jataí, GO

Background : Calcinosis cutis is an uncommon dermatopathy characterized by the deposition of minerals in the skin, usually involving collagen and elastic fibers in the dermis. Usually, it results from dystrophic calcification and can be generalized or focal. The dermatopathy may be primary or secondary to certain disorders, especially chronic proliferative otitis, foreign body reactions, hyperadrenocorticism (HAC) and less frequently percutaneous penetration of calcium-rich products. The aim of this report is to describe a presentation of calcinosis cutis affecting the skin of the back, internal face of hind limbs and anal region of a 9-year-old bitch. Case : A 9-year-old, non-defined breed, bitch, ovariohysterectomized, weighing 9.45 kg, was attended at the Dermatological Service of companion animals at the Veterinary Hospital of the Federal University of Jatai (UFJ). The animal came in with the complaint of extensive dorsal alopecia, covered by firm lesions, with a 3-month evolution, additionally to polyuria and polydipsia. After physical examination, alopecic areas of great extension were confirmed on the dorsum, on the internal surface of the hind limbs and in the anal region. Also, an exudative and painful lesion located on the back was detected, plus loss of elasticity of the ventral abdomen skin and visible abdominal vessels. The screening tests showed a marked increase in the alanine aminotransferase enzyme (ALT), alkaline phosphatase (ALP) and total cholesterol. The specific urinary density was decreased. On the ultrasound examination, hepatomegaly and an increase in the caudal pole of the left adrenal were detected. Based on these findings, calcinosis cutis secondary to spontaneous hyperadrenocorticism (HAC) was suspected. For confirmation, skin biopsy and low dose dexamethasone suppression test (LDDS) were performed. LDDS test showed no reduction of serum cortisol after 8 h of dexamethasone dose administration and histopathological evaluation revealed multiple foci of calcinosis characterized by the deposition of basophilic material on the pre-existing collagen fibers, plus areas with pyogranulomatous inflammatory reaction and peripheral fibrosis with transepidermal elimination of minerals. Thus, trilostane and intense hydration of skin plaques were applied as treatment. Discussion : The dermatological alterations were compatible with those described in the consulted literature, with remarkable yellow-brown, firm, sandy-looking plaques, located on the back, internal face of hind limbs and anal region, possibly related to HAC later confirmed by LDDS test and biopsy. The management of the underlying disease and possible secondary bacterial infections are the basis of treatment. Therefore, the patient was treated with trilostane, antibiotic therapy and intensive hydration of the mineralized plaques resulting in a satisfactory involution of the clinical signs. Even though there are reports of calcinosis cutis on the dorsum, in the consulted literature there was no evidence of dorsum large extension lesion due to HAC as in this case report, but secondary to exogenous corticosteroid treatment, systemic blastomycosis and leptospirosis. In this case report, the affected thorax portion was the dorsum, differently from a study that pointed the ventral thorax as the affected portion. Similarly, anus and ventral part of the tail were hardly affected together with secondary inflammation and ulceration. Thus, the existent literature shows areas of calcinosis cutis in dogs in different parts of the body, but neither extensive as in the back of this reported female dog, nor widely affected as in the anal area, additionally to the internal face of hind limbs as already reported in the literature.

Feline chronic gingivostomatitis (FCGS) is a challenge for the veterinary practitioner since its etiology and treatments are still undefined. The present paper investigated the role of the feline immunodeficiency virus (FIV) in the severity of the FCGS. Oral mucosal biopsies obtained from 19 cats with FCGS were divided into two groups according to their FIV serology status. Later, the clinical lesion score was correlated with the histopathological grade of FCGS lesions and the degree of immunostaining in both groups. Most of the animals had significant histological changes; however, no correlation with FIV immunostaining intensity was observed. It was concluded that the presence of FIV infection or the animal’s seropositivity status does not seem to interfere with the severity of clinical signs nor the degree of histopathological changes when compared to the seronegative group.

Mast cell tumors represent the most common malignant skin tumor in the dog. This review outlines the incidence, etiology and clinical signs of mast cell tumors. Diagnostic tests, staging and treatments are also discussed. This study was performed by the Veterinary Oncology and Pathology of UNESP, Jaboticabal and the Service of Pathology Veterinary, of UNESP-Botucatu with the support of the Brazilian Association of Veterinary Oncology and the Brazilian Association of Veterinary Pathology.

Meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) is an important neurological disease that affects Brazilian cattle herds. The present study investigated the presence of BoHV-5 DNA in cattle diagnosed with meningoencephalitis at Faculdade de Medicina Veterinária e Zootecnia, UNESP - Univ Estadual Paulista from 1980 to 2009. The records obtained from the Large Animal Internal Medicine Service and the Animal Pathology Service were reviewed to identify clinical and epidemiological data from cattle with neurological signs. Excluding rabies cases, we found 115 cases of cattle with neurological signs that had been necropsied. Non-suppurative meningoencephalitis was diagnosed in 28 animals of the 115 initially selected based on histopathological examination of brain tissues. Of these 28 animals, 15 (54%) were positive for BoHV-5 DNA by polymerase chain reaction (PCR) of formalin-fixed paraffin-embedded (FFPE) brain samples. PCR target was 159-bp fragment from the BoHV-5 glycoprotein C gene. The oldest case identified in the present study was from 1988. PCR was a good tool for the diagnosis of BoHV-5 DNA extracted from FFPE tissues, allowing retrospective studies of samples stored for more than 20 years. RESUMO A meningoencefalite por herpesvírus bovino-5 (BoHV-5) é uma doença neurológica importante no rebanho bovino brasileiro. Este estudo tem por objetivo verificar a presença do DNA de BoHV-5 em bovinos diagnosticados com meningoencefalite na Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista, entre os anos de 1980 e 2009. Foram revisados os arquivos do Serviço de Clínica de Grandes Animais e da Patologia Animal em busca dos dados clínicos e epidemiológicos de bovinos com sinais neurológicos. Excluídos os casos de raiva, foram encontrados 115 casos de bovinos com sinais neurológicos, que foram necropsiados. O exame histopatológico realizado nos tecidos encefálicos desses animais constatou lesões de meningoencefalite não supurativa em 28 animais. Destes, em 15 (54%) casos foi identificada a presença do DNA de BoHV-5 por meio de PCR realizada em amostras de tecido encefálico fixadas em formalina e incluídas em parafina (FFPE). O alvo da PCR foi um fragmento de 159 pb do gene da glicoproteína C do BoHV-5. O caso mais antigo identificado neste estudo foi de 1988. A PCR apresentou-se como boa ferramenta para o diagnóstico do DNA de BoHV-5 extraído de tecidos FFPE, possibilitando estudos retrospectivos e diagnóstico de amostras com mais de 20 anos de armazenamento.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:56:30Z No. of bitstreams: 1 S0102-09352011000300030.pdf: 140179 bytes, checksum: 9296f71faca90d4433c6fae9c04a24e3 (MD5) Made available in DSpace on 2013-08-22T18:56:30Z (GMT). No. of bitstreams: 1 S0102-09352011000300030.pdf: 140179 bytes, checksum: 9296f71faca90d4433c6fae9c04a24e3 (MD5) Previous issue date: 2011-06-01 Made available in DSpace on 2013-09-30T19:50:30Z (GMT). No. of bitstreams: 2 S0102-09352011000300030.pdf: 140179 bytes, checksum: 9296f71faca90d4433c6fae9c04a24e3 (MD5) S0102-09352011000300030.pdf.txt: 13168 bytes, checksum: 337752d64dc95cc56a7048e773521064 (MD5) Previous issue date: 2011-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:41:50Z No. of bitstreams: 2 S0102-09352011000300030.pdf: 140179 bytes, checksum: 9296f71faca90d4433c6fae9c04a24e3 (MD5) S0102-09352011000300030.pdf.txt: 13168 bytes, checksum: 337752d64dc95cc56a7048e773521064 (MD5) Made available in DSpace on 2014-05-20T13:41:50Z (GMT). No. of bitstreams: 2 S0102-09352011000300030.pdf: 140179 bytes, checksum: 9296f71faca90d4433c6fae9c04a24e3 (MD5) S0102-09352011000300030.pdf.txt: 13168 bytes, checksum: 337752d64dc95cc56a7048e773521064 (MD5) Previous issue date: 2011-06-01 É descrito o carcinoma de células escamosas (CCE) no fundo vaginal de uma vaca. O diagnóstico de CCE moderadamente diferenciado foi confirmado através do exame histopatológico. Os testes imunoistoquímicos com os marcadores p53 e Ki67 realizados em amostras do tumor confirmaram a mutação na p53 e aumento da proliferação celular. Faculdade de Medicina Veterinária e Zootecnia - UNESP, Campus de Botucatu Faculdade de Medicina Veterinária e Zootecnia - UNESP, Campus de Botucatu

