10 results on '"M. Cortiana"'
Search Results
2. Effects of simulated altitude (normobaric hypoxia) on cardiorespiratory parameters and circulating endothelial precursors in healthy subjects
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Maria Alfonsina Desiderio, Elisa Ridolfi, Alberto Pierini, I. Silvestris, Fabrizio Giofrè, Emanuela Matteucci, Roberta Paliotti, Michele M. Ciulla, Maddalena Zanardelli, Agostino Cortelezzi, Fabio Magrini, and M. Cortiana more...
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Pulmonary and Respiratory Medicine ,Male ,medicine.medical_specialty ,Respiratory physiology ,Biology ,chemistry.chemical_compound ,Heart Rate ,Internal medicine ,Heart rate ,medicine ,Humans ,Hypoxia ,lcsh:RC705-779 ,Hepatocyte Growth Factor ,Research ,Altitude ,Endothelial Cells ,Cardiorespiratory fitness ,lcsh:Diseases of the respiratory system ,Hypoxia (medical) ,Flow Cytometry ,Vascular endothelial growth factor ,Blood pressure ,Endocrinology ,chemistry ,Erythropoietin ,Immunology ,Respiratory Mechanics ,cardiovascular system ,Hepatocyte growth factor ,medicine.symptom ,circulatory and respiratory physiology ,medicine.drug - Abstract
Background Circulating Endothelial Precursors (PB-EPCs) are involved in the maintenance of the endothelial compartment being promptly mobilized after injuries of the vascular endothelium, but the effects of a brief normobaric hypoxia on PB-EPCs in healthy subjects are scarcely studied. Methods Clinical and molecular parameters were investigated in healthy subjects (n = 8) in basal conditions (T0) and after 1 h of normobaric hypoxia (T1), with Inspiratory Fraction of Oxygen set at 11.2% simulating 4850 mt of altitude. Blood samples were obtained at T0 and T1, as well as 7 days after hypoxia (T2). Results In all studied subjects we observed a prompt and significant increase in PB-EPCs, with a return to basal value at T2. The induction of hypoxia was confirmed by Alveolar Oxygen Partial Pressure (PAO2) and Spot Oxygen Saturation decreases. Heart rate increased, but arterial pressure and respiratory response were unaffected. The change in PB-EPCs percent from T0 to T1 was inversely related to PAO2 at T1. Rapid (T1) increases in serum levels of hepatocyte growth factor and erythropoietin, as well as in cellular PB-EPCs-expression of Hypoxia Inducible Factor-1α were observed. Conclusion In conclusion, the endothelial compartment seems quite responsive to standardized brief hypoxia, possibly important for PB-EPCs activation and recruitment. more...
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- 2007
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3. Circulating endothelial progenitor cell colony-forming capacity in healthy subjects: how does an endothelial colony look like?
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Lorenza Lazzari, I. Silvestris, M. Cortiana, Michele M. Ciulla, Rosaria Giordano, Alessandra Giorgetti, and Roberta Paliotti
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medicine.medical_specialty ,business.industry ,Cèl·lules ,Cells ,Colony-Forming Units Assay ,Healthy subjects ,Cell movement ,Endothelial progenitor cell ,Endoteli ,Blood vessels ,Reference values ,Internal medicine ,Immunology ,Cardiology ,medicine ,Endothelium ,Stem cell ,Cardiology and Cardiovascular Medicine ,business ,Vasos sanguinis - Abstract
We read with great interest the recent report in the American Journal of Cardiology by Hoetzer et al.1 The investigators addressed the question of whether in healthy, middle-aged subjects circulating endothelial progenitor cell (EPC) number and function (capacity to form colonies) are different in women and men. It was a study well conducted by an experienced team. more...
