164 results on '"Daniel Batlle"'
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2. Ang II (Angiotensin II) Conversion to Angiotensin-(1-7) in the Circulation Is POP (Prolyloligopeptidase)-Dependent and ACE2 (Angiotensin-Converting Enzyme 2)-Independent
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J. Arturo García-Horsman, Jan Wysocki, Gvantca Gulua, Arndt Schulze, Peter Serfozo, Minghao Ye, Timo T. Myöhänen, Pan Liu, Michael Bader, Daniel Batlle, Jing Jin, Faculty of Pharmacy, Regenerative pharmacology group, Drug Research Program, University Management, PREP in neurodegenerative disorders, Division of Pharmacology and Pharmacotherapy, and Helsinki In Vivo Animal Imaging Platform (HAIP)
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Male ,0301 basic medicine ,medicine.medical_specialty ,Angiotensins ,Blood Pressure ,Carboxypeptidases ,Peptidyl-Dipeptidase A ,030204 cardiovascular system & hematology ,Article ,Renin-Angiotensin System ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,medicine ,Animals ,Mice, Knockout ,chemistry.chemical_classification ,Angiotensin 1 ,Angiotensin II ,Serine Endopeptidases ,Peptide Fragments ,030104 developmental biology ,Enzyme ,Endocrinology ,chemistry ,Prolyl oligopeptidase ,Hypertension ,Angiotensin-converting enzyme 2 ,cardiovascular system ,1182 Biochemistry, cell and molecular biology ,Angiotensin-Converting Enzyme 2 ,Angiotensin I ,Prolyl Oligopeptidases ,hormones, hormone substitutes, and hormone antagonists - Abstract
The Ang II (Angiotensin II)-Angiotensin-(1-7) axis of the Renin Angiotensin System encompasses 3 enzymes that form Angiotensin-(1-7) [Ang-(1-7)] directly from Ang II: ACE2 (angiotensin-converting enzyme 2), PRCP (prolylcarboxypeptidase), and POP (prolyloligopeptidase). We investigated their relative contribution to Ang-(1-7) formation in vivo and also ex vivo in serum, lungs, and kidneys using models of genetic ablation coupled with pharmacological inhibitors. In wild-type (WT) mice, infusion of Ang II resulted in a rapid increase of plasma Ang-(1-7). In ACE2 −/− / PRCP −/− mice, Ang II infusion resulted in a similar increase in Ang-(1-7) as in WT (563±48 versus 537±70 fmol/mL, respectively), showing that the bulk of Ang-(1-7) formation in circulation is essentially independent of ACE2 and PRCP. By contrast, a POP inhibitor, Z-Pro-Prolinal reduced the rise in plasma Ang-(1-7) after infusing Ang II to control WT mice. In POP −/− mice, the increase in Ang-(1-7) was also blunted as compared with WT mice (309±46 and 472±28 fmol/mL, respectively P =0.01), and moreover, the rate of recovery from acute Ang II-induced hypertension was delayed ( P =0.016). In ex vivo studies, POP inhibition with ZZP reduced Ang-(1-7) formation from Ang II markedly in serum and in lung lysates. By contrast, in kidney lysates, the absence of ACE2, but not POP, obliterated Ang-(1-7) formation from added Ang II. We conclude that POP is the main enzyme responsible for Ang II conversion to Ang-(1-7) in the circulation and in the lungs, whereas Ang-(1-7) formation in the kidney is mainly ACE2-dependent.
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- 2020
3. Progressive Hypertension and Severe Left Ventricular Outflow Tract Obstruction
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Sandra J. Taler, John S. Floras, Alan C. Cameron, Atul R. Chugh, Ninian N. Lang, Daniel Batlle, Christian Delles, Michael Bursztyn, Garry L. Jennings, Anna F. Dominiczak, and Rhian M. Touyz
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medicine.medical_specialty ,Time Factors ,Ventricular outflow tract obstruction ,Severity of Illness Index ,Ventricular Outflow Obstruction ,Internal medicine ,Severity of illness ,Internal Medicine ,medicine ,Humans ,Blood pressure monitoring ,Obesity ,Hypertension diagnosis ,Aged ,business.industry ,Disease progression ,Follow up studies ,Blood Pressure Monitoring, Ambulatory ,Echocardiography, Doppler ,Hypertension complications ,Hypertension ,Disease Progression ,Cardiology ,Female ,medicine.symptom ,business ,Follow-Up Studies - Abstract
No abstract available.
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- 2019
4. Evidence For and Against Direct Kidney Infection by SARS-CoV-2 in Patients with COVID-19
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Fabiola Reyes, Paul A. Welling, Luise Hassler, Daniel Batlle, and Matthew A. Sparks
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Feature ,Pathology ,medicine.medical_specialty ,Epidemiology ,viruses ,Interstitial nephritis ,Biopsy ,Critical Care and Intensive Care Medicine ,Immunofluorescence ,Kidney ,Risk Assessment ,Pathogenesis ,COVID-19 Testing ,Predictive Value of Tests ,Risk Factors ,medicine ,Animals ,Humans ,Kidney infection ,Transplantation ,medicine.diagnostic_test ,business.industry ,SARS-CoV-2 ,Acute kidney injury ,COVID-19 ,medicine.disease ,Prognosis ,medicine.anatomical_structure ,Nephrology ,Host-Pathogen Interactions ,Kidney Diseases ,business ,Kidney disease - Abstract
Despite evidence of multiorgan tropism of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with coronavirus disease 2019 (COVID-19), direct viral kidney invasion has been difficult to demonstrate. The question of whether SARS-CoV2 can directly infect the kidney is relevant to the understanding of pathogenesis of AKI and collapsing glomerulopathy in patients with COVID-19. Methodologies to document SARS-CoV-2 infection that have been used include immunohistochemistry, immunofluorescence, RT-PCR, in situ hybridization, and electron microscopy. In our review of studies to date, we found that SARS-CoV-2 in the kidneys of patients with COVID-19 was detected in 18 of 94 (19%) by immunohistochemistry, 71 of 144 (49%) by RT-PCR, and 11 of 84 (13%) by in situ hybridization. In a smaller number of patients with COVID-19 examined by immunofluorescence, SARS-CoV-2 was detected in 10 of 13 (77%). In total, in kidneys from 102 of 235 patients (43%), the presence of SARS-CoV-2 was suggested by at least one of the methods used. Despite these positive findings, caution is needed because many other studies have been negative for SARS-CoV-2 and it should be noted that when detected, it was only in kidneys obtained at autopsy. There is a clear need for studies from kidney biopsies, including those performed at early stages of the COVID-19–associated kidney disease. Development of tests to detect kidney viral infection in urine samples would be more practical as a noninvasive way to evaluate SARS-CoV-2 infection during the evolution of COVID-19–associated kidney disease.
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- 2021
5. Proximal renal tubular acidosis with and without Fanconi syndrome
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Ibrahim Kashoor and Daniel Batlle
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medicine.medical_specialty ,lcsh:Internal medicine ,lcsh:Specialties of internal medicine ,030232 urology & nephrology ,Review Article ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,lcsh:RC581-951 ,Internal medicine ,medicine ,lcsh:RC31-1245 ,Kidney ,business.industry ,Reabsorption ,urogenital system ,renal tubular ,Fanconi syndrome ,Drug-induced nephrotoxicity ,Metabolic acidosis ,General Medicine ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,Convoluted tubule ,Renal physiology ,Cystinosis ,business ,Acidosis ,Proximal renal tubular acidosis ,Proximal tubular toxicity - Abstract
Proximal renal tubular acidosis (RTA) is caused by a defect in bicarbonate (HCO3−) reabsorption in the kidney proximal convoluted tubule. It usually manifests as normal anion-gap metabolic acidosis due to HCO3− wastage. In a normal kidney, the thick ascending limb of Henle’s loop and more distal nephron segments reclaim all of the HCO3− not absorbed by the proximal tubule. Bicarbonate wastage seen in type II RTA indicates that the proximal tubular defect is severe enough to overwhelm the capacity for HCO3− reabsorption beyond the proximal tubule. Proximal RTA can occur as an isolated syndrome or with other impairments in proximal tubular functions under the spectrum of Fanconi syndrome. Fanconi syndrome, which is characterized by a defect in proximal tubular reabsorption of glucose, amino acids, uric acid, phosphate, and HCO3−, can occur due to inherited or acquired causes. Primary inherited Fanconi syndrome is caused by a mutation in the sodium-phosphate cotransporter (NaPi-II) in the proximal tubule. Recent studies have identified new causes of Fanconi syndrome due to mutations in the EHHADH and the HNF4A genes. Fanconi syndrome can also be one of many manifestations of various inherited systemic diseases, such as cystinosis. Many of the acquired causes of Fanconi syndrome with or without proximal RTA are drug-induced, with the list of causative agents increasing as newer drugs are introduced for clinical use, mainly in the oncology field.
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- 2019
6. Review for 'Genotypic and phenotypic analysis in 51 Chinese patients with primary distal renal tubular acidosis'
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Daniel Batlle
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Pathology ,medicine.medical_specialty ,Phenotypic analysis ,Distal renal tubular acidosis ,business.industry ,Genotype ,Medicine ,business ,medicine.disease - Published
- 2021
7. A novel soluble ACE2 protein totally protects from lethal disease caused by SARS-CoV-2 infection
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Vlad Nicoleascu, Luise Hassler, Anastasia Tomatsidou, Daniel Batlle, Glenn Randall, Haley Gula, Jan Wysocki, Anjana V. Yeldandi, Jack Henkin, and Ian Gelarden
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medicine.medical_specialty ,Lung ,business.industry ,Transgene ,fungi ,Albumin ,Pharmacology ,Article ,medicine.anatomical_structure ,In vivo ,Viral entry ,Angiotensin-converting enzyme 2 ,medicine ,Histopathology ,business ,hormones, hormone substitutes, and hormone antagonists ,Binding domain - Abstract
Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) uses full-length angiotensin converting enzyme 2 (ACE2), which is membrane bound, as its initial cell contact receptor preceding viral entry. Here we report a human soluble ACE2 variant fused with a 5kD albumin binding domain (ABD) and bridged via a dimerization motif hinge-like 4-cysteine dodecapeptide, which we term ACE2 1-618-DDC-ABD. This protein is enzymatically active, has increased duration of action in vivo conferred by the ABD-tag, and displays 20-30-fold higher binding affinity to the SARS-CoV-2 receptor binding domain than its des-DDC monomeric form (ACE2 1-618-ABD) due to DDC-linked dimerization. ACE2 1-618-DDC-ABD was administered for 3 consecutive days to transgenic k18-hACE2 mice, a model that develops lethal SARS-CoV-2 infection, to evaluate the preclinical preventative/ therapeutic value for COVID-19. Mice treated with ACE2 1-618-DDC-ABD developed a mild to moderate disease for the first few days assessed by a clinical score and modest weight loss. The untreated control animals, by contrast, became severely ill and had to be sacrificed by day 6/7 and lung histology revealed extensive pulmonary alveolar hemorrhage and mononuclear infiltrates. At 6 days, mortality was totally prevented in the treated group, lung histopathology was improved and viral titers markedly reduced. This demonstrates for the first time in vivo the preventative/ therapeutic potential of a novel soluble ACE2 protein in a preclinical animal model.
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- 2021
8. Knockout of aminopeptidase A in mice causes functional alterations and morphological glomerular basement membrane changes in the kidneys
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Meei Hua Lin, Jan Wysocki, Benedikt Marahrens, Juan Carlos Q. Velez, Daniel Batlle, Minghao Ye, Yashpal S. Kanwar, Arndt Schulze, Jeffrey H. Miner, and Michael Bader
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0301 basic medicine ,Male ,medicine.medical_specialty ,030232 urology & nephrology ,Glutamyl Aminopeptidase ,Kidney ,Renin-Angiotensin System ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Internal medicine ,Renin–angiotensin system ,Glomerular Basement Membrane ,medicine ,Animals ,Mice, Knockout ,biology ,urogenital system ,Chemistry ,Glomerular basement membrane ,Angiotensin II ,Angiotensin-converting enzyme ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Nephrology ,Knockout mouse ,Albuminuria ,biology.protein ,Female ,medicine.symptom ,Homeostasis - Abstract
Aminopeptidase A is one of the most potent enzymes within the renin-angiotensin system in terms of angiotensin II degradation. Here, we examined whether there is a kidney phenotype and any compensatory changes in other renin angiotensin system enzymes involved in the metabolism of angiotensin II associated with aminopeptidase A deficiency. Kidneys harvested from aminopeptidase A knockout mice were examined by light and electron microscopy, immunohistochemistry and immunofluorescence. Kidney angiotensin II levels and the ability of renin angiotensin system enzymes in the glomerulus to degrade angiotensin II ex vivo, their activities, protein and mRNA levels in kidney lysates were evaluated. Knockout mice had increased blood pressure and mild glomerular mesangial expansion without significant albuminuria. By electron microscopy, knockout mice exhibited a mild increase of the mesangial matrix, moderate thickening of the glomerular basement membrane but a striking appearance of knob-like structures. These knobs were seen in both male and female mice and persisted after the treatment of hypertension. In isolated glomeruli from knockout mice, the level of angiotensin II was more than three-fold higher as compared to wild type control mice. In kidney lysates from knockout mice angiotensin converting enzyme activity, protein and mRNA levels were markedly decreased possibly as a compensatory mechanism to reduce angiotensin II formation. Thus, our findings support a role for aminopeptidase A in the maintenance of glomerular structure and intra-kidney homeostasis of angiotensin peptides.