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:50:56Z No. of bitstreams: 1 S0100-736X2011000100007.pdf: 3278844 bytes, checksum: f9e09c4b9efeeba682c205596d3fbfcd (MD5) Made available in DSpace on 2013-08-22T18:50:56Z (GMT). No. of bitstreams: 1 S0100-736X2011000100007.pdf: 3278844 bytes, checksum: f9e09c4b9efeeba682c205596d3fbfcd (MD5) Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T18:18:03Z (GMT). No. of bitstreams: 2 S0100-736X2011000100007.pdf: 3278844 bytes, checksum: f9e09c4b9efeeba682c205596d3fbfcd (MD5) S0100-736X2011000100007.pdf.txt: 70398 bytes, checksum: ee986a3b54e2b3c4f50d9521b40a7a02 (MD5) Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:33:47Z No. of bitstreams: 2 S0100-736X2011000100007.pdf: 3278844 bytes, checksum: f9e09c4b9efeeba682c205596d3fbfcd (MD5) S0100-736X2011000100007.pdf.txt: 70398 bytes, checksum: ee986a3b54e2b3c4f50d9521b40a7a02 (MD5) Made available in DSpace on 2014-05-20T13:33:47Z (GMT). No. of bitstreams: 2 S0100-736X2011000100007.pdf: 3278844 bytes, checksum: f9e09c4b9efeeba682c205596d3fbfcd (MD5) S0100-736X2011000100007.pdf.txt: 70398 bytes, checksum: ee986a3b54e2b3c4f50d9521b40a7a02 (MD5) Previous issue date: 2011-01-01 O presente trabalho teve como objetivos avaliar os sinais clínicos, as concentrações do sulfeto de hidrogênio ruminal e as alterações anatomopatológicas associadas à intoxicação experimental por enxofre em bovinos. Foram utilizados dez bezerros mestiços leiteiros, sendo que quatro bovinos ingeriram ração sem sulfato de sódio (G1) e seis consumiram ração com sulfato de sódio (G2). Exames clínicos (temperatura retal, frequência cardíaca e respiratória e motricidade ruminal) e laboratoriais (hemograma, fibrinogênio, proteína plasmática, pH do fluido ruminal, concentração do sulfeto de hidrogênio ruminal, líquido cerebrospinal e histopatológico) foram realizados. A temperatura retal, frequência cardíaca, hemograma, fibrinogênio, proteína plasmática, pH do fluido ruminal e os valores do líquido cerebrospinal estavam dentro dos valores de referência para a espécie. Taquipnéia, hipomotricidade ruminal e elevados valores de sulfeto de hidrogênio ruminal foram observados nos bezerros do grupo G2. Um bezerro do grupo G2 apresentou sinais neurológicos e lesões histopatológicas de PEM. Dois animais de cada grupo foram eutanasiados. Lesões microscópicas foram observadas nos bezerros do G2. Histologicamente as alterações observadas foram necrose neuronal cortical e lesões hemorrágicas nos núcleos basais, tálamo, mesencéfalo, ponte e bulbo. O protocolo experimental constituído por uma dieta rica em carboidrato de alta fermentação, baixa quantidade de fibra efetiva e altos níveis de enxofre (0,52%) ocasionou alterações clinicas e histológicas e elevadas concentrações de sulfeto de hidrogênio ruminal compatíveis com quadro de intoxicação por enxofre. The aims of this study were to evaluate the clinical signs, the ruminal hydrogen sulfide concentration and the histological lesions induced by sulfur toxicosis in cattle. Ten crossbred calves were fed an experimental diet, four without sodium sulfate (G1) and six with (G2). The calves were submitted to clinical (rectal temperature, cardiac and respiratory rate and ruminal motricity) and laboratorial (hemogram, fibrinogen, total plasma protein, ruminal fluid pH, ruminal hydrogen sulfide concentration, cerebrospinal fluid and histopathological) evaluations. Rectal temperature, cardiac rate, hemogram, fibrinogen, total plasma protein, ruminal fluid pH and cerebrospinal fluid values were within normal reference ranges in animals from both groups. Ruminal hypomotricity and increased respiratory rate and ruminal hydrogen sulfide concentration occurred in G2 animals. One out of six calves in G2 developed neurological signs and lesions of PEM. Two calves of each Group were euthanized. Microscopic lesions of PEM were observed in G2 animals. Histologically there were cortical neuronal necrosis and hemorrhagic lesions in basal nuclei, thalamus, midbrain, pons and medulla oblongata. The experimental model consisting of a diet with high carbohydrate and low in long fiber content with high sulfur concentrations (0.52%) resulted in clinical and histological abnormalities and high ruminal hydrogen sulfide concentration consistent with sulpur toxicosis in cattle. Universidade Federal de Goiás (UFG) Escola de Veterinária Depto Medicina Veterinária Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Clínica Veterinária Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Clínica Veterinária