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- 2007
4. Bone marrow endothelial progenitors are defective in systemic sclerosis
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Cinzia Scavullo, M. Cortiana, Claudio Vitali, Chiara Borsotti, Paolo Fraticelli, Agostino Cortelezzi, Davide Soligo, N. Quirici, Giorgio Lambertenghi-Deliliers, D.P. Comina, Armando Gabrielli, Nicoletta Del Papa, and Wanda Maglione more...
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Stromal cell ,Cellular differentiation ,Immunology ,Bone Marrow Cells ,Microcirculation ,Vasculogenesis ,Rheumatology ,Antigens, CD ,medicine ,Immunology and Allergy ,Humans ,Pharmacology (medical) ,AC133 Antigen ,Progenitor cell ,Cells, Cultured ,Aged ,Glycoproteins ,Scleroderma, Systemic ,Neovascularization, Pathologic ,business.industry ,Mesenchymal stem cell ,Endothelial Cells ,Cell Differentiation ,Mesenchymal Stem Cells ,Middle Aged ,Flow Cytometry ,Haematopoiesis ,medicine.anatomical_structure ,cardiovascular system ,Leukocyte Common Antigens ,Female ,Bone marrow ,business ,Peptides - Abstract
Objective Vascular abnormalities represent the main component of the pathobiology of systemic sclerosis (SSc), progressing from structural derangements of the microcirculation with abortive neoangiogenesis to final vessel loss. Since circulating endothelial progenitor cells (EPCs) are important in the vascular repair process, we undertook this study to examine their numbers in the peripheral blood (PB) of SSc patients and to evaluate whether their status is related to impaired quantitative and/or qualitative aspects of the bone marrow (BM) microenvironment. Methods Circulating EPCs from 62 SSc patients were evaluated by flow cytometry and characterized as CD45 negative and CD133 positive. BM EPCs, identified as CD133 positive, were isolated from 14 SSc patients and grown to induce endothelial differentiation. In addition, progenitor numbers and functional properties of hematopoietic and stromal compartments were analyzed by various assays. Results We found that EPCs were detectable in the PB of patients with SSc, and their number was significantly increased in patients with early-stage disease but not in those with late-stage disease. All of the examined BM samples contained reduced numbers of EPCs and stromal cells, both of which were functionally impaired. Both endothelial and stromal progenitors expressed vascular endothelial growth factor receptor, indicating that BM is strongly induced to differentiate into the endothelial lineage; furthermore, only BM EPCs from patients with early disease led to endothelial differentiation in vitro. Conclusion This study provides the first demonstration that in SSc, there is a complex impairment in the BM microenvironment involving both the endothelial and mesenchymal stem cell compartments and that this impairment might play a role in defective vasculogenesis in scleroderma. more...
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- 2006
5. Endothelial colony forming capacity is related to C-reactive protein levels in healthy subjects
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Federico De Marco, Michele M. Ciulla, Roberta Paliotti, Alessandra Giorgetti, I. Silvestris, G. Annoni, Paolo Rebulla, Agostino Cortelezzi, Lorenza Lazzari, M. Cortiana, Elisa Montelatici, Anna Valeria Fiore, Fabio Magrini, and Rosaria Giordano more...