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- 2020
9. The ACE2‐deficient mouse: A model for a cytokine storm‐driven inflammation
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Nihal Kaplan, Han Peng, Daniel Batlle, Jan Wysocki, Kurt Q. Lu, Wending Yang, Robert M. Lavker, and Junyi Wang
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0301 basic medicine ,THP-1 Cells ,medicine.medical_treatment ,Corneal inflammation ,Biochemistry ,SARS‐CoV‐2 ,Mice ,0302 clinical medicine ,Cornea ,Cells, Cultured ,Research Articles ,Corneal epithelium ,Mice, Knockout ,Angiotensin II ,macrophages ,Losartan ,medicine.anatomical_structure ,Cytokine ,Angiotensin-converting enzyme 2 ,Angiotensin-Converting Enzyme 2 ,Chemokines ,medicine.symptom ,Cytokine Release Syndrome ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,medicine.drug ,Biotechnology ,medicine.medical_specialty ,Inflammation ,03 medical and health sciences ,COVID‐19 ,cornea ,Internal medicine ,medicine ,Genetics ,Animals ,Humans ,Molecular Biology ,SARS-CoV-2 ,Tumor Necrosis Factor-alpha ,business.industry ,Interleukins ,COVID-19 ,Epithelial Cells ,medicine.disease ,eye diseases ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,corneal epithelial cells ,sense organs ,Cytokine storm ,business ,030217 neurology & neurosurgery - Abstract
Angiotensin converting enzyme 2 (ACE2) plays an important role in inflammation, which is attributable at least, in part, to the conversion of the pro‐inflammatory angiotensin (Ang) II peptide into angiotensin 1‐7 (Ang 1‐7), a peptide which opposes the actions of AngII. ACE2 and AngII are present in many tissues but information on the cornea is lacking. We observed that mice deficient in the Ace2 gene (Ace2−/−), developed a cloudy cornea phenotype as they aged. Haze occupied the central cornea, accompanied by corneal edema and neovascularization. In severe cases with marked chronic inflammation, a cell‐fate switch from a transparent corneal epithelium to a keratinized, stratified squamous, psoriasiform‐like epidermis was observed. The stroma contained a large number of CD11c, CD68, and CD3 positive cells. Corneal epithelial debridement experiments in young ACE2‐deficient mice showed normal appearing corneas, devoid of haze. We hypothesized, however, that these mice are “primed” for a corneal inflammatory response, which once initiated, would persist. In vitro studies reveal that interleukins (IL‐1a, IL‐1b), chemokines (CCL2, CXCL8), and TNF‐α, are all significantly elevated, resulting in a cytokine storm‐like phenotype. This phenotype could be partially rescued by treatment with the AngII type 1 receptor (AT1R) antagonist, losartan, suggesting that the observed effect was mediated by AngII acting on its main receptor. Since the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) utilizes human ACE2 as the receptor for entry with subsequent downregulation of ACE2, corneal inflammation in Ace2−/− mice may have a similar mechanism with that in COVID‐19 patients. Thus the Ace2−/− cornea, because of easy accessibility, may provide an attractive model to explore the molecular mechanisms, immunological changes, and treatment modalities in patients with COVID‐19.
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- 2020
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10. A Survey on Dialysis-Related Muscle Cramping and a Hypothesis of Angiotensin II on Its Pathophysiology
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Shweta Punj, Daniel Batlle, Arthur J. Atkinson, Alonso Marquez, and Alsoufi Enaam
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medicine.medical_specialty ,Nephrology ,business.industry ,Internal medicine ,medicine ,MEDLINE ,Research Letter ,Dialysis (biochemistry) ,business ,Angiotensin II ,Pathophysiology - Published
- 2019
11. Hypokalemic Distal Renal Tubular Acidosis
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Daniel Batlle and Patricia G. Vallés
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Vacuolar Proton-Translocating ATPases ,DISTAL RTA ,medicine.medical_specialty ,CIENCIAS MÉDICAS Y DE LA SALUD ,Urinary system ,GROWTH FAILURE ,030232 urology & nephrology ,Ciencias de la Salud ,Hypokalemia ,030204 cardiovascular system & hematology ,Carbonic Anhydrase II ,Excretion ,03 medical and health sciences ,0302 clinical medicine ,Distal renal tubular acidosis ,Anion Exchange Protein 1, Erythrocyte ,Internal medicine ,medicine ,Humans ,Hypercalciuria ,Kidney Tubules, Distal ,Acidosis ,ACID EXCRETION ,HYPOKALEMIA ,business.industry ,Biological Transport ,Metabolic acidosis ,Acidosis, Renal Tubular ,medicine.disease ,HYPERCHLOREMIC METABOLIC ACIDOSIS ,Otras Ciencias de la Salud ,Endocrinology ,Nephrology ,Mutation ,Potassium ,medicine.symptom ,Nephrocalcinosis ,business ,Glomerular Filtration Rate - Abstract
Distal renal tubular acidosis (DRTA) is defined as hyperchloremic, non-anion gap metabolic acidosis with impaired urinary acid excretion in the presence of a normal or moderately reduced glomerular filtration rate. Failure in urinary acid excretion results from reduced H+ secretion by intercalated cells in the distal nephron. This results in decreased excretion of NH4 + and other acids collectively referred as titratable acids while urine pH is typically above 5.5 in the face of systemic acidosis. The clinical phenotype in patients with DRTA is characterized by stunted growth with bone abnormalities in children as well as nephrocalcinosis and nephrolithiasis that develop as the consequence of hypercalciuria, hypocitraturia, and relatively alkaline urine. Hypokalemia is a striking finding that accounts for muscle weakness and requires continued treatment together with alkali-based therapies. This review will focus on the mechanisms responsible for impaired acid excretion and urinary potassium wastage, the clinical features, and diagnostic approaches of hypokalemic DRTA, both inherited and acquired. Fil: Garramuño, Patricia. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Battle, Daniel. Northwestern University; Estados Unidos
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- 2018
12. Urine RAS components in mice and people with type 1 diabetes and chronic kidney disease
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Anne Goodling, Jan Wysocki, John T. Ruzinski, Maryam Afkarian, Daniel Batlle, Kathryn B. Whitlock, and Mar Burgaya
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Adult ,Male ,0301 basic medicine ,medicine.medical_specialty ,Physiology ,Cathepsin D ,Angiotensin-Converting Enzyme Inhibitors ,Disease ,Urine ,Urinalysis ,030204 cardiovascular system & hematology ,Diabetes Mellitus, Experimental ,Renin-Angiotensin System ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Predictive Value of Tests ,Internal medicine ,Renin–angiotensin system ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,Renal Insufficiency, Chronic ,Type 1 diabetes ,biology ,Chemistry ,Angiotensin-converting enzyme ,Middle Aged ,medicine.disease ,Enzymes ,Up-Regulation ,Diabetes Mellitus, Type 1 ,030104 developmental biology ,Endocrinology ,Angiotensin-converting enzyme 2 ,biology.protein ,Female ,Biomarkers ,Research Article ,Kidney disease - Abstract
The pathways implicated in diabetic kidney disease (DKD) are largely derived from animal models. To examine if alterations in renin-angiotensin system (RAS) in humans are concordant with those in rodent models, we measured concentration of angiotensinogen (AOG), cathepsin D (CTSD), angiotensin-converting enzyme (ACE), and ACE2 and enzymatic activities of ACE, ACE2, and aminopeptidase-A in FVB mice 13–20 wk after treatment with streptozotocin ( n = 9) or vehicle ( n = 15) and people with long-standing type 1 diabetes, with ( n = 37) or without ( n = 81) DKD. In streptozotocin-treated mice, urine AOG and CTSD were 10.4- and 3.0-fold higher than in controls, respectively ( P < 0.001). Enzymatic activities of ACE, ACE2, and APA were 6.2-, 3.2-, and 18.8-fold higher, respectively, in diabetic animals ( P < 0.001). Angiotensin II was 2.4-fold higher in diabetic animals ( P = 0.017). Compared with people without DKD, those with DKD had higher urine AOG (170 vs. 15 μg/g) and CTSD (147 vs. 31 μg/g). In people with DKD, urine ACE concentration was 1.8-fold higher (1.4 vs. 0.8 μg/g in those without DKD), while its enzymatic activity was 0.6-fold lower (1.0 vs. 1.6 × 109RFU/g in those without DKD). Lower ACE activity, but not ACE protein concentration, was associated with ACE inhibitor (ACEI) treatment. After adjustment for clinical covariates, AOG, CTSD, ACE concentration, and ACE activity remained associated with DKD. In conclusion, in mice with streptozotocin-induced diabetes and in humans with DKD, urine concentrations and enzymatic activities of several RAS components are concordantly increased, consistent with enhanced RAS activity and greater angiotensin II formation. ACEI use was associated with a specific reduction in urine ACE activity, not ACE protein concentration, suggesting that it may be a marker of exposure to this widely-used therapy.
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- 2017
13. Angiotensin-converting enzyme 2 amplification limited to the circulation does not protect mice from development of diabetic nephropathy
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Mark J. Osborn, Minghao Ye, Agnes B. Fogo, Aline Martin, Jan Wysocki, Ahmed M Khattab, Daniel Batlle, Yashpal S. Kanwar, and Nicolae Valentin David
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0301 basic medicine ,medicine.medical_specialty ,Angiotensin II receptor type 1 ,Renal function ,030204 cardiovascular system & hematology ,Biology ,medicine.disease ,Angiotensin II ,Article ,Diabetic nephropathy ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Endocrinology ,Nephrology ,Internal medicine ,Diabetes mellitus ,Angiotensin-converting enzyme 2 ,Renin–angiotensin system ,medicine ,Albuminuria ,medicine.symptom ,hormones, hormone substitutes, and hormone antagonists - Abstract
Blockers of the renin-angiotensin system are effective in the treatment of experimental and clinical diabetic nephropathy. An approach different from blocking the formation or action of angiotensin II (1-8) that could also be effective involves fostering its degradation. Angiotensin-converting enzyme 2 (ACE2) is a monocarboxypeptidase that cleaves angiotensin II (1-8) to form angiotensin (1-7). Therefore, we examined the renal effects of murine recombinant ACE2 in mice with streptozotocin-induced diabetic nephropathy as well as that of amplification of circulating ACE2 using minicircle DNA delivery prior to induction of experimental diabetes. This delivery resulted in a long-term sustained and profound increase in serum ACE2 activity and enhanced ability to metabolize an acute angiotensin II (1-8) load. In mice with streptozotocin-induced diabetes pretreated with minicircle ACE2, ACE2 protein in plasma increased markedly and this was associated with a more than 100-fold increase in serum ACE2 activity. However, minicircle ACE2 did not result in changes in urinary ACE2 activity as compared to untreated diabetic mice. In both diabetic groups, glomerular filtration rate increased significantly and to the same extent as compared to non-diabetic controls. Albuminuria, glomerular mesangial expansion, glomerular cellularity, and glomerular size were all increased to a similar extent in minicircle ACE2-treated and untreated diabetic mice, as compared to non-diabetic controls. Recombinant mouse ACE2 given for 4 weeks by intraperitoneal daily injections in mice with streptozotocin-induced diabetic nephropathy also failed to improve albuminuria or kidney pathology. Thus, a profound augmentation of ACE2 confined to the circulation failed to ameliorate the glomerular lesions and hyperfiltration characteristic of early diabetic nephropathy. These findings emphasize the importance of targeting the kidney rather than the circulatory renin angiotensin system to combat diabetic nephropathy.