Made available in DSpace on 2015-10-21T13:09:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-04-01. Added 1 bitstream(s) on 2015-10-22T09:53:11Z : No. of bitstreams: 1 S0100-736X2015000400337.pdf: 409488 bytes, checksum: 55affbb4b281ddd22ce343505f842431 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A meningoencefalite bovina causada pelo BHV-5, um vírus DNA fita dupla envelopado que pertence à família Herpesviridae e subfamília Alphaherpesvirinae, é um importante diagnóstico diferencial das doenças do sistema nervoso central. O objetivo deste estudo foi descrever as alterações histológicas no sistema nervoso central de bovinos experimentalmente infectados com BHV-5 e comparar estas alterações com os resultados de imunoistoquímica (IHQ) e PCR. Amostras do sistema nervoso central de bezerros previamente inoculados com BHV-5 foram microscopicamente avaliadas e submetidas à IHQ e PCR. Todos os animais apresentaram meningoencefalite não-supurativa. Das 18 áreas avaliadas de cada bezerro, 32,41% e 35,13% foram positivas na IHQ e PCR, respectivamente. O telencéfalo apresentou lesões mais acentuadas e foi mais positivo na PCR do que as demais áreas encefálicas e se apresentou como a melhor área para coleta de material para o diagnóstico. As áreas positivas na IHQ e na PCR apresentaram lesões mais acentuadas do que as áreas negativas para as mesmas técnicas. O animal com sinais neurológicos apresentou mais áreas positivas para PCR e IHQ do que os demais animais. Bovine meningoencephalitis caused by BHV-5, a double-stranded DNA enveloped virus that belongs to the family Herpesviridae and subfamily Alphaherpesvirinae, is an important differential diagnosis of central nervous diseases. The aim of this study was to describe the histological changes in the central nervous system of calves experimentally infected with BHV-5 and compare these changes with the PCR and IHC results. Formalin-fixed paraffin- embedded central nervous system samples from calves previously inoculated with BHV-5 were microscopically evaluated and tested using IHC and PCR. All the animals presented with nonsuppurative meningoencephalitis. From 18 evaluated areas of each calf, 32.41% and 35.19% were positive by IHC and PCR, respectively. The telencephalon presented more accentuated lesions and positive areas in the PCR than other encephalic areas and was the best sampling area for diagnostic purposes. Positive areas in the IHC and PCR were more injured than IHC and PCR negative areas. The animal with neurological signs showed more PCR- and IHC-positive areas than the other animals. Univ Estadual Paulista Unesp, Fac Med Vet &Zootecnia, Dept Clin Vet, BR-18618970 Botucatu, SP, Brazil Univ Fed Goias, Escola Vet Zootecnia, Dept Med Vet, BR-74001970 Goiania, Go, Brazil FMVZ Unesp, Dept Higiene Vet &Saude Publ, Botucatu, SP, Brazil Univ Estadual Londrina, Dept Vet Prevent Med, Ctr Ciencias Agr, Lab Virol Anim, BR-86057970 Londrina, PR, Brazil Univ Estadual Paulista Unesp, Fac Med Vet &Zootecnia, Dept Clin Vet, BR-18618970 Botucatu, SP, Brazil FMVZ Unesp, Dept Higiene Vet & Saude Publ, Botucatu, SP, Brazil FAPESP: 2008/07697-9

São descritos dois surtos de intoxicação por Baccharis coridifolia em bovinos no Sul do Brasil. Os casos ocorreram quando bovinos estressados, com fome e sede, provenientes de pastagens livres de B. coridifolia, foram introduzidos em locais altamente infestados por essa planta tóxica. A morbidade nos dois surtos foi de 21,73% e 22,51%, e a letalidade foi virtualmente de 100%. Os sinais clínicos incluíam discreto timpanismo, instabilidade dos membros pélvicos, tremores musculares, focinho seco, fezes secas ou diarréicas, polidipsia e inquietação. Alterações presentes em todas as necropsias incluíam desidratação, grande quantidade de conteúdo líquido no rúmen, avermelhamento e erosões da mucosa dos pré-estômagos. As principais alterações histopatológicas encontradas foram degeneração e necrose do revestimento epitelial dos pré-estômagos e do tecido