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Adult ,Male ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Settore MED/09 - Medicina Interna ,Angiogenesis ,Neovascularization, Physiologic ,Biology ,Flow cytometry ,Cohort Studies ,Colony-Forming Units Assay ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Developmental Neuroscience ,Reference Values ,Risk Factors ,Internal medicine ,medicine ,Humans ,Progenitor cell ,Clonogenic assay ,Sex Characteristics ,Tissue Inhibitor of Metalloproteinase-1 ,medicine.diagnostic_test ,C-reactive protein ,Endothelial Cells ,Middle Aged ,Flow Cytometry ,Hematopoietic Stem Cells ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,Endocrinology ,C-Reactive Protein ,Neurology ,chemistry ,Cardiovascular Diseases ,Mitogen-activated protein kinase ,Immunology ,biology.protein ,Female ,Endothelial progenitor cells ,High-sensitivity c-reactive protein ,Reparative medicine ,Settore MED/15 - Malattie del Sangue - Abstract
The majority of clinical studies on endothelial progenitor cells (EPCs) focuses on the role of these cells in cardiovascular diseases and no systematic studies exist regarding their variations in healthy subjects. In order to define the burden of angiogenesis in physiological conditions we assessed the frequency of peripheral blood endothelial colonies (PB-ECs) and their relation with other factors possibly involved in their function such as high-sensitivity C-reactive protein (hs-CRP), endothelial cell-specific mitogen factor (VEGF) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in a highly selected healthy population. A PB sample was obtained from 37/47 healthy subjects (age 40.2+/-15.0yrs; M/F 15/22) without known cardiovascular risk factors. The serum level of hs-CRP, VEGF, TIMP-1, the frequency of PB-ECs by clonogenic assay, and the number of early EPCs and late EPCs by flow cytometry analysis were evaluated. PB-ECs were formed by 40.5% of studied subjects with a mean of 0.40+/-0.82 colonies/10(6) cells. The differences in the frequency of colony formation between genders were not statistically significant. The subjects with PB-ECs were characterized by higher values of hs-CRP, when compared with those not forming colonies, 0.276+/-0.230 vs 0.095+/-0.077 mg/l (p=0.003) respectively, and of VEGF, 328.3+/-162.9 vs 202.68+/-118.53 pg/ml (p=0.02). No significant differences were found in TIMP-1 values. The EPC clonogenic potential seems to be related to hs-CRP and VEGF levels even in healthy population supporting the concept that these mediators are involved in physiological ECs function. more...
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- 2006
6. Endothelial precursors and mature endothelial cells are increased in the peripheral blood of myelodysplastic syndromes
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Francesco Bertolini, Agostino Cortelezzi, L. Moronetti Mazzeo, M.C. Pasquini, Nicola Stefano Fracchiolla, Umberto Gianelli, Patrizia Mancuso, G. Lambertenghi Deliliers, M. Cortiana, M. Pomati, Franco Somalvico, Giancarlo Pruneri, and I. Silvestris more...
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Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Myeloid ,Angiogenesis ,Basic fibroblast growth factor ,Cell Count ,chemistry.chemical_compound ,Bone Marrow ,hemic and lymphatic diseases ,medicine ,Humans ,Aged ,Neovascularization, Pathologic ,business.industry ,Myelodysplastic syndromes ,Stem Cells ,Myeloid leukemia ,Endothelial Cells ,Hematology ,Middle Aged ,medicine.disease ,Flow Cytometry ,Vascular endothelial growth factor ,medicine.anatomical_structure ,Oncology ,chemistry ,International Prognostic Scoring System ,Myelodysplastic Syndromes ,cardiovascular system ,Cancer research ,Female ,Bone marrow ,business ,Biomarkers - Abstract
Increased angiogenesis has been demonstrated to be a significant prognostic factor in many solid tumors. In the oncohematological setting, it has been associated with myelodysplastic syndromes (MDS), chronic myeloid leukemia, acute lymphoid, and myeloid leukemias. Recently, increased circulating endothelial cells (CECs) have been associated with breast cancer and non-Hodgkin lymphoma (NHL). Based on these premises we analysed total and activated CECs, and endothelial precursors (CEPs) in 50 MDS patients and 20 healthy donors. CECs and CEPs were quantified by flow cytometry. CEC levels were compared with bone marrow (BM) microvessel density (MVD). In addition, some angiogenic factors, namely vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and soluble VEGF-Receptor2 (VEGFR2), were tested in the sera from 25 MDS patients. Total, activated CECs and CEPs were significantly increased in MDS when compared to control group (p < 0.0001); whereas in the MDS cases no association was found with French-American-British (FAB), International Prognostic Scoring System (IPSS) subtypes or survival. Patients with higher CECs also showed higher MVD. Among the cytokines analysed, sVEGFR2 was significantly higher in the lower IPSS risk classes, while the levels of bFGF directly correlated with total and activated CECs. Taken together these data strengthen the hypothesis of a possible role of angiogenesis in MDS pathogenesis. more...