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- 2017
14. Hypertension and Its Complications in a Young Man With Autoimmune Disease
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Cheryl L. Laffer, Garry L. Jennings, Anna F. Dominiczak, Neeraj Dhaun, Eve Miller-Hodges, Fernando Elijovich, Suzanne Oparil, Anna Oliveras, Daniel Batlle, and Jan Basile
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Lupus nephritis ,Renal function ,Blood Pressure ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Antiphospholipid syndrome ,Internal Medicine ,medicine ,Humans ,Lupus Erythematosus, Systemic ,Microhematuria ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,030203 arthritis & rheumatology ,Lupus erythematosus ,medicine.diagnostic_test ,business.industry ,Renal vein thrombosis ,medicine.disease ,Lupus Nephritis ,medicine.icd_9_cm_classification ,Hypertension ,Renal biopsy ,business ,Nephrotic syndrome - Abstract
A 30-year-old man, who had moved to the United Kingdom from South Asia, was referred to the renal clinic with nephrotic syndrome. He had recently been diagnosed with systemic lupus erythematosus (SLE) after presenting to the rheumatology clinic with joint pain, skin rash, and pleuritic chest pain and fulfilling 8 of 17 SLICC (Systemic Lupus International Collaborating Clinics) diagnostic criteria.1 His immunology was in keeping with active SLE: his complement levels were low, and he had antibodies against double-stranded DNA and extractable nuclear antigens (Table S1A in the online-only Data Supplement). Our patient had heavy proteinuria (3.9 g/d) and a low serum albumin (25 g/L) in keeping with the nephrotic syndrome. Although his excretory renal function was normal, he had microhematuria (3+) on urinalysis. An urgent renal tract ultrasound with Doppler revealed that he had a preexistent renal vein thrombosis for which he was anticoagulated. In the absence of any serological evidence of antiphospholipid syndrome, this was attributed to his nephrotic syndrome. He went on to have a renal biopsy. This demonstrated classes III (focal proliferative) and V (membranous) lupus nephritis (Figure 1). Figure 1. Initial glomerular histology. A , Normal glomerulus. Single arrow: Normal capillary wall. This should be a similar thickness to the tubular epithelium (arrowhead). Double arrow: Normal mesangium. B , Classes III (focal proliferative) and V (membranous) lupus nephritis. Single arrow: Thickened capillary wall. Double arrow: Focal proliferation. At presentation, his blood pressure (BP) was 126/88 mm Hg in the absence of antihypertensive treatment. Although this lay within both UK and US recommended guidelines, …
- Published
- 2017
15. Hypokalemia associated with acute colonic pseudo-obstruction in an ESRD patient
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Daniel Batlle, Khaled Boobes, and Robert M. Rosa
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Male ,medicine.medical_specialty ,BK channel ,medicine.medical_treatment ,Colonic Pseudo-Obstruction ,030232 urology & nephrology ,Hypokalemia ,030204 cardiovascular system & hematology ,urologic and male genital diseases ,Gastroenterology ,Peritoneal dialysis ,Excretion ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Wasting ,biology ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Endocrinology ,Nephrology ,Etiology ,biology.protein ,Kidney Failure, Chronic ,medicine.symptom ,business ,Peritoneal Dialysis ,Kidney disease - Abstract
Ogilvie's syndrome, or acute colonic pseudo-obstruction, is characterized by massive dilation of the colon without mechanical obstruction. Water and electrolytes often can be sequestered in the dilated intestinal loops resulting in profuse and watery diarrhea as well as hypokalemia. We report an anuric, end-stage renal disease (ESRD) patient undergoing peritoneal dialysis (PD) who developed acute colonic pseudo-obstruction causing a prolonged hospitalization. He also developed severe hypokalemia with a serum potassium (K+) as low as 2.4 mEq/L and required 180 - 240 mEq of potassium chloride per day for more than a month to correct it. While PD K+ losses often contribute to hypokalemia, the PD K+ loss was estimated to be only 39 mEq/day. Therefore, PD could only contribute modestly to the recalcitrant hypokalemia observed during the episode of pseudo-obstruction. It has been shown, however, that patients with colonic pseudo-obstruction have enhanced colonic K+ secretion. In addition, experimental studies in patients with chronic kidney disease (CKD) have demonstrated that colonic K+ excretion can be up to 3 times greater than in individuals with normal renal function. This increase may involve an upregulation of the large conductance K+ channel (maxi-K), also known as the BK channel, in the apical border of the colonocytes. We suggest that ESRD may have placed our patient at a greater risk of developing hypokalemia as his colon may have already adapted to secrete more K+. Clinicians should be aware of this extrarenal K+ wasting etiology in patients with colonic pseudo-obstruction, particularly in those with CKD where such a severe K+ deficit is not anticipated and, therefore, may inhibit more rigorous K+ replacement.
- Published
- 2017
16. Prolylcarboxypeptidase deficiency is associated with increased blood pressure, glomerular lesions, and cardiac dysfunction independent of altered circulating and cardiac angiotensin II
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Brian D. Hoit, Yashpal S. Kanwar, Michael Bader, Philipp K Haber, Ines Schadock, Daniel Batlle, Christoph Maier, Christopher A Flask, Jan Wysocki, Gregory N. Adams, Minghao Ye, Xin Yu, and Alvin H. Schmaier
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Kidney Glomerulus ,Diastole ,Blood Pressure ,Carboxypeptidases ,Nephron ,030204 cardiovascular system & hematology ,Biology ,Kidney ,Left ventricular hypertrophy ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Drug Discovery ,medicine ,Animals ,Kidney Tubules, Collecting ,Genetics (clinical) ,Renal sodium reabsorption ,Reabsorption ,Angiotensin II ,Myocardium ,medicine.disease ,Mice, Mutant Strains ,Peptide Fragments ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Blood pressure ,cardiovascular system ,Molecular Medicine ,Angiotensin I ,hormones, hormone substitutes, and hormone antagonists - Abstract
Prolylcarboxypeptidase (PRCP) is a carboxypeptidase that cleaves angiotensin II (AngII) forming Ang(1-7). The impact of genetic PRCP deficiency on AngII metabolism, blood pressure (BP), kidney histology, and cardiac phenotype was investigated in two lines of PRCP-deficient mice: KST302 derived in C57BL/6 background and GST090 derived in FVB/N background. The GST090 line had increased mean arterial pressure (MAP) (113.7 ± 2.07 vs. WT 105.0 ± 1.23 mmHg; p 0.01) and left ventricular hypertrophy (LVH) (ratio of diastolic left ventricular posterior wall dimension to left ventricular diameter 0.239 ± 0.0163 vs. WT 0.193 ± 0.0049; p 0.05). Mice in the KST302 line also had mild hypertension and LVH. Cardiac defects, increased glomerular size, and glomerular mesangial expansion were also observed. After infusion of AngII to mice in the KST302 line, both MAP and LVH increased, but the constitutive differences between the gene trap mice and controls were no longer observed. Plasma and cardiac AngII and Ang(1-7) were not significantly different between PRCP-deficient mice and controls. Thus, PRCP deficiency is associated with elevated blood pressure and cardiac alterations including LVH and cardiac defects independently of systemic or cardiac AngII and Ang(1-7). An ex vivo assay showed that recombinant PRCP, unlike recombinant ACE2, did not degrade AngII to form Ang(1-7) in plasma at pH 7.4. PRCP was localized in α-intercalated cells of the kidney collecting tubule. The low pH prevailing at this site and the acidic pH preference of PRCP suggest a role of this enzyme in regulating AngII degradation in the collecting tubule where this peptide increases sodium reabsorption and therfore BP. However, there are other potential mechanisms for increased BP in this model that need to be considered as well. PRCP converts AngII to Ang(1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP.Prolylcarboxypeptidase (PRCP) converts AngII to Ang (1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP.
- Published
- 2017
17. Urinary angiotensinogen antedates the development of stage 3 CKD in patients with type 1 diabetes mellitus
- Author
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Sheeba Habeeb Ba Aqeel, Alfred Rademaker, Daniel Batlle, Robert G. Nelson, Alejandro Sanchez, Jan Wysocki, Ionut Bebu, Mark E. Molitch, Ahmed M Khattab, Enrique Lores, Minghao Ye, Complications Trial Diabetes Control, and Xiaoyu Gao
- Subjects
Adult ,Male ,medicine.medical_specialty ,hypertension ,Adolescent ,Physiology ,Urinary system ,Urology ,Angiotensinogen ,030204 cardiovascular system & hematology ,urologic and male genital diseases ,lcsh:Physiology ,03 medical and health sciences ,chemistry.chemical_compound ,Young Adult ,0302 clinical medicine ,Physiology (medical) ,Diabetes mellitus ,Epidemiology ,medicine ,Metabolism and Regulation ,Humans ,Diabetic Nephropathies ,Renal Insufficiency, Chronic ,Original Research ,Type 1 diabetes ,Creatinine ,renin angiotensin system ,lcsh:QP1-981 ,diabetes ,business.industry ,biomarkers ,Odds ratio ,Middle Aged ,medicine.disease ,Renal Conditions, Disorders and Treatments ,3. Good health ,Blood pressure ,Diabetes Mellitus, Type 1 ,chemistry ,Case-Control Studies ,urinary angiotensinogen ,Female ,Endocrine and Metabolic Conditons, Disorders and Treatments ,business ,030217 neurology & neurosurgery ,chronic kidney disease ,Kidney disease - Abstract
We examined if urinary angiotensinogen (uAOG), a marker of intrarenal renin‐angiotensin system activity, antedates stage 3 chronic kidney disease (CKD) using samples from participants in the Diabetes Control and Complications Trial (DCCT) and later in the Epidemiology of Diabetes Intervention and Complications (EDIC) trial. In a nested case–control design, cases were matched at the outcome visit (eGFR less than 60, 21‐59 mL/min per 1.73 m2) on age, gender, and diabetes duration, with controls: eGFR (95, 75‐119, mL/min per 1.73 m2.) Additionally, in an exploratory analysis progressive renal decline (PRD), defined as eGFR loss >3.5 mL/min per 1.73m2/year, was evaluated using only data from EDIC because no progressions were observed during DCCT. At the EDIC visit, which antedated the GFR outcome visit by 2 years (range 1–7years) the median uAOG/creatinine was markedly higher in cases than in controls (13.9 vs. 3.8 ng/mg P = 0.003) whereas at the DCCT visit, which antedated the GFR outcome by 17 to 20 years it was not (2.75 vs. 3.16 ng/mg, respectively). The Odds Ratio for uAOG and CKD stage 3 development was significant after adjusting for eGFR, HbA1c, and systolic blood pressure 1.82 (1.00–3.29) but no longer significant when Albumin Excretion Ratio (AER) was included 1.21 (0.65–2.24).In the PRD analysis, uAOG/creatinine was sixfold higher in participants who experienced PRD than in those who did not (26 vs. 4.0 ng/mg, P = 0.003). The Odds Ratio for uAOG and PRD was significant after adjusting for eGFR, HbA1c, and systolic blood pressure 2.48 (1.46–4.22) but no longer significant when AER was included 1.32 (0.76–2.30). In people with type1 diabetes, a robust increase in uAOG antedates the development of stage 3 CKD but is not superior to AER in predicting this renal outcome. Increased uAOG moreover is associated with PRD, an index of progression to End Stage Kidney Disease (ESKD).
- Published
- 2019
18. Abstract 099: Novel Angiotensin-Converting-Enzyme 2 (ACE2) Truncates to Target the Kidney Renin-Angiotensin-System (RAS)
- Author
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Jan Wysocki, Chad R. Haney, Ming Zhao, Daniel Batlle, and Arndt Schulze
- Subjects
chemistry.chemical_classification ,Kidney ,medicine.medical_specialty ,medicine.disease ,Angiotensin II ,medicine.anatomical_structure ,Endocrinology ,Enzyme ,Molecular size ,chemistry ,Internal medicine ,Angiotensin-converting enzyme 2 ,Renin–angiotensin system ,Internal Medicine ,medicine ,Kidney disease - Abstract
ACE2 is an enzyme with a molecular size of more than 100 kDa which produces Angiotensin (1-7) from Angiotensin II (1-8). Attempts to target kidney RAS using ACE2 to treat kidney disease are hampered by its large molecular size which precludes its glomerular filtration and subsequent tubular uptake. We tested two ACE2 truncates (1-605 and 1-619AA) that we generated through truncation from the C terminus. ACE2 enzyme activity measured using Mca-APK-Dnp substrate was as high for each of them as native rACE2 (1-740 AA). The two truncates had an apparent MW of ~70 kDa, as expected from the amino acid sequence, as shown by western blot. For radioimaging each purified truncate was labeled with 99m Tc to study kidney uptake. After i.v. injection of Tc99m -labeled rACE2, there was kidney cortex retention for Tc99m 1-605 [8.6±0.7% of whole body (WB) radioactivity] and for Tc99m 1-619 (4.2±2.1% WB) as compared to only trace retention after injection of native Tc99m -rACE2 1-740 (1.2±0.2% WB)(figure). When infused to mice with genetic ACE2 deficiency, a single i.v. injection of 1-619 resulted in detectable ACE2 activity in urine and kidneys (23.1±4.3 and 1.96±0.73 RFU/ug prot/hr, respectively). In addition, the kidneys of ACE2-nul mice infused with 1-619 studied ex vivo formed more Ang (1-7) from exogenous Ang II than those of uninfused animals (p We conclude that our novel ACE2 truncates undergo glomerular filtration which is associated with kidney uptake of enzymatically active protein that can enhance formation of Angiotensin 1-7 from Angiotensin II (1-8). These truncates may offer a potentially useful approach to target kidney RAS overactivity to combat kidney disease.
