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Objective: To determine the effect of Bryophyllum pinnatum treatment in modulating immune response and the pregnancy outcomes of pregnant pristane-induced lupus mice model. Methods: Sixteen Balb/c mice were intraperitoneally injected with single 0.5 cc pristane to induce lupus manifestations. After 12 weeks of injection, mice were mated and considered as gestational day 0 (GD0). Mice were divided into 4 groups based on the dosages of Bryophyllum pinnatum : control (no treatment), B1 (10.5 mg/kg), B2 (21 mg/kg), and B3 (42 mg/kg). The treatment was given orally every day started from GD9 until 9 days. At the end of the study, blood pressure and fetal size were measured. Serum anti-dsDNA and urine albumin levels were measured by ELISA. Spleen T helper (Th) and mature B cells percentages were measured by flow cytometry. Results: Administration of Bryophyllum pinnatum reduced the percentages of Th1 (p=0.006), Th2 (p=0.005), Th17 (p=0.000), and mature B cells (p=0.007) in dose-dependent manner. B1 and B2 had significantly lower of systolic blood pressure compared to control (p=0.026 and p=0.022 respectively). Significantly lower of anti-dsDNA levels were found in B1 group compared to control (p=0.014). However, no significantly different of urine albumin levels were found between groups. Bryophyllum pinnatum also significantly increased the fetus body weight in dose-dependent manner (p=0.000). Conclusion: Treatment of Bryophyllum pinnatum could improve the pregnancy outcome and modulate the immune response in pregnant pristane-induced lupus mice. Therefore, Bryophyllum pinnatum is a potential herb which can be developed as an immunosuppressive agent in the future.

Latar belakang: Perdarahan post partum dapat disebabkan oleh atonia uteri, robekan jalan lahir, retensio plasenta, inversio uteri dan pembekuan darah. Pada umumnya dengan melakukan senam nifas maka dapat mempercepat proses pemulihan kondisi ibu setelah melahirkan dan mencegah komplikasi yang mungkin terjadi selama masa nifas. Penelitian ini bertujuan mengetahui pengaruh senam nifas terhadap penurunan tinggi fundus uteri pada ibu post partum di Wilayah Kerja Puskesmas Temindung Samarinda. Metode penelitian: Penelitian ini merupakan penelitian pre experimental dengan pre test-post test control group design. Subjek penelitian yang diambil adalah semua ibu post partum yang melahirkan di Wilayah Kerja Puskesmas Temindung pada bulan Agustus-Oktober 2019. Sampel terdiri dar 2 kelompok dengan 48 responden, yang dipilih menggunakan teknik consecutive sampling. Analisis statistik uji t independen dengan tingkat signifikansi 0,05. Hasil: Hasil penelitian menunjukan perbedaan yang signifikan dari penurunan TFU pada ibu post partum antara kelompok yang melakukan senam nifas dan tidak senam nifas di Wilayah Kerja Puskesmas Temindung dibuktikan dengan p-value = 0,002. Kesimpulan dan saran: Pada penelitian ini Ho ditolak yang menunjukan bahwa ada pengaruh senam nifas terhadap penurunan tinggi fundus uteri pada ibu post partum di Klinik Kartika Jaya. Diharapkan senam nifas di terapkan oleh Wilayah Kerja Puskesmas Temindung karena bermanfaat dalam proses pemulihan kondisi ibu pasca partus.