linfóide. Two outbreaks of Baccharis coridifolia poisoning in cattle in southern Brazil are described. Cases occurred when stressed, hungry and thirsty cattle brought from pastures free of B. coridifolia were placed into pastures heavily infested by this poisonous plant. In the two outbreaks morbidity was 21.73% and 22.51% and lethality was virtually 100%. Clinical signs included mild bloat, instability of hind limbs, muscle tremors, dry muzzle, dry feces or diarrhea, polydipsia and restlessness. Consistent necropsy findings included dehydration, large amounts of ruminal fluid, reddening and erosions of the mucosae of the forestomachs. Degeneration and necrosis of the lining epithelium of the forestomachs and of lymphoid tissue were the main histopathological changes encountered.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:29:49Z No. of bitstreams: 0 Made available in DSpace on 2014-05-27T11:29:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-07-01 Two Quarter Horse mares with hereditary equine regional dermal asthenia (HERDA) were diagnosed with metastatic squamous cell carcinoma (SCC) associated with chronic nonhealing wounds. The lesions were similar to the development of SCC from chronic nonhealing ulcers, known as Marjolin's ulcers in humans. The horses showed recurrent skin wounds in the saddle and paralumbar regions and were confirmed by molecular techniques as having HERDA. Both horses were maintained as research animals for prolonged periods and received regular veterinary care and wound treatment. Both horses were ultimately euthanized because of their chronic progressive wounds, coupled with declining health. At necropsy, the nonhealing wounds were found to be complicated by infiltrative SCC; both horses had metastasis to lungs. Chronically inflamed, recurrent skin wounds that heal slowly and incompletely as a consequence of HERDA are proposed as a major pathogenetic factor in tumorigenesis. Consistent findings with respect to proliferation index (Ki-67) and mutations of p53 tumor suppressor gene were confirmed by immunohistochemistry in one horse. SCC consistent with Marjolin's ulcer has been previously suggested in association with chronic ulcers or burn scars in horses, but this is the first report of an association with chronic poor healing wounds in HERDA horses. © 2013 Elsevier Inc. Department of Veterinary Clinical Science School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP), Botucatu, São Paulo Department of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University, East Lansing, MI Department of Pathobiology and Population Medicine Mississippi State University, Mississippi State, MS Department of Veterinary Medicine School of Veterinary Medicine Universidade Federal de Goiás (UFG), Goiânia, Goiás Department of Small Animal Clinical Sciences College of Veterinary Medicine Michigan State University, East Lansing, MI Department of Veterinary Medicine Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais Department of Clinical Sciences Mississippi State University, Mississippi, MS Department of Veterinary Clinical Science School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP), Botucatu, São Paulo

Made available in DSpace on 2013-08-28T14:07:58Z (GMT). No. of bitstreams: 1 WOS000312222400001.pdf: 1378925 bytes, checksum: dfe284238e8b3bb755ddd19343c5e77a (MD5) Made available in DSpace on 2013-09-30T18:26:11Z (GMT). No. of bitstreams: 2 WOS000312222400001.pdf: 1378925 bytes, checksum: dfe284238e8b3bb755ddd19343c5e77a (MD5) WOS000312222400001.pdf.txt: 27115 bytes, checksum: d4324702adf21e0f5e43526bd60f7a04 (MD5) Previous issue date: 2012-09-25 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:37:16Z No. of bitstreams: 2 WOS000312222400001.pdf: 1378925 bytes, checksum: dfe284238e8b3bb755ddd19343c5e77a (MD5) WOS000312222400001.pdf.txt: 27115 bytes, checksum: d4324702adf21e0f5e43526bd60f7a04 (MD5) Made available in DSpace on 2014-05-20T13:37:16Z (GMT). No. of bitstreams: 2 WOS000312222400001.pdf: 1378925 bytes, checksum: dfe284238e8b3bb755ddd19343c5e77a (MD5) WOS000312222400001.pdf.txt: 27115 bytes, checksum: d4324702adf21e0f5e43526bd60f7a04 (MD5) Previous issue date: 2012-09-25 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Reports