- Published
- 2019
19. Urinary Renin in Patients and Mice With Diabetic Kidney Disease
- Author
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Minghao Ye, Jeannette Tang, Jan Wysocki, Daniel Batlle, R. A. Gomez, Maria Luisa S. Sequeira-Lopez, Mina Shirazi, Maryam Afkarian, Johannes Rein, Patricia G. Vallés, and Michael Bader
- Subjects
0301 basic medicine ,Male ,renin-angiotensin system ,Disease ,030204 cardiovascular system & hematology ,Plasma renin activity ,purl.org/becyt/ford/1 [https] ,Cohort Studies ,Mice ,Random Allocation ,0302 clinical medicine ,Reference Values ,Renin ,Diabetic Nephropathies ,Kidney ,Biopsy, Needle ,Immunohistochemistry ,Low Density Lipoprotein Receptor-Related Protein-2 ,medicine.anatomical_structure ,diabetes mellitus ,Female ,CIENCIAS NATURALES Y EXACTAS ,Glomerular Filtration Rate ,kidney ,medicine.medical_specialty ,mice ,Otras Ciencias Biológicas ,Urinary system ,Mice, Transgenic ,Urinalysis ,Sensitivity and Specificity ,Article ,Diabetes Mellitus, Experimental ,Ciencias Biológicas ,03 medical and health sciences ,Diabetes mellitus ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,medicine ,Animals ,Humans ,In patient ,RNA, Messenger ,Kidney Tubules, Collecting ,purl.org/becyt/ford/1.6 [https] ,Diabetic kidney ,business.industry ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 1 ,renin ,business - Abstract
In patients with diabetic kidney disease (DKD), plasma renin activity is usually decreased, but there is limited information on urinary renin and its origin. Urinary renin was evaluated in samples from patients with longstanding type I diabetes mellitus and mice with streptozotocin-induced diabetes mellitus. Renin-reporter mouse model (Ren1d-Cre;mT/ mG) was made diabetic with streptozotocin to examine whether the distribution of cells of the renin lineage was altered in a chronic diabetic environment. Active renin was increased in urine samples from patients with DKD (n=36), compared with those without DKD (n=38; 3.2 versus 1.3 pg/mg creatinine; P
- Published
- 2019
20. Novel Variants of Angiotensin Converting Enzyme-2 of Shorter Molecular Size to Target the Kidney Renin Angiotensin System
- Author
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Jan Wysocki, Arndt Schulze, and Daniel Batlle
- Subjects
medicine.medical_specialty ,Kidney Cortex ,030232 urology & nephrology ,ACE2 ,Renal function ,Angiotensin (1-7) ,Peptidyl-Dipeptidase A ,030204 cardiovascular system & hematology ,Biochemistry ,Article ,Renin Angiotensin System ,Renin-Angiotensin System ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,Internal medicine ,Renin–angiotensin system ,medicine ,Animals ,Molecular Biology ,Mice, Knockout ,Creatinine ,Kidney ,medicine.diagnostic_test ,Angiotensin II ,Acute Kidney Injury ,medicine.disease ,Recombinant Proteins ,3. Good health ,Mice, Inbred C57BL ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Angiotensin-converting enzyme 2 ,Angiotensin-Converting Enzyme 2 ,hormones, hormone substitutes, and hormone antagonists ,Kidney disease - Abstract
ACE2 is a monocarboxypeptidase which generates Angiotensin (1&ndash, 7) from Angiotensin II (1&ndash, 8). Attempts to target the kidney Renin Angiotensin System using native ACE2 to treat kidney disease are hampered by its large molecular size, 100 kDa, which precludes its glomerular filtration and subsequent tubular uptake. Here, we show that both urine and kidney lysates are capable of digesting native ACE2 into shorter proteins of ~60&ndash, 75 kDa and then demonstrate that they are enzymatically very active. We then truncated the native ACE2 by design from the C-terminus to generate two short recombinant (r)ACE2 variants (1-605 and 1-619AA). These two truncates have a molecular size of ~70 kDa, as expected from the amino acid sequence and as shown by Western blot. ACE2 enzyme activity, measured using a specific substrate, was higher than that of the native rACE2 (1-740 AA). When infused to mice with genetic ACE2 deficiency, a single i.v. injection of 1-619 resulted in detectable ACE2 activity in urine, whereas infusion of the native ACE2 did not. Moreover, ACE2 activity was recovered in harvested kidneys from ACE2-deficient mice infused with 1-619, but not in controls (23.1 ±, 4.3 RFU/µ, g creatinine/h and 1.96 ±, 0.73 RFU/µ, g protein/hr, respectively). In addition, the kidneys of ACE2-null mice infused with 1-619 studied ex vivo formed more Ang (1&ndash, 7) from exogenous Ang II than those infused with vehicle (AUC 8555 ±, 1933 vs. 3439 ±, 753 ng/mL, respectively, p <, 0.05) further demonstrating the functional effect of increasing kidney ACE2 activity after the infusion of our short ACE2 1-619 variant. We conclude that our novel short recombinant ACE2 variants undergo glomerular filtration, which is associated with kidney uptake of enzymatically active proteins that can enhance the formation of Ang (1&ndash, 7) from Ang II. These small ACE2 variants may offer a potentially useful approach to target kidney RAS overactivity to combat kidney injury.
- Published
- 2019
21. Hyperkalemic Forms of Renal Tubular Acidosis: Clinical and Pathophysiological Aspects
- Author
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Daniel Batlle and Jose A.L. Arruda
- Subjects
0301 basic medicine ,medicine.medical_specialty ,medicine.drug_class ,Pseudohypoaldosteronism ,030232 urology & nephrology ,Renal function ,Membrane Potentials ,Renal tubular acidosis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Distal renal tubular acidosis ,Internal medicine ,medicine ,Animals ,Humans ,Renal Insufficiency, Chronic ,Epithelial Sodium Channels ,Obstructive uropathy ,Aldosterone ,business.industry ,Sodium ,Acidosis, Renal Tubular ,Nephrons ,Hydrogen-Ion Concentration ,medicine.disease ,Hypokalemia ,Amiloride ,030104 developmental biology ,Endocrinology ,chemistry ,Nephrology ,Mineralocorticoid ,Potassium ,Hyperkalemia ,medicine.symptom ,business ,medicine.drug ,Ureteral Obstruction - Abstract
In contrast to distal type I or classic renal tubular acidosis (RTA) that is associated with hypokalemia, hyperkalemic forms of RTA also occur usually in the setting of mild-to-moderate CKD. Two pathogenic types of hyperkalemic metabolic acidosis are frequently encountered in adults with underlying CKD. One type, which corresponds to some extent to the animal model of selective aldosterone deficiency (SAD) created experimentally by adrenalectomy and glucocorticoid replacement, is manifested in humans by low plasma and urinary aldosterone levels, reduced ammonium excretion, and preserved ability to lower urine pH below 5.5. This type of hyperkalemic RTA is also referred to as type IV RTA. It should be noted that the mere deficiency of aldosterone when glomerular filtration rate is completely normal only causes a modest decline in plasma bicarbonate which emphasizes the importance of reduced glomerular filtration rate in the development of the hyperchloremic metabolic acidosis associated with SAD. Another type of hyperkalemic RTA distinctive from SAD in which plasma aldosterone is not reduced is referred to as hyperkalemic distal renal tubular acidosis because urine pH cannot be reduced despite acidemia or after provocative tests aimed at increasing sodium-dependent distal acidification such as the administration of sodium sulfate or loop diuretics with or without concurrent mineralocorticoid administration. This type of hyperkalemic RTA (also referred to as voltage-dependent distal renal tubular acidosis) has been best described in patients with obstructive uropathy and resembles the impairment in both hydrogen ion and potassium secretion that are induced experimentally by urinary tract obstruction and when sodium transport in the cortical collecting tubule is blocked by amiloride.
- Published
- 2018
22. Biological Variability of Estimated GFR and Albuminuria in CKD
- Author
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Sushrut S. Waikar, Casey M. Rebholz, Zihe Zheng, Shelley Hurwitz, Chi-yuan Hsu, Harold I. Feldman, Dawei Xie, Kathleen D. Liu, Theodore E. Mifflin, John H. Eckfeldt, Paul L. Kimmel, Ramachandran S. Vasan, Joseph V. Bonventre, Lesley A. Inker, Josef Coresh, Vasan S. Ramachandran, Joseph Bonventre, Sushrut Waikar, Venkata Sabbisetti, Jennifer Van Eyk, Dawn Chen, Qin Fu, Hermine Brunner, Vivette D’Agati, Jonathan Barasch, Casey Rebholz, Alan S. Go, Erwin Bottinger, Avelino Teixeira, Ilse Daehn, Mark Molitch, Daniel Batlle, Brad Rovin, Haifeng Wu, Andrew S. Levey, Meredith Foster, Kathleen Liu, Jon Klein, Michael Mauer, Paola Fioretto, Gary Nelsestuen, Amy Karger, Shawn Ballard, Krista Whitehead, Phyllis Gimotty, Haochang Shou, Xiaoming Zhang, Kellie Ryan, Tom Greene, Robert G. Nelson, and John W. Kusek
- Subjects
Male ,urinary albumin-creatinine ratio (UACR) ,030232 urology & nephrology ,Urine ,030204 cardiovascular system & hematology ,Severity of Illness Index ,filtration marker ,intraindividual variation ,chemistry.chemical_compound ,0302 clinical medicine ,cystatin C ,Albuminuria ,beta trace protein (BTP) ,biological variability ,biomarker ,clinically meaningful differences ,coefficient of variation (CV) ,estimated glomerular filtration rate (eGFR) ,kidney function ,laboratory measurement ,reproducibility ,serum creatinine ,β ,2 ,microglobulin (B2M) ,biology ,Middle Aged ,Prognosis ,Lipocalins ,Intramolecular Oxidoreductases ,Nephrology ,Creatinine ,Biomarker (medicine) ,Female ,medicine.symptom ,Glomerular Filtration Rate ,Adult ,medicine.medical_specialty ,Urinary system ,Urology ,Renal function ,Urinalysis ,Article ,03 medical and health sciences ,Predictive Value of Tests ,medicine ,Humans ,Renal Insufficiency, Chronic ,Aged ,business.industry ,medicine.disease ,Cross-Sectional Studies ,Cystatin C ,chemistry ,biology.protein ,beta 2-Microglobulin ,business ,Biomarkers ,Blood Chemical Analysis ,Kidney disease - Abstract
RATIONALE & OBJECTIVE: Determining whether a change in estimated glomerular filtration rate (eGFR) or albuminuria is clinically significant requires knowledge of short-term within-person variability of the measurements, which few studies have addressed in the setting of chronic kidney disease. STUDY DESIGN: Cross-sectional study with multiple collections over less than 4 weeks. SETTING & PARTICIPANTS: Clinically stable outpatients with chronic kidney disease (N = 50; mean age, 56.8 years; median eGFR, 40 mL/min/1.73 m(2); median urinary albumin-creatinine ratio (UACR), 173 mg/g). EXPOSURE: Repeat measurements from serially collected samples across 3 study visits. OUTCOMES: Measurements of urine albumin concentration (UAC), UACR, and plasma creatinine, cystatin C, β(2)-microglobulin (B2M), and beta trace protein (BTP). ANALYTICAL APPROACH: We calculated within-person coefficients of variation (CV(w)) values and corresponding reference change positive and negative (RCV(pos) and RCV(neg)) values using log-transformed measurements. RESULTS: Median CV(w) (RCV(pos); RCV(neg)) values of filtration markers were 5.4% (+16%; –14%) for serum creatinine, 4.1% (+12%; –11%) for cystatin C, 7.4% (+23%; –18%) for BTP, and 5.6% (+17%; –14%) for B2M. Results for albuminuria were 33.2% (+145%; –59%) for first-morning UAC, 50.6% (+276%; –73%) for random spot UAC, 32.5% (+141%; –58%) for first-morning UACR, and 29.7% (124%; –55%) for random spot UACR. CV(w) values for filtration markers were comparable across the range of baseline eGFRs. CV(w) values for UAC and UACR were comparable across the range of baseline albuminuria values. LIMITATIONS: Small sample size limits the ability to detect differences in variability across markers. Participants were recruited and followed up in a clinical and not research setting, so some preanalytical factors could not be controlled. CONCLUSIONS: eGFR markers appear to have relatively low short-term within-person variability, whereas variability in albuminuria appears to be high, making it difficult to distinguish random variability from meaningful biologic changes.
- Published
- 2018
23. Plasma and Kidney Angiotensin Peptides: Importance of the Aminopeptidase A/Angiotensin III Axis
- Author
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Jan Wysocki, Daniel Batlle, and Minghao Ye
- Subjects
Male ,medicine.medical_specialty ,Angiotensins ,Angiotensin III ,Peptide ,Peptidyl-Dipeptidase A ,Glutamyl Aminopeptidase ,Kidney ,Aminopeptidase ,Renin-Angiotensin System ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,medicine ,Animals ,Mice, Knockout ,chemistry.chemical_classification ,business.industry ,Angiotensin II ,Mice, Inbred C57BL ,Enzyme ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Angiotensin-converting enzyme 2 ,cardiovascular system ,Female ,Original Article ,Angiotensin-Converting Enzyme 2 ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Background The renin-angiotensin system is a complex regulatory hormonal network with a main biological peptide and therapeutic target, angiotensin (Ang) II (1-8). There are other potentially important Ang peptides that have not been well evaluated. Methods Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for concurrent evaluation of multiple Angs downstream of Ang I (1-10) and Ang II (1-8) in kidney and plasma from wild-type (WT) mice. Angiotensin converting enzyme 2 knockout (ACE2KO) was also used as a way to examine the Angs profile in the absence of ACE2, an enzyme that cleaves both Ang I (1-10) and Ang II (1-8). Results In plasma from both WT and ACE2KO, levels of Ang I (1-10), Ang III (2-8), and Ang (2-10) were the highest of all the renin-angiotensin system (RAS) peptides. The latter two peptides are products of aminopeptidase A cleavage of Ang II (1-8) and Ang I (1-10), respectively. In contrast, plasma levels of Ang II (1-8), and Ang (1-7), the product of Ang II (1-8) cleavage by ACE2, were low. In kidney from both WT and ACE2KO, Ang II (1-8) levels were high as compared to plasma levels. In the ACE2KO mice, a significant increase in either Ang II (1-8) or a decrease in Ang (1-7) was not observed in plasma or in the kidney. Conclusion RAS-focused peptidomic approach revealed major differences in Ang peptides between mouse plasma and kidney. These Ang peptide profiles show the dominance of the aminopeptidase A/Ang (2-10) and aminopeptidase A/Ang III (2-8) pathways in the metabolism of Ang I (1-10) and Ang II (1-8) over the ACE2/Ang (1-7) axis. Ang III (2-8) and other peptides formed from aminopeptidase A cleavage may be important therapeutic RAS targets.