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- 2005
7. High-altitude trekking in the Himalayas increases the activity of circulating endothelial cells
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G. Annoni, M. Cortiana, Roberta Paliotti, C. De Asmundis, Michele M. Ciulla, Alessandra Giorgetti, Paolo Rebulla, Lorenza Lazzari, A. V. Fiore, Elisa Montelatici, Fabio Magrini, I. Silvestris, Agostino Cortelezzi, Hematology, Internal Medicine Specializations, and Cardio-vascular diseases more...
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Adult ,Male ,medicine.medical_specialty ,Vascular homeostasis ,India ,Biology ,Altitude Sickness ,Oxygen Consumption ,Internal medicine ,medicine ,Humans ,Progenitor cell ,Clonogenic assay ,Travel ,exercise ,Altitude ,Healthy subjects ,Hematology ,Effects of high altitude on humans ,Hypoxia (medical) ,hypobaric hypoxia ,Endothelial stem cell ,Endocrinology ,Italy ,endothelial cell ,cardiovascular system ,Hypobaric hypoxia ,Endothelium, Vascular ,medicine.symptom - Abstract
Circulating endothelial progenitor cells (EPCs) are believed to contribute to vascular homeostasis; unfortunately, the response of EPCs in physiological conditions remains largely unknown. Herein we report our observations of a 44-year-old healthy subject after a trek in the Himalayas that support high-altitude hypoxia and exercise oxygen demands are strong stimuli for clonogenic endothelial cell activation and activity, as shown by the increase in the number of mature EPCs and in the endothelial colony-forming unit capacity. Both of these effects were completely reverted at sea level, 45 days after the subject's trek. Am. J. Hematol. 79:76-78, 2005. 2005 Wiley-Liss, In more...
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- 2005
8. Pulmonary Arterial Hypertension in Idiopathic Myelofibrosis Is Associated with an Alterated Angiogenic Status
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I. Silvestris, Giorgio Lambertenghi Deliliers, Paola Bianchi, Agostino Cortelezzi, Nicoletta Del Papa, Fabiola B. Sozzi, Giuseppe Gritti, Rossella Calori, M.C. Pasquini, and M. Cortiana
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CD31 ,Pathology ,medicine.medical_specialty ,Lung ,Endothelium ,medicine.diagnostic_test ,business.industry ,Immunology ,Cell Biology ,Hematology ,Doppler echocardiography ,medicine.disease ,Biochemistry ,Endothelin 1 ,Pulmonary hypertension ,Gastroenterology ,Pulmonary function testing ,Pathogenesis ,medicine.anatomical_structure ,Internal medicine ,medicine ,business - Abstract
Aim: Idiopathic myelofibrosis (IMF) is associated with an increased risk of pulmonary arterial hypertension (PAH). We investigated the prevalence of PAH in a large cohort of IMF patients and its possible association with some mechanisms known to be involved in PAH generation. Materials and methods: Systolic pulmonary arterial pressure (sPAP) was evaluated by transthoracic doppler echocardiography in 37 IMF patients (20 males and 17 females; median age 65.1 years, range 37–83). The patients with sPAP ≥35 mmHg were further evaluated by high-resolution lung CT, lung perfusion scintigraphy, arterial blood gas analysis and pulmonary function tests (PFT), including lung carbon monoxide diffusing capacity. The patients’ circulating endothelial cells (CECs: CD45−, CD146+, CD31+) and endothelial progenitor cells (EPCs: CD45−, CD133+, CD34+) were evaluated using 4-parameter 3-colour flow cytometry and compared with those of 11 age-matched healthy controls. Serum VEGF, ET-1, TGFβ, PDGF-AB and sE-selectin levels were also determined. Results: PAH (sPAP ≥35 mmHg) was documented in 13 patients (35%), six of whom (16% of the whole series) had mild PAH (sPAP ≥45 mmHg); three