of neoplasms in Panthera species are increasing, but they are still an uncommon cause of disease and death in captive wild felids. The presence of two or more primary tumor in large felids is rarely reported, and there are no documented cases of ocular melanoma and mammary mucinous carcinoma in African lions.Case presentation: An ocular melanoma and a mammary mucinous carcinoma are described in an African lion (Panthera leo). The first tumour was histologically characterized by the presence of epithelioid and fusiform melanocytes, while the latter was composed of mucus-producing cells with an epithelial phenotype that contained periodic acid-Schiff (PAS) and Alcian blue staining mucins. Metastases of both tumor were identified in various organs and indirect immunohistochemistry was used to characterize them. Peribiliary cysts were observed in the liver.Conclusions: This is the first description of these tumor in African lions. Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, Dept Clin Vet, Botucatu, SP, Brazil Univ Estadual Paulista UNESP, Fac Med Botucatu, Dept Patol, Botucatu, SP, Brazil FEPI, Curso Med Vet, Itajuba, Brazil Publ Vet Hosp, Dept Diagnost Pathol, Vet Serv, Natl Assoc Small Anim Clinicians Anclivepa, São Paulo, Brazil Hosp AC Camargo Fundação Antonio Prudente, Dept Anat Patol, São Paulo, Brazil Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, Dept Clin Vet, Botucatu, SP, Brazil Univ Estadual Paulista UNESP, Fac Med Botucatu, Dept Patol, Botucatu, SP, Brazil CNPq: 479178/2010-0 FAPESP: 09/53493-9 FAPESP: 10/51596-2 FAPESP: 11/01610-1

Made available in DSpace on 2013-09-27T14:54:15Z (GMT). No. of bitstreams: 1 WOS000297936600034.pdf: 1327280 bytes, checksum: fe7f0fdb2447823623373b7836ec30a2 (MD5) Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T18:26:15Z (GMT). No. of bitstreams: 1 WOS000297936600034.pdf: 1327280 bytes, checksum: fe7f0fdb2447823623373b7836ec30a2 (MD5) Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:44Z No. of bitstreams: 1 WOS000297936600034.pdf: 1327280 bytes, checksum: fe7f0fdb2447823623373b7836ec30a2 (MD5) Made available in DSpace on 2014-05-20T13:39:44Z (GMT). No. of bitstreams: 1 WOS000297936600034.pdf: 1327280 bytes, checksum: fe7f0fdb2447823623373b7836ec30a2 (MD5) Previous issue date: 2011-01-01 The pathological findings in a 2-years-old Syrian hamster (Mesocricetus auratus) with a cutaneous myxosarcoma are described. Grossly, there was a large cutaneous mass in the right cervical region. Microscopical evaluation revealed a myxosarcoma characterized by pleomorphic, fusiform cells loosely arranged, randomly distributed, and presenting a moderate amount of basophilic amorphous stroma. There were hemorrhagic areas within the tumor. The basophilic amorphous stroma was positive to Alcian blue confirming the presence of a mucopolysaccharide matrix. Immunohistochemically, the neoplastic cells expressed vimentin, and were negative for cytokeratin or glial fibrillary acidic protein (GFAP). UNESP, Programa Posgrad Med Vet, FMVZ, Botucatu, SP, Brazil UNESP, Fac Integradas Ourinhos, Botucatu, SP, Brazil UNESP, Depto Clin Vet, Lab Patol Vet, FMVZ, Botucatu, SP, Brazil UNESP, Programa Posgrad Med Vet, FMVZ, Botucatu, SP, Brazil UNESP, Fac Integradas Ourinhos, Botucatu, SP, Brazil UNESP, Depto Clin Vet, Lab Patol Vet, FMVZ, Botucatu, SP, Brazil

The present study reports the occurrence of lead poisoning in a male, 2 year-old mongrel dog attended at UNESP Botucatu Veterinary Hospital. The animal was brought to the hospital with claim of gastrointestinal and nervous disturbs for 10 days, worsening in the last 24 hours. After unsuccessfully attempts to control status epileticus, the animal died. Hematological evaluation revealed significant rubricytosis and basophilic stippling, highly suggestive of lead poisoning. Serum lead concentration confirmed toxic levels of this element. Necropsy evidenced the presence of foreign bodies in stomach (one stone and two metallic objects) and, microscopically, characteristic inclusions in renal parenchyma. Fragments obtained from liver and kidneys revealed lead concentrations of 0, 8 μg/g and 25 μg/g, respectively, which in association to clinical signs of saturnism, determined the diagnosis of lead poisoning.