- Published
- 2015
24. Inherited Proximal and Distal Renal Tubular Acidosis
- Author
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Jesus Moran-Farias, Daniel Batlle, AS Singh, and Patricia G. Vallés
- Subjects
03 medical and health sciences ,Pathology ,medicine.medical_specialty ,0302 clinical medicine ,Distal renal tubular acidosis ,business.industry ,030232 urology & nephrology ,Medicine ,030204 cardiovascular system & hematology ,business ,medicine.disease - Abstract
Acid-base homeostasis by the kidney is maintained through proximal tubular reclamation of filtered bicarbonate and the excretion of the daily acid load by collecting duct type A intercalated cells. The impairment of either process results in renal tubular acidosis (RTA), a group of disorders characterized by a reduced net acid excretion and a persistent hyperchloremic, non–anion gap metabolic acidosis. The primary or hereditary forms of proximal (pRTA) and distal renal tubular acidosis (dRTA) have received increased attention because of advances in the understanding of the molecular mechanism, whereby mutations in the main proteins involved in acid-base transport result in either reduced bicarbonate reabsorption or reduced H+ secretion and impaired acid excretion. dRTA is characterized by reduced net acid excretion and an inability to lower urine pH despite severe acidemia (but minimal HCO3– wastage). pRTA (type 2), by contrast, is characterized by marked HCO3– wastage but preserved ability to lower urine pH when plasma HCO3– (and therefore filtered HCO3–) is below a certain threshold. In children with dRTA, growth retardation caused by chronic metabolic acidosis is the key manifestation but is fully reversible with appropriate alkali therapy if initiated early in life. A striking manifestation of many patients with dRTA is the development of severe hypokalemia that may cause muscle paralysis. In this review, we discuss these types of hereditary RTA and the mechanisms involved in the genesis of these inherited tubular disorders. This review contains 5 figures, 1 table, and 103 references. Key words: Proximal renal tubular acidosis (pRTA), Distal renal tubular acidosis (dRTA), Hyperchloremic, non–anion gap metabolic acidosis, Hypokalemia, Fractional HCO3– excretion, Urinary gap, Fanconi Syndrome.ATP6V1B1 and ATP6V0A4 gene mutations . Intercalated cells
- Published
- 2017
25. Metabolic Acidosis or Respiratory Alkalosis? Evaluation of a Low Plasma Bicarbonate Using the Urine Anion Gap
- Author
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Jamie Chin-Theodorou, Bryan M. Tucker, and Daniel Batlle
- Subjects
medicine.medical_specialty ,Bicarbonate ,Urinary system ,030232 urology & nephrology ,Water-Electrolyte Imbalance ,Urine ,Gastroenterology ,Article ,Excretion ,Diagnosis, Differential ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Hyperventilation ,030212 general & internal medicine ,Intensive care medicine ,Aged, 80 and over ,business.industry ,Disease Management ,Metabolic acidosis ,Hydrogen-Ion Concentration ,medicine.disease ,Stroke ,chemistry ,Nephrology ,Respiratory alkalosis ,Urine anion gap ,Female ,Net acid excretion ,business ,Acidosis ,Alkalosis, Respiratory - Abstract
Hypobicarbonatemia, or a reduced bicarbonate concentration in plasma, is a finding seen in 3 acid-base disorders: metabolic acidosis, chronic respiratory alkalosis and mixed metabolic acidosis and chronic respiratory alkalosis. Hypobicarbonatemia due to chronic respiratory alkalosis is often misdiagnosed as a metabolic acidosis and mistreated with the administration of alkali therapy. Proper diagnosis of the cause of hypobicarbonatemia requires integration of the laboratory values, arterial blood gas, and clinical history. The information derived from the urinary response to the prevailing acid-base disorder is useful to arrive at the correct diagnosis. We discuss the use of urine anion gap, as a surrogate marker of urine ammonium excretion, in the evaluation of a patient with low plasma bicarbonate concentration to differentiate between metabolic acidosis and chronic respiratory alkalosis. The interpretation and limitations of urine acid-base indexes at bedside (urine pH, urine bicarbonate, and urine anion gap) to evaluate urine acidification are discussed.
- Published
- 2016
26. Renal Tubular Acidosis and the Nephrology Teaching Paradigm
- Author
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Jose A.L. Arruda and Daniel Batlle
- Subjects
Nephrology ,medicine.medical_specialty ,Career Choice ,business.industry ,Teaching ,MEDLINE ,Acidosis, Renal Tubular ,medicine.disease ,Renal tubular acidosis ,Internal medicine ,medicine ,Humans ,medicine.symptom ,business ,Intensive care medicine ,Career choice ,Acidosis - Published
- 2018
27. Apelinergic system in the kidney: implications for diabetic kidney disease
- Author
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Tilman Müller, Michael Bader, Anastasia Z. Kalea, Daniel Batlle, Minghao Ye, Ivy Hsieh, Jan Wysocki, Alonso Marquez, and Syed K. Haque
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,medicine.medical_specialty ,Physiology ,Apoptosis ,Kidney ,Cell Line ,Podocyte ,Mice ,03 medical and health sciences ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Diabetic Nephropathies ,Extracellular Signal-Regulated MAP Kinases ,Receptor ,Protein kinase B ,Original Research ,Caspase 3 ,Podocytes ,Chemistry ,Ribosomal Protein S6 Kinases, 70-kDa ,Angiotensin II ,Renal Conditions, Disorders and Treatments ,diabetic kidney disease ,3. Good health ,Apelin ,Mice, Inbred C57BL ,Cellular and Molecular Physiology ,Endothelial stem cell ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,apelin ,APJ ,Intercellular Signaling Peptides and Proteins ,Female ,Endocrine and Metabolic Conditons, Disorders and Treatments ,Proto-Oncogene Proteins c-akt - Abstract
The bioactive peptides of the apelinergic system and its receptor APJ have been shown to play a protective role in experimental cardiovascular and diabetic kidney disease (DKD). Mechanisms of this renoprotective effect remain to be elucidated. In this study, we examined the localization of APJ within the normal kidney and its kidney expression in the db/db model of DKD. The effect of hyperglycemia and angiotensin II on APJ was examined in cultured podocytes. In the glomerulus, APJ colocalized with podocyte but not endothelial cell markers. In podocytes stimulated with Pyr 1Apelin‐13, a change in the phosphorylation status of the signaling proteins, AKT, ERK, and p70S6K, was observed with an increase 15 min after stimulation. Apelin‐13 decreased activity of Caspase‐3 in podocytes after high glucose treatment reflecting an antiapoptotic effect of APJ stimulation. In podocytes, APJ mRNA was downregulated in high glucose, when compared to normal glucose conditions and exposure to angiotensin II led to a further significant decrease in APJ mRNA. APJ and preproapelin mRNA levels in kidneys from db/db mice were markedly decreased along with decreased tubular APJ protein by western blotting and immunostaining when compared to db/m controls. In conclusion, the apelinergic system is decreased in kidneys from db/db mice. Within the glomerulus, APJ is mainly localized in podocytes and in this cell type its activation by Apelin‐13 abolishes the proapoptotic effect of high glucose, suggesting a potential therapeutic role of apelin and emerging agonists with extended half‐life for therapy of DKD.
- Published
- 2018
28. Regulation of urinary ACE2 in diabetic mice
- Author
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Laura Garcia-Halpin, Jan Wysocki, Daniel Batlle, Kurt Sowers, Kevin D. Burns, Minghao Ye, and Christoph Maier
- Subjects
Male ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,Urinary system ,Urology ,Urine ,Peptidyl-Dipeptidase A ,Nephropathy ,Internal medicine ,medicine ,Animals ,Diabetic Nephropathies ,Kidney ,business.industry ,Angiotensin II ,Insulin ,Captopril ,Articles ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,Female ,Telmisartan ,business ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Angiotensin-converting enzyme-2 (ACE2) enhances the degradation of ANG II and its expression is altered in diabetic kidneys, but the regulation of this enzyme in the urine is unknown. Urinary ACE2 was studied in the db/db model of type 2 diabetes and stretozotocin (STZ)-induced type 1 diabetes during several physiological and pharmacological interventions. ACE2 activity in db/db mice was increased in the serum and to a much greater extent in the urine compared with db/m controls. Neither a specific ANG II blocker, telmisartan, nor an ACE inhibitor, captopril, altered the levels of urinary ACE2 in db/db or db/m control mice. High-salt diet (8%) increased whereas low-salt diet (0.1%) decreased urinary ACE2 activity in the urine of db/db mice. In STZ mice, urinary ACE2 was also increased, and insulin decreased it partly but significantly after several weeks of administration. The increase in urinary ACE2 activity in db/db mice reflected an increase in enzymatically active protein with two bands identified of molecular size at 110 and 75 kDa and was associated with an increase in kidney cortex ACE2 protein at 110 kDa but not at 75 kDa. ACE2 activity was increased in isolated tubular preparations but not in glomeruli from db/db mice. Administration of soluble recombinant ACE2 to db/m and db/db mice resulted in a marked increase in serum ACE2 activity, but no gain in ACE2 activity was detectable in the urine, further demonstrating that urinary ACE2 is of kidney origin. Increased urinary ACE2 was associated with more efficient degradation of exogenous ANG II (10−9 M) in urine from db/db compared with that from db/m mice. Urinary ACE2 could be a potential biomarker of increased metabolism of ANG II in diabetic kidney disease.
- Published
- 2013
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29. Predominance of AT1 Blockade Over Mas–Mediated Angiotensin-(1–7) Mechanisms in the Regulation of Blood Pressure and Renin–Angiotensin System in mRen2.Lewis Rats
- Author
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Daniel Batlle, Sarfaraz Ahmad, Jasmina Varagic, Jan Wysocki, Carlos M. Ferrario, Norihito Moniwa, K. Bridget Brosnihan, and Jessica L VonCannon
- Subjects
medicine.medical_specialty ,Angiotensin II receptor type 1 ,Aldosterone ,biology ,business.industry ,Angiotensin-converting enzyme ,Pharmacology ,Angiotensin II ,Plasma renin activity ,chemistry.chemical_compound ,Endocrinology ,Mechanism of action ,chemistry ,Internal medicine ,Renin–angiotensin system ,cardiovascular system ,Internal Medicine ,medicine ,biology.protein ,Original Article ,medicine.symptom ,Olmesartan ,business ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Although the mechanism of action of angiotensin II (Ang II) receptor (AT1-R) blockers (ARBs) is well established, data suggest that their antihypertensive effects may be limited in part by the overriding effect of compensatory increases in plasma renin activity (PRA) and Ang II.1 Contrasting with the mechanism of action of other ARBs, Agata et al. 2 reported that the antihypertensive and cardiac antihypertrophic actions of 4-week olmesartan medoxomil administration were not associated with the expected increases in circulating Ang II in adult stroke-prone spontaneously hypertensive rats (SHR). Agata et al. 2 speculated that the failure of plasma Ang II to rise in response to blockade of AT1-R could be explained by angiotensin-(1–7) [Ang-(1–7)]–induced inhibition of angiotensin converting enzyme (ACE) activity, given previous observations of increased Ang-(1–7) after treatment with ARBs,3,4 as well as an inhibitory role of the heptapeptide at the C- and N-terminus catalytic activity of the enzyme.5,6 The intriguing possibility of a differential effect of olmesartan on plasma Ang II levels compared with other ARBs was also consistent with 2 other studies in human essential hypertensive subjects in whom prolonged therapy with olmesartan was accompanied by either decreases or no changes in plasma Ang II levels.7,8 The effects of olmesartan on Ang II levels may also result from increased conversion of the octapeptide to Ang-(1–7) because ACE2 has been shown to be upregulated by AT1-R blockade.4,9,10 This has been demonstrated in vivo by recent work showing that the infusion of soluble human recombinant ACE2 efficiently lowered plasma Ang II while increasing Ang-(1–7).10 Furthermore, in isolated cardiac myocytes, ACE2 messenger RNA expression and activity were not affected by Ang-(1–7); however, the inhibitory effects of Ang II on ACE2 were blocked by Ang-(1–7).11 The heptapeptide modulatory effect was prevented by the Ang-(1–7) mas receptor antagonist [D-ALA7]-Ang-(1–7) (A-779), indicating that the Ang-(1–7) response was mediated by a specific Ang-(1–7) receptor. A-779 is a selective blocker of the mas receptor that has been identified to mediate vasodilatory, antitrophic, and antiproliferative effects of Ang-(1–7).12–14 The long-term effects of Ang-(1–7) antagonism in the presence of concomitant Ang II receptor blockade have not been determined. With this in mind, we investigated the Ang-(1–7)–mediated effects of olmesartan on blood pressure, plasma, renal, and cardiac Ang II as well as ACE2 in mRen2.Lewis congenic hypertensive rats. This monogenetic hypertensive rat strain was developed in our laboratory through a backcross of the hypertensive (mRen2)27 transgenic rats with normotensive Lewis rats. The aim of this backcross was to offset the heterogeneity of the parent strain that contributed to the genetic variability found within the original transgenic strain.15,16 Because the malignant phase of hypertension is not observed in mRen2.Lewis rats, the longer life span of this experimental model provides a better opportunity to investigate the function and regulation of tissue renin–angiotensin system (RAS) and its contribution to the etiology of hypertension and target organ damage.