of the patients with PAH were symptomatic. No case of pulmonary hypertension was due to cardiac or lung disease, or thromboembolic events. Median EPC levels were higher in the IMF patients than controls (0.78/μL [range 0–4.3] vs 0.32/μL [range 0.11–0.65]; p=0.003), and lower in the IMF patients with PAH than in those with sPAP Conclusion: This study shows that PAH is frequent in IMF patients without any known pulmonary or heart complications, being moderate in a subset in whom close follow-up is advisable. IMF and PAH have abnormal angiogenesis in common, and the patients with IMF complicated by PAH had markedly high VEGF levels together with relatively low EPC levels. Our findings suggest that PAH in this setting is associated with abnormalities in angiogenic status rather than the direct endothelial damage expressed by the increased CEC shedding found in other diseases associated with PAH. These results support the hypothesis that common mechanism(s) may be involved in the pathogenesis of IMF and PAH, thus warranting studies of CEP clonality. more...
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- 2006
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9. Pathogenetic Role of Mature and Progenitor Endothelial Cells in Pre-Eclampsia
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Giorgio Lambertenghi Deliliers, Agostino Cortelezzi, Nicola Stefano Fracchiolla, V. Cozzi, I. Silvestris, Giorgio Pardi, M.C. Pasquini, Irene Cetin, and M. Cortiana
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CD31 ,Pathology ,medicine.medical_specialty ,Eclampsia ,Endothelium ,business.industry ,Angiogenesis ,Immunology ,Placentation ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Sickle cell anemia ,Andrology ,medicine.anatomical_structure ,Vasculogenesis ,Coagulopathy ,Medicine ,business - Abstract
Pre-eclampsia (PE) is a pregnancy-associated disorder of unknown cause. The pathological changes associated with PE (edema, proteinuria, coagulopathy, and renal and hepatic abnormalities) suggest a systemic maternal vascular dysfunction. PE is also associated with placental development defects and fetal growth restriction. The generation of vessels is divided into angiogenesis (the sprouting of capillaries from pre-existing vessels) and vasculogenesis, the development of blood vessels from in situ differentiating endothelial cells (ECs), which has been considered as occurring only in the prenatal period. However, circulating endothelial precursors (CEPs) are also present in the peripheral blood (PB) of adults and, in the case of pregnant women, may play important roles in vascularising the uterine endometrium at the time of embryo implantation and placentation. Furthermore, it has been found that the number of mature circulating endothelial cells (CECs) is increased in the PB of patients affected by cancer, sickle cell anemia, myocardial infarction or infections, but they have not been extensively studied in PE patients. Inorder to test the hypothesis that CEPs may be involved in the pathogenesis of PE and that CECs may represent a marker of the severity of ongoing vascular damage, we used flow cytometry to quantify the number of CEPs and CECs in 17 PE patients and 13 healthy pregnant women of similar gestational age. Immunocytofluorimetric assays showed that resting CECs were negative for CD45 but positive for P1H12 and CD31, whereas CEPs were positive for CD133 and CD34. Our 13 healthy controls had a mean number of 20.8 CECs/ml (range 9.22–77.11) and 1.05 CEPs/ml (range 0.07–3.43); the corresponding numbers in 27 samples from the 17 PE cases were 25.46/ml (range 1.65–126.34) and 0.42/ml (range 0–15.45). The PE cases had significantly fewer CEPs than the controls (p more...