- Published
- 2013
30. Proximal renal tubular acidosis: a not so rare disorder of multiple etiologies
- Author
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Syed K. Haque, Gema Ariceta, and Daniel Batlle
- Subjects
medicine.medical_specialty ,viruses ,Bicarbonate ,030232 urology & nephrology ,Cutting-Edge Renal Science ,Renal tubular acidosis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Carbonic anhydrase ,Internal medicine ,medicine ,Animals ,Humans ,proximal RTA ,030304 developmental biology ,Acidosis ,0303 health sciences ,Transplantation ,biology ,Reabsorption ,business.industry ,Fanconi syndrome ,Bicarbonate transport ,Acidosis, Renal Tubular ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,drug-induced pRTA ,3. Good health ,Endocrinology ,chemistry ,Nephrology ,Mutation ,biology.protein ,Reviews - Basic Science and Translational Nephrology ,hereditary pRTA ,renal tubular acidosis ,medicine.symptom ,business ,Proximal renal tubular acidosis - Abstract
Proximal renal tubular acidosis (RTA) (Type II RTA) is characterized by a defect in the ability to reabsorb HCO(3) in the proximal tubule. This is usually manifested as bicarbonate wastage in the urine reflecting that the defect in proximal tubular transport is severe enough that the capacity for bicarbonate reabsorption in the thick ascending limb of Henle's loop and more distal nephron segments is overwhelmed. More subtle defects in proximal bicarbonate transport likely go clinically unrecognized owing to compensatory reabsorption of bicarbonate distally. Inherited proximal RTA is more commonly autosomal recessive and has been associated with mutations in the basolateral sodium-bicarbonate cotransporter (NBCe1). Mutations in this transporter lead to reduced activity and/or trafficking, thus disrupting the normal bicarbonate reabsorption process of the proximal tubules. As an isolated defect for bicarbonate transport, proximal RTA is rare and is more often associated with the Fanconi syndrome characterized by urinary wastage of solutes like phosphate, uric acid, glucose, amino acids, low-molecular-weight proteins as well as bicarbonate. A vast array of rare tubular disorders may cause proximal RTA but most commonly it is induced by drugs. With the exception of carbonic anhydrase inhibitors which cause isolated proximal RTA, drug-induced proximal RTA is associated with Fanconi syndrome. Drugs that have been recently recognized to cause severe proximal RTA with Fanconi syndrome include ifosfamide, valproic acid and various antiretrovirals such as Tenofovir particularly when given to human immunodeficiency virus patients receiving concomitantly protease inhibitors such as ritonavir or reverse transcriptase inhibitors such as didanosine.
- Published
- 2012
31. Abstract P314: Urinary Renin and Kidney RAS Activation in Mice with Diabetic Kidney Disease
- Author
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Minghao Ye, Mariam Afkarian, Jan Wysocki, Johannes Rein, Patricia G. Vallés, and Daniel Batlle
- Subjects
medicine.medical_specialty ,Kidney ,Endocrinology ,medicine.anatomical_structure ,Diabetic kidney ,business.industry ,Internal medicine ,Urinary system ,Renin–angiotensin system ,Internal Medicine ,Medicine ,Disease ,business - Abstract
RAS is overactive in kidneys from patients with diabetic nephropathy (DN), but circulating plasma renin activity (PRA) is usually low. This is known as the renin-paradox. We evaluated juxtaglomerular (JGA), tubular and urinary renin, as a potential source of local RAS activation, to gain some understanding of this paradox. Mice with STZ induced diabetes were used which had mild albuminuria and glomerular mesangial expansion consistent with early DN. Renin expression in the JGA and in the collecting tubule (CT) was evaluated by immunohistochemistry. IF was used to localize renin within CT cells. Proximal tubular renin was evaluated by RT-real time PCR in microdissected proximal tubules (PT). Urinary renin and Ang II were measured by ELISA. Urinary Ang II was increased (37.8±11.4 vs. 99.0±21.6 pg/mg creat, p We conclude that in DN renin expression in the JGA, the physiologic site of renin secretion into the circulation is suppressed, whereas in the CT it is increased. Activation of the kidney RAS, as inferred from increased urinary Ang II, likely occurs as a result of renin of tubular origin rather than from JGA renin. Since PT renin is not increased, the CT may provide the source of tubular renin for RAS activation.
- Published
- 2016
32. Urinary Angiotensinogen: A Promising Biomarker of AKI Progression in Acute Decompensated Heart Failure: What Does It Mean?
- Author
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Daniel Batlle and Jan Wysocki
- Subjects
Male ,Time Factors ,Acute decompensated heart failure ,Epidemiology ,030232 urology & nephrology ,Angiotensinogen ,030204 cardiovascular system & hematology ,urologic and male genital diseases ,Critical Care and Intensive Care Medicine ,chemistry.chemical_compound ,0302 clinical medicine ,Hepatitis A Virus Cellular Receptor 1 ,Prospective Studies ,Aged, 80 and over ,Kidney ,Interleukin-18 ,Acute Kidney Injury ,Middle Aged ,female genital diseases and pregnancy complications ,Lipocalins ,medicine.anatomical_structure ,Nephrology ,Creatinine ,Acute Disease ,Cardiology ,Disease Progression ,Biomarker (medicine) ,Female ,medicine.medical_specialty ,Urinary system ,Clinical marker ,Early detection ,Risk Assessment ,03 medical and health sciences ,Lipocalin-2 ,Internal medicine ,Proto-Oncogene Proteins ,medicine ,Albuminuria ,Humans ,Aged ,Heart Failure ,Transplantation ,Cardio-Renal Syndrome ,urogenital system ,business.industry ,Editorials ,Original Articles ,medicine.disease ,chemistry ,Heart failure ,business ,Biomarkers ,Acute-Phase Proteins - Abstract
A major challenge in early treatment of acute cardiorenal syndrome (CRS) is the lack of predictors for progression of AKI. We aim to investigate the utility of urinary angiotensinogen and other renal injury biomarkers in predicting AKI progression in CRS.In this prospective, multicenter study, we screened 732 adults who admitted for acute decompensated heart failure from September 2011 to December 2014, and evaluated whether renal injury biomarkers measured at time of AKI diagnosis can predict worsening of AKI. In 213 patients who developed Kidney Disease Improving Global Outcomes stage 1 or 2 AKI, six renal injury biomarkers, including urinary angiotensinogen (uAGT), urinary neutrophil gelatinase-associated lipocalin (uNGAL), plasma neutrophil gelatinase-associated lipocalin, urinary IL-18 (uIL-18), urinary kidney injury molecule-1, and urinary albumin-to-creatinine ratio, were measured at time of AKI diagnosis. The primary outcome was AKI progression defined by worsening of AKI stage (50 patients). The secondary outcome was AKI progression with subsequent death (18 patients).After multivariable adjustment, the highest tertile of three urinary biomarkers remained associated with AKI progression compared with the lowest tertile: uAGT (odds ratio [OR], 10.8; 95% confidence interval [95% CI], 3.4 to 34.7), uNGAL (OR, 4.7; 95% CI, 1.7 to 13.4), and uIL-18 (OR, 3.6; 95% CI, 1.4 to 9.5). uAGT was the best predictor for both primary and secondary outcomes with area under the receiver operating curve of 0.78 and 0.85. These three biomarkers improved risk reclassification compared with the clinical model alone, with uAGT performing the best (category-free net reclassification improvement for primary and secondary outcomes of 0.76 [95% CI, 0.46 to 1.06] and 0.93 [95% CI, 0.50 to 1.36]; P0.001). Excellent performance of uAGT was further confirmed with bootstrap internal validation.uAGT, uNGAL, and uIL-18 measured at time of AKI diagnosis improved risk stratification and identified CRS patients at highest risk of adverse outcomes.
- Published
- 2016
33. The Use of Bedside Urinary Parameters in the Evaluation of Metabolic Acidosis
- Author
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Daniel Batlle, Nitin Relia, and Khurram Saleem
- Subjects
medicine.medical_specialty ,Chemistry ,Urinary system ,Bicarbonate ,030232 urology & nephrology ,Metabolic acidosis ,Urine ,030204 cardiovascular system & hematology ,medicine.disease ,pCO2 ,Excretion ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Internal medicine ,medicine ,Urine anion gap ,Ammonium ,Intensive care medicine - Abstract
The evaluation of metabolic acidosis requires an assessment of the kidneys response in terms of acid excretion and bicarbonate excretion. Because of bedside measurements of ammonium excretion and bicarbonate are often not available because clinical laboratories do not perform these tests routinely, surrogates like the urine anion gap were developed as an indirect measure of the ammonium excretion. Bicarbonate can be calculated based on the urine pH and PCO2 of the urine. The significance and limitations of the urine pH as an index of collecting tubule H+ secretion as discussed. Moreover, the use of provocative tests such as the furosemide test as a tool to assess sodium dependent collecting tubule H+ secretion is also discussed.
- Published
- 2016
34. Pathophysiologic Approach to Metabolic Acidosis
- Author
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Daniel Batlle and Nitin Relia
- Subjects
medicine.medical_specialty ,Endocrinology ,Chemistry ,Clinical history ,Internal medicine ,medicine ,Anion gap ,Metabolic acidosis ,Hyperchloremic metabolic acidosis ,medicine.disease ,Plasma bicarbonate ,Pathophysiology ,Serum bicarbonate - Abstract
Evaluation of a presumed metabolic acidosis begins with the clinical history and assessment of potential causes of decreased plasma bicarbonate levels. Calculation of the plasma anion gap is the initial step in the evaluation of metabolic acidosis. The type of metabolic acidosis present can be initially approached by assessing whether plasma anion gap (AG) is normal or elevated and helps differentiate hyperchloremic metabolic acidosis (normal AG) from high AG metabolic acidosis. The relationship between the increase in the anion gap above normal ΔAG and the decrease in serum bicarbonate concentration below normal ΔHCO3 helps uncover the presence of a mixed acid–base disorders. Deviations from the presumed 1:1 ratio in this relationship that is present in a high AG metabolic acidosis can be used to diagnose complex mixed acid–base disorders.
- Published
- 2016
35. Podocyte-specific overexpression of human angiotensin-converting enzyme 2 attenuates diabetic nephropathy in mice
- Author
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Jan Wysocki, Chris R. J. Kennedy, Alex Gutsol, Marc Dilauro, Joseph Zimpelmann, Renisha Nadarajah, Fengxia Xiao, Rosangela Milagres, Daniel Batlle, and Kevin D. Burns
- Subjects
medicine.medical_specialty ,podocyte ,Transgene ,ACE2 ,030204 cardiovascular system & hematology ,albuminuria ,Podocyte ,Nephrin ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Internal medicine ,medicine ,030304 developmental biology ,0303 health sciences ,diabetes ,biology ,business.industry ,apoptosis ,angiotensin ,medicine.disease ,Streptozotocin ,Angiotensin II ,3. Good health ,medicine.anatomical_structure ,Endocrinology ,Nephrology ,Angiotensin-converting enzyme 2 ,biology.protein ,business ,medicine.drug - Abstract
Angiotensin-converting enzyme 2 (ACE2) degrades angiotensin II to angiotensin-(1–7) and is expressed in podocytes. Here we overexpressed ACE2 in podocytes in experimental diabetic nephropathy using transgenic methods where a nephrin promoter drove the expression of human ACE2. Glomeruli from these mice had significantly increased mRNA, protein, and activity of ACE2 compared to wild-type mice. Male mice were treated with streptozotocin to induce diabetes. After 16 weeks, there was no significant difference in plasma glucose levels between wild-type and transgenic diabetic mice. Urinary albumin was significantly increased in wild-type diabetic mice at 4 weeks, whereas albuminuria in transgenic diabetic mice did not differ from wild-type nondiabetic mice. However, this effect was transient and by 16 weeks both transgenic and nontransgenic diabetic mice had similar rates of proteinuria. Compared to wild-type diabetic mice, transgenic diabetic mice had an attenuated increase in mesangial area, decreased glomerular area, and a blunted decrease in nephrin expression. Podocyte numbers decreased in wild-type diabetic mice at 16 weeks, but were unaffected in transgenic diabetic mice. At 8 weeks, kidney cortical expression of transforming growth factor-β1 was significantly inhibited in transgenic diabetic mice as compared to wild-type diabetic mice. Thus, the podocyte-specific overexpression of human ACE2 transiently attenuates the development of diabetic nephropathy.
- Published
- 2012
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36. Angiotensin-converting enzyme 2: enhancing the degradation of angiotensin II as a potential therapy for diabetic nephropathy
- Author
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Jan Wysocki, María José Soler, Keerthi Ranganath, and Daniel Batlle
- Subjects
medicine.medical_specialty ,Angiotensin receptor ,Angiotensin-Converting Enzyme Inhibitors ,Peptidyl-Dipeptidase A ,030204 cardiovascular system & hematology ,Kidney ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Renin–angiotensin system ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,renin–angiotensin system ,030304 developmental biology ,0303 health sciences ,Angiotensin II receptor type 1 ,biology ,urogenital system ,business.industry ,Angiotensin II ,diabetic nephropathy ,Angiotensin-converting enzyme ,angiotensin ,medicine.disease ,Peptide Fragments ,Recombinant Proteins ,3. Good health ,Endocrinology ,Nephrology ,Angiotensin-converting enzyme 2 ,Albuminuria ,biology.protein ,Angiotensin-Converting Enzyme 2 ,Angiotensin I ,medicine.symptom ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Angiotensin-converting enzyme 2 (ACE2) is a monocarboxypeptidase that degrades angiotensin II with high efficiency leading to the formation of angiotensin-(1–7). ACE2 within the kidneys is largely localized in tubular epithelial cells and in glomerular epithelial cells. Decreased glomerular expression of this enzyme coupled with increased expression of ACE has been described in diabetic kidney disease, both in mice and humans with type 2 diabetes. Moreover, both ACE2 genetic ablation and pharmacological ACE2 inhibition have been shown to increase albuminuria and promote glomerular injury. Studies using recombinant ACE2 have shown the ability of ACE2 to rapidly metabolize Ang II in vivo and form the basis for future studies to examine the potential of ACE2 amplification in the therapy of diabetic kidney disease and cardiovascular disease.