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- 2006
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10. Stem Cells Defects in Systemic Sclerosis
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Agostino Cortelezzi, Chiara Borsotti, Davide Soligo, D.P. Comina, N. Quirici, Cinzia Scavullo, Giorgio Lambertenghi Deliliers, M. Cortiana, Wanda Maglione, and Nicoletta Del Papa
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Pathology ,medicine.medical_specialty ,Stromal cell ,Angiogenesis ,Immunology ,Mesenchymal stem cell ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Molecular biology ,Haematopoiesis ,medicine.anatomical_structure ,Vasculogenesis ,medicine ,Bone marrow ,Stem cell ,Progenitor cell - Abstract
Systemic Sclerosis (SSc) is a connective tissue disease characterized by early generalized microangiopathy and culminating in systemic fibrosis. Recent studies have provided evidence that SSc is associated with a reactive but ineffective angiogenesis, so that the disease finally leads to the irreversible loss of capillaries. Aim of the study was to investigate whether impaired vasculogenesis in SSc is due to defective characteristics in BM microenvironment. Peripheral blood (PB) samples were collected from 70 patients (pts): circulating endothelial progenitors (CEPs) were characterized as CD45−/CD133+ and evaluated by flow cytometry. BM samples were collected from 14 SSc pts and hematopoiesis evaluated by various assays. CD133+ cells were isolated by immunomagnetic sorting (IMS) and grown in order to induce endothelial differentiation. Long-term bone marrow cultures (LTBMC) were assessed and the number of stromal clonogenic precursors evaluated by a CFU-F (colony-forming unit fibroblast) assay. Mesenchymal stem cells (MSC) were separated by IMS for the expression of the nerve growth factor-receptor (NGF-R+) and grown in order to assess the clonogenic potential and the proliferative capacity, while their multipotential differentiation ability was determined after culture in different conditioned media. Phenotypic analysis of BM mononuclear cells showed a greater expression of the surface markers P1H12 and CD105 TGF-β receptor (1.2%±0.6 vs 0.5%±0.1 in normal controls, p=0.01 and 9.9%±5 vs 4.7%±3, p=0.02 respectively), but lower percentages of NGF-R+ stromal cell precursors (0.73±0.5 vs 1.61±0.6, p=0.02) and CD133+ cells (0.36%±0.4 vs 1.2%±0.8, p=0.05). On the contrary, the absolute number of CEPs in PB was higher in patients with SSc than in healthy controls (mean values 2.1 cells/μL vs 0.26 cells/μL, p=0.04). When BM CD133+ cells were grown in the presence of VEGF, only 3/12 cases gave endothelial differentiation, but always with a reduced proliferative ability. All pts showed a defective stromal compartment and a reduced number of BM stromal precursors, as detected by the LTBMC and by the lower CFU-F frequency (4%±3.2 vs 43%±19.8/1x10(e)6 LDMNCs, p=0.002 and 7±12.8 vs 69±61/1x10(e)5 NGF-R+ cells, p=0.01). Interestingly, NGF-R+ MSC overexpressed KDR and CD117 (26.4%±7.4 vs 4.6%±1.7, p=0.01 and 87.7%±5.1 vs 57.6%±11, p=0.03 respectively): when grown in the presence of VEGF they gave rise to endothelial colonies, only in 2/8 cases they formed a confluent layer with fibroblastic morphology but a reduced proliferative ability, while in the presence of adipogenic or osteogenic inductive media they failed to origin specific differentiation. Moreover, all “in vitro” differentiated endothelial cells even before activation showed high levels of CD62-E, VCAM-1 and CD105 expression, suggestive of the presence of increased levels of proangiogenic factors in BM. The results of this study provide evidence that patients with SSc have a stem cell defect involving both the hematopoietic and the stromal cells compartments. The higher expression of KDR on NGF-R+ cells suggests a role for VEGF in inducing endothelial differentiation of MSC, so resulting in a depletion of stromal precursors. The continuous recruitment of endothelial progenitors to sites of vascular injury, suggested by the high numbers of CEPs in PB, might lead to the irreversible BM damage we observed. more...
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- 2005
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