- Published
- 2012
37. Snapshot Hemodynamics and Clinical Outcomes in Hypertension: Precision in the Measurements Is Key
- Author
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Jay A Pandit and Daniel Batlle
- Subjects
medicine.medical_specialty ,Ambulatory blood pressure ,Hemodynamics ,Guidelines as Topic ,030204 cardiovascular system & hematology ,Prehypertension ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Internal Medicine ,medicine ,Humans ,030212 general & internal medicine ,Adverse effect ,business.industry ,Disease Management ,Blood Pressure Determination ,Surgery ,Clinical trial ,Blood pressure ,Sprint ,Hypertension ,Cardiology ,Aortic pressure ,business - Abstract
The recent publication of the Systolic Blood Pressure Intervention Trial (SPRINT) inks the latest chapter in the evolving history of blood pressure management.1 The trial randomized 9361 nondiabetic hypertensive patients aged >50 years with at least one cardiovascular risk factor (not including stroke) to intensive or standard control (systolic blood pressure
- Published
- 2015
38. MP490FORMATION OF ANG(1−7) FROM ANGII(1−8) IS LARGELY INDEPENDENT OF ACE2 AND PRCP
- Author
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Peter D Serfozo, Jan Wysocki, Pan Liu, Minghao Ye, Jing Jin, Tilman Müller, and Daniel Batlle
- Subjects
Transplantation ,medicine.medical_specialty ,Endocrinology ,Nephrology ,business.industry ,Internal medicine ,Medicine ,business - Published
- 2017
39. Improving CKD Therapies and Care
- Author
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Maria E. Ferris, Daniel Batlle, Michael F. Flessner, Srinivasan Beddhu, Harold I. Feldman, Lawrence J. Fine, Robert A. Star, John T. Daugirdas, Frederick J. Kaskel, Barry I. Freedman, and Paul L. Kimmel
- Subjects
Gerontology ,Nephrology ,medicine.medical_specialty ,Biomedical Research ,Quality management ,Epidemiology ,Alternative medicine ,Psychological intervention ,Translational research ,Disease ,Critical Care and Intensive Care Medicine ,Risk Factors ,Internal medicine ,Intervention (counseling) ,medicine ,Animals ,Humans ,Organizational Objectives ,Renal Insufficiency, Chronic ,Quality Indicators, Health Care ,Transplantation ,Health Priorities ,business.industry ,Special Features ,Quality Improvement ,Treatment Outcome ,Observational study ,business - Abstract
The Kidney Research National Dialogue, supported by the National Institute of Diabetes and Digestive and Kidney Diseases, asked the research and clinical communities to formulate and prioritize research objectives that would improve our understanding of kidney function and diseases. This commentary outlines the high-priority research objectives for CKD. The goal of these research objectives is to enhance knowledge to improve outcomes in people with CKD. Basic and translational research, longitudinal observations, and epidemiologic studies can each point to targets for intervention. Future interventions must be informed by data from well designed, large representative observational studies that include collection of genetic and phenotypic data as well as biospecimens. Interaction of genetic and environmental factors must be part of the analysis, including the influence of diet, comorbid conditions, and medication. The focus should be not only on slowing or preventing progression of CKD, but also on regression of disease to the greatest extent possible.
- Published
- 2014
40. Targeting the Degradation of Angiotensin II With Recombinant Angiotensin-Converting Enzyme 2
- Author
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Minghao Ye, Manfred Schuster, Jan Wysocki, Carlos M. Ferrario, K. Bridget Brosnihan, Clara Barrios, Karla Evora, Josef M. Penninger, Francisco R. González-Pacheco, Eva Rodríguez, Daniel Batlle, and Hans Loibner
- Subjects
Kidney ,medicine.medical_specialty ,business.industry ,Kidney metabolism ,Peptide hormone ,Angiotensin II ,Dose–response relationship ,medicine.anatomical_structure ,Endocrinology ,Blood pressure ,Internal medicine ,Renin–angiotensin system ,Angiotensin-converting enzyme 2 ,cardiovascular system ,Internal Medicine ,medicine ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Angiotensin (Ang)-converting enzyme 2 (ACE2) cleaves Ang II to form Ang-(1-7). Here we examined whether soluble human recombinant ACE2 (rACE2) can efficiently lower Ang II and increase Ang-(1-7) and whether rACE2 can prevent hypertension caused by Ang II infusion as a result of systemic versus local mechanisms of ACE2 activity amplification. rACE2 was infused via osmotic minipumps for 3 days in conscious mice or acutely in anesthetized mice. rACE2 caused a dose-dependent increase in serum ACE2 activity but had no effect on kidney or cardiac ACE2 activity. After Ang II infusion (40 pmol/min), rACE2 (1 mg/kg per day) resulted in normalization of systolic blood pressure and plasma Ang II. In acute studies, rACE2 (1 mg/kg) prevented the rapid hypertensive effect of Ang II (0.2 mg/kg), and this was associated with both a decrease in Ang II and an increase in Ang-(1-7) in plasma. Moreover, during infusion of Ang II, the effect of rACE2 on blood pressure was unaffected by a specific Ang-(1-7) receptor blocker, A779 (0.2 mg/kg), and infusing supraphysiologic levels of Ang-(1-7) (0.2 mg/kg) had no effect on blood pressure. We conclude that, during Ang II infusion, rACE2 effectively degrades Ang II and, in the process, normalizes blood pressure. The mechanism of rACE2 action results from an increase in systemic, not tissue, ACE2 activity and the lowering of plasma Ang II rather than the attendant increase in Ang-(1-7). Increasing ACE2 activity may provide a new therapeutic target in states of Ang II overactivity by enhancing its degradation, an approach that differs from the current focus on blocking Ang II formation and action.
- Published
- 2010
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41. Acute Renal Failure and Severe Hypertension from a Page Kidney Post-Transplant Biopsy
- Author
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Maria Aurora Posadas, Daniel Batlle, Vincent L. Yang, Muhammad Omer, and Bing Ho
- Subjects
Male ,Nephrology ,medicine.medical_specialty ,renin-angiotensin-aldosterone ,Urology ,lcsh:Medicine ,Blood Pressure ,lcsh:Technology ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Lymphocele ,Hematoma ,Perinephric Hematoma ,Internal medicine ,medicine ,Humans ,lcsh:Science ,Intensive care medicine ,General Environmental Science ,Creatinine ,Kidney ,Case Study ,lcsh:T ,Page kidney ,business.industry ,lcsh:R ,General Medicine ,Acute Kidney Injury ,Middle Aged ,medicine.disease ,Kidney Transplantation ,Treatment Outcome ,Blood pressure ,medicine.anatomical_structure ,chemistry ,Hypertension ,lcsh:Q ,transplant biopsy ,business - Abstract
Page kidney refers to a clinical picture characterized by acute onset of hypertension due to external compression of the kidneys from hematoma, tumor, lymphocele, or urinoma. Hypertension is believed to result from renin-angiotensin-aldosterone activation triggered by renal hypoperfusion and microvascular ischemia. Renal failure, in addition to hypertension, may occur in the setting of a single functional kidney or a diseased contralateral kidney. We report a case of a patient who had a transplant kidney biopsy complicated by a subcapsular perinephric hematoma. The patient presented with an acute increase in blood pressure and a rapid rise in serum creatinine following a transplant kidney routine biopsy. He underwent emergent evacuation of the perinephric hematoma, with consequent decrease of his blood pressure and return of serum creatinine back to his baseline level. Early recognition and rapid intervention are needed in order to correct hypertension and reverse acute renal failure in Page kidney occurring in renal transplant recipients.
- Published
- 2010
42. Effect of calcium-sensing receptor activation in models of autosomal recessive or dominant polycystic kidney disease
- Author
-
Xiaofang Wang, Daniel Batlle, Stefan Somlo, Vicente E. Torres, and Peter C. Harris
- Subjects
Male ,Receptors, Vasopressin ,medicine.medical_specialty ,Protein Serine-Threonine Kinases ,Kidney ,urologic and male genital diseases ,Rats, Mutant Strains ,Mice ,Internal medicine ,Arginine vasopressin receptor 2 ,Pkd2−/WS25 mouse ,Phenethylamines ,Cyclic AMP ,medicine ,Polycystic kidney disease ,Animals ,Humans ,RNA, Messenger ,PCK rat ,Polycystic Kidney, Autosomal Recessive ,Mice, Knockout ,Transplantation ,polycystic kidney disease ,Aniline Compounds ,Aquaporin 2 ,Propylamines ,business.industry ,Polycystic liver disease ,Pyruvate Dehydrogenase Acetyl-Transferring Kinase ,Kidney metabolism ,Polycystic Kidney, Autosomal Dominant ,medicine.disease ,Mice, Mutant Strains ,Autosomal Recessive Polycystic Kidney Disease ,Rats ,calcimimetic ,Disease Models, Animal ,Endocrinology ,medicine.anatomical_structure ,Nephrology ,Original Article ,Calcium ,Female ,Calcium-sensing receptor ,business ,Receptors, Calcium-Sensing - Abstract
Background. Antagonists of relevant Gs protein-coupled and agonists of relevant Gi protein-coupled receptors lower renal cAMP and inhibit growth of renal cysts in animal models of human ARPKD (PCK rat) and/or ADPKD (Pkd2−/WS25 mouse). A calcium-sensing receptor (CaR) is expressed in various tubular segments and couples to Gq, thereby activating phospholipase Cγ, InsP3 generation and calcium mobilization from intracellular stores, and Gi proteins. By both mechanisms, CaR activation could lower intracellular cAMP and inhibit renal cyst growth. Methods. PCK rat and Pkd2−/WS25 mouse littermates were fed rodent chow without or with R-568, a type 2 calcimimetic, at a concentration of 0.05% or 0.1% between 3 and 10 or 16 weeks of age. Histomorphometric analysis was performed with Meta-Morph software. Western analysis and immunohistochemical staining were performed using antibodies for aquaporin-2, urea transporter UT-A1 and CaR. Northern blot hybridization was used to quantify the expression of vasopressin V2 receptor and aquaporin 2 mRNAs. Cyclic AMP was measured using an enzyme immunoassay kit. Results. R-568 had no effect on kidney weight, cyst volume, plasma BUN concentration or severity of the polycystic liver disease. A significant reduction in renal interstitial fibrosis was detected in PCK rats, but not in Pkd2−/WS25 mice. R-568 administration, as anticipated, resulted in hypocalcemia and hyperphosphatemia, and significant increases in urine output, osmolar clearance, and urinary excretions of sodium, potassium and calcium. Conclusions. CaR activation had no detectable effect on cystogenesis in models of autosomal recessive or dominant polycystic kidney disease. The lack of protective effect could be due to the absence of CaR in the outer medullary and cortical collecting ducts, the reduction in extracellular calcium and the unaffected levels of renal cAMP and renal expression of cAMP-dependent genes. A possible beneficial effect on interstitial fibrosis deserves further study at more advanced stages of the disease.
- Published
- 2008
43. Angiotensin-converting enzyme 2 and the kidney
- Author
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María José Soler, Jan Wysocki, and Daniel Batlle
- Subjects
medicine.medical_specialty ,Angiotensin receptor ,Kidney ,biology ,urogenital system ,Chemistry ,Angiotensin-converting enzyme ,General Medicine ,Glomerulus (kidney) ,Angiotensin II ,Endocrinology ,medicine.anatomical_structure ,Internal medicine ,Angiotensin-converting enzyme 2 ,Renin–angiotensin system ,medicine ,Albuminuria ,biology.protein ,medicine.symptom ,hormones, hormone substitutes, and hormone antagonists - Abstract
Angiotensin-converting enzyme (ACE) 2 is a homologue of ACE with enzymatic activity that seems to counterbalance the angiotensin II-promoting effect of ACE. While ACE promotes angiotensin (Ang) II formation from Ang I, ACE2 degrades Ang II and Ang I. In this review, we discuss recent studies that have delineated the localization of ACE2 within the kidney, an organ that highly expresses this enzyme. In models of diabetic kidney, pharmacological ACE2 inhibition is associated with albuminuria and worsening of glomerular injury. Similarly, genetic ablation of ACE2 causes glomerular lesions in male mice and worsens the renal lesions seen in diabetic Akita mice. Taken together, these findings suggest that a decrease in ACE2 may be involved in diabetic kidney disease, possibly by disrupting the metabolism of angiotensin peptides in such a way that angiotensin II degradation within the glomerulus may be diminished.
- Published
- 2008
44. Angiotensin-converting enzyme 2: Possible role in hypertension and kidney disease
- Author
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Jan Wysocki, Daniel Batlle, and Francisco R. González-Pacheco
- Subjects
Nephrology ,medicine.medical_specialty ,Ace2 Knockout Mouse ,Angiotensin-Converting Enzyme Inhibitors ,Peptidyl-Dipeptidase A ,Pharmacology ,Kidney ,Article ,Renin-Angiotensin System ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,Humans ,Medicine ,Ace activity ,Catalytic efficiency ,chemistry.chemical_classification ,biology ,business.industry ,Angiotensin II ,Ace2 Gene ,Angiotensin-converting enzyme ,medicine.disease ,Enzyme ,Endocrinology ,Ace2 Gene Polymorphism ,chemistry ,Glomerular Injury ,Hypertension ,Angiotensin-converting enzyme 2 ,biology.protein ,Biological Assay ,Kidney Diseases ,Angiotensin-Converting Enzyme 2 ,Angiotensin I ,business ,hormones, hormone substitutes, and hormone antagonists ,Kidney disease - Abstract
The discovery of angiotensin-converting enzyme (ACE) 2 adds a new level of complexity to the understanding of the renin-angiotensin system. The high catalytic efficiency of ACE2 for the generation of angiotensin (ANG)-1-7 from ANG II suggests an important role of ACE2 in preventing ANG II accumulation, while at the same time enhancing ANG-1-7 formation. ACE and ACE2 may have counterbalancing functions and a regulatory role in fine-tuning the rate at which ANG peptides are formed and degraded. By counterregulating the actions of ACE on ANG II formation, ACE2 may play a role in maintaining a balanced status of the renninangiotensin system. This review focuses on the function of ACE2 and its possible roles in kidney disease and hypertension. Studies using models of ACE2 ablation and the pharmacologic administration of an ACE2 inhibitor suggest that decreased ACE2 activity alone or in combination with increased ACE activity may play a role in both diseases.
- Published
- 2008
45. ACE2 inhibition worsens glomerular injury in association with increased ACE expression in streptozotocin-induced diabetic mice
- Author
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Minghao Ye, Yashpal S. Kanwar, Jan Wysocki, J. Lloveras, Daniel Batlle, and María José Soler
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medicine.medical_specialty ,Kidney Glomerulus ,ACE2 ,Angiotensin-Converting Enzyme Inhibitors ,Peptidyl-Dipeptidase A ,albuminuria ,Streptozocin ,Diabetes Mellitus, Experimental ,Diabetic nephropathy ,Mice ,chemistry.chemical_compound ,Leucine ,Internal medicine ,Diabetes mellitus ,medicine ,Animals ,Diabetic Nephropathies ,ACE2 inhibition ,ACE ,Creatinine ,biology ,business.industry ,diabetic nephropathy ,Angiotensin II ,Imidazoles ,Angiotensin-converting enzyme ,Glomerulonephritis ,medicine.disease ,Streptozotocin ,Up-Regulation ,Endocrinology ,chemistry ,Nephrology ,Angiotensin-converting enzyme 2 ,biology.protein ,Angiotensin-Converting Enzyme 2 ,business ,renal histology ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Angiotensin converting enzyme 2 (ACE2) is localized to the glomerular epithelial cells. Since ACE2 promotes the degradation of angiotensin II, a decrease in ACE2 activity could lead to the development of glomerular injury. We gave a specific ACE2 inhibitor, MLN-4760, for 4 weeks to mice rendered diabetic with streptozotocin. The urinary albumin/creatinine ratio was increased along with expansion of the glomerular matrix in diabetic mice treated with the inhibitor compared to the vehicle-treated mice. Glomerular staining of ACE was increased in the diabetic group and was further significantly increased in the diabetic group treated with MLN-4760. In renal vessels, ACE expression was also increased in the diabetic mice and, again, further increased in those diabetic mice treated with the ACE2 inhibitor. Our study shows that chronic pharmacologic ACE2 inhibition worsens glomerular injury in streptozotocin-induced diabetic mice in association with increased ACE expression.
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- 2007
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46. Stabilization of Glomerular Filtration Rate in Advanced Chronic Kidney Disease: A Two-Year Follow-up of a Cohort of Chronic Kidney Disease Patients Stages 4 and 5
- Author
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James Paparello, Cybele Ghossein, Shubhada N. Ahya, Vijayachitra Madhan, William Schlueter, Neenoo Khosla, Jie Huang, Daniel Batlle, Laura Nishi, Andres Serrano, Anupama Gangavathi, and Paramesh Ramadugu
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Adult ,Male ,medicine.medical_specialty ,Anemia ,medicine.medical_treatment ,Renal function ,urologic and male genital diseases ,Cohort Studies ,Renal Dialysis ,Internal medicine ,medicine ,Humans ,Intensive care medicine ,Dialysis ,Aged ,Aged, 80 and over ,urogenital system ,business.industry ,Mortality rate ,Middle Aged ,medicine.disease ,female genital diseases and pregnancy complications ,Transplantation ,Treatment Outcome ,Nephrology ,Chronic Disease ,Cohort ,Kidney Failure, Chronic ,Female ,Kidney Diseases ,business ,Glomerular Filtration Rate ,Cohort study ,Kidney disease - Abstract
This study examines whether stabilization of the glomerular filtration rate (GFR) is possible in patients with advanced chronic kidney disease (CKD), managed in a CKD clinic. A cohort of 82 patients with stages 4 and 5 CKD was followed for a period of 2 years after initiation of erythropoietin for anemia to determine the GFR and the frequency of primary outcomes (dialysis, transplantation, or death). GFR, calculated by the abbreviated Modification of Diet in Renal Disease formula, was determined every 3 months. After 24 months, 35 subjects (43%) developed a primary outcome. Controlled for other risk factors, the risk of having a primary outcome increased 19.7% for every unit that the GFR decreased (95% confidence interval [CI], 11.9%-26.8%, P.001) and decreased 21.7% for every unit that the hemoglobin increased (95% CI, 0.5%-38.4%, P.001). Blacks had a 3.1 times higher risk (95% CI, 1.4-6.9, P = .006) of developing a primary outcome than other ethnicities. In subjects who did not develop primary outcomes (n = 47 or 57%), GFR remained unchanged (19.5 +/- 9.1 at the end of the study v 20.8 +/- 5.3 mL/min/1.73 m(2) at baseline, P = .16). The standardized mortality rate was 4.75 and 9.77 per 100 person-year for stages 4 and 5, respectively. We conclude that stabilization of GFR over a 2-year period can be achieved in many patients with advanced CKD treated with erythropoietin in a CKD clinic. Although the precise reason for the stabilization of GFR cannot be elucidated from this study, our data are "proof of concept" that CKD outcomes can be improved in a CKD clinic setting.
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- 2007
47. Intestinal Ileus as a Possible Cause of Hypobicarbonatemia
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Yemin Yuan, Rajani K. Chilakapati, Giuseppe Rombolà, Alexander J. Ghanayem, Daniel Batlle, Andres Serrano, Cathy Stephen, and Jeffery Alberts
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Male ,metabolic acidosis ,medicine.medical_specialty ,Ileus ,Bicarbonate ,lcsh:Medicine ,Anion gap ,hypobicarbonatemia ,intestinal bicarbonate secretion ,lcsh:Technology ,General Biochemistry, Genetics and Molecular Biology ,Diagnosis, Differential ,intestinal ileus ,Excretion ,chemistry.chemical_compound ,Internal medicine ,hypokalemia ,medicine ,Humans ,lcsh:Science ,General Environmental Science ,Case Study ,lcsh:T ,Pancreatitis, Acute Necrotizing ,business.industry ,lcsh:R ,Intestinal Pseudo-Obstruction ,Transverse colon ,Alkalosis ,Metabolic acidosis ,General Medicine ,Middle Aged ,medicine.disease ,digestive system diseases ,Endocrinology ,chemistry ,Respiratory alkalosis ,Pancreatitis ,lcsh:Q ,business - Abstract
The possible occurrence of metabolic acidosis in patients with intestinal ileus is not well recognized. We describe a patient with acute alcohol-induced pancreatitis and a large transverse colon ileus in which plasma bicarbonate dropped rapidly in the absence of an increase in the plasma anion gap. The urinary anion gap and ammonium excretion were consistent with an appropriate renal response to metabolic acidosis and against the possibility of respiratory alkalosis. The cause of the falling plasma bicarbonate was ascribed to intestinal bicarbonate sequestration owing to the enhancement of chloride-bicarbonate exchange in a dilated paralyzed colon.
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- 2007
48. Abstract P637: Pigment Epithelium Derived Factor (PEDF) Deficiency Increases Blood Pressure And Accentuates Glomerular Pathology In Diabetic Mice
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Olga V. Volpert, Jan Wysocki, Daniel Batlle, and Minghao Ye
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medicine.medical_specialty ,Kidney ,Pathology ,business.industry ,Arbitrary unit ,Wild type ,medicine.disease ,Podocyte ,PEDF ,Endocrinology ,medicine.anatomical_structure ,Blood pressure ,Downregulation and upregulation ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,business - Abstract
Pigment Epithelium Derived Factor (PEDF) encoded by SERPINF1 gene has potent anti-angiogenic and cytoprotective activities. It has been reported that PEDF protein levels are reduced in the kidneys of rodents with experimentally induced diabetes. However the effect of PEDF on blood pressure has not been elucidated. Here, we used SERPINF1 KO mice to examine the impact of PEDF deficiency on kidney pathology and blood pressure in the STZ-induced mouse model of diabetes. Twelve weeks after diabetes induction by STZ, SERPINF1 KO mice showed exacerbated glomerular damage with ~ 2-fold increase in mesangial matrix (2.8±0.14 vs. 1.2±0.14 arbitrary units, p Our data indicate that global PEDF deficiency intensifies glomerular injury in STZ-induced mouse model of diabetes. An important drawback of most murine models of diabetic kidney disease is the lack of hypertension, a known key factor that accelerates progression to CKD in humans. In contrast, we found that STZ-treated SERPINF1-/- mice become hypertensive. Together, our findings point to SERPINF1-/- mice as an attractive model to study diabetic kidney disease and hypertension in mice and suggest an important causative role of PEDF downregulation in glomerular pathology in diabetes.
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- 2015
49. ANGIOTENSINS AND THE HEART: IS ANG 1-7 CARDIOPROTECTIVE?
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Jan Wysocki, Daniel Batlle, and Lisa D. Wilsbacher
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Male ,medicine.medical_specialty ,Diastole ,Vasodilation ,Blood Pressure ,Left ventricular hypertrophy ,Article ,Rats, Sprague-Dawley ,Desoxycorticosterone Acetate ,Afterload ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,Medicine ,Animals ,Calcium Signaling ,Heart Failure, Diastolic ,Dose-Response Relationship, Drug ,business.industry ,medicine.disease ,Hydralazine ,Angiotensin II ,Peptide Fragments ,Rats ,Disease Models, Animal ,Blood pressure ,Endocrinology ,Heart failure ,Hypertension ,cardiovascular system ,Calcium ,Angiotensin I ,Rats, Transgenic ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
See related article, pp 389–395 Left ventricular hypertrophy is the most common cardiac complication of hypertension. Although the initial adaptations associated with cardiac hypertrophy are compensatory, ultimately abnormal ventricular function including diastolic dysfunction (impaired relaxation) and often heart failure may develop.1 Activation of the renin–angiotensin system and its main effector peptide angiotensin II (Ang II), acting on the Ang II type 1 receptor, has been considered an important part of the cascade leading to left ventricular hypertrophy and cardiac fibrosis.1,2 Recent work, however, examining the effects of Ang II infusion using cardiomyocyte and vascular smooth muscle–specific Ang II type 1 receptor knockouts suggests that the hypertension-induced increase in afterload, rather than direct Ang II–Ang II type 1 receptor signaling in the heart, is the key factor that promotes hypertrophic responses.2 The renin–angiotensin system peptide Ang-(1–7), which is generated from Ang II by the action of carboxypeptidases, such as ACE2,3 exhibits actions that are mainly opposite to those of Ang II, including vasodilatory and antifibrotic effects.4 In this issue, Machado de Almeida et al5 report a series of interesting observations that suggest that in an Ang-(1–7) transgenic line, TGR(A1–7)3292, there is cardioprotection from deoxycorticosterone acetate (DOCA)–salt induced hypertension which is independent of blood pressure. The latter conclusion is not unexpected considering the strong evidence against an antihypertensive effect of Ang-(1–7): (1) acute infusions of supraphysiologic concentrations of this peptide do not lower blood pressure in mice,3 (2) a 4-week continuous infusion of Ang-(1–7) did not decrease blood pressure in DOCA-treated Sprague-Dawley (SD) rats,6 (3) acutely Ang-(1–7) does not attenuate the hypertensive effect of infused Ang II, and a blocker …
- Published
- 2015
50. Cross-Disciplinary Biomarkers Research: Lessons Learned by the CKD Biomarkers Consortium
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Chi-yuan Hsu, John W. Kusek, Casey M. Rebholz, Robert G. Nelson, Sushrut S. Waikar, Ramachandran S. Vasan, Jon B. Klein, Joseph V. Bonventre, Harold I. Feldman, Jennifer E. Van Eyk, Michael Mauer, Paul L. Kimmel, Brad H. Rovin, Daniel Batlle, Josef Coresh, Krista M. Whitehead, Mark E. Molitch, Venkata S. Sabbisetti, Erwin P. Bottinger, Lesley A. Inker, Shawn Ballard, Theodore E. Mifflin, Kathleen D. Liu, Gary L. Nelsestuen, and John H. Eckfeldt
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Quality Control ,Transplantation ,medicine.medical_specialty ,Pathology ,Biomedical Research ,Epidemiology ,business.industry ,Cross disciplinary ,Interdisciplinary Studies ,Critical Care and Intensive Care Medicine ,Special Features ,Biospecimen Collection ,Specimen Handling ,Nephrology ,External quality assessment ,Medicine ,Biomarker (medicine) ,Humans ,Medical physics ,Biomarker discovery ,Renal Insufficiency, Chronic ,business ,Biomarkers - Abstract
Significant advances are needed to improve the diagnosis, prognosis, and management of persons with CKD. Discovery of new biomarkers and improvements in currently available biomarkers for CKD hold great promise to achieve these necessary advances. Interest in identification and evaluation of biomarkers for CKD has increased substantially over the past decade. In 2009, the National Institute of Diabetes and Digestive and Kidney Diseases established the CKD Biomarkers Consortium (http://www.ckdbiomarkersconsortium.org/), a multidisciplinary, collaborative study group located at over a dozen academic medical centers. The main objective of the consortium was to evaluate new biomarkers for purposes related to CKD in established prospective cohorts, including those enriched for CKD. During the first 5 years of the consortium, many insights into collaborative biomarker research were gained that may be useful to other investigators involved in biomarkers research. These lessons learned are outlined in this Special Feature and include a wide range of issues related to biospecimen collection, storage, and retrieval, and the internal and external quality assessment of laboratories that performed the assays. The authors propose that investigations involving biomarker discovery and validation are greatly enhanced by establishing and following explicit quality control metrics, including the use of blind replicate and proficiency samples, by carefully considering the conditions under which specimens are collected, handled, and stored, and by conducting pilot and feasibility studies when there are concerns about the condition of the specimens or the accuracy or reproducibility of the assays.
- Published
- 2015
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